Search results for: 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-d3 C11H8D3N5 CAS: 122457-31-2
#38648296 
2024/04/22
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Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.
TTianli Luo, Qizhen Zheng, Ji Liu, Rui Yao, Ming Wang
1213 related Products with: Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.
501mg101mg1 mg1001010021.00 flask
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#38648198 2024/04/22
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The impact of phage and phage resistance on microbial community dynamics.
WEllinor O Alseth, Rafael Custodio, Sarah A Sundius, Rachel A Kuske, Sam P Brown, Edze R Westra
2699 related Products with: The impact of phage and phage resistance on microbial community dynamics.
0.25 mL100ug100ug 6 ml 100 mg25 mg10 25 MG
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#38648173 2024/04/22
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How to use CRISPR/Cas9 in plants - from target site selection to DNA repair.
A tool for precise, target-specific, efficient and affordable genome editing, it is a dream for many researchers, from those who do basic research to those who use it for applied research. Since 2012, we have the tool that almost fulfils such requirements; it is based on CRISPR/Cas systems. However, even CRISPR/Cas has limitations and obstacles that might surprise its users. In this review, we focus on the most frequently used variant, CRISPR/Cas9 from Streptococcus pyogenes, and highlight the key factors affecting its mutagenesis outcomes. Firstly, factors affecting the CRISPR/Cas9 activity, such as the effect of the target sequence, chromatin state or Cas9 variant, and how long it remains in place after cleavage. Secondly, factors affecting the follow-up DNA repair mechanisms include mostly the cell type and cell cycle phase, but also, for example, the type of DNA ends produced by Cas9 cleavage (blunt/staggered). Moreover, we note some differences between using CRISPR/Cas9 in plants, yeasts and animals, as knowledge from individual kingdoms is not fully transferable. Awareness of these factors can increase the likelihood of achieving the expected results of plant genome editing, for which we provide detailed guidelines.Adéla Přibylová, Lukáš Fischer
2473 related Products with: How to use CRISPR/Cas9 in plants - from target site selection to DNA repair.
100 0.1ml (1.3mg/ml)100 1 mg500 gm.10 1 mg
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#38647893 2022/07/30
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Production of free fatty acids from various carbon sources by Ogataea polymorpha.
EYunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou
1612 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.
1,000 tests100tests1001KG500gm1kg100gm500g50gm
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#38647886 2023/06/28
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Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.
It is of great significance to utilize CO as feedstock to synthesize biobased products, particularly single cell protein (SCP) as the alternative food and feed. Bioelectrochemical system (BES) driven by clean electric energy has been regarded as a promising way for Cupriavidus necator to produce SCP from CO directly. At present, the key problem of culturing C. necator in BES is that reactive oxygen species (ROS) generated in cathode chamber are harmful to bacterial growth. Therefore, it is necessary to find a solution to mitigate the negative effect of ROS. In this study, we constructed a number of C. necator strains displayed with superoxide dismutase (SOD), which allowed the decomposition of superoxide anion radical. The effects of promoters and signal peptides on the cell surface displayed SOD were analyzed. The proteins displayed on the surface were further verified by the fluorescence experiment. Finally, the growth of C. necator CMS incorporating a pBAD-SOD-E-tag-IgAβ plasmid could achieve 4.9 ± 1.0 of OD by 7 days, equivalent to 1.7 ± 0.3 g/L dry cell weight (DCW), and the production rate was 0.24 ± 0.04 g/L/d DCW, around 2.7-fold increase than the original C. necator CMS (1.8 ± 0.3 of OD). This study can provide an effective and novel strategy of cultivating strains for the production of CO-derived SCP or other chemicals in BES.Ke Chen, Chunling Ma, Xiaolei Cheng, Yuhua Wang, Kun Guo, Ranran Wu, Zhiguang Zhu
2065 related Products with: Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.
20ug100.00 ug500 MG2 Pieces/Box10ug100.00 ug0.1ml (1mg/ml)10ug 100 G100.00 ug250 mg
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#38647878 2023/03/02
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Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.
LShiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K Zhao
2747 related Products with: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.
1 G 1 G100 mg300 units 5 G10 mg
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#38647822 2022/05/26
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Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.
α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou
2781 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.
1 mg100 µg1 mL100ug100ug6 ml100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg
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#38647771 2022/10/22
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Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.
IYunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An
1946 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.
1 mg100μg0.1ml (1mg/ml)500 500 500 μg100ug100ug Lyophilized100ug1 mL100ug Lyophilized100μg
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#38647747 2022/05/18
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A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.
To mimic the Escherichia coli T7 protein expression system, we developed a facile T7 promoter-based protein expression system in an industrial microorganism Bacillus subtilis. This system has two parts: a new B. subtilis strain SCK22 and a plasmid pHT7. To construct strain SCK22, the T7 RNA polymerase gene was inserted into the chromosome, and several genes, such as two major protease genes, a spore generation-related gene, and a fermentation foam generation-related gene, were knocked out to facilitate good expression in high-density cell fermentation. The gene of a target protein can be subcloned into plasmid pHT7, where the gene of the target protein was under tight control of the T7 promoter with a ribosome binding site (RBS) sequence of B. subtilis (i.e., AAGGAGG). A few recombinant proteins (i.e., green fluorescent protein, α-glucan phosphorylase, inositol monophosphatase, phosphoglucomutase, and 4-α-glucanotransferase) were expressed with approximately 25-40% expression levels relative to the cellular total proteins estimated by SDS-PAGE by using B. subtilis SCK22/pHT7-derived plasmid. A fed-batch high-cell density fermentation was conducted in a 5-L fermenter, producing up to 4.78 g/L inositol monophosphatase. This expression system has a few advantageous features, such as, wide applicability for recombinant proteins, high protein expression level, easy genetic operation, high transformation efficiency, good genetic stability, and suitability for high-cell density fermentation.Jing Ye, Yunjie Li, Yuqing Bai, Ting Zhang, Wei Jiang, Ting Shi, Zijian Wu, Yi-Heng P Job Zhang