Product name :Human Mouse Rat Phospho-Mek (S217 S221) and Total Mek ELISA ELISA

Catalog Number :PEL-Mek-S217-002

Quantity :One 96-Well Strip Microplate Kit

Price :470 Eur

Pay now with :

Supplier :Ray Biotech

 

Product Feature

  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
  • High sensitivity
  • High quality
  • Cost-effective
  • Complete kit with pre-coated 96 well strip-plate

Target Name

Mek-S217

Target Species

Human

Species Detected

Human, Mouse, Rat

Application Notes

Suggested Application
Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Pre-Coated 96-Well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C.
  3. Add 100 μl of prepared primary antibody to each well. Incubate 1 h at RT.
  4. Add 100 μl of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT.
  5. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
  6. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Mek1 (Ser217/221) + pan Mek.

Storage/Stability

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

datasheet of product :Human Mouse Rat Phospho-Mek (S217 S221) and Total Mek ELISA ELISA  Human Mouse Rat Phospho-Mek (S217 S221) and Total Mek ELISA ELISA

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294-62501 Beta Amyloid (40) ELISA Kit, Human Rat Alzheimer''s Disease (AD) is characterized by the presence of extracellular senile plaques (SPs) and intracellular neurofibrillary tangles (NFT) in the brain. Th B-Bridge 96 tests 1040.55 294-62501 Beta Amyloid (40) ELISA Kit, Human Rat Alzheimer''s Disease (AD) is characterized by the presence of extracellular senile plaques (SPs) and intracellular neurofibrillary tangles (NFT) in the brain. Th B-Bridge 96 tests 1040.55€
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294-64701 Beta Amyloid (40) ELISA Kit II, Human Rat B-Bridge 96 tests 1326.95 294-64701 Beta Amyloid (40) ELISA Kit II, Human Rat B-Bridge 96 tests 1326.95€
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294-64701 Beta Amyloid (40) ELISA Kit II, Human Rat Detection kit creates a more stable antigen antibody reaction by using F(ab'')2 fragment of the labeled antibody BA27, which recognizes the C terminal of Ab 4 B-Bridge 96 tests 1040.55 294-64701 Beta Amyloid (40) ELISA Kit II, Human Rat Detection kit creates a more stable antigen antibody reaction by using F(ab'')2 fragment of the labeled antibody BA27, which recognizes the C terminal of Ab 4 B-Bridge 96 tests 1040.55€
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bs-1945R-HRP Rabbit Anti-WNK3 protein Polyclonal Antibody, HRP Conjugated, 192(human), 198(mo), 186(rat)kDa, Isotype IgG, Application WB(1 100-500), ELISA(1 500-1000) Bioss 100ug Lyophilized 530 bs-1945R-HRP Rabbit Anti-WNK3 protein Polyclonal Antibody, HRP Conjugated, 192(human), 198(mo), 186(rat)kDa, Isotype IgG, Application WB(1 100-500), ELISA(1 500-1000) Bioss 100ug Lyophilized 530€ Pub
bs-3280R-AF350 Rabbit Anti-Phospho-MLK3(Thr277 Ser281) Polyclonal Antibody, Alexa Fluor 350 Conjugated, 90-100kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , GuineaPig , Rabbit; Application Flow Bioss 100ug Lyophilized 550 bs-3280R-AF350 Rabbit Anti-Phospho-MLK3(Thr277 Ser281) Polyclonal Antibody, Alexa Fluor 350 Conjugated, 90-100kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , GuineaPig , Rabbit; Application Flow Bioss 100ug Lyophilized 550€
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bs-3280R-AF488 Rabbit Anti-Phospho-MLK3(Thr277 Ser281) Polyclonal Antibody, Alexa Fluor 488 Conjugated, 90-100kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , GuineaPig , Rabbit; Application Flow Bioss 100ug Lyophilized 550 bs-3280R-AF488 Rabbit Anti-Phospho-MLK3(Thr277 Ser281) Polyclonal Antibody, Alexa Fluor 488 Conjugated, 90-100kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , GuineaPig , Rabbit; Application Flow Bioss 100ug Lyophilized 550€
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bs-3280R-FITC Rabbit Anti-Phospho-MLK3(Thr277 Ser281) Polyclonal Antibody, FITC Conjugated, 90-100kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , GuineaPig , Rabbit; Application Flow-Cyt(1 20- Bioss 100ug Lyophilized 550 bs-3280R-FITC Rabbit Anti-Phospho-MLK3(Thr277 Ser281) Polyclonal Antibody, FITC Conjugated, 90-100kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , GuineaPig , Rabbit; Application Flow-Cyt(1 20- Bioss 100ug Lyophilized 550€ Pub
bs-4610R-PE Rabbit Anti-phospho-AQP2(Ser264 261) Polyclonal Antibody, PE Conjugated , 32kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Sheep , Dog , Chicken , Pig; Application Flow-Cyt(1 20-100), Bioss 100ug Lyophilized 550 bs-4610R-PE Rabbit Anti-phospho-AQP2(Ser264 261) Polyclonal Antibody, PE Conjugated , 32kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Sheep , Dog , Chicken , Pig; Application Flow-Cyt(1 20-100), Bioss 100ug Lyophilized 550€ Pub
bs-4610R-PE-Cy3 Rabbit Anti-phospho-AQP2(Ser264 261) Polyclonal Antibody, PE-Cy3 Conjugated , 32kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Sheep , Dog , Chicken , Pig; Application Flow-Cyt(1 20-10 Bioss 100ug Lyophilized 577 bs-4610R-PE-Cy3 Rabbit Anti-phospho-AQP2(Ser264 261) Polyclonal Antibody, PE-Cy3 Conjugated , 32kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Sheep , Dog , Chicken , Pig; Application Flow-Cyt(1 20-10 Bioss 100ug Lyophilized 577€ Pub
bs-7555R-PE Rabbit Anti-phospho-EPH A3+A4+A5 ( Tyr779) Polyclonal Antibody, PE Conjugated , 110kDa; Isotype IgG; Reactivity Human , Rat , Mouse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 550 bs-7555R-PE Rabbit Anti-phospho-EPH A3+A4+A5 ( Tyr779) Polyclonal Antibody, PE Conjugated , 110kDa; Isotype IgG; Reactivity Human , Rat , Mouse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 550€ Pub
bs-7555R-PE-Cy3 Rabbit Anti-phospho-EPH A3+A4+A5 ( Tyr779) Polyclonal Antibody, PE-Cy3 Conjugated , 110kDa; Isotype IgG; Reactivity Human , Rat , Mouse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 577 bs-7555R-PE-Cy3 Rabbit Anti-phospho-EPH A3+A4+A5 ( Tyr779) Polyclonal Antibody, PE-Cy3 Conjugated , 110kDa; Isotype IgG; Reactivity Human , Rat , Mouse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 577€ Pub
bs-7601R-PE Rabbit Anti-phospho-IKKi IKKe(Thr500) Polyclonal Antibody, PE Conjugated , 80kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Pig; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 550 bs-7601R-PE Rabbit Anti-phospho-IKKi IKKe(Thr500) Polyclonal Antibody, PE Conjugated , 80kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Pig; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 550€ Pub
bs-7601R-PE-Cy3 Rabbit Anti-phospho-IKKi IKKe(Thr500) Polyclonal Antibody, PE-Cy3 Conjugated , 80kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Pig; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 577 bs-7601R-PE-Cy3 Rabbit Anti-phospho-IKKi IKKe(Thr500) Polyclonal Antibody, PE-Cy3 Conjugated , 80kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Pig; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 577€ Pub
CAN-TE-250 Testosterone – Total ELISA Human Serum 96 Wells Kit DBC 1 kit 176 CAN-TE-250 Testosterone – Total ELISA Human Serum 96 Wells Kit DBC 1 kit 176€
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CBEL-EGFR-1 Human Phospho-EGFR (Activated) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347 CBEL-EGFR-1 Human Phospho-EGFR (Activated) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347€ Pub
CBEL-EGFR1068-1 Human Phospho-EGFR (Y1068) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347 CBEL-EGFR1068-1 Human Phospho-EGFR (Y1068) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347€ Pub
CBEL-STAT5-1 Human Phospho-Stat 5 (Y694) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347 CBEL-STAT5-1 Human Phospho-Stat 5 (Y694) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347€ Pub
DEV9922 Corticosterone rat mouse ELISA Standard range: 15 - 2250 ng ml Gentaur 96 tests 286 DEV9922 Corticosterone rat mouse ELISA Standard range: 15 - 2250 ng ml Gentaur 96 tests 286€ Pub
ELH-PSAtotal-001 Human PSA-total ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELH-PSAtotal-001 Human PSA-total ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
IHATIIIKT-TOT Human Antithrombin III total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597 IHATIIIKT-TOT Human Antithrombin III total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597€ Pub
IHVN1H820-10mg Mouse Monoclonal to Human Vitronectin for ELISA & WB 10mg Innovative Research INC 10mg 5764 IHVN1H820-10mg Mouse Monoclonal to Human Vitronectin for ELISA & WB 10mg Innovative Research INC 10mg 5764€ Pub
IHVN1H820-1mg Mouse Monoclonal to Human Vitronectin for ELISA & WB 1mg Innovative Research INC 1mg 882 IHVN1H820-1mg Mouse Monoclonal to Human Vitronectin for ELISA & WB 1mg Innovative Research INC 1mg 882€ Pub
IMFXKT-TOT Mouse Factor X total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597 IMFXKT-TOT Mouse Factor X total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597€ Pub
IRAPKT2007 Human Total Thyroxine (T4) ELISA Kit Innovative Research INC 1 kit(96 Wells) 486 IRAPKT2007 Human Total Thyroxine (T4) ELISA Kit Innovative Research INC 1 kit(96 Wells) 486€ Pub
IRKTAH1448 Rat and Mouse Leptin ELISA Kit Innovative Research INC 1 kit(96 Wells) 420 IRKTAH1448 Rat and Mouse Leptin ELISA Kit Innovative Research INC 1 kit(96 Wells) 420€
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IRKTAH1484 Rat Mouse SPARC Osteonectin ELISA Kit Innovative Research INC 1 kit(96 Wells) 696 IRKTAH1484 Rat Mouse SPARC Osteonectin ELISA Kit Innovative Research INC 1 kit(96 Wells) 696€
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IRKTAH1493 Rat and Mouse Acrogranin ELISA Kit Innovative Research INC 1 kit(96 Wells) 696 IRKTAH1493 Rat and Mouse Acrogranin ELISA Kit Innovative Research INC 1 kit(96 Wells) 696€ Pub
IRKTAH1591 Mouse Rat Vaspin ELISA Kit Innovative Research INC 1 kit(96 Wells) 696 IRKTAH1591 Mouse Rat Vaspin ELISA Kit Innovative Research INC 1 kit(96 Wells) 696€ Pub
IRKTAH2605 Human Protein S (Total) ELISA Kit Innovative Research INC 1 kit(96 Wells) 597 IRKTAH2605 Human Protein S (Total) ELISA Kit Innovative Research INC 1 kit(96 Wells) 597€
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IRPAIKT-TOT Rat PAI-1 total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 575 IRPAIKT-TOT Rat PAI-1 total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 575€ Pub
IRPRENKT-TOT Rat Prorenin Renin Total Antigen ELISA Kit Innovative Research INC 1 kit(96 Wells) 597 IRPRENKT-TOT Rat Prorenin Renin Total Antigen ELISA Kit Innovative Research INC 1 kit(96 Wells) 597€ Pub
MTPAKT-TOT Mouse tPA total antigen ELISA kit Molecular Innovations 1 kit 593.25 MTPAKT-TOT Mouse tPA total antigen ELISA kit Molecular Innovations 1 kit 593.25€ Pub
pAP-0004 Phospho-GSK3 alpha Ser21 (human, mouse, rat) AngoiPro 50.00 ul 409 pAP-0004 Phospho-GSK3 alpha Ser21 (human, mouse, rat) AngoiPro 50.00 ul