Search results for: (5S)-N-(5-Amino-1-carboxypentyl)iminodiacetic Acid C10H18N2O6 CAS: 113231-05-3
#29044890 2017/10/18 Save this To Up
Overexpression of Serine Acetyltransferase in Maize Leaves Increases Seed-Specific Methionine-Rich Zeins.Maize kernels do not contain enough of the essential sulfur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulfur (S)-amino acids may limit their incorporation into seed storage proteins. Serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis and SAT overexpression is known to enhance S-assimilation without negative impact on plant growth. Therefore, we overexpressed Arabidopsis thaliana AtSAT1 in maize under control of the leaf bundle-sheath-cell-specific rbcS1 promoter to determine the impact on seed storage protein expression. The transgenic events exhibited up to 12-fold higher SAT activity without negative impact on growth. S-assimilation was increased in the leaves of SAT overexpressing plants, followed by higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa δ-zein, during endosperm development. This zein is known to impact the level of Met stored in kernels. The elite event with the highest expression of AtSAT1 showed 1.40 fold increase in kernel Met. When fed to chickens, transgenic AtSAT1 kernels significantly increased growth rate compared with the parent maize line. The result demonstrates the efficacy of increasing maize nutritional value by SAT overexpression without apparent yield loss. Maternal overexpression of SAT in vegetative tissues was necessary for high-Met zein accumulation. Moreover, SAT overcomes the shortage of S-amino acids that limits the expression and accumulation of high Met-zeins during kernel development. This article is protected by copyright. All rights reserved.
1044 related Products with: Overexpression of Serine Acetyltransferase in Maize Leaves Increases Seed-Specific Methionine-Rich Zeins.Rat Visceral adipose spec CAL-101 Mechanisms: PI3K- BYL-719 Mechanisms: PI3K- GSK-2636771 Mechanisms: P IPI-145 (INK-1197) Mechan Apoptosis antibody array Cell cycle antibody array Cytokine antibody array i Signal transduction antib AKT Phospho-Specific Arra AKT PKB Signaling Phospho AMPK Signaling Phospho-Sp
#29040832 2017/10/17 Save this To Up
Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics.Magnetic nanoparticles offer multiple possibilities for biomedical applications. Besides their physico-chemical properties, nanoparticle-cellular interactions are determinant for biological safety. In this work, magnetic nanoparticles were synthesized by one-shot precipitation or two-step reaction and coated with biocompatible polymers, such as poly(l-lysine) and poly(N,N-dimethylacrylamide-co-acrylic acid), and carbohydrates, like l-ascorbic acid, d-galactose, d-mannose, and sucrose. The resulting magnetic nanoparticles were characterized by dynamic light scattering, FT-Raman spectroscopy, transmission electron microscopy, SQUID magnetometry, and Mössbauer spectroscopy. Ability of the nanoparticles to be used in theranostic applications was also evaluated, showing that coating with biocompatible polymers increased the heating efficiency. Nanoparticles synthesized by one-shot precipitation were 50% larger (∼13nm) than those obtained by a two-step reaction (∼8nm). Magnetic nanoparticles at concentrations up to 500μgmL(-1) were non-cytotoxic to L929 fibroblasts. Particles synthesized by one-shot precipitation had little effect on viability, cell cycle and apoptosis of the three human colon cancer cell lines used: Caco-2, HT-29, and SW-480. At the same concentration (500μgmL(-1)), magnetic particles prepared by a two-step reaction reduced colon cancer cell viability by 20%, affecting cell cycle and inducing cell apoptosis. Uptake of surface-coated magnetic nanoparticles by colon cancer cells was dependent on particle synthesis, surface coating and incubation time.
2578 related Products with: Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics.MarkerGeneTM Fluorescent MarkerGene™ LysoLive™ Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Live Cell C Cell Meter™ Colorimetri
#29039868 2017/10/17 Save this To Up
Inkjet-printed barcodes for a rapid and multiplexed paper-based assay compatible with mobile devices.This study reports a simple, rapid, low-cost, robust, and multiplexed barcoded paper-based assay (BPA) compatible with mobile devices. An inkjet printer and an XYZ dispensing platform were used to realize mass-manufacturing of barcoded paper-based analytical devices (BPADs) with high precision and efficiency. We designed a new group of barcodes and developed an application (APP) for the reading of the new code. The new barcodes possess a 16 times higher coding capacity than the standard Codabar code in our experiment on drug residue detection. The BPA system allows applications in the assays of blood-transmitted infections, drug residues in milk and multiplex nucleic acids. The whole detection process and the readout of the results can be completed within 10 minutes. The limit of detection for enrofloxacin (ENR) (8 ng mL(-1)) satisfies the requirements of drug residue monitoring. Its high rapidity, simplicity, efficiency and selectivity make the BPA system extremely suitable to be applied in rapid and on-site detection.
2305 related Products with: Inkjet-printed barcodes for a rapid and multiplexed paper-based assay compatible with mobile devices.MarkerGeneTM Fluorescent Rapid Microplate Assay K Creatinine Assay Creatini Glucose Assay With the La Rapid collagenase assay k Rapid collagenase assay k Cultrex In Vitro Angiogen Endothelial Tube Formatio QuantiChrom™ LDH Cytoto QuantiChrom™ Formaldehy QuantiChrom™ Formaldehy EnzyChrom™ Kinase Assay
#29037664 2017/10/17 Save this To Up
Simultaneous optimization of the ultrasound-assisted extraction for phenolic compounds content and antioxidant activity of Lycium ruthenicum Murr. fruit using response surface methodology.Lycium ruthenicum Murr. (LR) is a functional food that plays an important role in anti-oxidation due to its high level of phenolic compounds. This study aims to optimize ultrasound-assisted extraction (UAE) of phenolic compounds and antioxidant activities of obtained extracts from LR using response surface methodology (RSM). A four-factor-three-level Box-Behnken design (BBD) was employed to discuss the following extracting parameters: extraction time (X1), ultrasonic power (X2), solvent to sample ratio (X3) and solvent concentration (X4). The analysis of variance (ANOVA) results revealed that the solvent to sample ratio had a significant influence on all responses, while the extraction time had no statistically significant effect on phenolic compounds. The optimum values of the combination of phenolic compounds and antioxidant activities were obtained for X1=30min, X2=100W, X3=40mL/g, and X4=33% (v/v). Five phenolic acids, including chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid and ferulic acid, were analyzed by HPLC. Our results indicated that optimization extraction is vital for the quantification of phenolic compounds and antioxidant activity in LR, which may be contributed to large-scale industrial applications and future pharmacological activities research.
1208 related Products with: Simultaneous optimization of the ultrasound-assisted extraction for phenolic compounds content and antioxidant activity of Lycium ruthenicum Murr. fruit using response surface methodology.CAR,CAR,Constitutive acti MarkerGene™ LysoLive™ Ofloxacin CAS Number [824 Cellufine Formyl , 50 ml Cellufine Formyl Media Cellufine Formyl , 500 ml Cellufine Formyl Media Cellufine Formyl Media Rapid Microplate Assay K Formalin Solution (20%) Formalin Solution (20%) Formalin Solution (20%)
#29036693 2017/10/16 Save this To Up
ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization.Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable expression measurement. Here, we present ICG (http://icg.big.ac.cn), a wiki-driven knowledgebase for community curation of experimentally validated internal control genes as well as their associated experimental conditions. Unlike extant related databases that focus on qPCR primers in model organisms (mainly human and mouse), ICG features harnessing collective intelligence in community integration of internal control genes for a variety of species. Specifically, it integrates a comprehensive collection of more than 750 internal control genes for 73 animals, 115 plants, 12 fungi and 9 bacteria, and incorporates detailed information on recommended application scenarios corresponding to specific experimental conditions, which, collectively, are of great help for researchers to adopt appropriate internal control genes for their own experiments. Taken together, ICG serves as a publicly editable and open-content encyclopaedia of internal control genes and accordingly bears broad utility for reliable RT-qPCR normalization and gene expression characterization in both model and non-model organisms.
1796 related Products with: ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization.EZH2 KMT6 Control Peptid GFP control peptide anti MOUSE ANTI BOVINE ROTAVIR Bone Morphogenetic Protei Growth Differentiation Fa HIV1-RT antibody, Monoclo HIV1-RT antibody, Monoclo HIV1-RT antibody, Monoclo Amplite™ Fluorimetric F FMK Negative Control ; Ap FMK Negative Control ; Ap QVD-OPh Negative Control;
#29036676 2017/10/16 Save this To Up
Anti-CRISPRdb: a comprehensive online resource for anti-CRISPR proteins.CRISPR-Cas is a tool that is widely used for gene editing. However, unexpected off-target effects may occur as a result of long-term nuclease activity. Anti-CRISPR proteins, which are powerful molecules that inhibit the CRISPR-Cas system, may have the potential to promote better utilization of the CRISPR-Cas system in gene editing, especially for gene therapy. Additionally, more in-depth research on these proteins would help researchers to better understand the co-evolution of bacteria and phages. Therefore, it is necessary to collect and integrate data on various types of anti-CRISPRs. Herein, data on these proteins were manually gathered through data screening of the literatures. Then, the first online resource, anti-CRISPRdb, was constructed for effectively organizing these proteins. It contains the available protein sequences, DNA sequences, coding regions, source organisms, taxonomy, virulence, protein interactors and their corresponding three-dimensional structures. Users can access our database at http://cefg.uestc.edu.cn/anti-CRISPRdb/ without registration. We believe that the anti-CRISPRdb can be used as a resource to facilitate research on anti-CRISPR proteins and in related fields.
2910 related Products with: Anti-CRISPRdb: a comprehensive online resource for anti-CRISPR proteins.MOUSE ANTI BOVINE ROTAVIR Mouse Anti-RSV 33kDa & 19 MOUSE ANTI BORRELIA BURGD Anti Galectin(Gal 3) Huma Anti AGE 3 Monoclonal Ant RABBIT ANTI GSK3 BETA (pS Rat Anti-Mouse Forssman G Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H
#29036650 2017/10/16 Save this To Up
Characteristic arrangement of nucleosomes is predictive of chromatin interactions at kilobase resolution.High-throughput chromosome conformation capture (3C) technologies, such as Hi-C, have made it possible to survey 3D genome structure. However, obtaining 3D profiles at kilobase resolution at low cost remains a major challenge. Therefore, we herein present an algorithm for precise identification of chromatin interaction sites at kilobase resolution from MNase-seq data, termed chromatin interaction site detector (CISD), and a CISD-based chromatin loop predictor (CISD_loop) that predicts chromatin-chromatin interactions (CCIs) from low-resolution Hi-C data. We show that the predictions of CISD and CISD_loop overlap closely with chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) anchors and loops, respectively. The validity of CISD/CISD_loop was further supported by a 3C assay at about 5 kb resolution. Finally, we demonstrate that only modest amounts of MNase-seq and Hi-C data are sufficient to achieve ultrahigh resolution CCI maps. Our results suggest that CCIs may result in characteristic nucleosomes arrangement patterns flanking the interaction sites, and our algorithms may facilitate precise and systematic investigations of CCIs on a larger scale than hitherto have been possible.
1234 related Products with: Characteristic arrangement of nucleosomes is predictive of chromatin interactions at kilobase resolution.V-ATPase 116 kDa isoform Atorvastatin Cyclic Sodiu Allyl Ester of Atorvastat Atorvastatin 3-Isopropyl cis-Atovaquone-d5 (contai Rabbit Anti-ATP1b2 Na+K+A Rabbit Anti-ATP1b2 Na+K+A Rabbit Anti-ATP1b2 Na+K+A Rabbit Anti-ATP1b2 Na+K+A Rabbit Anti-ATP1b2 Na+K+A Rabbit Anti-ATP1b2 Na+K+A Rabbit Anti-ATM Polyclona
#29036456 2017/10/16 Save this To Up
Role of free DNA ends and protospacer adjacent motifs for CRISPR DNA uptake in Pyrococcus furiosus.To acquire CRISPR-Cas immunity against invasive mobile genetic elements, prokaryotes must first integrate fragments of foreign DNA into their genomic CRISPR arrays for use in future invader silencing. Here, we found that the hyperthermophilic archaeaon, Pyrococcus furiosus, actively incorporates DNA fragments (spacers) from both plasmid (foreign) and host genome (self) sequences into its seven CRISPR loci. The majority of new spacers were derived from DNA immediately downstream from a 5'-CCN-3' protospacer adjacent motif (PAM) that is critical for invader targeting. Interestingly, spacers were preferentially acquired from genome or plasmid regions corresponding to active transposons, CRISPR loci, ribosomal RNA genes, rolling circle origins of replication, and areas where plasmids recombined with the host chromosome. A common feature of the highly sampled spacers is that they arise from DNA regions expected to undergo DNA nicking and/or double-strand breaks. Taken together with recent results from bacterial systems, our findings indicate that free DNA termini and PAMs are conserved features important for CRISPR spacer uptake in diverse prokaryotes and CRISPR-Cas systems. Moreover, lethal self-targeting by CRISPR systems may contribute to host genome stability by eliminating cells undergoing active transposon mobility or chromosomal uptake of autonomously replicating foreign mobile genetic elements.
1882 related Products with: Role of free DNA ends and protospacer adjacent motifs for CRISPR DNA uptake in Pyrococcus furiosus.Pfu DNA Polymerase protei Protease, DNASE free hea Protease, DNASE free hea Protease, DNASE free hea DNA (cytosine 5) methyltr removed without changing Protease, DNASE free heat Protease, DNASE free heat Protease, DNASE free heat Protease, DNASE free heat DNA kits, Column-free Iso Pfu DNA Polymerase (Not a
#29036323 2017/10/16 Save this To Up
Structural basis for the specific recognition of 18S rRNA by APUM23.PUF (Pumilio/fem-3 mRNA binding factor) proteins, a conserved family of RNA-binding proteins, recognize specific single-strand RNA targets in a specific modular way. Although plants have a greater number of PUF protein members than do animal and fungal systems, they have been the subject of fewer structural and functional investigations. The aim of this study was to elucidate the involvement of APUM23, a nucleolar PUF protein in the plant Arabidopsis, in pre-rRNA processing. APUM23 is distinct from classical PUF family proteins, which are located in the cytoplasm and bind to 3'UTRs of mRNA to modulate mRNA expression and localization. We found that the complete RNA target sequence of APUM23 comprises 11 nt in 18S rRNA at positions 1141-1151. The complex structure shows that APUM23 has 10 PUF repeats; it assembles into a C-shape, with an insertion located within the inner concave surface. We found several different RNA recognition features. A notable structural feature of APUM23 is an insertion in the third PUF repeat that participates in nucleotide recognition and maintains the correct conformation of the target RNA. Our findings elucidate the mechanism for APUM23's-specific recognition of 18S rRNA.
MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD NATIVE HUMAN PROLACTIN, P BYL-719 Mechanisms: PI3K- RABBIT ANTI GSK3 BETA (pS 10x ELISA WASH BUFFER, Pr 10X PHOSPHATE BUFFERED SA PERMANENT AQUEOUS MOUNTIN Multiple organ cancer tis Multiple organ tumor tiss MOUSE ANTI CANINE DISTEMP MOUSE ANTI HUMAN CD15, Pr
#29035497 2017/10/16 Save this To Up
Conformational Dynamics of DNA Binding and Cas3 Recruitment by the CRISPR RNA-guide Cascade Complex.Bacteria and archaea rely on CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided adaptive immune systems for sequence specific elimination of foreign nucleic acids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble with Cas (CRISPR-associated) proteins into a 405-kilodalton multi-subunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Cascade binds foreign DNA complementary to the crRNA guide and recruits Cas3, a trans-acting nuclease-helicase required for target degradation. Structural models of Cascade have captured static snapshots of the complex in distinct conformational states, but conformational dynamics of the 11-subunit surveillance complex have not been measured. Here we use hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) to map conformational dynamics of Cascade onto the three-dimensional structure. New insights from structural dynamics are used to make functional predictions about the mechanisms of the R-loop coordination and Cas3 recruitment. We test these predictions in vivo and in vitro. Collectively, we show how mapping conformational dynamics onto static 3D-structures adds an additional dimension to the functional understanding of this biological machine.
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