Search results for: Albumin antiswine
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Serologic studies of experimentally induced Salmonella choleraesuis var kunzendorf infection in pigs.
Two indirect ELISA containing outer membrane protein (OMP) and lipopolysaccharide (LPS) antigens from a field isolate of Salmonella choleraesuis var kunzendorf were developed and evaluated in experimentally infected and uninfected control pigs. Experimentally induced infection with S choleraesuis was successfully established in 10 pigs by oral inoculation with 10(8) organisms, and 3 pigs died of clinical salmonellosis at postinoculation (PI) weeks 1, 2, and 4. Swab specimens from tonsils, nostrils, and rectum of pigs were obtained for culture, and sera were evaluated at weekly intervals for 9 weeks after inoculation. The ELISA containing OMP and LPS antigens with either anti-swine IgG or protein albumin-to-globulin ratio (antiglobulin) conjugates were standardized for serologic evaluation. All 4 ELISA (2 OMP and 2 LPS) detected seroconversion by PI week 3 and had sensitivities and specificities of 97.8 and 88.8, 100 and 100, 95.6 and 88.8, and 93.3 and 72.5%, at their ideal cutoff points (negative mean optical density +2 SD). There was excellent agreement between all 4 ELISA systems as determined by kappa values. Cultures of fecal, tonsil, and nasal swab specimens were positive for S choleraesuis until the fourth week of infection. Fecal swab specimens from 1 pig were positive for S choleraesuis until PI week 7. Persistent infection after antemortem culture results were negative was detected by all 4 ELISA, which indicated consistently high titers until the end of PI week 9.(ABSTRACT TRUNCATED AT 250 WORDS)S Srinand, R A Robinson, J E Collins, K V Nagaraja
2840 related Products with: Serologic studies of experimentally induced Salmonella choleraesuis var kunzendorf infection in pigs.
96T50 ul1 mg 100ul100ug5ug2ug100ug100 ul900 tests400 ug
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#1751224 //
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Prolongation of cardiac xenograft function after reduction of natural antibodies using double filtration plasmapheresis.
A double filtration plasmapheresis (DFPP) technique was applied to selectively remove the natural antibodies in discordant xenotransplantation. A swine heart was heterotopically transplanted in a canine neck after two DFPP procedures during which 200 to 500 ml of plasma were replaced with the same amount of Hartman's solution containing 7% human albumin. Mean removal rate of IgC and IgM by DFPP ranged from 74.2 +/- 0.5 to 95.9 +/- 2.8%. The anti-swine lymphocytotoxic reaction of canine serum was decreased after DFPP but still remained in low titers. Mean graft survival time in the group treated by DFPP in which 1000 ml of plasma was totally replaced was prolonged to 107 +/- 47 min, while it was 9 +/- 5 min in the group without any treatment (p less than 0.01). Deposits of canine IgM and C3 on the vascular endothelium of the graft were observed on immunofluorescence, while there were no deposits of IgG. In conclusion, DFPP effectively removed the natural antibodies, resulting in prolongation of xenograft survival time. Immunofluorescence study suggested that natural IgM antibodies played an important role in this xeno-hyperacute rejection.H Suga, H Ishida, M Kimikawa, Y Hayasaka, S Teraoka, T Agishi, K Ota
2071 related Products with: Prolongation of cardiac xenograft function after reduction of natural antibodies using double filtration plasmapheresis.
1 mg200.00 ug0.1 mg50.00 ug200.00 ug200.00 ug1 mg1 mg1 mg0.2 mg100.00 ug200.00 ug
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#2462304 //
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Characterization of isolated porcine intestinal mucosal mast cells following infection with Ascaris suum.
Porcine intestinal mucosal mast cells (IMMC) were isolated from intestinal tissues of swine by enzymatic digestion and density gradient separation. Helminth-free swine and swine exposed to the nematode parasite, Ascaris suum, were used as a source of intestinal tissue. Up to 40% of the isolated intestinal cells stained metachromatically with toluidine blue pH 3.0, indicating the presence of IMMC. The histamine content of this cell population ranged from 2.9-8.9 pg per toluidine blue-positive IMMC, regardless of the animal source. Enrichment procedures that increased the proportion of toluidine blue-positive IMMC from the isolated intestinal cell population correlated with an increase in the amount of histamine detected in the cell population, indicating that toluidine blue-positive IMMC were the major source of histamine in this heterogeneous cell population. However, only cells isolated from the intestines of parasite-exposed swine released histamine in vitro after mixing with antigens derived from A. suum. Cells from the intestines of both helminth-free and parasite-exposed swine did not release histamine after mixing with a non-parasite hapten-protein molecule DNP-human serum albumin, but did release greater than 90% of their total histamine after lysis with Triton X-100 or with the Ca2+ ionophore (A23187). The stimulus for acquired responsiveness of IMMC to A. suum antigens in vitro was parasitic infection in vivo because helminth-free swine maintained in confinement on concrete yielded IMMC that specifically released histamine in the presence of parasite antigens only after 3 weeks of daily experimental inoculations with A. suum eggs. IMMC isolated from the entire length of the small intestines of infected pigs were responsive to antigens in vitro, but the relative number of IMMC isolated and their level of histamine release decreased from the anterior to the posterior end. IMMC isolated from infected swine were also stimulated to release histamine in vitro by viable second stage larvae of A. suum and by treatment with anti-swine immunoglobulin. Responsiveness to both parasite antigens and anti-immunoglobulin were totally eliminated, however, by a brief treatment of the cells with acidic buffer, suggesting that an acid-dissociable cell-bound antibody molecule was responsible for specific antigen-induced histamine release by IMMC.M Ashraf, J F Urban, T D Lee, C M Lee
2500 related Products with: Characterization of isolated porcine intestinal mucosal mast cells following infection with Ascaris suum.
2 mL100ml30ml96 wells15ml96 assays500UOne 96-Well Strip Micropl100reactions 502.00 flask
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Immunization of Swine for Production of Antibody Against Zearalenone.
Production of antisera specific for zearalenone was investigated in swine for potential use in prophylaxis against zearalenone hyperestrogenism. Swine were immunized with zearalenone-6'-carboxymethyloxime bovine serum albumin conjugate by four different protocols. For detection of antizearalenone antibody, a simple indirect enzyme-linked immunosorbent assay (ELISA) was devised whereby porcine antiserum was incubated over a zearalenone-6'-carboxymethyloxime poly-L-lysine solid phase and total bound antibodies were detected with peroxidase-labeled anti-swine serum. The optimal immunization protocol consisted of an initial injection of 5 mg of conjugate followed by a 2-mg boost at 4 wk and was sufficient to obtain anti-zearalenone titers of 1:5120 in 8 wk. Competitive indirect ELISA for zearalenone using this antiserum had an assay detection limit of 10 ng/ml for the toxin. Cross-reactivity of the antiserum with α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol were 33, 25, 6, and 10%, respectively.J J Pestka, M-T Liu, B K Knudson, M G Hogberg