Search results for: Anti-Human, Mouse Monoclonal Antibody, Clone LPR-02
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Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3.The carcinoembryonic antigen, glypican‑3 (GPC3), is a putative therapeutic target and diagnostic marker of hepatoma. In the present study, a monoclonal antibody (mAb) specifically against GPC3 was obtained via cloning the sequence of GPC3 via polymerase chain reaction and inserting it into a pET16b vector prior to transfection into Escherichia coli (E. coli) BL21. BALB/c mice were immunized with 20 µg purified antigen by intrasplenic embedding. Splenocytes and mouse myeloma cells SP2/0 were fused; then, the hybridoma cells were screened by an indirect ELISA. The properties of the mAb were examined by western blotting and immunofluorescence analysis against the purified protein. The results revealed that the prokaryotic expression vector of GPC3 had been successfully generated and GPC3 was stably expressed in E. coli BL21. A stable hybridoma cell line, 2F3, was generated in the present study, which produced mAbs against GPC3. The mAb 2F3 had a high antibody titer and the isotype was identified as IgG1/κ; 2F3 hybridomas had a median chromosome number of 98. Western blot and immunofluorescence analyses revealed that 2F3 specifically recognized recombinant and native GPC3. The 2F3 clone was proposed as a stable secretor of this mAb against GPC3. The results of present study indicated that the successful preparation of recombinant GPC3 protein and an anti‑human GPC3 mouse mAb may be provide a basis for developments in the diagnosis and treatment of liver cancer.
1897 related Products with: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3.Anti Human Macrophage Sur Anti-actin associated ant anti CD66e IgG1 monoclona anti CD54 IgG2b k monoclo FDA Standard Frozen Tissu MOUSE ANTI BORRELIA BURGD FDA Standard Frozen Tissu MOUSE ANTI HUMAN EPITHELI HBsAg antibody, Monoclona Monoclonal antibody Anti AFP antibody, Monoclonal Anti-Human, Mouse Monoclo
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PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry.Podocalyxin (PODXL) is a CD34-related sialomucin and a well-known marker of embryonic stem cells. PODXL is expressed in many types of tumors including colorectal cancers, breast cancers, and brain tumors. Overexpression of PODXL is an independent predictor of progression, metastasis, and poor outcome. PODXL is also expressed in many normal cells such as renal podocytes and endothelial cells (ECs). However, high-sensitive and high-specific anti-PODXL monoclonal antibodies (mAbs) have not been established. Herein, we immunized mice with recombinant human PODXL, which was produced using LN229 glioblastoma cells. The anti-PODXL mAb, PcMab-47, reacted with endogenous PODXL-expressing cancer cell lines and normal cells independently of glycosylation in flow cytometry. Immunohistochemical analysis showed that PcMab-47 detected PODXL-expressing normal cells such as podocytes of kidney or ECs. Furthermore, PcMab-47 stained PODXL-expressing cancer cells of colon or breast cancers. These results suggest that PcMab-47 could be useful for investigating the expression and function of PODXL in cancers and normal tissues.
1162 related Products with: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry.MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI APAAP COMPLEX, MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD MOUSE ANTI CANINE DISTEMP MOUSE ANTI HUMAN CD15, Pr anti CD66e IgG1 monoclona Anti Bcl 2 Monoclonal Ant Mouse Anti-Insulin(1G11) Anti monomethyl Histone H Rat Anti-IAA Monoclonal A
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Preparation and application of a novel monoclonal antibody specific for human B7-H3.Human B7-H3 (CD276), as a new member of the B7 family has been demonstrated to mediate T cell proliferation and the production of interferon‑γ. Two isoforms of B7-H3 have been identified in humans, 2IgB7‑H3 and 4IgB7‑H3. Since the costimulatory functions of the two isoforms remains to be fully elucidated, there are disagreements regarding their expression patterns as well as the T cell responses. In the present study, a single mouse anti‑human monoclonal antibody (mAb), specific for 2IgB7‑H3 and 4IgB7‑H3 was established, termed 11F4. Using this antibody, the expression of B7‑H3 was observed extensively in tumor cell lines, with the exception of certain human hematopoietic cell lines. Subsequently, the fusion proteins of the two B7‑H3 isoforms were produced to analyze the biological function of 4IgB7‑H3 and 2IgB7‑H3 using a Cell Counting Kit‑8 assay, and the data revealed that the two isoforms exhibited a similar function in promoting T cell proliferation. In addition, the effect of B7‑H3 on the T cells was inhibited by the 11F4 mAb. Overall, the novel antibody produced was observed to exhibit an inhibitory effect offering a useful tool in further investigations of the function of B7-H3 isoforms.
1763 related Products with: Preparation and application of a novel monoclonal antibody specific for human B7-H3.MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI CANINE DISTEMP MOUSE ANTI APAAP COMPLEX, MOUSE ANTI BOVINE ROTAVIR RABBIT ANTI GSK3 BETA (pS PABP1-dependent poly A-sp Rabbit Anti-Nkx2.5 Cardia Mouse Anti-Cripto 1 monoc formin-like 1 antibody So NATIVE HUMAN PROLACTIN, P
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B7-H1 expression in malignant pleural mesothelioma is associated with sarcomatoid histology and poor prognosis.B7 homolog 1 (B7-H1; aka programmed cell death 1 ligand 1) is a negative costimulatory molecule that is associated with poor prognosis in many tumor types. Given the poor prognosis and the limited treatments available for mesothelioma, we decided to examine B7-H1 expression and its association with survival in patients with mesothelioma.
2130 related Products with: B7-H1 expression in malignant pleural mesothelioma is associated with sarcomatoid histology and poor prognosis.Rabbit Anti-Integrin β2 Rabbit Anti-intestinal FA Rabbit Anti-Integrin beta Rabbit Anti-Integrin alph Malignant melanoma tissue Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-Insulin Polyc Horizontal Laminar Flow Rabbit Anti-NOS-2 iNOS Po Rabbit Anti-Integrin alph Rabbit Anti-TNIP2 ABIN2 T Mouse Anti-Insulin-Like G
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Two novel monoclonal antibodies against human CD133-2: distinct epitopes and agonist activity to enhance growth of CD133 expression cells in vitro.Human AC133 antigen, also called CD133, is a unique transmembrane glycoprotein encoded by the PROM1 gene. It was initially suggested as a cell surface marker for hematopoietic stem/progenitor cells and has also been identified recently as a cancer stem cell marker in brain, colorectal, and prostate cancers. AC133 has two isoforms, one is AC133-1 and the other is AC133-2. Whether the two isoforms are functionally redundant or serve distinct functions remains unclear. In order to further explore the physiological and pathological functions of CD133-2, we generated two mouse antihuman CD133-2 monoclonal antibodies (clones 6B3 and 9G4). Then, we carefully characterized the biological functions of these monoclonal antibodies (MAbs) and showed that 6B3 and 9G4 bound to two epitopes of CD133, which are different from that recognized by the commercially available anti-CD133 MAb (AC141). These two MAbs could be used in cell immunostaining, Western blot, and immunohistochemical staining. We have further shown that MAb 6B3 enhances the growth of human myelogenous leukemic cell line U937 and human colon adenocarcinoma cell line SW480, suggesting a functional role of CD133 in these cells. Taken together as a useful tool, these two antibodies might be of great value for further exploration of the expression and function of CD133.
2996 related Products with: Two novel monoclonal antibodies against human CD133-2: distinct epitopes and agonist activity to enhance growth of CD133 expression cells in vitro.Goat Anti-Human Fibroblas Rat monoclonal anti mouse Rat monoclonal anti mouse Human Growth Hormone anti VEGFR-2 (Human, monoclona Growth Factor (Human) Ant Rat monoclonal anti mouse Rat monoclonal anti mouse Human Growth Hormone anti Rabbit Anti-Human Toll In VEGFR-1 (Human, monoclona Hsp90 total Monoclonals A
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Antibody internalization after cell surface antigen binding is critical for immunotoxin development.Immunotoxin potency is dependent on cell surface binding specificity as well as internalization efficiency. Current approaches for immunotoxin development are dependent on existing antibodies that were selected for high affinity and/or high production yield. However, these antibodies may demonstrate low internalization efficiency upon cell surface binding and thus are not necessarily the best candidates for immunotoxin design. Here, we have developed an assay with a novel protein, DTG3, to compare and evaluate the internalization efficiency of monoclonal antibodies in order to circumvent the possibility of low internalization. DTG3 is a fusion protein containing the N-terminus of diphtheria toxin (DT) and three copies of streptococci Protein G immunoglobulin binding domains. We show that antibody-DTG3 complexes formed in the test tube are able to bind their antigen on the target cell surface, resulting in cell internalization, DT-mediated protein synthesis inhibition, and host cell apoptosis. We tested this system with two well-studied antibodies, antihuman CD3ε, and anti-PSMA antibodies and were able to show efficiency of this assay. We further examined commercially available anti-CD123 antibodies for potential leukemia-targeting immunotoxin development. Finally, we applied this system in the early-stage screening of newly generated anti-CD123 hybridomas. Our data showed that this internalization assay system is sensitive, time efficient, and reproducible, and has provided a tool to compare monoclonal antibodies for the clinical development of effective immunotoxins for the treatment of a variety of neoplasms.
1903 related Products with: Antibody internalization after cell surface antigen binding is critical for immunotoxin development.Rabbit Anti-Cell death in Rabbit Anti-Cell death in Mouse Anti-Human CD34 Tar Rabbit Anti-cellulase Pol Mouse Anti DO11.10 T cell Rabbit Anti-cellulase Pol cell cycle progression 2 MOUSE ANTI BOVINE ROTAVIR Anti-Hepatitis B Surface amyloid beta precursor pr Rabbit Anti-Cell death in Rabbit Anti-Cell death in
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Establishment of antihuman IFN-alpha8-specific monoclonal antibodies and their application in the enzyme-linked immunosorbent assay (ELISA).In the present study, we describe the generation of a series of anti-interferon-alpha8 (IFN-alpha8)-specific monoclonal antibodies (mAbs), their characterization, and the establishment of a sandwich enzyme-linked immunosorbent assay (ELISA) system for human IFN-alpha8. The sandwich ELISA system is highly sensitive to human natural IFN-alpha8 (nIFN-alpha8), with a minimum detection limit of 50 pg/mL, which did not cross-react with the other IFN preparations and several cytokines tested. Using this ELISA system, pharmacokinetic properties of an IFN-alpha preparation administered in mice were examined. We found that IFN-alpha8 has higher vascular permeability and stability than IFN-alpha2 in the circulation. These results suggest that this ELISA would be very useful for determination of IFN-alpha8 protein concentrations in various experimental samples and also of pharmacokinetic properties of IFN-alpha preparations in human.
1467 related Products with: Establishment of antihuman IFN-alpha8-specific monoclonal antibodies and their application in the enzyme-linked immunosorbent assay (ELISA).Multiple organ tumor tiss Apoptosis (Human) Antibod MOUSE ANTI HUMAN CD19 RPE Cytokine (Mouse) Antibody Rat monoclonal anti mouse Inflammation (Mouse) Anti Atherosclerosis (Mouse) A Alkaline Phospatase (ALP) Cytokine (Human) Antibody OxiSelect™ Cellular UV- AKT PKB Signaling Phospho Cytokine (Mouse) Antibody
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Identification of zinc-alpha-2-glycoprotein binding to clone AE7A5 antihuman EPO antibody by means of nano-HPLC and high-resolution high-mass accuracy ESI-MS/MS.The detection of doping with recombinant erythropoietins (Epo) by isoelectric focusing (IEF) and Western double blotting strongly relies on the specificity of the detection antibody used. Currently a monoclonal mouse antibody (clone AE7A5) is used for that purpose. Despite its excellent sensitivity (amol range) the antibody shows some nonspecific binding behavior. However, the binding occurs outside the currently used pH range for evaluating erythropoietin IEF profiles. A shotgun proteomics approach is described consisting of preparative IEF on large-sized carrier ampholyte gels (pH 3-5), SDS-PAGE, Western single and double blotting, on-membrane elution of intact proteins, on-membrane and in-solution tryptic digestions, as well as nano-HPLC peptide separation and high-resolution high-mass accuracy ESI-MS/MS peptide sequencing. The nonspecifically interacting protein could be identified as zinc-alpha-2-glycoprotein (ZAG). Confirmation analyses were performed using recombinant ZAG (rhZAG) and a monoclonal anti-ZAG antibody. It could be demonstrated that the binding of the monoclonal antihuman EPO antibody (clone AE7A5) to ZAG occurs in a highly concentration-dependant manner and that only samples containing increased amounts of urinary ZAG lead to a detectable interaction of the AE7A5 antibody on Epo-IEF gels.
1866 related Products with: Identification of zinc-alpha-2-glycoprotein binding to clone AE7A5 antihuman EPO antibody by means of nano-HPLC and high-resolution high-mass accuracy ESI-MS/MS.Rabbit Polyclonal Antibod Sheep Polyclonal Antibody Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-AGPA Alpha 1 Cytokeratin, High Molecu Rabbit Anti-AGPA Alpha 1 Rabbit Anti-AGPB Alpha 1 Rabbit Anti-AGPB Alpha 1 pCMVLuxA Mammalian LuxA E Rabbit Anti-HDL high dens Rabbit Anti-AGPA Alpha 1
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Generation and characterization of a panel of monoclonal antibodies specific for human fibroblast growth factor receptor 4 (FGFR4).Fibroblast growth factor receptor 4 (FGFR4) is a member of the FGFR family of receptor tyrosine kinases, and plays important roles in a variety of biological functions such as cell proliferation, differentiation, migration, angiogenesis, tissue repair, and tumorigenesis. The human FGFRs share a high degree of sequence homology between themselves, as well as with their murine homologs. Consequently, it has been suggested that it may be difficult to prepare monoclonal antibodies (MAbs) that are specific for the individual receptor types. In this communication, we report on the development and characterization of a panel of anti-human FGFR4 MAbs that were generated in mice using a rapid immunization protocol. Using a modified rapid immunization at multiple sites (RIMMS) protocol with the soluble extracellular domain of human FGFR4 (FGFR4-ECD), the immunized mice developed high levels of polyclonal IgG to the immunogen within 13 days of the first immunization. The lymph node cells isolated from the immunized animals were then fused with mouse myeloma cells for hybridoma generation. Use of an efficient hybridoma cloning protocol in combination with an ELISA screening procedure allowed for early identification of stable hybridomas secreting antihuman FGFR4 IgG. Several identified MAbs specifically reacted with the FGFR4 protein without binding to the other human isoforms (FGFR1, FGFR2, and FGFR3). As evaluated by BIAcore analysis, most anti-FGFR4 MAbs displayed high affinities (8.6 x 10(8) approximately 3.9 x 10(10) M) to FGFR4. Furthermore, these MAbs were able to bind to FGFR4 expressed on human breast tumor cell lines MDA-MB-361 and MDA-MB-453. Taken together, the results demonstrate that the RIMMS strategy is an effective approach for generating class-switched, high-affinity MAbs in mice to evolutionarily conserved proteins such as human FGFR4. These MAbs may be useful tools for further investigation of the biological functions and pathological roles of human FGFR4.
1148 related Products with: Generation and characterization of a panel of monoclonal antibodies specific for human fibroblast growth factor receptor 4 (FGFR4).Goat Anti-Human Fibroblas IGF-1R Signaling Phospho- Growth Factor (Human) Ant Growth Differentiation Fa MOUSE ANTI HUMAN CD19 RPE Human Growth Hormone anti Rat monoclonal anti mouse Rat monoclonal anti mouse Human Fibroblast Growth F Fibroblast Growth Factor Rat monoclonal anti mouse Sheep Polyclonal Antibody
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A novel blocking monoclonal antibody recognizing a distinct epitope of human CD40 molecule.CD40, a member of the tumor necrosis factor receptor superfamily, is an important costimulatory molecule during the immune response. Here, we report a blocking mouse antihuman CD40 monoclonal antibody, mAb 3G3, of which the specificity was verified by flow cytometry and Western blot. It was shown by competition test that 3G3 bound to a different site (epitope) of CD40 from the reported CD40 mAbs, including clone mAb89, 3B2, and 5C11. It was also found that mAb 3G3 could inhibit homotypic aggregation of Daudi cells induced by the agonistic anti-CD40 mAb 5C11. Furthermore, mAb 3G3 effectively inhibited the proliferation of peripheral blood mononuclear cells in mixed lymphocyte reaction assay. Finally, a sensitive and specific soluble CD40 (sCD40) ELISA kit was established by matching mAb 3G3 with 5C11, and it was found that the levels of sCD40 in sera from patients with immune disorders such as hyperthyroidism, chronic nephritis, and rheumatoid arthritis were obviously higher than those from normal individuals. Thus, this blocking anti-CD40 mAb provides a novel tool for the study of CD40.
2000 related Products with: A novel blocking monoclonal antibody recognizing a distinct epitope of human CD40 molecule.Mouse Monoclonal Antibody anti CD20 monoclonal anti Anti AGO2 Human, Monoclon Human IgE antibody, Monoc Purified Mouse Anti Human Mouse monoclonal to huma MOUSE ANTI HUMAN CD15, Pr Purified Mouse Anti Human anti CD66e IgG1 monoclona Purified Mouse Anti Human Human Growth Hormone anti Anti-Human, Mouse Monoclo
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