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Search results for: Amplite™ Colorimetric Peroxidase Assay Kit *Blue Color*

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#38331555   2024/01/22 To Up

Multienzyme cascades analysis of α-glucosidase by oxygen deficient MoO.

Recently, the enzymatic cascade reactions during the cellular process are widely used for fabricating robust biosensors and they have attracted extensive attention in analyzing various clinical biomarkers. The enzymatic cascades analysis is commonly based on the peroxidase (POD)/oxidase coupled system. However, the requirement of harsh acidic environment, poor stability and interference from the oxidase further limit their analytical practicability. Herein, novel chromogenic nanomaterials with HO sensitive features are urgently required to replace the POD nanozyme in enzymatic cascades based bioanalysis.
Fengxian Zhang, Jiawei Liu, Zhi Chen, Erjing Wang, Cao Li, Jiaji Cheng, Jie Shen, Ziqiang Xu

1351 related Products with: Multienzyme cascades analysis of α-glucosidase by oxygen deficient MoO.

1 module1 module 15 ml 50 ug1 module1 module1 module1 module1 module 1 kit(s)

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#35424527   2021/12/22 To Up

Peroxidase catalytic activity of carbon nanoparticles for glutathione detection.

Peroxidases are present widely in microorganisms and plants, and catalyze many reactions. However, the activity of natural peroxidases is susceptible to external conditions. We prepared carbon nanoparticles (CNPs) using an environmentally friendly and simple method. These CNPs were demonstrated to possess intrinsic peroxidase-like activity. CNPs could catalyze the reaction of a peroxidase substrate, 3,3,5,5-tetramethylbenzidine (TMB), in the presence of HO to produce a blue solution at 652 nm. CNPs exhibited higher peroxidase activity than that of other carbon-based nanomaterials. Moreover, CNPs retained their high peroxidase activity after being reused several times. Glutathione (GSH) can change the blue color of oxidized TMB into a colorless hue at 652 nm. Based on this fact, qualitative and quantitative approaches were employed to detect GSH using a colorimetric method. This method showed a broad detection range (2.5-50 μM) with a limit of detection of 0.26 μM. This method was shown to be accurate for GSH detection in a cell culture medium compared with that using a commercial assay kit. Our findings could facilitate application of CNPs in biomedical areas.
Lijuan Chen, Xiang Li, Zezhi Li, Kejian Liu, Jianping Xie

1351 related Products with: Peroxidase catalytic activity of carbon nanoparticles for glutathione detection.

100 assays100 ul200 Cuvette480 tests100 ul4x96 well plate0.5 mg100 assays

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#31280384   2019/07/06 To Up

Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic.

A visualization strategy is described for the detection of clenbuterol (CLB). It is using of antibody against dsDNA and G-quadruplex/hemin labeled on a metal organic framework of type MIL-101(Fe) (G-quadruplex/hemin-anti-DNA/MIL-101) acting as a peroxidase mimetic, and magnetic beads modified with aptamer and complementary DNA (MB/Apt-cDNA) as capture probes. The detection reagent was prepared via the reactions between the double stranded DNA (Apt-cDNA) in capture probes and anti-DNA in peroxidase mimetic. In the presence of CLB, the aptamer on the magnetic beads preferentially binds CLB, and the peroxidase mimetic is released to the supernatant after magnetic separation. The released peroxidase mimetic can catalyze the TMB/HO chromogenic system under mild conditions. This leads to the development of a blue-green coloration whose absorbance is measured at 650 nm. The detection limit is as low as 34 fM of CLB. The method was applied to the determination of CLB in pork samples and gave results that were consistent with data obtained with an ELISA kit. Graphical abstract A visualization strategy is described for the detection of clenbuterol. The selectivity of detection system for clenbuterol is excellent compared with other interferents. The method was applied to the determination of CLB in pork samples.
Yuandong Zhang, Hong-Xia Ren, Yang-Bao Miao

1290 related Products with: Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic.

100ug1000 tests50 ug 200ug100ug100ul1,000 tests50 ug 100ug100ul100ug50 ug

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#27611475   2016/08/30 To Up

Size-controlled preparation of peroxidase-like graphene-gold nanoparticle hybrids for the visible detection of norovirus-like particles.

A hybrid structure of graphene-gold nanoparticles (Grp-Au NPs) was designed as a new nanoprobe for colorimetric immunoassays. This hybrid structure was prepared using chloroauric acid, sodium formate and Grp flakes at room temperature. Au NPs attached strongly onto the Grp surface, and their size was controlled by varying the sodium formate concentration. The Raman intensity of the Grp-Au NP hybrids was significantly enhanced at 1567cm and 2730cm compared with those of pristine Grp because of the electronic interaction between Au NPs and Grp. The Grp-Au NPs with a hybrid structure catalyzed the oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) with HO, developing a blue color in aqueous solution. This catalytic activity was utilized to detect norovirus-like particles (NoV-LPs) in human serum. The enhanced colorimetric response was monitored using Ab-conjugated-Grp-Au NPs and found to depend on the NoV-LP concentration, exhibiting a linear response from 100pg/mL to 10μg/mL. The limit of detection (LOD) of this proposed method was 92.7pg/mL, 112 times lower than that of a conventional enzyme-linked immunosorbent assay (ELISA). The sensitivity of this test was also 41 times greater than that of a commercial diagnostic kit. The selectivity of the Grp-Au NPs was tested with other viruses, and no color changes were observed. Therefore, the proposed system will facilitate the utilization of the intrinsic peroxidase-like activity of Grp-Au NPs in medical diagnostics. We believe that the engineered catalytic Grp-Au NP hybrids could find potential applications in the future development of biocatalysts and bioassays.
Syed Rahin Ahmed, Kenshin Takemeura, Tian-Cheng Li, Noritoshi Kitamoto, Tomoyuki Tanaka, Tetsuro Suzuki, Enoch Y Park

2291 related Products with: Size-controlled preparation of peroxidase-like graphene-gold nanoparticle hybrids for the visible detection of norovirus-like particles.

100tests250tests100tests100tests100tests100tests100tests100tests2x96 well plate100tests

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#25032681   2014/07/07 To Up

Visual detection of blood glucose based on peroxidase-like activity of WS2 nanosheets.

Tungsten disulfide (WS2) nanosheets were discovered to possess intrinsic peroxidase-like activity and catalyze the peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) to produce a color reaction in the presence of H2O2. Based on this finding, a colorimetric method and a portable test kit for the visual detection of blood glucose have been developed by using glucose oxidase (GOx) and WS2 nanosheets-catalyzed reactions. The linear range for glucose was ranged from 5 to 300 μM (R(2)=0.999) with the detection limit of 2.9 μM. The portable test kit was successfully evaluated glucose levels in serum samples from normal persons and diabetes persons by the observable color change from pale yellow to yellow-green, blue-green.
Tianran Lin, Liangshuang Zhong, Zhiping Song, Liangqia Guo, Hanyin Wu, Qingquan Guo, Ying Chen, FengFu Fu, Guonan Chen

2456 related Products with: Visual detection of blood glucose based on peroxidase-like activity of WS2 nanosheets.

2x96 well plate100 assays100tests 5 G100 assays100tests100 assays

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