Gentaur Pub

#38652440   2024/04/23 To Up

CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.

The development of a bio-sensing strategy based on CRISPR/Cas that is exceptionally sensitive is crucial for the identification of trace molecules. Colorimetric miRNA detection utilizing CRISPR/Cas13a-triggered DNAzyme signal amplification was described in this article. The developed strategy was implemented for miRNA-21 detection as a proof of concept. The cleavage activity of Cas13a was triggered when the target molecule bonded to the Cas13a-crRNA complex and cleaved uracil ribonucleotides (rU) in the substrate probe. As a consequence, the S chain was liberated from the T chain that had been modified on magnetic beads (MB). The G-rich sections were then exposed when the catalytic hairpin assembly between the H1 and H2 probes was activated by the released T@MB. G-rich section can fold into G-quadruplex. By catalyzing the formation of green ABTS- via HRP-mimicking G-quadruplex/hemin complexes, colorimetric measurements of miRNA can be achieved visually through DNAzyme-mediated signal amplification. The method demonstrated a low limit of detection of 27 fM and a high selectivity towards target miRNA eventually. As a result, the developed strategy provides a clinical application platform for the detection of miRNAs that is both ultrasensitive and extremely specific.
Na Yan, Zhiwei Hu, Lingling Zhang

1770 related Products with: CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.

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#38652321   2024/04/23 To Up

Advances in miniature CRISPR-Cas proteins and their applications in gene editing.

T
Huimin Wu, Yixiang Sun, Yimai Wang, Liqiang Luo, Yizhi Song

2596 related Products with: Advances in miniature CRISPR-Cas proteins and their applications in gene editing.

101 Set100 7 x 25 ul

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#38652294   2024/04/23 To Up

Plant diversity increases spatial stability of aboveground productivity in alpine grasslands.

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Zeng Cui, Jian Sun, Gao-Lin Wu

1812 related Products with: Plant diversity increases spatial stability of aboveground productivity in alpine grasslands.

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#38652293   2024/04/23 To Up

Intraspecific alternative phenotypes contribute to variation in species' strategies for growth.

Ecologists have historically sought to identify the mechanisms underlying the maintenance of local species diversity. High-dimensional trait-based relationships, such as alternative phenotypes, have been hypothesized as important for maintaining species diversity such that phenotypically dissimilar individuals compete less for resources but have similar performance in a given environment. The presence of alternative phenotypes has primarily been investigated at the community level, despite the importance of intraspecific variation to diversity maintenance. The aims of this research are to (1) determine the presence or absence of intraspecific alternative phenotypes in three species of tropical tree seedlings, (2) investigate if these different species use the same alternative phenotypes for growth success, and (3) evaluate how findings align with species co-occurrence patterns. We model species-specific relative growth rate with individual-level measurements of leaf mass per area (LMA) and root mass fraction (RMF), environmental data, and their interactions. We find that two of the three species have intraspecific alternative phenotypes, with individuals within species having different functional forms leading to similar growth. Interestingly, individuals within these species use the same trait combinations, high LMA low RMF and low LMA high RMF, in high soil nutrient environments to acquire resources for higher growth. This similarity among species in intraspecific alternative phenotypes and variables that contribute most to growth may lead to their negative spatial co-occurrence. Overall, we find that multiple traits or interactions between traits and the environment drive species-specific strategies for growth, but that individuals within species leverage this multi-dimensionality in different ways for growth success.
Samantha J Worthy, María N Umaña, Caicai Zhang, Luxiang Lin, Min Cao, Nathan G Swenson

1068 related Products with: Intraspecific alternative phenotypes contribute to variation in species' strategies for growth.

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#38652189   2024/04/23 To Up

Siderophore-producing bacteria from Spitsbergen soils-novel agents assisted in bioremediation of the metal-polluted soils.

Siderophores are molecules that exhibit a high specificity for iron (Fe), and their synthesis is induced by a deficiency of bioavailable Fe. Complexes of Fe-siderophore are formed extracellularly and diffuse through porins across membranes into bacterial cells. Siderophores can bind heavy metals facilitating their influx into cells via the same mechanism. The aim of the studies was to determine the ability of siderophore-producing bacteria isolated from soils in the north-west part of Wedel Jarlsberg Land (Spitsbergen) to chelate non-Fe metals (Al, Cd, Co, Cu, Hg, Mn, Sn, and Zn). Specially modified blue agar plates were used, where Fe was substituted by Al, Cd, Co, Cu, Hg, Mn, Sn, or Zn in metal-chrome azurol S (CAS) complex, which retained the blue color. It has been proven that 31 out of 33 strains were capable of producing siderophores that bind to Fe, as well as other metals. Siderophores from Pantoea sp. 24 bound only Fe and Zn, and O. anthropi 55 did not produce any siderophores in pure culture. The average efficiency of Cd, Co, Cu, Mn, Sn, and Zn chelation was either comparable or higher than that of Fe, while Al and Hg showed significantly lower efficiency. Siderophores produced by S. maltophilia 54, P. luteola 27, P. luteola 46, and P. putida 49 exhibited the highest non-Fe metal chelation activity. It can be concluded that the siderophores of these bacteria may constitute an integral part of the metal bioleaching preparation, and this fact will be the subject of further research.
Małgorzata Majewska, Anna Słomka, Agnieszka Hanaka

2599 related Products with: Siderophore-producing bacteria from Spitsbergen soils-novel agents assisted in bioremediation of the metal-polluted soils.

1

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#38652094   2024/04/23 To Up

Sulfonic Acid Functionalized Ionic Liquids for Defect Passivation via Molecular Interactions for High-Quality Perovskite Films and Stable Solar Cells.

The high photoelectric conversion efficiency and low cost of perovskite solar cells (PSCs) have further inspired people's determination to push this technology toward industrialization. The high-quality perovskite films and high-efficiency and stable PSCs are the crucial factors. Ionic liquids have been proven to be an effective strategy for regulating high-quality perovskite films and high-performance PSCs. However, the regulation mechanism between ionic liquids and perovskites still needs further clarification. In this study, a novel sulfonic acid-functionalized ionic liquid, 1-butyl-3-methylimidazolium trifluoromethanesulfonate (BSOHMImOTf), was used as an effective additive to regulate high-quality perovskite films and high-performance devices. Microscopic mechanism studies revealed strong interactions between BSOHMImOTf and Pb ions as well as halogens in the perovskite. The perovskite film is effectively passivated with the controlled crystal growth, suppressed ion migration, facilitating to the greatly improved photovoltaic performance, and superior long-term stability. This article reveals the regulatory mechanism of sulfonic acid type ionic liquids through testing characterization and mechanism analysis, providing a new approach for the preparation of high-quality perovskite devices.
Junhui Fang, Liang Wang, Zhaoyang Chen, Shuai Wang, Lin Yuan, Aamir Saeed, Iqbal Hussain, Jianwei Zhao, Ruixia Liu, Qingqing Miao

1548 related Products with: Sulfonic Acid Functionalized Ionic Liquids for Defect Passivation via Molecular Interactions for High-Quality Perovskite Films and Stable Solar Cells.

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#38651920   2024/04/23 To Up

Biomechanical effects of clear aligner with different shape design at extraction space area during anterior teeth retraction.

This study aimed to investigate the biomechanical effects of clear aligner (CA) with different shape designs at extraction space (CAES) area during space closing.
Bochun Mao, Yajing Tian, Yujia Xiao, Jiayi Liu, Dawei Liu, Jing Li, Yanheng Zhou

1525 related Products with: Biomechanical effects of clear aligner with different shape design at extraction space area during anterior teeth retraction.

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#38651836   2024/04/23 To Up

Machine Learning for Experimental Reactivity of a Set of Metal Clusters toward C-H Activation.

U
Xi-Guan Zhao, Qi Yang, Ying Xu, Qing-Yu Liu, Zi-Yu Li, Xiao-Xiao Liu, Yan-Xia Zhao, Sheng-Gui He

1773 related Products with: Machine Learning for Experimental Reactivity of a Set of Metal Clusters toward C-H Activation.

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#38651620   2024/04/23 To Up

Acceptor-Reactivity-Controlled Stereoconvergent Synthesis and Immunological Activity of a Unique Pentasaccharide from the Cell Wall Polysaccharide of Cutibacterium acnes C7.

Bacterial cell-surface polysaccharides are involved in various biological processes and have attracted widespread attention as potential targets for developing carbohydrate-based drugs. However, the accessibility of structurally well-defined polysaccharide or related active oligosaccharide domains remains challenging. Herein, we describe an efficiently stereocontrolled approach for the first total synthesis of a unique pentasaccharide repeating unit containing four difficult-to-construct 1,2-cis-glycosidic linkages from the cell wall polysaccharide of Cutibacterium acnes C7. The features of our approach include: 1) acceptor-reactivity-controlled glycosylation to stereoselectively construct   two challenging rare 1,2-cis-ManA2,3(NAc)2 (β-2,3-diacetamido-2,3-dideoxymannuronic acid) linkages, 2) combination use of 6-O-tert-butyldiphenylsilyl (6-O-TBDPS)-mediated steric shielding effect and ether solvent effect to stereoselectively install a 1,2-cis-glucosidic linkage, 3) bulky 4,6-di-O-tert-butylsilylene (DTBS)-directed glycosylation to stereospecifically construct a 1,2-cis-galactosidic linkage, 4) stereoconvergent [2+2+1] and one-pot chemoselective glycosylation to rapidly assemble the target pentasaccharide. Immunological activity tests suggest that the pentasaccharide can induce the production of proinflammatory cytokine TNF-α in a dose-dependent manner.
Tianhui Hao, Ke Feng, Hongzhen Jin, Jiawei Li, Chenkai Zhou, Xingbang Liu, Wei Zhao, Fan Yu, Tiehai Li

2046 related Products with: Acceptor-Reactivity-Controlled Stereoconvergent Synthesis and Immunological Activity of a Unique Pentasaccharide from the Cell Wall Polysaccharide of Cutibacterium acnes C7.

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#38651538   2024/04/23 To Up

A wafer scale thin film of ultra-small ScO nanocrystals on a 2D COF with high rigidity.

Scandium oxide (ScO) has a wide range of applications in metallurgy, chemical industry, electronics and many other high-tech fields. However, most ScO materials exist in the powder or bulk form, while nanostructured ScO has rarely been reported as there is a lack of a common method to control its dimensionality, hindering the understanding of new properties and potential applications of nano-ScO materials. In this paper, we establish a procedure to synthesize a two-dimensional (2D) ScO-covalent organic framework (COF) composite film where the crystal size of ScO domains is as small as ∼3 nm. The composite film is prepared by a Schiff base condensation reaction at the sharp -pentane/water interface using a combination of surfactant-monolayer-assisted interfacial synthesis and laminar assembly polymerization methods. Then the conditions of nucleation and uniform film formation of the 2D ScO/COF are explored further. Meanwhile, an atomic force microscopy indentation test shows that the material has a high Young's modulus of 89.1 ± 3.8 GPa, which is much higher than those of the majority of reported 2D polymer materials. We further extended this synthesis method to the preparation of YbO (ytterbium oxide) and/or ErO (erbium oxide)-incorporated 2D COF composite films, verifying the universality of this strategy. This work provides an opportunity to vary the dimensionality of many kinds of metal oxides and explore the potential applications of low-dimensional ScO materials.
Xin Guan, Xiaohui Xu, Zhongliang Yu, Junjie Xiong, Yanhong Chang, Bowen Liu, Bin Wang

2504 related Products with: A wafer scale thin film of ultra-small ScO nanocrystals on a 2D COF with high rigidity.

100ug Lyophilized2 Pieces/Box4 Sample Kit50 ul1000 assays4 Arrays/Slide

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