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Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

1145 related Products with: Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

Bone Morphogenetic Protei NATIVE HUMAN PROLACTIN, P Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea Protein Purification Bea

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Bronchus-associated lymphoid tissue in pulmonary hypertension produces pathologic autoantibodies.

Autoimmunity has long been associated with pulmonary hypertension. Bronchus-associated lymphoid tissue plays important roles in antigen sampling and self-tolerance during infection and inflammation.

2623 related Products with: Bronchus-associated lymphoid tissue in pulmonary hypertension produces pathologic autoantibodies.

Alkaline Phospatase (ALP) Incu Tissue(square vessel Incu Tissue(square vessel Incu Tissue(square vessel T-2 Toxin Mycotoxins ELIS Zearalenone Mycotoxins EL Multi organ carcinoma tis Multi organ carcinoma tis Top five cancer tissue ar Pancreatic carcinoma and Multiple organs tumor and Tissue array of gastric d

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The use of melatonin in hypoxic-ischemic brain damage: an experimental study.

Oxidative stress (OS) plays a key role in perinatal brain damage. The aim of this study is to evaluate the effectiveness of melatonin as a neuroprotective drug by investigating the influence of melatonin on OS and inflammation biomarkers in an animal model of cerebral hypoxia-ischemia.

1303 related Products with: The use of melatonin in hypoxic-ischemic brain damage: an experimental study.

Anti 3 DG imidazolone Mon Brain glioblastoma tissue Goat Anti-Human CKB Brain FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Brain tumor tissue array, Brain tumor tissue array Brain tumor and adjacent

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Role of anti-TNF-α therapy in fat graft preservation.

In this study, we evaluated the role of antitumor necrosis factor-alpha (TNF-α) therapy in the decrease of adipocyte apoptosis and weight preservation of fat grafts in the rat model.

2035 related Products with: Role of anti-TNF-α therapy in fat graft preservation.

Anti beta3 AR Human, Poly Rabbit Anti-CD11b Integri Rabbit Anti-CD11b Integri Rabbit Anti-CD11b Integri Rabbit Anti-CD11b Integri Rabbit Anti-CD11b Integri Rabbit Anti-CD11b Integri Anti VGLUT 1 Rat, polyclo Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti Rat VGLUT 2, Rabbit

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LPS-Toll-Like Receptor-Mediated Signaling on Expression of Protein S and C4b-Binding Protein in the Liver.

Protein S (PS), mainly synthesized in hepatocytes and endothelial cells, plays a critical role as a cofactor of anticoagulant activated protein C (APC). PS activity is regulated by C4b-binding protein (C4BP), structurally composed of seven α-chains (C4BPα) and a β-chain (C4BPβ). In this paper, based primarily on our previous studies, we review the lipopolysaccharide (LPS)-induced signaling which affects expression of PS and C4BP in the liver. Our in vivo studies in rats showed that after LPS injection, plasma PS levels are significantly decreased, whereas plasma C4BP levels first are transiently decreased after 2 to 12 hours and then significantly increased after 24 hours. LPS decreases PS antigen and mRNA levels in both hepatocytes and sinusoidal endothelial cells (SECs), and decreases C4BP antigen and both C4BPα and C4BPβ mRNA levels in hepatocytes. Antirat CD14 and antirat Toll-like receptor (TLR)-4 antibodies inhibited LPS-induced NFκB activation in both hepatocytes and SECs. Furthermore, inhibitors of NFκB and MEK recovered the LPS-induced decreased expression of PS in both cell types and the LPS-induced decreased expression of C4BP in hepatocytes. These data suggest that the LPS-induced decrease in PS expression in hepatocytes and SECs and LPS-induced decrease in C4BP expression in hepatocytes are mediated by MEK/ERK signaling and NFκB activation and that membrane-bound CD14 and TLR-4 are involved in this mechanism.

1722 related Products with: LPS-Toll-Like Receptor-Mediated Signaling on Expression of Protein S and C4b-Binding Protein in the Liver.

p130Cas-associated protei E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran Taq SSB (Single Stranded Taq SSB (Single Stranded S100 alpha - Rabbit polyc Human S100 Calcium Bindin ribosome binding protein ribosome binding protein calcium binding protein P

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Hyperbaric oxygen inhibits ischemia-reperfusion-induced neutrophil CD18 polarization by a nitric oxide mechanism.

Hyperbaric oxygen decreases ischemia-reperfusion-induced neutrophil/intercellular adhesion molecule-1 adhesion by blocking CD18 polarization. The purpose of this study was to evaluate whether this hyperbaric oxygen effect is nitric oxide dependent and to determine whether nitric oxide synthase is required.

1536 related Products with: Hyperbaric oxygen inhibits ischemia-reperfusion-induced neutrophil CD18 polarization by a nitric oxide mechanism.

QuantiChrom™ Nitric Oxi QuantiChrom™ Nitric Oxi EnzyChrom™ Nitric Oxide Nitric Oxide Fluorometric Nitric Oxide Colorimetric Rabbit Anti-Nitric Oxide Rabbit Anti-Nitric Oxide Rabbit Anti-Nitric Oxide Nitric Oxide Synthase, mo Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon

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Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.

Closely involved in the progression of nonlinear bioimaging is the development of optical probes for investigating biological function and activity. Introduction of new fluorescent compounds possessing enhanced nonlinearities is essential for advancing the utility of two-photon absorption (2PA) processes in the biological sciences. Herein, we report the synthesis of fluorene-based fluorophores tailored for multiphoton imaging, incorporating the succinimidyl ester and thioester functionality as reactive linkers for further coupling with a wide variety of biologically relevant molecules. The succinimidyl ester amine reactive probe was conjugated with the cyclic peptide RGDfK and polyclonal antirat IgG protein. Upon conjugation, the basic molecular architecture and photophysical properties of the active 2PA chromophore remain unchanged. Conventional and two-photon fluorescence microscopy (2PFM) imaging of COS-7 and HeLa cells, incubated with either the fluorene-RGD peptide conjugate or the fluorene-IgG conjugate, was demonstrated. The fluorene-IgG conjugate was used to image cell spindles at early mitotic developmental stages.

2015 related Products with: Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.

1,1'-Dioctadecyl-3,3,3',3 Atto 550 Protein Labeling Atto 655 Protein Labeling Atto 488 RNA Labeling Kit Atto 532 RNA Labeling Kit Atto 550 RNA Labeling Kit Atto 647N RNA Labeling Ki Atto 680 RNA Labeling Kit N (NBD Aminohexanoyl) sph 5 (2 Aminoethylamino) 1 n NBD aminohexanoic acid N Pyrene 8 hydroxy 1,4,6 tr

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Fate of mu receptors during rat skeletogenesis.

Studies have intimated a role for endogenous opioids in skeletal ontogeny. We hypothesized that this role may be confined to the perinatal period. We, therefore, examined both fetal and postnatal rat long bones to determine the pattern, if any, of mu receptor expression. Perfused long bones and brains were harvested from 3 to 4 each of near-term fetuses, 3- to 4-week-old neonates, and 6-week-old juveniles. Tissues were decalcified, embedded, sectioned, quenched of peroxidase, and then blocked. Sections were incubated overnight with either rabbit antirat mu receptor IgG or naïve rabbit IgG (control) at 4 degrees C. The next day, sections were washed, blocked again, and incubated with biotin-labeled secondary antibody, streptavidin-peroxidase conjugate, and a chromogen substrate with intervening wash steps. Slides were counterstained with hematoxylin and coverslipped. Digital photomicrographs were then imported into an image analysis program and the percent area of stained cortical and trabecular bone quantified. Mu receptor expression decreased significantly with age. Approximately 25% of the area of fetal long bones stained positively, including the endosteum, periosteum, and the growth plate. Little or no nonspecific staining occurred. Staining in neonatal tissue was diminished to <11% of the area and involved areas of apparent remodeling; chondrocytes in the growth plates failed to stain. Finally, juvenile bone evidenced staining approaching background levels produced by control slides (approximately 2%). Mu receptors are abundant in developing rat long bones during fetal development but become progressively less abundant postnatally. This infers a role for endogenous opioids during skeletal ontogeny.

1601 related Products with: Fate of mu receptors during rat skeletogenesis.

Cytokine (Rat) Quantitati Cytokine (Rat) Quantitati Cytokine (Rat) Quantitati Inflammation (Rat) Quanti Human Mouse Rat Phospho-E Goat Anti-Human, Mouse, R FDA standard normal rat m FDA standard normal rat m FDA standard normal rat m Normal rat multiple organ FDA standard normal rat m Normal rat multiple organ

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New rat model induced by anti-glomerular basement membrane antibody shows severe glomerular adhesion in early stage and quickly progresses to end-stage renal failure.

The aim of the present study was to introduce a new anti-glomerular basement membrane nephritis model in which plasma creatinine levels dramatically increased only 4 weeks after a single administration of rabbit antirat glomerular basement membrane antibody in Sprague-Dawley rats. According to renal morphology, glomerular lesions characterized by mesangial expansion and adhesion of the glomerular tuft to Bowman's capsule were observed in the early stage at day 7 after disease induction; adhesion was detected in approximately 90% of glomeruli 14 days after antibody injection. After 21 days the rats exhibited pronounced glomerulosclerosis/hyalinosis and severe tubulointerstitial lesions characterized by interstitial fibrosis. Urinary podocytes excreted in nephritis rats were studied and it was found that urinary podocyte loss might be closely related to progression of renal injury. Because this new model simply and reproducibly demonstrates development of end-stage renal disease, it will be beneficial for elucidating mechanisms by which chronic renal injury irreversibly progresses, as well as for developing therapeutic agents for chronic renal failure.

1596 related Products with: New rat model induced by anti-glomerular basement membrane antibody shows severe glomerular adhesion in early stage and quickly progresses to end-stage renal failure.

Oral squamous cell cancer Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti 3 DG imidazolone Mon Anti beta3 AR Human, Poly Cytokine (Rat) Antibody A Cytokine (Rat) Antibody A Multiple organ cancer tis Lung cancer tissue array Colon cancer tissue array

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Purification and localization of nitric oxide synthases from hybrid tilapia (Nile tilapia x Mozambique tilapia).

The aims of this study were to purify and localize the nitric oxide synthases (NOSs) from hybrid tilapia (Nile tilapia Oreochromis niloticus x Mozambique tilapia O. mossambicus). The purification procedures involved affinity chromatography with a 2', 5'-ADP-agarose 4B column and ion exchange with a diethylaminoethanol Bio-Gel A column. The results from gel filtration assays showed that the molecular weights of neuronal NOS (nNOS) and inducible NOS (iNOS) were 178 and 120 kDa, respectively. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis results showed that there were three bands with molecular weights of 89, 47, and 29 kDa from the purified nNOS. However, only one band, with a molecular weight of 120 kDa, appeared on the gel from the purified iNOS. Hybrid tilapia nNOS was a dimer structure, while iNOS appeared to be a monomer structure. Moreover, our results revealed that the activities of nNOS and iNOS were significantly higher after the addition of Ca+2 or Mg+2 ions individually. However, when L-arginine and NADPH were present, the addition of 1 mM of either ion did not further increase the activity. The chemical L-N(G)-methyl-L-arginine could inhibit the activities of the purified NOSs with or without L-arginine. Western blot analyses showed only an 89-kDa immunoreactive band from the extracts of cerebrum; however, we did not find the specific bands in other tissues, such as gill, intestine, liver, spleen, and anterior kidney. We found another 120-kDa immunoreactive protein band with the rabbit antirat iNOS serum against iNOS from the extracts of anterior kidney and spleen. The results of immunohistochemistry with the rabbit antihuman nNOS serum indicated that the nNOS existed in the cerebellum, olfactory bulb, diencephalons, and nerve cell bodies and neuronal fibers of the spinal cord. Interestingly, only macrophages from anterior kidney and spleen showed positive reactions with the rabbit antirat iNOS serum. In the same way, the endothelial NOS (eNOS) located in the heart and epithelial cells of the blood vessels reacted positively with the rabbit antibovine eNOS serum.

1815 related Products with: Purification and localization of nitric oxide synthases from hybrid tilapia (Nile tilapia x Mozambique tilapia).

Rat inducible nitric oxid QuantiChrom™ Nitric Oxi QuantiChrom™ Nitric Oxi EnzyChrom™ Nitric Oxide Nitric Oxide Colorimteric Nitric Oxide Fluorometric Nitric Oxide Colorimetric Rabbit Anti-Nitric Oxide Rabbit Anti-Nitric Oxide Rabbit Anti-Nitric Oxide Nitric Oxide Synthase, mo Xylene - Peanut Oil Solu

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