Search results for: Anti Galectin(Gal-3) Human, Polyclonal Antibody, Rabbit Antibodies
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A practical strategy for immunofluorescent detecting multiple targets in mouse tissues without restrictions on the host specious resources of the primary antibodies.Commercial deficiency of practical system to label multiple targets in experimental mouse tissues significantly hinders the feasibility to study the potential association between/among multiple targets using tissue-based immunofluorescence (IF) staining. We have developed a new protocol to do dual - labeling immunofluorescences on mouse tissues by combining direct and indirect immunofluorescence, making it possible to use commercial antibodies from the same specious (rabbit) to detect multiple targets in formalin-fixed paraffin-embedded (FFPE) archival mouse tissues simultaneously. This method applies indirect immunofluorescence to assess the first antigen in mouse tissues by using a rabbit anti-mouse polyclonal antibody and goat anti-rabbit antibody. After that, normal rabbit serum was employed to blocking the free binding sites of the previous antibodies. Direct immunofluorescence was used to assess the second antigen by a commercial kit-labeled rabbit anti-human (mouse) antibody at different emission wavelength. At last, cell nuclei were co-stained by DAPI. The outcomes demonstrated that this protocol obtain promising signals of both antigens and the nuclei. Moreover, this method also works on infection disease models in which samples are often over fixed due to biosafety rules.
1834 related Products with: A practical strategy for immunofluorescent detecting multiple targets in mouse tissues without restrictions on the host specious resources of the primary antibodies.Multiple organ tumor tiss HIV1 integrase antibody, Normal mouse multiple org Multiple organ cancer tis Mouse Anti-Bacteroides th MOUSE ANTI BOVINE ROTAVIR CD8 antibody, Monoclonal HIV1 gp41 antibody, Monoc HIV2 p26 antibody, Monocl Measles Virus Nucleoprote Measles Virus nucleoprote HIV1 Nef antibody, Monocl
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A mouse model of seizures in anti-N-methyl-d-aspartate receptor encephalitis.Seizures develop in 80% of patients with anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis, and these represent a major cause of morbidity and mortality. Anti-NMDAR antibodies have been linked to memory loss in encephalitis; however, their role in seizures has not been established. We determined whether anti-NMDAR antibodies from autoimmune encephalitis patients are pathogenic for seizures.
1723 related Products with: A mouse model of seizures in anti-N-methyl-d-aspartate receptor encephalitis.Mouse Anti-Human Interleu Goat Anti-Human, Mouse Ca Goat Anti-Rat CCKA Recept Anti AGO2 Mouse, Monoclon Anti AGO2 Mouse, Monoclon Goat Anti-Mouse SAR1, (in Goat Anti-Mouse Rab17 (mo Goat Anti-Mouse IA2, (int Goat Anti-Human, Mouse HI Goat Anti-Human FTO (Mous Goat Anti-Human, Mouse EB Goat Anti-Mouse, Rat DLL1
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Quantitative and qualitative changes in anti-Neu5Gc antibody response following rabbit anti-thymocyte IgG induction in kidney allograft recipients.Antibodies of non-human mammals are glycosylated with carbohydrate antigens, such as galactose-α-1-3-galactose (α-Gal) and N-glycolylneuraminic acid (Neu5Gc). These non-human carbohydrate antigens are highly immunogenic in humans due to loss-of-function mutations of the key genes involved in their synthesis. Such immunogenic carbohydrates are expressed on therapeutic polyclonal rabbit anti-human T-cell IgGs (anti-thymocyte globulin; ATG), the most popular induction treatment in allograft recipients. To decipher the quantitative and qualitative response against these antigens in immunosuppressed patients, particularly against Neu5Gc, which may induce endothelial inflammation in both the graft and the host. We report a prospective study of the antibody response against α-Gal and Neu5Gc-containing glycans following rabbit ATG induction compared to controls. We show a drop in the overall levels of anti-Neu5Gc antibodies at 6 and 12 months post-graft compared to the pre-existing levels due to the major early immunosuppression. However, in contrast, in a cross-sectional study there was a highly significant increase in anti-Neu5Gc IgGs levels at 6 months post-graft in the ATG-treated compared to non-treated patients(P = 0.007), with a clear hierarchy favouring anti-Neu5Gc over anti-Gal response. A sialoglycan microarray analysis revealed that the increased anti-Neu5Gc IgG response was still highly diverse against multiple different Neu5Gc-containing glycans. Furthermore, some of the ATG-treated patients developed a shift in their anti-Neu5Gc IgG repertoire compared with the baseline, recognizing different patterns of Neu5Gc-glycans. In contrast to Gal, Neu5Gc epitopes remain antigenic in severely immunosuppressed patients, who also develop an anti-Neu5Gc repertoire shift. The clinical implications of these observations are discussed.
1629 related Products with: Quantitative and qualitative changes in anti-Neu5Gc antibody response following rabbit anti-thymocyte IgG induction in kidney allograft recipients.Anti beta3 AR Human, Poly Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-NOS-2 iNOS Po
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Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres.A new electrochemical immunosensing protocol by coupling with a magneto-controlled flow-through microfluidic device was developed for the sensitive detection of alpha-fetoprotein (AFP) on magnetic beads (MB) using ferrocene derivative polymer nanospheres (FDNP) as the electroactive mediators. The immunosensing probe was prepared by covalent conjugation of monoclonal mouse anti-human AFP antibodies with magnetic beads, while the recognition element was constructed by means of immobilizing polyclonal rabbit anti-human AFP antibodies on the redox FDNP. Upon target AFP introduction, the sandwich-type immunoreaction was carried out between the immunosensing probe and the recognition element, and the formed immunocomplex was captured in the detection cell with an external magnet. Ferrocene polymer nanospheres synthesized by infinite coordination polymerization were utilized as the signal-generation tags during the electrochemical measurement. Under optimal conditions, the magneto-controlled flow-through immunosensing platform exhibited good electrochemical responses toward target AFP within a dynamic working range of 0.01-100 ng mL-1 and with a low detection limit of 5.7 pg mL-1. The nanoparticles-based sensing systems also gave good reproducibility, high specificity and long-term stability. Moreover, our strategy displayed well-matched accuracy for the analysis of human serum specimens relative to commercial Roche 2010 Electrochemiluminescence (ECL) Automated Analyzer.
1272 related Products with: Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres.Rabbit Anti-ZHX2 Alpha fe Rabbit Anti-ZHX2 Alpha fe Glucose Assay With the La Protein A Magnetic Beads Protein G Magnetic Beads Protein A G Magnetic Bead Protein L Magnetic Beads Protein A G L Magnetic Be Homogenizer for 24 sample Homogenizer for 8 samples Rabbit Anti-14-3-3(Alpha Rabbit Anti-AGPA Alpha 1
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Production and Characterization of Monoclonal and Polyclonal Antibodies Against Truncated Recombinant Dickkopf-1 as a Candidate Biomarker.Several studies have reported an increased serum level of Dickkopf (DKK-1) protein in a variety of cancers, including multiple myeloma, lung, colorectal, bone loss, and Alzheimer's disease. This protein has potential to be used as a biomarker for the diagnosis of some cancers, especially bone loss in multiple myeloma. In the present study, to measure the concentration level of DKK-1 protein, rabbit polyclonal antibody (pAb) and mouse monoclonal antibodies (mAbs) were produced against this protein. New Zealand white rabbits and BALB/c mice were immunized with the chimeric recombinant DKK-1 antigen. Immunized mouse spleen cells were fused with SP2/0 cells to generate anti-rDKK-1 antibody-producing hybridoma cells. Antibodies were purified by protein A affinity chromatography and assessed using sodium dodecyl sulfate polyacrylamide gel, western blotting and enzyme-linked immunosorbent assay. These results implied that the pAb and mAb were produced against the DKK-1 protein. The Kd value of 5 × 10 M was recorded for the mAb MR6F3 toward native DKK-1, and the Ig isotype was identified as IgG2b. No cross-reactivity was shown with DKK-2 by MR6F3. Collectively, our results revealed that the produced pAb and mAb could be used in the measurement of DKK-1 protein.
2007 related Products with: Production and Characterization of Monoclonal and Polyclonal Antibodies Against Truncated Recombinant Dickkopf-1 as a Candidate Biomarker.Viral antibodies: anti-H Rabbit Anti-Rat Androgen Recombinant Human Androge Anti AGO2 Human, Monoclon Anti PIWIL1, Monoclonal A Anti AGO2 Mouse, Monoclon Anti Human AGO3, Monoclon Viral antibodies, anti-R Signal Transduction Anti Signal Transduction Anti Measles Virus Nucleoprote Astrovirus antibody, Mono
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Identification and molecular characterization of a novel Babesia orientalis thrombospondin-related anonymous protein (BoTRAP1).The thrombospondin-related anonymous protein (TRAP) family, a kind of transmembrane protein, is widely distributed with a conserved feature of structure in all apicomplexan parasites and plays a crucial role in the gliding motility and survival of parasites.
1205 related Products with: Identification and molecular characterization of a novel Babesia orientalis thrombospondin-related anonymous protein (BoTRAP1).Myotubularin related prot Tubby-related protein 1 a myotubularin related prot Primary antibody low den Apoptosis Phospho-Specifi EGF Phospho-Specific Arra Mouse Anti-Human Tyrosina Rabbit Anti-Rat Androgen Mouse Anti hPTH PTH rP Ta Rat monoclonal anti mouse Rat monoclonal anti mouse Mouse Anti-LDL Receptor-R
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Synthesis in Escherichia coli and Characterization of Human Recombinant Erythropoietin with Additional Heparin-Binding Domain.Recombinant human erythropoietin (EPO) with additional N-terminal heparin-binding protein domain (HBD) from bone morphogenetic protein 2 was synthesized in Escherichia coli cells. A procedure for HBD-EPO purification and refolding was developed for obtaining highly-purified HBD-EPO. The structure of recombinant HBD-EPO was close to that of the native EPO protein. HBD-EPO contained two disulfide bonds, as shown by MALDI-TOF mass spectrometry. The protein demonstrated in vitro biological activity in the proliferation of human erythroleukemia TF-1 cell test and in vivo activity in animal models. HBD-EPO increased the number of reticulocytes in the blood after subcutaneous injection and displayed local angiogenic activity after subcutaneous implantation of demineralized bone matrix (DBM) discs with immobilized HBD-EPO. We developed a quantitative sandwich ELISA method for measuring HBD-EPO concentration in solution using rabbit polyclonal serum and commercial monoclonal anti-EPO antibodies. Pharmacokinetic properties of HBD-EPO were typical for bacterially produced EPO. Under physiological conditions, HBD-EPO can reversibly bind to DBM, which is often used as an osteoplastic material for treatment of bone pathologies. The data on HBD-EPO binding to DBM and local angiogenic activity of this protein give hope for successful application of HBD-EPO immobilized on DBM in experiments on bone regeneration.
2689 related Products with: Synthesis in Escherichia coli and Characterization of Human Recombinant Erythropoietin with Additional Heparin-Binding Domain.Recombinant Human Inhibin Recombinant Human Inhibin Recombinant Human Inhibin Bone Morphogenetic Protei Fibroblast Growth Factor Fibroblast Growth Factor Growth Differentiation Fa Macrophage Colony Stimula Macrophage Colony Stimula Macrophage Colony Stimula RANK Ligand Soluble, Huma RANK Ligand Soluble, Huma
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Lateral flow dipstick antigen assay for human cystic echinococcosis.Cystic echinococcosis (CE) is a neglected zoonotic disease with a worldwide distribution and is a major public health problem in some areas. Diagnosis of CE is mainly based on clinical symptoms, imaging and serological testing, however, improvement in serodiagnosis is still needed. This study was aimed at detecting circulating Echinococcus antigen in CE patients using a lateral flow dipstick (LFD) assay. Three types of hydatid antigens i.e. hydatid cyst fluid (HCF), native antigen B (nAgB) and recombinant antigen B (rAgB) were prepared and polyclonal rabbit antiserum was raised against each antigen. Purified IgG fractions were prepared and a portion was conjugated to gold nanoparticles. After a series of optimizations, a final antigen detection LFD assay was developed using a combination of anti-nAgB-IgG and gold-conjugated anti-HCF-IgG. Evaluation of the assay showed that 27 out of 35 (77%) serum samples from CE patients gave positive results. Meanwhile, the test showed a diagnostic specificity of 82% when tested with sera from 38 healthy individuals and 13 patients with other parasitic diseases. In conclusion, the antigen detection LFD assay seemed to be useful for diagnosis of CE and possibly for post-treatment follow-up, and merit further evaluation studies. We foresee that it may improve serodiagnosis of CE when used in tandem with an antibody detection test.
LATERAL FLOW ASSAY EZ PAN Human Antithrombin III to Complex Human PAI-1-uPA A Human Plasminogen Total A Total Human uPA Antigen A Human Vitronectin Total A Bone Morphogenetic Protei anti HBsAg surface antige anti CD54 IgG2b k monoclo Growth Differentiation Fa anti CD66e IgG1 monoclona Mouse anti-human type I c
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Detection and kinetics of persistent neutralizing anti-interferon-beta antibodies in patients with multiple sclerosis. Results from the ABIRISK prospective cohort study.Two validated assays, a bridging ELISA and a luciferase-based bioassay, were compared for detection of anti-drug antibodies (ADA) against interferon-beta (IFN-β) in patients with multiple sclerosis. Serum samples were tested from patients enrolled in a prospective study of 18 months. In contrast to the ELISA, when IFN-β-specific rabbit polyclonal and human monoclonal antibodies were tested, the bioassay was the more sensitive to detect IFN-β ADA in patients' sera. For clinical samples, selection of method of ELISA should be evaluated prior to the use of a multi-tiered approach. A titer threshold value is reported that may be used as a predictor for persistently positive neutralizing ADA.
2862 related Products with: Detection and kinetics of persistent neutralizing anti-interferon-beta antibodies in patients with multiple sclerosis. Results from the ABIRISK prospective cohort study.Rat monoclonal anti mouse Goat Anti-Human, Mouse, R Goat Anti-Human CBX1 HP1- Goat Anti-Human, Mouse In Mouse anti human Integrin Rat anti mouse LPAM-1 (In Rat monoclonal anti mouse Hamster anti mouse Interf Rat monoclonal anti mouse Multiple organ tumor tiss Rabbit Anti-Human Interfe Mouse Anti-THS, intact &
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Immunogenicity comparison of lactoferrin purified from Saudi Arabia camel clans milk.Secretory lactoferrins play a crucial rolls at mucosal surfaces as not only antimicrobial molecules in primate as well as human, but as physiological protein. Its multiple functions extended to be one of immunogen could elicited autoimmune disorders. Purified camel lactoferrin (cLfs) from different Saudi camel clans were shown to be a potent immunogen when injected into rabbit. Four rabbit were subcutaneously immunized with different camel clans lactoferrin/Freunds adjuvant. Anti-cLfs potency titration was reach 1:32000 and did not significantly differences between different cLfs. The cross-reactivity level of different anti-Lfs were highly significant, specially between cLfs and bLf/hLf.
2501 related Products with: Immunogenicity comparison of lactoferrin purified from Saudi Arabia camel clans milk.Methyl Green (Purified) Methyl Green (Purified) Casein ELISA Kit Milk ca MOUSE ANTI BOVINE ROTAVIR Endorphin, Beta (Bovine, Lipotropin (61 64) Beta ( anti Adenovirus E11 IgG2a anti Adenovirus C11 IgG2a Viral antibodies, anti-R anti Rotavirus p42 IgG2a anti TGE IgG1 (monoclonal anti HBsAg pre surface Ig
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