Search results for: Acrolein-d4 DISCONTINUED C3D4O CAS: 33984-05-3
#29055163 2017/10/21 Save this To Up
Microbial sensor for drug susceptibility testing of Mycobacterium tuberculosis.Drug susceptibility testing (DST) of clinical isolates of Mycobacterium tuberculosis (M. tuberculosis) is critical in treating tuberculosis. We demonstrate the possibility of using a microbial sensor to perform DST of M. tuberculosis and shorten the time required for DST.
2838 related Products with: Microbial sensor for drug susceptibility testing of Mycobacterium tuberculosis.Mycobacterium tuberculosi Rabbit Polyclonal to Myco Mycobacterium Tuberculosi Cellufine Formyl , 50 ml Cellufine Formyl Media Cellufine Formyl , 500 ml Cellufine Formyl Media Cellufine Formyl Media Formalin Solution (20%) Formalin Solution (20%) Formalin Solution (20%) Formalin (10% Neutral Bu
#29054873 2017/10/21 Save this To Up
The enigmatic genome of an obligate ancient Spiroplasma symbiont in a hadal holothurian.Protective symbiosis has been reported in many organisms, but the molecular mechanisms of the mutualistic interactions between the symbionts and their host are unclear. Here, we sequenced the 424-Kbp genome of "Candidatus Spiroplasma holothuricola" that dominated the hindgut microbiome of a sea cucumber, a major scavenger captured in the Mariana trench (6140m depth). Phylogenetic relationships indicated that the dominant bacterium in the hindgut was derived from a basal group of Spiroplasma sp. In this organism, the genes responsible for biosynthesis of amino acids, glycolysis and sugar transporters were lost, strongly suggesting endosymbiosis. The highly decayed genome consists of two chromosomes and harbors genes coded for proteolysis, microbial toxin, restriction-methylation systems and clustered regularly interspaced short palindromic repeats (CRISPRs), composed of three cas genes and 76 CRISPR spacers. The holothurian host is probably protected against invading viruses from sediments by the CRISPRs/Cas and restriction systems of the endosymbiotic spiroplasma. The protective endosymbiosis indicates the important ecological role of the ancient Spiroplasma symbiont in maintenance of hadal ecosystems.IMPORTANCESea cucumbers are major inhabitants in hadal trenches. They collect microbes in surface sediment and remain tolerant against potential pathogenic bacteria and viruses. This study presents the genome of endosymbiotic spiroplasmas in the gut of sea cucumber captured in the Mariana trench. The extreme reduction of the genome and loss of essential metabolic pathways strongly support its endosymbiotic life mode. Moreover, a considerable part of the genome was occupied by CRISPR/Cas system to provide immunity against viruses and antimicrobial toxin-coding genes for degradation of microbes. This novel species of Spiroplasma is probably an important protective symbiont for the sea cucumbers in the hadal zone.
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#29054811 2017/10/21 Save this To Up
Molecular phylogeny of bladderworts: a wide approach of Utricularia (Lentibulariaceae) species relationships based on six plastidial and nuclear DNA sequences.The carnivorous plant genus Utricularia L. (bladderwort) comprises about 240 species distributed worldwide and is traditionally classified into two subgenera (Polypompholyx and Utricularia) and 35 sections, based mainly on general and trap morphology. It is one out of the largest carnivorous genera, representing ca. 30% of all carnivorous plant species, and is also the most widely distributed. According to previous phylogenetic studies, most infrageneric sections are monophyletic, but there are several incongruences considering their relationships and also the dissenting position of some species as a result of a too few (mostly one or two) molecular markers analyzed. Thus, here we present a multilocus phylogeny for Utricularia species with a wide taxonomic sampling (78 species and 115 accessions) based on six plastid (rbcL, matK, rpl20-rps12, rps16, trnL-F) and nuclear DNA (ITS region) sequences. The aim is to reconstruct a well-resolved tree to propose evolutionary and biogeographic hypotheses for the radiation of lineages with inferences about the divergence times of clades using a molecular clock approach.
1378 related Products with: Molecular phylogeny of bladderworts: a wide approach of Utricularia (Lentibulariaceae) species relationships based on six plastidial and nuclear DNA sequences.Androgen Receptor (Phosph Androgen Receptor (Phosph DNA PKcs (Phospho Thr2609 Androgen Receptor (Ab 650 DNA PKcs (Ab 2609) Antibo DNAI2 antibody Source Rab DNAJB2 antibody Source Ra DNAJC7 antibody Source Ra DNAJA2 antibody Source Ra DNA ligase 3 antibody Sou DNAJB6 antibody Source Ra DNA pol delta cat antibod
#29054725 2017/10/21 Save this To Up
Accelerometer-Based and Computer-Assisted Navigation in Total Knee Arthroplasty: A Reduction in Mechanical Axis Outliers Does Not Lead to Improvement in Functional Outcomes or Quality of Life When Compared to Conventional Total Knee Arthroplasty.Accelerometer-based navigation (ABN) is a novel navigation system that attempts to combine the accuracy of computer-assisted surgery (CAS) with the familiarity of conventional instrumentation (CON). No studies have compared the clinical outcomes of this new technology with existing techniques to date.
2611 related Products with: Accelerometer-Based and Computer-Assisted Navigation in Total Knee Arthroplasty: A Reduction in Mechanical Axis Outliers Does Not Lead to Improvement in Functional Outcomes or Quality of Life When Compared to Conventional Total Knee Arthroplasty.Cell Meter™ Fluorimetri FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Cell Meter™ Fluorimetri Oral squamous cell cancer ELISA Mouse , Interleukin Recombinant Human Interfe Homogenizer for 24 sample Top five cancer tissue ar Goat Anti-Human TOM1L1 SR
#29054643 2017/10/21 Save this To Up
Polycyclic aromatic hydrocarbons in the urban atmosphere of Nepal: Distribution, sources, seasonal trends, and cancer risk.Atmospheric polycyclic aromatic hydrocarbons (PAHs) in urban areas have always been a global concern, as these areas are considered to be the source region. Despite studies on the concentrations of PAHs in water, soils and sediments, knowledge of the distribution patterns, seasonality and sources of PAHs in urban areas of Nepal remains limited. In this study, polyurethane foam passive air samplers were used to measure gas-phase PAH concentrations over different land types in three major cities of Nepal-namely, Kathmandu (the capital) and Pokhara (both densely populated cities), and Hetauda (an agricultural city). The average concentrations of ∑15PAHs in ng/m(3) were 16.1±7.0 (6.4-28.6), 14.1±6.2 (6.8-29.4) and 11.1±9.0 (4.1-38.0) in Kathmandu, Pokhara and Hetauda, respectively. Molecular diagnostic ratio analysis suggested that fossil fuel combustion was a common PAH source for all three cities. In addition to this, coal combustion in Kathmandu, vehicle emissions in Pokhara, and grass/wood combustion in Hetauda were also possible sources of PAHs. In terms of cancer risk from PAH inhalation, a religious site with intense incense burning, a brick production area where extensive coal combustion is common, and a market place with heavy traffic emission, were associated with a higher risk than other areas. There were no clear seasonal trends in atmospheric PAHs. The estimated cancer risk due to inhalation of gas-phase PAHs exceeded the USEPA standard at >90% of the sites.
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#29054623 2017/10/21 Save this To Up
From headwaters to estuary: Distribution and fate of halogenated flame retardants (HFRs) in a river basin near the largest HFR manufacturing base in China.With the gradual phasing out of polybrominated diphenyl ethers (PBDEs), market demands for alternative halogenated flame retardants (HFRs) are increasing. The Laizhou Bay area is the biggest manufacturing base for brominated flame retardants (BFRs) in China, and the Xiaoqing River is the largest and most heavily contaminated river in this region. Water and sediment samples were collected from the headwaters to the estuary of the Xiaoqing River to investigate the distribution and fate of HFRs [i.e., PBDEs, alternative brominated flame retardants (aBFRs) and dechlorane plus (DPs). In the water samples, DPs was the most abundant flame retardant (median: 11.7ng/L), followed by decabromodiphenylethane (DBDPE) (5.92ng/L). In the sediment samples, DBDPE was the predominant flame retardant (39.5ng/g dw), followed by decabromodiphenyl ether (BDE 209) (2.81ng/g dw). The levels of DBDPE exceeded those of BDE 209 in most samples, indicating the overwhelming replacement of BDE 209 by DBDPE in this area. In the river section of this study, point source and atmospheric deposition followed by land runoff were the major factors influencing the distribution of HFRs, whereas in the estuary, riverine discharge, the estuarine maximum turbidity zone (MTZ), and hydrodynamic parameters played more important roles. Manufacturing is a significant source of contamination of the Xiaoqing River basin through atmospheric deposition and wastewater discharge.
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#29054074 2017/10/20 Save this To Up
Temperature dependence of bioelectrochemical CO2 conversion and methane production with a mixed-culture biocathode.This study evaluated the effect of temperature on methane production by CO2 reduction during microbial electrosynthesis (MES) with a mixed-culture biocathode. Reactor performance, in terms of the amount and rate of methane production, current density, and coulombic efficiency, was compared at different temperatures. The microbial properties of the biocathode at each temperature were also analyzed by 16S rRNA gene sequencing. The results showed that the optimum temperature for methane production from CO2 reduction in MES with a mixed-culture cathode was 50°C, with the highest amount and rate of methane production of 2.06±0.13mmol and 0.094±0.01mmolh(-1), respectively. In the mixed-culture biocathode MES, the coulombic efficiency of methane formation was within a range of 19.15±2.31% to 73.94±2.18% due to by-product formation at the cathode, including volatile fatty acids and hydrogen. Microbial analysis demonstrated that temperature had an impact on the diversity of microbial communities in the biofilm that formed on the MES cathode. Specifically, the hydrogenotrophic methanogen Methanobacterium became the predominant archaea for methane production from CO2 reduction, while the abundance of the aceticlastic methanogen Methanosaeta decreased with increased temperature.
1203 related Products with: Temperature dependence of bioelectrochemical CO2 conversion and methane production with a mixed-culture biocathode.ReadiLink™ mFluor™ Vi ReadiLink™ mFluor™ Vi Androgen Receptor (Phosph Androgen Receptor (Phosph ASPARAGINE BROTH Dehydrat Mouse Anti-VZV (mixed epi Rabbit Anti-Human Androge Rabbit Anti-Human Androge Ro 31-8220 methanesulfona Ro 31-8220 methanesulfona Androgen Receptor (Ab 650 Glucose Assay With the La
#29054062 2017/10/20 Save this To Up
The chemostat metabolite spectra of alkaline mixed culture fermentation under mesophilic, thermophilic, and extreme-thermophilic conditions.Alkaline mixed culture fermentation (MCF) is a promising technology for reducing organic waste and producing biochemicals. However, chemostat metabolite spectra that are necessary for constructing a model and analyzing factors are seldom reported. In the present study, the effects of pH on the metabolites distribution in mesophilic (35 °C), thermophilic (55 °C), and extreme-thermophilic (70 °C) alkaline MCF were demonstrated. A chemical oxygen demand balance above 80% was achieved, and the main metabolites included acetate, ethanol, propionate, lactate, and formate. The yields of ethanol and formate increased as pH was increased from 7.5 to higher pH under mesophilic and thermophilic conditions, while the yields of acetate, lactate, and/or propionate decreased. The yields of ethanol, acetate, and formate increased under extreme-thermophilic conditions as pH was increased from 7.5 to 9.0, whereas lactate and hydrogen yields decreased. Low biomass yield under thermophilic and extreme-thermophilic conditions benefited higher metabolite production and minimized the accumulation of sludge.
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#29053606 2017/10/20 Save this To Up
Lung Toxicity of Condensed Aerosol from E-CIG Liquids: Influence of the Flavor and the In Vitro Model Used.The diffusion of e-cigarette (e-CIG) opens a great scientific and regulatory debate about its safety. The huge number of commercialized devices, e-liquids with almost infinite chemical formulations and the growing market demand for a rapid and efficient toxicity screen system that is able to test all of these references and related aerosols. A consensus on the best protocols for the e-CIG safety assessment is still far to be achieved, since the huge number of variables characterizing these products (e.g., flavoring type and concentration, nicotine concentration, type of the device, including the battery and the atomizer). This suggests that more experimental evidences are needed to support the regulatory frameworks. The present study aims to contribute in this field by testing the effects of condensed aerosols (CAs) from three main e-liquid categories (tobacco, mint, and cinnamon as food-related flavor), with (18 mg/mL) or without nicotine. Two in vitro models, represented by a monoculture of human epithelial alveolar cells and a three-dimensional (3D) co-culture of alveolar and lung microvascular endothelial cells were used. Cell viability, pro-inflammatory cytokines release and alveolar-blood barrier (ABB) integrity were investigated as inhalation toxicity endpoints. Results showed that nicotine itself had almost no influence on the modulation of the toxicity response, while flavor composition did have. The cell viability was significantly decreased in monoculture and ABB after exposure to the mints and cinnamon CAs. The barrier integrity was significantly affected in the ABB after exposure to cytotoxic CAs. With the exception of the significant IL-8 release in the monoculture after Cinnamon exposure, no increase of inflammatory cytokines (IL-8 and MCP-1) release was observed. These findings point out that multiple assays with different in vitro models are able to discriminate the acute inhalation toxicity of CAs from liquids with different flavors, providing the companies and regulatory bodies with useful tools for the preliminary screening of marketable products.
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#29052811 2017/10/20 Save this To Up
Four Saccharomyces species differ in their tolerance to various stresses though they have similar basic physiological parameters.Saccharomyces species, which are mostly used in the food and beverage industries, are known to differ in their fermentation efficiency and tolerance of adverse fermentation conditions. However, the basis of their difference has not been fully elucidated, although their genomes have been sequenced and analyzed. Five strains of four Saccharomyces species (S. cerevisiae, S. kudriavzevii, S. bayanus, and S. paradoxus), when grown in parallel in laboratory conditions, exhibit very similar basic physiological parameters such as membrane potential, intracellular pH, and the degree to which they are able to quickly activate their Pma1 H(+)-ATPase upon glucose addition. On the other hand, they differ in their ability to proliferate in media with a very low concentration of potassium, in their osmotolerance and tolerance to toxic cations and cationic drugs in a growth-medium specific manner, and in their capacity to survive anhydrobiosis. Overall, S. cerevisiae (T73 more than FL100) and S. paradoxus are the most robust, and S. kudriavzevii the most sensitive species. Our results suggest that the difference in stress survival is based on their ability to quickly accommodate their cell size and metabolism to changing environmental conditions and to adjust their portfolio of available detoxifying transporters.
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