Search results for: Bacillus anthracis (Anthrax) lethal factor -BIOTIN Antibody
#22120797 // To Up
Detection of anthrax toxin genetic sequences by the solid phase oligo-probes.
There is an urgent need to detect a rapid field-based test to detect anthrax. We have developed a rapid, highly sensitive DNA-based method to detect the anthrax toxin lethal factor gene located in pXO1, which is necessary for the pathogenicity of Bacillus anthracis.K C Addanki, M Sheraz, K Knight, K Williams, D G Pace, O Bagasra
1192 related Products with: Detection of anthrax toxin genetic sequences by the solid phase oligo-probes.
100 μg125 mg5 mg 5 G1 mg1 mg10x96 well plates1 mg5 mg10 mgRelated Pathways
#8135809 // To Up
Structure-function analysis of Bacillus anthracis edema factor by using monoclonal antibodies.
Edema toxin of Bacillus anthracis is composed of protective antigen (PA) and edema factor (EF), a calcium- and calmodulin-dependent adenylate cyclase. At least five different antigenic regions of EF were identified using a competitive-binding, enzyme-linked immunosorbent assay of paired monoclonal antibodies (mAbs). Two mAbs, 9F5 and 7G10, inhibited the binding of 125I-EF to cell-bound PA. However, only 9F5 inhibited the elongation response of Chinese hamster ovary cells in the presence of edema toxin. Cleavage of EF at the two aspartic acid-proline residues by acid hydrolysis resulted in three fragments: a C-terminal 17 kDa fragment, a central 53 kDa fragment, and an N-terminal 18 kDa fragment. Immunoblots of EF cleaved by formic acid mapped mAbs 9F5 and 7G10 to the N-terminal 18 kDa fragment, mAb 1E6 to the C-terminal 17 kDa fragment, and the remaining 7 mAbs to the central 53 kDa fragment. mAbs 7G10 and 9F5 defined an antigenic region within amino acids 1-156 of EF which is involved in interaction with PA in forming edema toxin.S F Little, S H Leppla, J W Burnett, A M Friedlander
2391 related Products with: Structure-function analysis of Bacillus anthracis edema factor by using monoclonal antibodies.
1 mg1 mg1 mg1 mg1 mg100 ug200.00 ug1 mg200.00 ug100.00 ug1 mg100ugRelated Pathways
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