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#38498503   2024/02/14 To Up

Synergistic Influence of Fungi Inoculation with Nanoparticle Foliar Application Enhances Chili ( L.) Antioxidant Enzymes, Anatomical Characteristics, and Productivity under Cold-Stress Conditions.

In this study, we aimed to evaluate the effects of fungus (AMF) inoculation, foliar application of zinc oxide and selenium nanoparticles (ZnO-NPs and Se-NPs), and their combined interactions on the growth and productivity of chili pepper under cold-stress conditions. Two field experiments were successfully conducted during the winter seasons of 2021 and 2022 in an experimental field at the Faculty of Agriculture, Cairo University, Giza, Egypt. The results showed that, under cold stress, the combination of AMF inoculation and ZnO-NPs + Se-NPs as a foliar spray increased the average fruit weight by 92.4% and 98.7%, and the number of fruits by 34.6% and 54.8 compared to control treatment in the 2021 and 2022 seasons, respectively. Additionally, the combination of AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) significantly increased the total marketable yield by 95.8% and 94.7% compared to the control, which recorded values of 2.4 and 1.9 kg m in the 2021 and 2022 seasons, respectively. Furthermore, the combination of AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) showed the highest total content of ascorbic acid and capsaicin in chili fruits compared to the other treatments. The combination of AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) stimulated the accumulation of peroxidase (POD) and nitrogen glutamate dehydrogenase (GDH) while decreasing hydrogen peroxide (HO) and lipid peroxidation (MDA) contents. SDS analysis revealed that the application of ZnO-NPs, Se-NPs, AMF + ZnO-NPs, and AMF + ZnO-NPs + Se-NPs induced the emergence of new protein bands and reconstitution of those damaged by cold stress. Regarding histological structure, the combination of AMF inoculation and ZnO-NPs + Se-NPs as a foliar spray showed an enhancement in the thickness of grana thylakoids and increased the number of chloroplasts. Intriguingly, the findings showed that AMF and a mixture of nanoparticles (ZnO-NPs + Se-NPs) could offer guidance for increasing plant development and productivity under cold-stress conditions.
Eman G Sayed, S F Desoukey, Abeer F Desouky, Mervat F Farag, Ragab I El-Kholy, Samah N Azoz

1250 related Products with: Synergistic Influence of Fungi Inoculation with Nanoparticle Foliar Application Enhances Chili ( L.) Antioxidant Enzymes, Anatomical Characteristics, and Productivity under Cold-Stress Conditions.

100ul 100ul0.1ml 100ul10 mg 100ul 100ul 100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized 100ul

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#38496978   2024/02/29 To Up

Phytochemical Screening, Molecular Docking, ADME Properties, and Antioxidant, Anticancer, and Antidiabetic Activity of Marine Halophyte (L.) Dumort.

Medicinally valuable components derived from natural resources are highly desirable as prospective alternatives to synthetic drugs to treat fatal diseases, such as cancer and diabetes mellitus. (L.) Dumort () () is a halophyte plant that can thrive in saline environments and possesses excellent medicinal properties. Hence, for the present investigation, has been chosen, and its phytochemical constituents have been extracted utilizing various solvents, including hexane, acetone, and methanol, and identified by GC-MS, LC-MS, and HPLC analyses. The antioxidant activity of the compounds using DPPH, ABTS, and reducing power assays demonstrated that all three extracts of possessed significant radical scavenging activity comparable to standard ascorbic acid with lower IC values (69.20-95.58 μg/mL). In addition, the evaluation of antidiabetic activity by α-amylase inhibition and α-glucosidase inhibition methods revealed that the acetone extract of (SMAE) displayed equipotent activity of standard acarbose with an IC of 32.6 μg/mL. Advantageously, SMAE also exhibited better inhibition activity against the growth of lung cancer cells with an IC of 78.19. μg/mL and less toxicity on the noncancerous HUVEC cells with a high IC of 300 μg/mL. In addition, the cancer cell death mechanism via the apoptotic pathway induced by SMAE was confirmed by DAPI staining and ROS analysis. The analysis of ADME properties, including absorption, distribution, metabolism, and excretion, witnessed that the physicochemical and druglikeness factors were best catered by stigmasterol, γ-sitosterol, and vitamin E. Further, the key phytochemicals identified from SMAE were docked with CtBP1 and SOX2 bound to importin-α target proteins associated with carcinogenic pathways using Schrodinger software. The results showed that the phytochemicals, scilicet, stigmasterol, γ-sitosterol, octadecadienoic acid, and vitamin E, showed a good binding affinity with Glide scores in the range -2.845-4.018 kcal/mol. Overall, the findings support that the least investigated traditional edible medicinal mangrove-related is high in pharmacologically active constituents and might be one of the finest sources of naturally derived molecules for drug development and delivery systems.
Sampath Manojkumar, Murugesan Thandeeswaran, Sathiya Kamatchi Thangavel, Annavi Arjunan, Manickam Muthuselvam, Giriraj Kalaiarasi, Kapildev Gnanajothi

1993 related Products with: Phytochemical Screening, Molecular Docking, ADME Properties, and Antioxidant, Anticancer, and Antidiabetic Activity of Marine Halophyte (L.) Dumort.

100ug200ul50 ug 25 mg10 ug1000 tests10 mg100ug10 mg5mg100 mg1,000 tests

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#38487287   2023/12/12 To Up

Exploring the biofunctionalities of lactic fermented cactus pear ( Mill.) fruit beverage: an exotic superfood.

Cactus pear fruit is known with many health benefits in ethnomedicine of countries like Mexico, Portugal, Chine, India etc. The study was aimed to develop biofunctional lactic fermented cactus pear fruit beverage to add values to the medicinal fruit. The processing parameters such as quantity of freeze dried cactus pear fruit powder, sucrose and incubation time were optimised using response surface methodology. The optimized product was then subjected to proximate compositional, physicochemical, biofunctional and microbial analysis. The lactic fermented cactus pear fruit beverage was prepared by mixing 12% [w/v] freeze dried cactus pear fruit powder and 3% sucrose in water, then pasteurised and inoculated with 3% MTCC 25515 and M9, then incubated at 37 °C for 6 h. The moisture content of the beverage was 87.77% and major constituent was carbohydrate (9.58% per wet matter basis). The 100 mL beverage contains 89.84 mg GAE phenolic compounds, 5.86 mg QE flavonoids, 71.82 mg betacyanin, 28.08 mg betaxanthin, 10.59 mg ascorbic acid. The beverage also exhibited 58% ABTS antioxidant activity. The beverage was shelf stable for 20 days at 7 ± 1 °C. Such a biofunctional beverage loaded with antioxidant potential can be consumed as refreshing drink.
Mital R Kathiriya, Yogesh Vekariya, Subrota Hati

2137 related Products with: Exploring the biofunctionalities of lactic fermented cactus pear ( Mill.) fruit beverage: an exotic superfood.

1 ml0.1 mg1 ml100.00 ul 100ul100.00 ul

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#38482509   2024/03/06 To Up

Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of : A Combined and Study.

is a therapeutic plant traditionally used to treat rabies, snake bite, diarrhea, and wound healing. To address the bioactive compounds exhibiting these activities, we performed a comprehensive study on the roots of the plant. Thus, the present study aims to inspect the antioxidant and antibacterial efficacies of compounds isolated from the combined dichloromethane : methanol (1 : 1) and methanol extracts of along with the study of their interaction with selected protein targets. The silica gel column chromatography technique was used for the isolation of compounds, and the antibacterial and antioxidant activities were evaluated using agar disc diffusion and DPPH radical scavenging assays, respectively. Furthermore, molecular docking screening, pharmacokinetics, and toxicity protocols of the compound isolates were performed to offer the potential applications of the compounds in developing novel medications. A BIOVIA Discovery Studio in combination with AutoDock Vina 4.2 software, SwissADME, and ProTox-II prediction web tools were used to generate the molecular docking, pharmacokinetics, and toxicity profiles, respectively. Notably, the chromatographic separation of the combined extracts yielded six known compounds, namely, sitosterol (), 3-hydroxyisoagatholactone (), -viniferin (), myricetin (), tricuspidatol A (), and parthenocissin A (). The antibacterial activities revealed the highest inhibition zone by tricuspidatol A () (16.67 ± 0.47), showcasing its potent activity against at 2 mg/mL, compared to ciprofloxacin (21.50 ± 0.41). -Viniferin () (IC: 0.32 g/mL) exhibited greater antioxidant activity than the others and displayed promising results compared to ascorbic acid (0.075 g/mL). The molecular docking study revealed the highest binding affinity by viniferin () (-9.9 kcal/mol) against topoisomerase II 3-Hydroxyisoagatholactone () and -viniferin () fulfilled Lipinski's rule with no violation, and the organ toxicity predictions revealed that all the compounds showed no cytotoxicity and hepatotoxicity effects. Thus, this study's combined and outcomes suggest the potential use of the isolated compounds in drug discovery and support the traditional relevance of .
Hadush Gebrehiwot, Yadessa Melaku, Muhdin Aliye, Urgessa Ensermu, Aman Dekebo, Milkyas Endale, Daniel Rentsch, Mo Hunsen

1616 related Products with: Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of : A Combined and Study.

5 G200 5 G100ug25 mg96 wells (1 kit)100ug1 g10 mg100 mg100ul200ul

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#38479919   2024/02/20 To Up

The association between vitamin C intake and the risk of abdominal aortic calcification: A population-based study.

The beneficial effects of dietary vitamin C intake on human health have received widespread attention from the population. However, the correlation between vitamin C intake and abdominal aortic calcification remains unclear. The authors aimed to investigate the relationship between dietary vitamin C intake and AAC in US adults.
Chang Liu, Hao Zhang, Yuwei Yang, Yan Cao, Dan Liang

1836 related Products with: The association between vitamin C intake and the risk of abdominal aortic calcification: A population-based study.

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#38475707   2024/03/13 To Up

Role of gamma-irradiated sodium alginate on growth, physiological and active components of iceberg lettuce (Lactuca sativa) plant.

One of the most widely recognized biostimulators of plant development; is oligoalginate, which regulates the biological processes of plants and was used in horticultural fields as a plant growth regulator. The plan of the current research was to study, however, the foliar application of un-irradiated and irradiated Na-alginate (UISA and ISA) to improve the growth, physiological activity, and other active components of the Egyptian iceberg lettuce plant. Degraded Na-alginate is equipped with exposure of sodium alginate in its solid state to gamma-rays at different dose levels (0.0, 25, 50, 75, and 100 kGy). The characterization of the oligo-alginates achieved by γ-radiation deprivation at different dose levels was performed by FTIR, XRD, TGA, SEM, and TEM. Different concentrations of irradiated sodium alginate at dose levels of 100 kGy (200, 400, 600, and 800 ppm, as well as deionized water used as a control) were sprayed with a hand sprayer every week after transplanting the iceberg lettuce seedlings in the field until the harvest stage. Morphological traits were evaluated, as well as pigments, ascorbic acid, phenols, flavonoids, soluble proteins, and antioxidant activity.
Amina A Aly, Noha E Eliwa, Gehan Safwat

1044 related Products with: Role of gamma-irradiated sodium alginate on growth, physiological and active components of iceberg lettuce (Lactuca sativa) plant.

5 x 50 ug50 ug5 x 50 ug50 ug50 ug5 μg50 ug5 x 50 ug100mg5 x 50 ug5 x 50 ug

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#38473948   2024/02/26 To Up

Transcriptomic and Physiological Analysis of the Effects of Exogenous Phloretin and Pterostilbene on Resistance Responses of against Anthracnose.

Anthracnose caused by is a destructive disease of (stylo). Combination treatment of phloretin and pterostilbene (PP) has been previously shown to effectively inhibit the conidial germination and mycelial growth of in vitro. In this study, the effects of PP treatment on the growth of in vivo and the biocontrol mechanisms were investigated. We found that exogenous PP treatment could limit the growth of and alleviate the damage of anthracnose in stylo. Comparative transcriptome analysis revealed that 565 genes were up-regulated and 239 genes were down-regulated upon PP treatment during the infection by . The differentially expressed genes were mainly related to oxidative stress and chloroplast organization. Further physiological analysis revealed that application of PP after inoculation significantly reduced the accumulation of O level and increased the accumulation of antioxidants (glutathione, ascorbic acid and flavonoids) as well as the enzyme activity of total antioxidant capacity, superoxide dismutase, catalase, glutathione reductase, peroxidase and ascorbate peroxidase. PP also reduced the decline of chlorophyll a + b and increased the content of carotenoid in response to infection. These results suggest that PP treatment alleviates anthracnose by improving antioxidant capacity and reducing the damage of chloroplasts, providing insights into the biocontrol mechanisms of PP on the stylo against anthracnose.
Shizi Zhang, Yunfeng Xu, Fang Wang, Liyun Yang, Lijuan Luo, Lingyan Jiang

2284 related Products with: Transcriptomic and Physiological Analysis of the Effects of Exogenous Phloretin and Pterostilbene on Resistance Responses of against Anthracnose.

5 G1000 tests100ug100ug10 mg10 mg100ul500 mg25 mg 5 G100ul 5 G

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#38472004   2024/02/28 To Up

Neuropathic pain in burn patients - A common problem with little literature: A systematic review.

The prevalence of neuropathic pain (NP) in burn patients is reported in the literature to be as high as 80%. Given the complexity of NP in burn patients and the wide range of treatments available, a systematic review of the literature is warranted to summarize our current understanding of management and treatment of NP in this population.
Eloise Stanton, Paul Won, Artur Manasyan, Sandeep Gurram, T Justin Gilllenwater, Haig A Yenikomshian

2398 related Products with: Neuropathic pain in burn patients - A common problem with little literature: A systematic review.

100 μg100ug100 μg100ug100 μg1 Set100 μg1 Set100 μg1 Set

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#38471573   2024/03/11 To Up

Flavonoid glycosides from the leaves of Michelia champaca.

Michelia champaca L. (Magnoliaceae) was cultivated in large scale for flowers as cosmetic raw materials, whereas the value of its leaves remains to be discovered. Our chemical study on the leaves yielded four new flavonol diglycosides, champaflavosides A-D (1-4), together with twenty-three known flavonoid glycosides (5-27). Their structures were determined by spectroscopic and chemical methods. Compounds 5-21 and 23-27 were not previously reported from the genus Michelia, and kaempferol 3-O-rutinoside (22) was obtained from this species for the first time. All the compounds were evaluated for antioxidant activity by four in vitro assays. Compounds 3-12 and 20 showed more potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than l-ascorbic acid (l-AA). Compounds 2-23, 25, and 27 exhibited 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity superior to l-AA. The ferric reducing antioxidant powers (FRAP) of compounds 2-13, 17, and 19 were higher than l-AA. Further, eighteen compounds demonstrated cellular reactive oxygen species (ROS) scavenging activity, of which champaflavoside D (4), rhamnetin 3-O-neohesperidoside (8), quercetin 3-O-(6-O-E-p-coumaroyl)-neohesperidoside (9), and liquiritin (27) were more potent than curcumin. The results revealed that the renewable leaves of M. champaca are a rich source of flavonoids and antioxidants.
Meihong Liu, Yongxia Jia, Haihui Xie

2543 related Products with: Flavonoid glycosides from the leaves of Michelia champaca.

0.1 mg 5 G100 IU500 Units 100 G121100.00 ul

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#38468328   2024/03/11 To Up

An efficient protocol for extracting thylakoid membranes and total leaf proteins from Posidonia oceanica and other polyphenol-rich plants.

The extraction of thylakoids is an essential step in studying the structure of photosynthetic complexes and several other aspects of the photosynthetic process in plants. Conventional protocols have been developed for selected land plants grown in controlled conditions. Plants accumulate defensive chemical compounds such as polyphenols to cope with environmental stresses. When the polyphenol levels are high, their oxidation and cross-linking properties prevent thylakoid extraction.
Quentin Charras, Pascal Rey, Dorian Guillemain, Fabian Dourguin, Hugo Laganier, Sacha Peschoux, Roland Molinié, Marwa Ismaël, Stefano Caffarri, Catherine Rayon, Colette Jungas

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