Search results for: Ascorbic Acid Assay Kit
#28919468 2017/09/18 Save this To Up
A copper based enzyme-free fluorescence ELISA for HER2 detection.We reported an enzyme-free ELISA to detect breast cancer biomarker human epidermal growth factor receptor 2 (HER2) in human serum samples. Instead of enzymes (such as horseradish peroxidase) used in traditional ELISA, CuO nanoparticles were utilized as the signal probe. Compared to traditional enzymes, CuO nanoparticles have the advantages of low cost and good stability. After dissolving CuO nanoparticles with acid, the Cu (II) ions generated catalyzed the reaction of o-phenylenediamine with ascorbic acid to produce fluorescent quinoxaline derivative molecules. The immunoassay displays high sensitivity and good selectivity towards HER2 with detection limit as low as 9.65pg·mL(-1). The assay was successfully applied to the analysis of HER2 in serum of breast cancer patients. The analysis results demonstrated the HER2 level in the serum samples determined by our assay were in good agreement with those determined by commercial HER2 ELISA kit. This enzyme-free ELISA assay can be easily adapted to the detection of other analytes. With these merits, the simple, sensitive and cost effective fluorescence immunoassay shows great potential for clinical applications.
MarkerGeneTM Fluorescent Amplite™ Fluorimetric F Amplite™ Fluorimetric G Amplite™ Fluorimetric G Cell Meter™ Apoptotic a Beta Amyloid (40) ELISA K Beta Amyloid (1 42) High Beta Amyloid (1 42) ELISA 10x ELISA WASH BUFFER, Pr Human Phospho-EGFR (Activ Human Angiotensin convert EnzyChrom™ Free Fatty A
#28223550 2017/02/22 Save this To Up
NADH autofluorescence, a new metabolic biomarker for cancer stem cells: Identification of Vitamin C and CAPE as natural products targeting "stemness".Here, we assembled a broad molecular "tool-kit" to interrogate the role of metabolic heterogeneity in the propagation of cancer stem-like cells (CSCs). First, we subjected MCF7 cells to "metabolic fractionation" by flow cytometry, using fluorescent mitochondrial probes to detect PCG1α activity, as well ROS and hydrogen-peroxide (H2O2) production; NADH levels were also monitored by auto-fluorescence. Then, the various cell populations were functionally assessed for "stem cell activity", using the mammosphere assay (3D-spheroids). Our results indicate that a sub-population of MCF7 cells, with increased PGC1α activity, high mitochondrial ROS/H2O2 production and high NADH levels, all form mammospheres with a higher efficiency. Thus, it appears that mitochondrial oxidative stress and the anti-oxidant response both contribute to the promotion of mitochondrial biogenesis and oxidative metabolism in CSCs. Further validation was provided by using specific inhibitors to target metabolic processes (the NAD+ salvage pathway, glycolysis, mitochondrial protein synthesis and OXPHOS), significantly reducing CSC propagation. As a consequence, we have now identified a variety of clinically-approved drugs (stiripentol), natural products (caffeic acid phenyl ester (CAPE), ascorbic acid, silibinin) and experimental pharmaceuticals (actinonin, FK866, 2-DG), that can be used to effectively inhibit CSC activity. We discuss the use of CAPE (derived from honey-bee propolis) and Vitamin C, as potential natural therapeutic modalities. In this context, Vitamin C was ~10 times more potent than 2-DG for the targeting of CSCs. Similarly, stiripentol was between 50 to 100 times more potent than 2-DG.
1876 related Products with: NADH autofluorescence, a new metabolic biomarker for cancer stem cells: Identification of Vitamin C and CAPE as natural products targeting "stemness".Arsenic Trichloride AsCl3 MarkerGene™ LysoLive™ Anti HTLV I gp46 Clone 65 MONOBODIES (Monoclonal An Amplite™ Colorimetric N Triglyceride Assay Kit Li GLP 1 ELISA Kit, Rat Gluc Glucose Assay With the La Cultrex In Vitro Angiogen CometAssay Control Cells Neutral CometAssay Contro N-Acetyl-4,6-(p-methoxybe
#27236237 2016/07/25 Save this To Up
Microwave assisted green synthesis of fluorescent N-doped carbon dots: Cytotoxicity and bio-imaging applications.A fast and facile microwave approach for the synthesis of fluorescent nitrogen-doped carbon dots (N-CDs) is reported. The N-CDs were hydrothermally synthesized using l-ascorbic acid (AA) and β-alanine (BA) as the carbon precursor and the nitrogen dopant, respectively. The morphology of synthesized N-CDs was characterized by high resolution transmission electron microscopy (HR-TEM) and the elemental composition was analyzed using elemental mapping method. The crystallinity and graphitation of N-CDs were examined by X-ray diffraction (XRD) and Raman spectroscopy. The doping of nitrogen over the carbon dots (CDs) was revealed by attenuated total reflection conjunction with Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photo electron spectroscopy (XPS). The optical properties of synthesized N-CDs were examined by UV-Visible (UV-Vis) and fluorescence spectroscopy. The synthesized N-CDs emit strong blue fluorescence at 401nm under excitation of 325nm. The excitation dependent emission property of synthesized N-CDs was exposed from fluorescence results. The quantum yield of synthesized N-CDs is about 14% against the reference quinine sulfate. The cytotoxicity of synthesized N-CDs on Madin-Darby Canine Kidney (MDCK) and HeLa cells were evaluated through Cell Counting Kit-8 (CCK-8) cytotoxicity assay. The results implied that the fluorescent N-CDs showed less cytotoxicity, further which was successfully applied as a staining probe for the confocal imaging of MDCK and HeLa cells.
2664 related Products with: Microwave assisted green synthesis of fluorescent N-doped carbon dots: Cytotoxicity and bio-imaging applications.Enhanced Green Fluorescen Enhanced Green Fluorescen Enhanced Green Fluorescen anti GFP antibody, rat mo Fluorescent 100 bp DNA La 5 (2 Aminoethylamino) 1 n Rhodamine 123, Green Fluo 7 (Diethylamino)coumarin MarkerGene™ Multiple Dr Atto425 PCR Labeling Kit, Atto488 NT Labeling Kit, Atto488 PCR Labeling Mast
#26874032 2016/03/14 Save this To Up
Bioactivity assessment of PLLA/PCL/HAP electrospun nanofibrous scaffolds for bone tissue engineering.The purpose of this paper was to fabricate PLLA/PCL nanofibrous scaffolds containing HAP to mimic the native bone extracellular matrix for potential applications as bone tissue engineering scaffolds materials and ultimately to help the repairing of bone defects.
2871 related Products with: Bioactivity assessment of PLLA/PCL/HAP electrospun nanofibrous scaffolds for bone tissue engineering.Bone Morphogenetic Protei Alkaline Phospatase (ALP) Bone marrow tissue array, Normal bone marrow tissue Bone marrow tumor and adj Normal bone marrow tissue Bone disease spectrum (bo Bone and cartilage cancer Human normal bone tissue Human normal bone and ost Human normal bone and ost Human normal bone and ost
#26873635 2016/03/13 Save this To Up
In vitro three-dimensional coculturing poly3-hydroxybutyrate-co-3-hydroxyhexanoate with mouse-induced pluripotent stem cells for myocardial patch application.Identifying a suitable polymeric biomaterial for myocardial patch repair following myocardial infarction, cerebral infarction, and cartilage injury is essential. This study aimed to investigate the effect of the novel polymer material, poly3-hydroxybutyrate-co-3-hydroxyhexanoate, on the adhesion, proliferation, and differentiation of mouse-induced pluripotent stem cells in vitro. Mouse-induced pluripotent stem cells were isolated, expanded, and cultured on either two-dimensional or three-dimensional poly3-hydroxybutyrate-co-3-hydroxyhexanoate films (membranes were perforated to imitate three-dimensional space). Following attachment onto the films, mouse-induced pluripotent stem cell morphology was visualized using scanning electron microscopy. Cell vitality was detected using the Cell Counting Kit-8 assay and cell proliferation was observed using fluorescent 4',6-diamidino-2-phenylindole (DAPI) staining. Mouse-induced pluripotent stem cells were induced into cardiomyocytes by differentiation medium containing vitamin C. A control group in the absence of an inducer was included. Mouse-induced pluripotent stem cell survival and differentiation were observed using immunofluorescence and flow cytometry, respectively. Mouse-induced pluripotent stem cells growth, proliferation, and differentiation were observed on both two-dimensional and three-dimensional poly3-hydroxybutyrate-co-3-hydroxyhexanoate films. Vitamin C markedly improved the efficiency of mouse-induced pluripotent stem cells differentiation into cardiomyocytes on poly3-hydroxybutyrate-co-3-hydroxyhexanoate films. Three-dimensional culture was better at promoting mouse-induced pluripotent stem cell proliferation and differentiation compared with two-dimensional culture.
2879 related Products with: In vitro three-dimensional coculturing poly3-hydroxybutyrate-co-3-hydroxyhexanoate with mouse-induced pluripotent stem cells for myocardial patch application.Anti AGO2 Mouse, Monoclon Anti AGO2 Mouse, Monoclon Macrophage Colony Stimula Macrophage Colony Stimula Mouse Epstein-Barr Virus Integrin β1 (CD29) Antib TGF beta induced factor 2 Rabbit Anti-intestinal FA Rabbit Anti-APIP Apaf1 In Rabbit Anti-APIP Apaf1 In Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-TNIP2 ABIN2 T
#26826279 2016/03/19 Save this To Up
From preclinical development to clinical application: Kit formulation for radiolabelling the minigastrin analogue CP04 with In-111 for a first-in-human clinical trial.A variety of radiolabelled minigastrin analogues targeting the cholecystokinin 2 (CCK2) receptor were developed and compared in a concerted preclinical testing to select the most promising radiotracer for diagnosis and treatment of medullary thyroid carcinoma (MTC). DOTA-DGlu-DGlu-DGlu-DGlu-DGlu-DGlu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2 (CP04) after labelling with (111)In displayed excellent characteristics, such as high stability, receptor affinity, specific and persistent tumour uptake and low kidney retention in animal models. Therefore, it was selected for further clinical evaluation within the ERA-NET project GRAN-T-MTC. Here we report on the development of a pharmaceutical freeze-dried formulation of the precursor CP04 for a first multi-centre clinical trial with (111)In-CP04 in MTC patients.
1694 related Products with: From preclinical development to clinical application: Kit formulation for radiolabelling the minigastrin analogue CP04 with In-111 for a first-in-human clinical trial.succinate-CoA ligase, GDP formin-like 1 antibody So succinate-CoA ligase, ADP Bone Morphogenetic Protei Growth Differentiation Fa Amplite™ Fluorimetric F TOM1-like protein 2 antib TCP-1 theta antibody Sour TOK-1 alpha antibody Sour TOM1L1 antibody Source Ra Cultrex In Vitro Angiogen NATIVE HUMAN PROLACTIN, P
#26725933 2016/01/04 Save this To Up
Enzyme-controlled dissolution of MnO2 nanoflakes with enzyme cascade amplification for colorimetric immunoassay.A new colorimetric immunosensing platform accompanying enzyme cascade amplification strategy was fabricated for quantitative screening of small-molecular mycotoxins (aflatoxin B1, AFB1 used in this case) coupling with enzyme-controlled dissolution of MnO2 nanoflakes. The visual colored assay was executed by high-efficient MnO2-3,3',5,5'-tetramethylbenzidine (TMB) system (blue). In the presence of ascorbic acid, MnO2 nanoflakes were dissolved into Mn(2+) ions, thus resulting in a perceptible color change from blue to colorless. The reaction could be weakened through ascorbate oxidase to catalyze ascorbic acid into dehydroascorbic acid, which indirectly depended on the concentration of ascorbate oxidase. By using ascorbate oxidase/ anti-AFB1 antibody-labeled gold nanoparticles, a novel competitive-type colorimetric enzyme immunoassay was developed for detection of AFB1 on AFB1-bovine serum albumin (BSA)-conjugated magnetic beads. Upon addition of target AFB1, the analyte competed with the conjugated AFB1-BSA on the magnetic beads for the labeled anti-AFB1 antibody on the gold nanoparticles. Under optimal conditions, the absorbance decreased with increasing target AFB1 within the dynamic range of 0.05-150ngmL(-1) with a detection limit of 6.5pgmL(-1) at the 3Sblank level. The precision and specificity of the MnO2-TMB-based immunosensing system were acceptable. In addition, method accuracy was further validated for monitoring spiked peanut samples, giving results matched well with those obtained from commercialized AFB1 ELISA kit.
2743 related Products with: Enzyme-controlled dissolution of MnO2 nanoflakes with enzyme cascade amplification for colorimetric immunoassay.Cortisol Enzyme Immunoass Cortisol Enzyme Immunoass Cortisol Enzyme Immunoass Corticosterone Enzyme Imm Corticosterone Enzyme Imm PGE2 Enzyme Immunoassay k PGE2 Enzyme Immunoassay k PGE2 Enzyme Immunoassay H PGE2 Enzyme Immunoassay H PGFM Enzyme Immunoassay k PGFM Enzyme Immunoassay k Leptin ELISA Kit, Human A
#26622495 2015/12/01 Save this To Up
Characterization of human periodontal ligament cells cultured on three-dimensional biphasic calcium phosphate scaffolds in the presence and absence of L-ascorbic acid, dexamethasone and β-glycerophosphate in vitro.The aim of this study was to evaluate the effect of porous biphasic calcium phosphate (BCP) scaffolds on the proliferation and osteoblastic differentiation of human periodontal ligament cells (hPDLCs) in the presence and absence of osteogenic inducer (L-ascorbic acid, dexamethasone and β-glycerophosphate). The cell growth within the scaffolds in the absence of osteogenic inducers was studied by cell counting kit-8 (CCK-8) assay and scanning electron microscopy (SEM). Alkaline phosphatase (ALP) activity and osteoblastic differentiation markers of hPDLCs in BCP scaffolds were examined in the presence and absence of osteogenic inducers. The cell number of hPDLCs in the BCP scaffolds was less than that of hPDLCs cultured in microplates (control). SEM images showed that cells successfully adhered to the BCP scaffolds and spread amongst the pores; they also produced abundant extracellular cell matrix. In the presence and absence of osteogenic inducers, the ALP activity of hPDLCs within BCP scaffolds was suppressed in varying degrees at all time-points. In the absence of osteogenic inducers, hPDLCs in BCP scaffolds express significant higher levels of osteopontin (OPN) mRNA than the control, and there were no significant differences for Runx2 and osteocalcin (OCN) mRNA levels compared with those cultured in microplates. In the presence of osteogenic inducers, Runx2 expression levels were significantly higher than those in control. OPN and OCN mRNA levels were downregulated slightly. Three-dimensional porous BCP scaffolds are able to stimulate the osteoblastic differentiation of hPDLCs in the presence and absence of osteogenic inducer and may be capable of supporting hPDLC-mediated bone formation.
2413 related Products with: Characterization of human periodontal ligament cells cultured on three-dimensional biphasic calcium phosphate scaffolds in the presence and absence of L-ascorbic acid, dexamethasone and β-glycerophosphate in vitro.Macrophage Colony Stimula Macrophage Colony Stimula Beta Amyloid (42) ELISA K Beta Amyloid (1 40) ELISA Beta Amyloid (40) ELISA K Beta Amyloid (1 40) ELISA Human Small Intestine Mic Human Large Intestine Mic Human Internal Mammary Ar GFP Expressing Human Inte MarkerGeneTM Live Dead As Anti AGO2 Human, Monoclon
#26408866 2015/09/27 Save this To Up
In vitro antioxidant effect of Camellia sinensis on human cell cultures.Camellia sinensisis traditionally used in many polyherbal preparations for the treatment of different diseases and infections. Its action has been associated with its antioxidant activities. In this study, antioxidant effect of Camellia sinensis on hydrogen peroxide-induced human lymphocyte cell cultures was estimated. Camellia sinensis showed high contents of ascorbic acid, phenols, flavonoids, and flavonols. Good scavenging activity was evident by scavenging assays e.g. 2,2-DiPhenyl-2-Picrylhydrazyl Hydrate (DPPH), 2,2-Azinobis (3-ethyl-BenzoThiazoline-6-Sulfonic acid (ABTS) radical assay and reducing power assay. Moreover, High Performance Liquid Chromatography (HPLC-UV) chromatographs showed many notable peaks of unidentified bioactive compounds. In vitro antioxidant actions were determined by the activities of catalase (ELISA kit method), superoxide dismutase, lipid peroxidation and total protein contents on lymphocyte cell cultures. In vitro experimental trial showed strong antioxidant repair mechanism of plant against oxidative stress. Results of extraction with solvent methanol showed the highest antioxidant activity. Camellia sinensis is promising source of natural antioxidants and further studies might be a likely source of its use in remedy of different diseases.
2722 related Products with: In vitro antioxidant effect of Camellia sinensis on human cell cultures.CELLKINES Natural Human I Macrophage Colony Stimula Macrophage Colony Stimula Cultrex In Vitro Angiogen Human integrin aVb3, affi Rabbit Anti-Cell death in Rabbit Anti-Cell death in Human Small Intestine Mic Human Large Intestine Mic Human Internal Mammary Ar GFP Expressing Human Inte Goat Anti-Human Synaptogy
#26320812 2015/11/23 Save this To Up
A tri-modal molecular imaging agent for sentinel lymph node mapping.We report an "instant kit" method to radiolabel fluorescent-tilmanocept with (68)Ga and (99m)Tc for tri-modal molecular imaging of sentinel lymph nodes (SLNs).
Growth Differentiation Fa Colon cancer, metastasize Ovary serous papillary ad Breast cancer tissue arra Tissue array of breast ca Breast cancer and matched Breast cancer, carcinoma Breast cancer tissue micr Breast fibroadenoma tissu Tissue array of breast ca Breast cancer tissue arra Colorectal carcinoma and
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