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#24131426   2014/02/24 To Up

Development of an immuno-based colorimetric assay for white spot syndrome virus.

White spot syndrome virus (WSSV) is a major cause of infectious disease in cultured shrimp. A fast and reliable method for detecting and monitoring the amount of WSSV during farming would be extremely useful. This work describes a sandwich immunoassay that uses anti-GST-VP26, a WSSV-binding protein (WBP), and modified streptavidin magnesphere paramagnetic particles (SMPPs) to develop the technique. The WBP was immobilized on SMPPs and later bound to different copies of WSSV. The binding was detected using anti-GST-VP26 conjugated to alkaline phosphatase. This enzymatic reaction successfully changed the test solution to a concentration-dependent yellow color that was measured at 405 nm. The sensitivity of this method was between 1.6 × 10(4) and 1.6 × 10(7) copies µL(-1) of WSSV. In this study, the color for detection and semiquantitative analysis is easily observed and measured and can lead to the development of a test kit for screening WSSV during shrimp farming.
Suchera Loyprasert-Thananimit, Akrapon Saleedang, Panchalika Deachamag, Thanyaporn Waiyapoka, Maitee Neulplub, Wilaiwan Chotigeat

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96 Tests50 assays100tests0.25 mg0.1ml (1mg/ml)100ug Lyophilized96 Tests/kit1 kit(96 Wells)

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