Search results for: Blocking Buffer Antibody Array
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On-chip electrochemical immunoassay platform for specific protein biomarker estimation in undiluted serum using off-surface membrane matrix.The manuscript presents a new biosensor platform using bioreceptors modified porous 2-dimensional (2D) membrane based off-surface matrix for on-chip electrochemical immunoassay. Antibody based bioreceptors modified 2D matrix of porous polycarbonate (PC) membrane with densely packed 20µm holes as off-surface matrix was incorporated in very close proximity of the sensor surface and integrated with fluidic system for reagent flow and incubation chamber. Covalent attachment of antibodies on 2D PC membrane based off-matrix was achieved using 4-fluoro-3-nitro-azidobenzene (FNAB) cross-linker. Anti-TNF-α/FNAB/PC membrane was integrated over array of micro fingers of gold based sensor chip using double side tape spacer and StartingBlock phosphate buffer saline- Tween-20; (PBS-T20) blocking buffer was utilized to minimize nonspecific binding. Differential pulse voltammetric studies of Anti-Tnf-α/FNAB/PC-Au for protein biomarker (TNF-α) detection and estimation in undiluted serum indicated that the immunosensor system can detect TNF-α linearly in 100pg/ml to 100ng/ml range with insignificant interference from other cytokines and serum proteins. Further, immunosensor exhibited high sensitivity of 194nA/(ng/ml) and 240nA/(ng/ml), respectively for single and double membrane based system. Thus, use of 2D membrane based off surface matrix may present the new platform to sensitively measure biomarkers electrochemically to pg/ml range with insignificant nonspecific binding and false signal in undiluted serum.
2161 related Products with: On-chip electrochemical immunoassay platform for specific protein biomarker estimation in undiluted serum using off-surface membrane matrix.NATIVE HUMAN PROLACTIN, P Apoptosis Phospho-Specifi EGF Phospho-Specific Arra Nuclear Membrane Receptor Angiogenesis (Human) Anti Angiogenesis (Human) Anti Angiogenesis (Mouse) Anti Apoptosis (Human) Antibod Atherosclerosis (Human) A Atherosclerosis (Mouse) A Chemokine (Human) Antibod Cytokine (Human) Antibody
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Detection of embryonic stem cell lysate biomarkers by surface plasmon resonance with reduced nonspecific adsorption.Surface plasmon resonance imaging (SPRi) has emerged as a versatile biosensor to detect a wide range of biomolecular interactions with divergent potential applications. However, the use of this advanced-level technology for stem cell lysate study is still not much explored. Cell lysates are significant biological analytes used for disease diagnostics and proteomic studies, but their complex nature limits their use as an analyte for SPRi biosensors. Here, we review the problems associated with the use of SPRi for stem cell lysate study and examine the role of surface chemistry, running buffer, and blocking solution in order to minimize nonspecific adsorption (NSA). We detect the expression of Oct4, Sox2, Nanog, Rex1, and Lin28 biomarkers present in mouse embryonic stem cell (mESC) lysate against their corresponding antibodies immobilized on the sensor surface with reduced NSA. The current study shows that the conjunction of SPRi and microarray can be used as a label-free, high-throughput, and rapid technique for detection of biomarkers and their relative abundance in stem cell lysate study.
1963 related Products with: Detection of embryonic stem cell lysate biomarkers by surface plasmon resonance with reduced nonspecific adsorption.129 Mouse Embryonic Stem Human Stem Cell Factor SC Macrophage Colony Stimula Macrophage Colony Stimula Mouse Stem Cell Factor SC Cell Meter™ Apoptotic a Cell Meter™ Apoptotic a Cell Meter™ Live Cell C Jurkat cell Lysate (untre Jurkat cell Lysate (untre Jurkat cell Lysate (Campt Jurkat cell Lysate (Campt
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Specific probe selection from landscape phage display library and its application in enzyme-linked immunosorbent assay of free prostate-specific antigen.Probes against targets can be selected from the landscape phage library f8/8, displaying random octapeptides on the pVIII coat protein of the phage fd-tet and demonstrating many excellent features including multivalency, stability, and high structural homogeneity. Prostate-specific antigen (PSA) is usually determined by immunoassay, by which antibodies are frequently used as the specific probes. Herein we found that more advanced probes against free prostate-specific antigen (f-PSA) can be screened from the landscape phage library. Four phage monoclones were selected and identified by the specificity array. One phage clone displaying the fusion peptide ERNSVSPS showed good specificity and affinity to f-PSA and was used as a PSA capture probe in a sandwich enzyme-linked immunosorbent assay (ELISA) array. An anti-human PSA monoclonal antibody (anti-PSA mAb) was used to recognize the captured antigen, followed by horseradish peroxidase-conjugated antibody (HRP-IgG) and o-phenylenediamine, which were successively added to develop plate color. The ELISA conditions such as effect of blocking agent, coating buffer pH, phage concentration, antigen incubation time, and anti-PSA mAb dilution for phage ELISA were optimized. On the basis of the optimal phage ELISA conditions, the absorbance taken at 492 nm on a microplate reader was linear with f-PSA concentration within 0.825-165 ng/mL with a low limit of detection of 0.16 ng/mL. Thus, the landscape phage is an attractive biomolecular probe in bioanalysis.
1211 related Products with: Specific probe selection from landscape phage display library and its application in enzyme-linked immunosorbent assay of free prostate-specific antigen.Prostate cancer, hyperpla PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A Mouse Anti-Prostate Speci Mouse Anti-Insulin-Like G Human Prostate Specific A Prostate-Specific Antigen PSAP (Prostate Specific
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Protein expression profiling by antibody array analysis with use of dried blood spot samples on filter paper.Dried blood spot samples (DBSS) on filter paper offer several advantages compared to conventional serum/plasma samples: they do not require any phlebotomy or separation of blood by centrifugation; they are less invasive; they allow sample stability and shipment at room temperature; and they pose a negligible risk of infection with blood-borne viruses, such as HIV, HBV and HCV, to those who handle them. Therefore dried blood spot samples (DBSS) on filter paper can be a quick, convenient and inexpensive means of obtaining blood samples for biomarker discovery, disease screening, diagnosis and treatment monitoring in non-hospitalized, public health settings. In this study, we investigated for the first time the potential application of dried blood spot samples (DBSS) in protein expression profiling using antibody array technology. First, optimal conditions for array assay performance using dried blood spot samples (DBSS) was established, including sample elution buffer, elution time, elution temperature and assay blocking buffer. Second, we analyzed dried blood spot samples (DBSS) using three distinct antibody array platforms, including sandwich-based antibody arrays, quantitative antibody arrays and biotin-label-based antibody arrays. In comparison with paired serum samples, detection of circulating proteins in dried blood spot samples (DBSS) correlated well for both low- and high-abundance proteins on all three antibody array platforms. In conclusion, our study strongly indicates the novel application of multiplex antibody array platforms to analyze dried blood spot samples (DBSS) on filter paper represents a viable, cost-effective method for protein profiling, biomarker discovery and disease screening in a large, population-based survey.
2789 related Products with: Protein expression profiling by antibody array analysis with use of dried blood spot samples on filter paper.Obesity (Human) Quantitat Obesity (Human) Quantitat Angiogenesis (Human) Quan Angiogenesis (Human) Quan Angiogenesis (Human) Quan Chemokine (Human) Quantit Cytokine (Human) Quantita Cytokine (Human) Quantita Cytokine (Human) Quantita Cytokine (Human) Quantita Cytokine (Human) Quantita Growth Factor (Human) Qua
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A versatile protein microarray platform enabling antibody profiling against denatured proteins.We aim to develop a protein microarray platform capable of presenting both natural and denatured forms of proteins for antibody biomarker discovery. We will further optimize plasma screening protocols to improve detection.
2325 related Products with: A versatile protein microarray platform enabling antibody profiling against denatured proteins.Anti Galectin(Gal 3) Huma Anti AGE 3 Monoclonal Ant MarkerGeneTM FITC Antibod MarkerGeneTM TAMRA Antibo MarkerGeneTM Biotin X Ant Anti-Acetylated Proteins Anti C Reactive Protein A ReadiLink™ mFluor™ Vi ReadiLink™ mFluor™ Vi ReadiLink™ mFluor™ Vi ReadiLink™ mFluor™ Vi ReadiLink™ mFluor™ Vi
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Comparison of techniques to screen and characterize bacteria-specific hybridomas for high-quality monoclonal antibodies selection.Antibodies are very important materials for diagnostics. A rapid and simple hybridoma screening method will help in delivering specific monoclonal antibodies. In this study, we systematically developed the first antibody array to screen for bacteria-specific monoclonal antibodies using Listeria monocytogenes as a bacteria model. The antibody array was developed to expedite the hybridoma screening process by printing hybridoma supernatants on a glass slide coated with an antigen of interest. This screening method is based on the binding ability of supernatants to the coated antigen. The bound supernatants were detected by a fluorescently labeled anti-mouse immunoglobulin. Conditions (slide types, coating, spotting, and blocking buffers) for antibody array construction were optimized. To demonstrate its usefulness, antibody array was used to screen a sample set of 96 hybridoma supernatants in comparison to ELISA. Most of the positive results identified by ELISA and antibody array methods were in agreement except for those with low signals that were undetectable by antibody array. Hybridoma supernatants were further characterized with surface plasmon resonance to obtain additional data on the characteristics of each selected clone. While the antibody array was slightly less sensitive than ELISA, a much faster and lower cost procedure to screen clones against multiple antigens has been demonstrated.
2130 related Products with: Comparison of techniques to screen and characterize bacteria-specific hybridomas for high-quality monoclonal antibodies selection.MOUSE ANTI BOVINE ROTAVIR Shiga Toxin 1 antibody, M Shiga Toxin 2 antibody, M Cholera toxin antibody, M Clostridium botulinum D T Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi
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[Optimizing the detection of soluble transferrin receptor with protein microarray].To optimize the conditions of protein microarray assay for soluble transferrin receptor (sTfR).
1458 related Products with: [Optimizing the detection of soluble transferrin receptor with protein microarray].Soluble Transferrin Recep Alpha-soluble NSF attachm RANK Ligand Soluble, Huma G protein-coupled recepto Recombinant Human Soluble Recombinant Human Soluble Recombinant Human Soluble Recombinant Human PKC the Recombinant Human PKC the Recombinant Human PKC the Recombinant Human Soluble Recombinant Human Soluble
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Analysis conditions for proteomic profiling of mammalian tissue and cell extracts with antibody microarrays.Antibody microarrays are a developing tool for global proteomic profiling. A protocol was established that permits robust analyses of protein extracts from mammalian tissues and cells rather than body fluids. The factors optimized were buffer composition for surface blocking, blocking duration, protein handling and processing, labeling parameters like type of dye, molar ratio of label versus protein, and dye removal, as well as incubation parameters such as duration, temperature, buffer, and sample agitation.
1821 related Products with: Analysis conditions for proteomic profiling of mammalian tissue and cell extracts with antibody microarrays.AccuPrep Genomic DNA Extr Small cell lung carcinoma Non small cell lung carci Lung small cell carcinoma Multiple lung carcinoma ( Astra Blue 6GLL, Stain fo Cellufine Formyl , 50 ml Cellufine Formyl Media Cellufine Formyl , 500 ml Cellufine Formyl Media Cellufine Formyl Media Anti C Reactive Protein A
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Development of antibody array for simultaneous detection of foodborne pathogens.Pathogenic bacterial contaminations present serious problems for food industry and public health. Rapid, accurate and affordable assays are needed. In this study, antibody arrays to simultaneously detect two foodborne pathogenic bacteria (Escherichia coli O157:H7 and Salmonella spp.) have been developed using chemiluminescent detecting system. Solid supports using nitrocellulose membrane and poly-l-lysine (PLL) glass slide were compared and optimized for antibody array construction. Many parameters including optimal concentrations of antibodies, blocking reagents, assay time, storage time, sensitivity and cross-reactivity were considered during optimization. This study revealed that the PLL slide was a more suitable support due to highly accurate results and the absence of non-specific background. Phosphate-buffered saline (PBS, pH 7.2) and 3% skim milk in PBS buffer were optimal spotting and blocking reagents, respectively. With the same sensitivity for bacterial detection as in a conventional ELISA (10(5)-10(6)CFU/ml for the E. coli O157:H7 and 10(6)-10(7)CFU/ml for Salmonella detections), this antibody array has advantages of a much shorter assay time of 1h and much lower required amounts of antibodies. Moreover, there was no cross-reactivity in the detection among bacteria tested in this study. Bacteria detection in food sample was feasible as demonstrated using bacteria-added milk.
2725 related Products with: Development of antibody array for simultaneous detection of foodborne pathogens.Detection Buffer A&B Anti Detection Buffer C&D Anti Antibody array detection MOUSE ANTI BOVINE ROTAVIR Blocking Buffer Antibody Streptavidin [+HRP] Antib Lysis Buffer Antibody Arr 8 Well Tray Antibody Arra 4-Well Tray Antibody Arra MOUSE ANTI BORRELIA BURGD succinate-CoA ligase, GDP formin-like 1 antibody So
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