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#27152507   2016/06/02 Save this To Up

Assessment of ADMA, estradiol, and progesterone in severe preeclampsia.

Decreased nitrous oxide (NO) levels are crucial factors in severe preeclampsia (sPE), and asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NO synthetase. Steroid hormones are closely related to the vascular endothelium. This study determined the levels of and correlations between ADMA, estradiol (E2), and progesterone (Pg) in sPE to investigate the roles of these factors in this disease.

2249 related Products with: Assessment of ADMA, estradiol, and progesterone in severe preeclampsia.

Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Progesterone Receptor (P Progesterone Receptor (P Progesterone Receptor (P Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser ING1B antisense ING1B sense Interferon γ

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#24555930   2014/02/21 Save this To Up

A direct competitive enzyme linked immunosorbent assay for progesterone using monoclonal antibody.

A direct competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody (MAb) was diagnosed with progesterone (P) level of human serum. In high concentrations and large amounts of displacer effect, this monoclonal antibody (MAb) can retain biological activity so that it can be specially combined with progesterone. Under conditions of the existing displaced agent, monoclonal antibody 11F8(3)H5 can maintain high specificity and affinity and can specifically bind progesterone in serum. Progesterone ELISA standard curve was calculated according to the following formula: Logit(y) = -1.358Log(x) + 0.4961, r = 0.9944. The serum progesterone values obtained by this method correlated well with those obtained by chemiluminescent immunoassay (CLIA): the correlative equation was y = 0.7804x + 0.7600, r = 0.9126.

1887 related Products with: A direct competitive enzyme linked immunosorbent assay for progesterone using monoclonal antibody.

MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD MOUSE ANTI CANINE DISTEMP MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI APAAP COMPLEX, Anti C Reactive Protein A Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon

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#23891914   2013/08/12 Save this To Up

Multiplexed analysis of steroid hormones in human serum using novel microflow tile technology and LC-MS/MS.

A novel microfluidic chromatography device coupled with tandem mass spectrometry (LC-MS/MS) was utilized for the multiplex analysis of 5 steroids (testosterone, dihydrotestosterone, progesterone, cortisol, cortisone) in human serum. The use of microfluidics allowed for reduction of the chromatographic flow rate to 3μl/min with overall method run times comparable to standard flow LC-MS/MS methods reported in the literature, corresponding to a 150 fold decrease in solvent consumption. Furthermore, a simple sample preparation protocol was employed requiring injection of only 0.5μl of sample, corresponding to a 100-400 fold increase in on-column sensitivity as compared to published standard flow assays. The measured LOQ for both testosterone and progesterone was 0.4ng/mL, representing an improvement over reported literature values obtained by standard flow methods employing comparable sample preparation and large injection volumes. The LOQs for cortisol (1.9ng/mL), cortisone (0.3ng/mL), and dihydrotestosterone (1.4ng/mL) were all within a biologically relevant range. A comparison of clinical serum samples was performed for the analysis of testosterone using this microfluidic LC-MS/MS assay and the Beckman Access II automated antibody-based measurement system. The immunoassay results were systematically higher due to matrix interference which was easily resolved with the increased chromatographic resolution obtained in the microflow LC-MS/MS assay.

2192 related Products with: Multiplexed analysis of steroid hormones in human serum using novel microflow tile technology and LC-MS/MS.

MS-275 (Entinostat) Mecha Human interleukin 2(IL-2) Anti Human IgG (H+L), Aff FITC conj.anti Human IgG Sterile filtered human se Sterile filtered human se Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon

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#23719105   2013/05/30 Save this To Up

[The influence of high fluoride exposure in drinking water on endocrine hormone in female].

To explore the influence of water fluoride exposure on reproductive hormones in female.

2943 related Products with: [The influence of high fluoride exposure in drinking water on endocrine hormone in female].

GLP 1 ELISA Kit, Rat Gluc Macrophage Colony Stimula Macrophage Colony Stimula Glucagon ELISA KIT, Rat G Leptin ELISA Kit, Rat Lep Anti beta3 AR Human, Poly Apoptosis antibody array Cell cycle antibody array Cytokine antibody array i Signal transduction antib AKT Phospho-Specific Arra AKT PKB Signaling Phospho

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#20107773   2010/09/30 Save this To Up

Measurement of progesterone in human serum by isotope dilution liquid chromatography-tandem mass spectrometry and comparison with the commercial chemiluminescence immunoassay.

Progesterone is one of the steroid hormones. The hormone is especially important in preparing the uterus for the implantation of the blastocyst and in maintaining pregnancy. Its concentration in serum is measured to determine ovarian function and to predict early pregnancy. The progesterone concentration is also important for in-vitro fertilization and embryo-transfer outcomes. We have established isotope dilution liquid chromatography-tandem mass spectrometry as a primary method for the measurement of progesterone in human serum. Progesterone and its isotopic analogue, progesterone-(13)C(2), in serum were monitored at mass transitions of m/z 315.2/109.2 and 317.2/111.2 respectively in multiple-reaction monitoring (MRM) mode with electrospray positive ionization. For validation of the method, progesterone in a National Institute of Standards and Technology standard reference material (NIST SRM) was measured, and the measured results were in good agreement with the reference values within the uncertainty. On the basis of the established method, progesterone certified reference material (CRM) was developed in this work. The certified value was (1.41 ± 0.036) μg kg(-1). The repeatability of 1.1% and reproducibility of 0.14% showed that ID LC-MS-MS is a reliable and reproducible method. The expanded uncertainty for the measurement of progesterone in the CRM was approximately 2.6% within 95% confidence limits. The detection limit of progesterone was approximately 0.6 μg kg(-1). The progesterone CRMs were distributed to representative clinical laboratories in the Republic of Korea for comparison with the chemiluminescence immunoassay (CLIA), which is the most sensitive immunoassay method. The results from the comparison showed quite a large bias among the participating laboratories. This implies that the CRM is a very important material for establishment of traceability to its practical use.

1896 related Products with: Measurement of progesterone in human serum by isotope dilution liquid chromatography-tandem mass spectrometry and comparison with the commercial chemiluminescence immunoassay.

Inflammation (Human) Quan Inflammation (Human) Quan Inflammation (Human) Quan CLIA Human Serum Progeste Progesterone CLIA Human S Human interleukin 2(IL-2) Leptin ELISA Kit, Human A Sterile filtered human se Sterile filtered human se Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s

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#17691581   2007/08/13 Save this To Up

[Effect of electroacununcture on sex hormone levels in patients with Sjögren's syndrome].

To observe the effect of electroacupuncture (EA) on serum testosterone (T), estradiol (F2), luteotropic hormone (LH), follicle-stimulating hormone (FSH), progesterone (P), and prolactin (PRL) in patients with Sjögren's Syndrome (SS) in order to analyze the correlation between the adjustment effect of EA and changes of hormone levels.

1285 related Products with: [Effect of electroacununcture on sex hormone levels in patients with Sjögren's syndrome].

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