Gentaur Pub

#38648296   2024/04/22 To Up

Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.

The delivery of proteins into the cytosol holds great promise for cell signaling manipulation and the development of precision medicine. However, this potency is challenged by achieving targeted and controlled delivery, specifically within diseased cells. In this study, we introduce a versatile and effective method for the precision delivery of therapeutic proteins to cancer cells by designing polyphenol-assisted biomineralization of zeolite imidazole framework-8 (ZIF-8). We demonstrate that by leveraging the strong noncovalent binding affinity of epigallocatechin gallate (EGCG) with both proteins and ZIF-8, our approach significantly enhances the biomineralization of ZIF-8, which in turn improves the efficiency of protein encapsulation and intracellular delivery. Moreover, the incorporation of EGCG within ZIF-8 enables controlled degradation of the nanoparticles and the selective release of the encapsulated proteins in cancer cells. This selective release is triggered by the oxidation of EGCG in response to the high levels of reactive oxygen species (ROS) found within cancer cells that destabilize the EGCG/ZIF-8 nanoparticles. We have further demonstrated the ability of EGCG/ZIF-8 to deliver a wide range of proteins into cancer cells, including bacterial virulence protein, to rewire cell signaling and prohibit tumor cell growth in a mouse xenograft model. Our strategy and findings underscore the potential of designing the EGCG/ZIF-8 interface for specific and controlled protein delivery for targeted cancer therapy.
Tianli Luo, Qizhen Zheng, Ji Liu, Rui Yao, Ming Wang

1099 related Products with: Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.

10501mg1mg1 mg1002101.00 flask100

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#38648198   2024/04/22 To Up

The impact of phage and phage resistance on microbial community dynamics.

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Ellinor O Alseth, Rafael Custodio, Sarah A Sundius, Rachel A Kuske, Sam P Brown, Edze R Westra

1178 related Products with: The impact of phage and phage resistance on microbial community dynamics.

0.25 mL 25 MG1000 tests100ug100ug 6 ml 100 mg25 mg

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#38648173   2024/04/22 To Up

How to use CRISPR/Cas9 in plants - from target site selection to DNA repair.

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Adéla Přibylová, Lukáš Fischer

1493 related Products with: How to use CRISPR/Cas9 in plants - from target site selection to DNA repair.

0.1ml (1.3mg/ml)100 100 1 mg500 gm.10 1 mg

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#38647965   2023/09/15 To Up

Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.

In the context of the rapid development of low-carbon economy, there has been increasing interest in utilizing naturally abundant and cost-effective one-carbon (C1) substrates for sustainable production of chemicals and fuels. Moorella thermoacetica, a model acetogenic bacterium, has attracted significant attention due to its ability to utilize carbon dioxide (CO) and carbon monoxide (CO) via the Wood-Ljungdahl (WL) pathway, thereby showing great potential for the utilization of C1 gases. However, natural strains of M. thermoacetica are not yet fully suitable for industrial applications due to their limitations in carbon assimilation and conversion efficiency as well as limited product range. Over the past decade, progresses have been made in the development of genetic tools for M. thermoacetica, accelerating the understanding and modification of this acetogen. Here, we summarize the physiological and metabolic characteristics of M. thermoacetica and review the recent advances in engineering this bacterium. Finally, we propose the future directions for exploring the real potential of M. thermoacetica in industrial applications.
Dechen Jia, Wangshuying Deng, Peng Hu, Weihong Jiang, Yang Gu

2989 related Products with: Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.

0.2 mg10 mg250 ml5 g25 mg10 mg0.1 mg1 mg10 mg1 ml250 mg1 LITRE

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#38647893   2022/07/30 To Up

Production of free fatty acids from various carbon sources by Ogataea polymorpha.

Energy shortage and environmental concern urgently require establishing the feasible bio-refinery process from various feedstocks. The methylotrophic yeast Ogataea polymorpha is thermo-tolerant and can utilize various carbon sources, such as glucose, xylose and methanol, which makes it a promising host for bio-manufacturing. Here, we explored the capacity of O. polymorpha for overproduction of free fatty acids (FFAs) from multiple substrates. The engineered yeast produced 674 mg/L FFA from 20 g/L glucose in shake flask and could sequentially utilize the mixture of glucose and xylose. However, the FFA producing strain failed to survive in sole methanol and supplementing co-substrate xylose promoted methanol metabolism. A synergistic utilization of xylose and methanol was observed in the FFA producing strain. Finally, a mixture of glucose, xylose and methanol was evaluated for FFA production (1.2 g/L). This study showed that O. polymorpha is an ideal host for chemical production from various carbon sources.
Yunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou

1070 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.

1,000 tests100tests1001KG500gm1kg100gm500g50gm

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#38647878   2023/03/02 To Up

Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.

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Shiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K Zhao

1362 related Products with: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.

1 G 1 G100 mg300 units 5 G10 mg

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#38647822   2022/05/26 To Up

Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.
Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou

2644 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

1 mg100 µg1 mL100ug100ug100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg200

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#38647771   2022/10/22 To Up

Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

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Yunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An

1348 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

1 mg100μg0.1ml (1mg/ml)500 500 500 μg100ug100ug Lyophilized100ug1 mL100ug Lyophilized100μg

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#38647747   2022/05/18 To Up

A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.

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Jing Ye, Yunjie Li, Yuqing Bai, Ting Zhang, Wei Jiang, Ting Shi, Zijian Wu, Yi-Heng P Job Zhang

1291 related Products with: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.

25mg50 400Tests

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