Only in Titles

Search results for: Androstane-3a,17b-diol Glucuronide(5a) Antibody

paperclip

#38499459   2024/03/18 To Up

Plasma exchange for the management of digoxin toxicity in an individual with an acute kidney injury: A case report.

Digoxin toxicity can be life-threatening. Digoxin-specific antibody (DSA) fragments are used in severe digoxin toxicity, binding to serum-free digoxin and enabling increased renal excretion. In severe renal impairment, clearance of these complexes is prolonged, leading to rebound toxicity. Digoxin and DSA complexes are not dialysable. We present a case of a gentleman with severe digoxin toxicity and acute kidney injury (AKI). Despite receiving DSA doses, his digoxin levels rebounded and symptoms persisted. Based on published case reports, plasma exchange (PEX) after further dosing was arranged. PEX facilitated the removal of digoxin-DSA complexes, bypassing renal excretion. During PEX, clinical signs improved and were sustained. He did not require further dialysis or PEX, renal function recovered and he was discharged. This case highlights challenges in the management of severe digoxin toxicity in patients with a concurrent AKI. The use of PEX enabled digoxin-DSA complex removal and should be considered in these circumstances.
Hannah Preston, Emma Cannon, Simon Watson

1866 related Products with: Plasma exchange for the management of digoxin toxicity in an individual with an acute kidney injury: A case report.



Related Pathways

paperclip

#38499458   2024/03/17 To Up

COVID-19 vaccination in cancer patients: Immune responses one year after the third dose.

Cancer patients (CPs), being immunosuppressed due to the treatment received or to the disease itself, are more susceptible to infections and their potential complications, showing therefore an increased risk of developing severe COVID-19 compared to the general population. We evaluated the immune responses to anti-SARS-CoV-2 vaccination in patients with solid tumors one year after the administration of the third dose and the effect of cancer treatment on vaccine immunogenicity was assessed. Healthy donors (HDs) were enrolled. Binding and neutralizing antibody (Ab) titers were evaluated using chemiluminescence immunoassay (CLIA) and Plaque Reduction Neutralization Test (PRNT) respectively. T-cell response was analyzed using multiparametric flow cytometry. CPs who were administered three vaccine doses showed lower Ab titers than CPs with four doses and HDs. Overall, a lower cell-mediated response was found in CPs, with a predominance of monofunctional T-cells producing TNFα. Lower Ab titers and a weaker T-cell response were observed in CPs without prior SARS-CoV-2 infection when compared to those with a previous infection. While no differences in the humoral response were found comparing immunotherapy and non-immunotherapy patients, a stronger T-cell response in CPs treated with immunotherapy was observed. Our results emphasize the need of booster doses in cancer patients to achieve a level of protection similar to that observed in healthy donors and underlines the importance of considering the treatment received to reach a proper immune response.
Roberta Campagna, Federica Dominelli, Maria Antonella Zingaropoli, Fabio Ciurluini, Giorgia Grilli, Alessandra Amoroso, Angelo De Domenico, Donatella Amatore, Maria Stella Lia, Enrico Cortesi, Vincenzo Picone, Claudio Maria Mastroianni, Maria Rosa Ciardi, Riccardo De Santis, Florigio Lista, Guido Antonelli, Ombretta Turriziani

1063 related Products with: COVID-19 vaccination in cancer patients: Immune responses one year after the third dose.



Related Pathways

paperclip

#38499390   2024/03/18 To Up

Site-Specific Conjugation of Native Antibody: Transglutaminase-Mediated Modification of a Conserved Glutamine While Maintaining the Primary Sequence and Core Fc Glycan via Trimming with an Endoglycosidase.

A versatile chemo-enzymatic tool to site-specifically modify native (nonengineered) antibodies is using transglutaminase (TGase, E.C. 2.3.2.13). With various amines as cosubstrates, this enzyme converts the unsubstituted side chain amide of glutamine (Gln or Q) in peptides and proteins into substituted amides (i.e., conjugates). A pleasant surprise is that only a single conserved glutamine (Gln295) in the Fc region of IgG is modified by microbial TGase (mTGase, EC 2.3.2.13), thereby providing a highly specific and generally applicable conjugation method. However, prior to the transamidation (access to the glutamine residue by mTGase), the steric hindrance from the nearby conserved N-glycan (Asn297 in IgG1) must be reduced. In previous approaches, amidase (PNGase F, EC 3.5.1.52) was used to completely remove the N-glycan. However, PNGase F also converts a net neutral asparagine (Asn297) to a negatively charged aspartic acid (Asp297). This charge alteration may markedly change the structure, function, and immunogenicity of an IgG antibody. In contrast, in our new method presented herein, the N-glycan is trimmed by an endoglycosidase (EndoS2, EC 3.2.1.96), hence retaining both the core N-acetylglucosamine (GlcNAc) moiety and the neutral asparaginyl amide. The trimmed glycan also reduces or abolishes Fc receptor-mediated functions, which results in better imaging agents by decreasing nonspecific binding to other cells (e.g., immune cells). Moreover, the remaining core glycan allows further derivatization such as glycan remodeling and dual conjugation. Practical and robust, our method generates conjugates in near quantitative yields, and both enzymes are commercially available.
Amissi Sadiki, Shanshan Liu, Shefali R Vaidya, Eric M Kercher, Ryan T Lang, James McIsaac, Bryan Q Spring, Jared R Auclair, Zhaohui Sunny Zhou

2172 related Products with: Site-Specific Conjugation of Native Antibody: Transglutaminase-Mediated Modification of a Conserved Glutamine While Maintaining the Primary Sequence and Core Fc Glycan via Trimming with an Endoglycosidase.

100ug 1 ea.

Related Pathways

paperclip

#38499356   2024/03/18 To Up

Concentrations of subcutaneously administered belimumab in human breast milk of a woman with systemic lupus erythematosus: a case report.


Birgit S Blomjous, Marjon A de Boer, Mirjam M van Weissenbruch, Koen C J Laan, Theo Rispens, Alexandre E Voskuyl, Irene E M Bultink

2289 related Products with: Concentrations of subcutaneously administered belimumab in human breast milk of a woman with systemic lupus erythematosus: a case report.



Related Pathways

    No related Items
paperclip

#38499233   2024/03/16 To Up

Epitope mapping of SARS-CoV-2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls.

Understanding protein-protein interactions is crucial for drug design and investigating biological processes. Various techniques, such as CryoEM, X-ray spectroscopy, linear epitope mapping, and mass spectrometry-based methods, can be employed to map binding regions on proteins. Commonly used mass spectrometry-based techniques are cross-linking and hydrogen‑deuterium exchange (HDX). Another approach, hydroxyl radical protein footprinting (HRPF), identifies binding residues on proteins but faces challenges due to high initial costs and complex setups. This study introduces a generally applicable method using Fenton chemistry for epitope mapping in a standard mass spectrometry laboratory. It emphasizes the importance of controls, particularly the inclusion of a negative antibody control, not widely utilized in HRPF epitope mapping. Quantification by TMT labelling is introduced to reduce false positives, enabling direct comparison between sample conditions and biological triplicates. Additionally, six technical replicates were incorporated to enhance the depth of analysis. Observations on the receptor-binding domain (RBD) of SARS-CoV-2 Spike Protein, Alpha and Delta variants, revealed both binding and opening regions. Significantly changed peptides upon mixing with a negative control antibody suggested structural alterations or nonspecific binding induced by the antibody alone. Integration of negative control antibody experiments and high overlap between biological triplicates led to the exclusion of 40% of significantly changed regions. The final identified binding region correlated with existing literature on neutralizing antibodies against RBD. The presented method offers a straightforward implementation for HRPF analysis in a generic mass spectrometry-based laboratory. Enhanced data reliability was achieved through increased technical and biological replicates alongside negative antibody controls.
Daniel Nyberg Larsen, Jakub Zbigniew Kaczmarek, Yaseelan Palarasah, Jonas Heilskov Graversen, Peter Højrup

2728 related Products with: Epitope mapping of SARS-CoV-2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls.

200ul100ug Lyophilized250ul200ul100ug Lyophilized200ul100ug Lyophilized100ug Lyophilized200ul100ug Lyophilized20ug100ug Lyophilized

Related Pathways

paperclip

#38499218   2024/03/16 To Up

Inducible IL-2 production and IL-2 cell expansion are landmark events for T-cell activation of teleost.

As a multipotent cytokine, interleukin (IL)-2 plays important roles in activation, differentiation and survival of the lymphocytes. Although biological characteristics and function of IL-2 have been clarified in several teleost species, evidence regarding IL-2 production at the cellular and protein levels is still scarce in fish due to the lack of reliable antibody. In this study, we developed a mouse anti-Nile tilapia IL-2 monoclonal antibody (mAb), which could specifically recognize IL-2 protein and identify IL-2-producing lymphocytes of tilapia. Using this mAb, we found that CD3 T cells, but not CD3 lymphocytes, are the main cellular source of IL-2 in tilapia. Under resting condition, both CD3CD4-1 T cells and CD3CD4-1 T cells of tilapia produce IL-2. Moreover, the IL-2 protein level and the frequency of IL-2 T cells significantly increased once T cells were activated by phytohemagglutinin (PHA) or CD3 plus CD28 mAbs in vitro. In addition, Edwardsiella piscicida infection also induces the IL-2 production and the expansion of IL-2 T cells in the spleen lymphocytes. These findings demonstrate that IL-2 takes part in the T-cell activation and anti-bacterial adaptive immune response of tilapia, and can serve as an important marker for T-cell activation of teleost fish. Our study has enriched the knowledge regarding T-cell response in fish species, and also provide novel perspective for understanding the evolution of adaptive immune system.
Jiansong Zhang, Kang Li, Yi Cao, Ding Wang, Jie Cheng, Haiyou Gao, Ming Geng, Jialong Yang, Xiumei Wei

1454 related Products with: Inducible IL-2 production and IL-2 cell expansion are landmark events for T-cell activation of teleost.

500 ml25ml25ml2500 assays5 x 200ul/Unit25ml2500 assays

Related Pathways

paperclip

#38499217   2024/03/16 To Up

Construction and analysis of the immune effect of two different vaccine types based on Vibrio harveyi VgrG.

Vibrio harveyi poses a significant threat to fish and invertebrates in mariculture, resulting in substantial financial repercussions for the aquaculture sector. Valine-glycine repeat protein G (VgrG) is essential for the type VI secretion system's (T6SS) assembly and secretion. VgrG from V. harveyi QT520 was cloned and analyzed in this study. The localization of VgrG was determined by Western blot, which revealed that it was located in the cytoplasm, secreted extracellularly, and attached to the membrane. The effectiveness of two vaccinations against V. harveyi infection-a subunit vaccine (rVgrG) and a DNA vaccine (pCNVgrG) prepared with VgrG was evaluated. The findings indicated that both vaccines provided a degree of protection against V. harveyi challenge. At 4 weeks post-vaccination (p.v.), the rVgrG and pCNVgrG exhibited relative percent survival rates (RPS) of 71.43% and 76.19%, respectively. At 8 weeks p.v., the RPS for rVgrG and pCNVgrG were 68.21% and 72.71%, respectively. While both rVgrG and pCNVgrG elicited serum antibody production, the subunit vaccinated fish demonstrated significantly higher levels of serum anti-VgrG specific antibodies than the DNA vaccine group. The result of qRT-PCR demonstrated that the expression of major histocompatibility complex (MHC) class Iα, tumor necrosis factor-alpha (TNF-α), interferon γ (IFNγ), and cluster of differentiation 4 (CD4) were up-regulated by both rVgrG and pCNVgrG. Fish vaccinated with rVgrG and pCNVgrG exhibited increased activity of acid phosphatase, alkaline phosphatase, superoxide dismutase, and lysozyme. These findings suggest that VgrG from V. harveyi holds potential for application in vaccination.
Xiangyu Du, Minjie Kang, Chunhuan Yang, Xinping Yao, Lvliang Zheng, Ying Wu, Panpan Zhang, Han Zhang, Yongcan Zhou, Yun Sun

2146 related Products with: Construction and analysis of the immune effect of two different vaccine types based on Vibrio harveyi VgrG.

5 G100ug100ug LyophilizedOne 96-Well Microplate Ki1 module 15 ml 1 module500 MG0.2 mg2000 IU1 module

Related Pathways

paperclip

#38499192   2024/03/18 To Up

Glanzmann Thrombasthenia 10 Years Later: Progress Made and Future Directions.

Glanzmann thrombasthenia (GT) is the most common inherited platelet disorder (IPD) with mucocutaneous bleeding and a failure of platelets to aggregate when stimulated. The molecular cause is insufficient or defective αIIbβ3, an integrin encoded by the and genes. On activation αIIbβ3 undergoes conformational changes and binds fibrinogen (Fg) and other proteins to join platelets in the aggregate. The application of next-generation sequencing (NGS) to patients with IPDs has accelerated genotyping for GT; progress accompanied by improved mutation curation. The evaluation by NGS of variants in other hemostasis and vascular genes is a major step toward understanding why bleeding varies so much between patients. The recently discovered role for glycoprotein VI in thrombus formation, through its binding to fibrin and surface-bound Fg, may offer a mechanosensitive back-up for αIIbβ3, especially at sites of inflammation. The setting up of national networks for IPDs and GT is improving patient care. Hematopoietic stem cell therapy provides a long-term cure for severe cases; however, prophylaxis by monoclonal antibodies designed to accelerate fibrin formation at injured sites in the vasculature is a promising development. Gene therapy using lentil-virus vectors remains a future option with CRISPR/Cas9 technologies offering a promising alternative route.
Alan T Nurden, Paquita Nurden

2874 related Products with: Glanzmann Thrombasthenia 10 Years Later: Progress Made and Future Directions.

1000 TESTS/0.65ml100ug100ug100ul100ug100ug100ug100ul100ug100ug1000 tests100ul

Related Pathways

paperclip

#38499168   2024/03/16 To Up

Formation and clinical effects of anti-drug antibodies against biologics in psoriasis treatment: An analysis of current evidence.

Formation of anti-drug antibodies (ADAs) against biologics is an important cause of psoriasis treatment failure.
Xiaoying Sun, Ziyang Cui, Qingyun Wang, Liu Liu, Xiaojie Ding, Jiao Wang, Xiaoce Cai, Bin Li, Xin Li

1193 related Products with: Formation and clinical effects of anti-drug antibodies against biologics in psoriasis treatment: An analysis of current evidence.

100 μg50100 μg100 μg200 100 μg100 μg100 μg100 μg100 μg100 μg

Related Pathways

paperclip

#38499123   2024/03/16 To Up

Rapid and sensitive detection of SARS-CoV-2 IgM through luciferase luminescence on an automatic platform.

S
Yibing Zhang, Yun Zhang, Wenhao Zhou, Ping He, Xueni Sun, Junhua Li, Hongping Wei, Junping Yu

1482 related Products with: Rapid and sensitive detection of SARS-CoV-2 IgM through luciferase luminescence on an automatic platform.

200ul10 mg200ul200ug200ug100 ul25ml 100ug Lyophilized200ul100ug Lyophilized25 mg200ul

Related Pathways

    No related Items