Only in Titles

           Search results for: Caspase-10/b, human recombinant   

paperclip

#29055789   2017/10/22 Save this To Up

In vitro studies on the role of recombinant human soluble thrombomodulin in the context of retinoic acid mediated APL differentiation syndrome.

Recombinant human soluble thrombomodulin (rTM) is a newly developed anti-coagulant approved for treatment of disseminated intravascular coagulation (DIC) in Japan. rTM exerts anti-inflammatory and cytoprotective functions via its lectin-like and epidermal growth factor-like domains, respectively. In this study, we retrospectively reviewed the treatment of 21 consecutive patients with coagulopathy, complicated by acute promyelocytic leukemia (APL), with all-trans retinoic acid (ATRA) with or without combination with rTM. Surprisingly, none of the 14 rTM-treated patients developed retinoic acid (RA)-related differentiation syndrome (DS). The co-culture of vascular endothelial cell-derived EA.hy926 and APL-derived NB4 cells in the presence of RA increased production of tumor necrosis factor alpha (TNF-α) in culture media, in parallel with activation of p38 mitogen-activated protein kinase and increased levels of intracellular adhesion molecule 1 (ICAM1) in EA.hy926 cells. This was also associated with increased levels of the phosphorylated forms of VE-cadherin and enhanced vascular permeability of EA.hy926 monolayers. Importantly, addition of rTM to this co-culture system inhibited the RA-induced phosphorylation of p38 and VE-cadherin and decreased ICAM1 and vascular permeability in EA.hy926 cells, without a decrease inthe levels of TNF-α. Taken together, use of rTM may be a promising treatment strategy to prevent DS in APL patients who receive ATRA.

1516 related Products with: In vitro studies on the role of recombinant human soluble thrombomodulin in the context of retinoic acid mediated APL differentiation syndrome.

Macrophage Colony Stimula Macrophage Colony Stimula Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle

Related Pathways

paperclip

#29055620   2017/10/22 Save this To Up

Bioengineered AAV Capsids with Combined High Human Liver Transduction In Vivo and Unique Humoral Seroreactivity.

Existing recombinant adeno-associated virus (rAAV) serotypes for delivering in vivo gene therapy treatments for human liver diseases have not yielded combined high-level human hepatocyte transduction and favorable humoral neutralization properties in diverse patient groups. Yet, these combined properties are important for therapeutic efficacy. To bioengineer capsids that exhibit both unique seroreactivity profiles and functionally transduce human hepatocytes at therapeutically relevant levels, we performed multiplexed sequential directed evolution screens using diverse capsid libraries in both primary human hepatocytes in vivo and with pooled human sera from thousands of patients. AAV libraries were subjected to five rounds of in vivo selection in xenografted mice with human livers to isolate an enriched human-hepatotropic library that was then used as input for a sequential on-bead screen against pooled human immunoglobulins. Evolved variants were vectorized and validated against existing hepatotropic serotypes. Two of the evolved AAV serotypes, NP40 and NP59, exhibited dramatically improved functional human hepatocyte transduction in vivo in xenografted mice with human livers, along with favorable human seroreactivity profiles, compared with existing serotypes. These novel capsids represent enhanced vector delivery systems for future human liver gene therapy applications.

1653 related Products with: Bioengineered AAV Capsids with Combined High Human Liver Transduction In Vivo and Unique Humoral Seroreactivity.

Mouse Anti-Human Liver Ag Rabbit Anti-Human Androge Rabbit Anti-Human Androge Pyruvate Kinase(liver RBC Human Liver Ferritin Human Liver Ferritin Human Liver Ferritin alkaline phosphatase (liv Beta Amyloid (1 42) High Beta Amyloid (1 42) High Lipoproteins, Human Plasm Glucokinase, human liver,

Related Pathways

paperclip

#29055168   2017/10/21 Save this To Up

Long-Term Safety of Growth Hormone - A Combined Registry Analysis.

Preliminary data from the French cohort of the Safety and Appropriateness of Growth hormone treatments in Europe (SAGhE) study raised concerns regarding the safety of recombinant human GH, suggesting that GH may increase mortality and incidence of stroke in patients treated during childhood for GH deficiency or short stature. We evaluated published safety data, focusing on mortality, neoplasms, cerebrovascular events and diabetes across a number of large-scale pharmaceutical company GH registries.

1789 related Products with: Long-Term Safety of Growth Hormone - A Combined Registry Analysis.

1,1'-Dioctadecyl-3,3,3',3 Epidermal Growth Factor ( Epidermal Growth Factor ( Human Growth Hormone anti Human Growth Hormone anti Human Growth Hormone anti GH (Growth Hormone) Antib Mouse Anti-Growth Hormone Mouse anti human Growth H Mouse anti human Growth H Mouse Anti-Parathyroid Ho Mouse Anti-Adrenocorticot

Related Pathways

paperclip

#29055159   2017/10/21 Save this To Up

Genomics of Natural Populations: Evolutionary Forces that Establish and Maintain Gene Arrangements in Drosophila pseudoobscura.

The evolution of complex traits in heterogeneous environments may shape the order of genes within chromosomes. Drosophila pseudoobscura has a rich gene arrangement polymorphism that allows one to test evolutionary genetic hypotheses about how chromosomal inversions are established in populations. D. pseudoobscura has >30 gene arrangements on a single chromosome that were generated through a series of overlapping inversion mutations with > 10 inversions with appreciable frequencies and wide geographic distributions. This study analyzes the genomic sequences of 54 strains of Drosophila pseudoobscura that carry one of six different chromosomal arrangements to test whether (1) genetic drift, (2) hitchhiking with an adaptive allele, (3) direct effects of inversions to create gene disruptions caused by breakpoints, or (4) indirect effects of inversions in limiting the formation of recombinant gametes are responsible for the establishment of new gene arrangements. We found that the inversion events do not disrupt the structure of protein coding genes at the breakpoints. Population genetic analyses of 2,669 protein coding genes identified 277 outlier loci harboring elevated frequencies of arrangement-specific derived alleles. Significant linkage disequilibrium occurs among distant loci interspersed between regions with low levels of association indicating that distant allelic combinations are held together despite shared polymorphism among arrangements. Outlier genes showing evidence of genetic differentiation between arrangements are enriched for sensory perception and detoxification genes. The data presented here support the indirect effect of inversion hypothesis where chromosomal inversions are favored because they maintain linked associations among multi-locus allelic combinations among different arrangements. This article is protected by copyright. All rights reserved.

1887 related Products with: Genomics of Natural Populations: Evolutionary Forces that Establish and Maintain Gene Arrangements in Drosophila pseudoobscura.

CELLKINES Natural Human I DNA (cytosine 5) methyltr Human Epstein-Barr Virus Mouse Epstein-Barr Virus Rat TGF-beta-inducible ea Rat TGF-beta-inducible ea Homogenizer for 24 sample Homogenizer for 8 samples Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti MIC2 Gene Protein, CD99; MIC2 Gene Protein, CD99;

Related Pathways

paperclip

#29055152   2017/10/21 Save this To Up

Predominance of regorafenib over sorafenib: restoration of membrane-bound MICA in hepatocellular carcinoma cells.

The multi-kinase inhibitor regorafenib (REG) was recently demonstrated to be effective in patients with sorafenib (SOR)-resistant hepatocellular carcinoma (HCC). Interestingly, SOR is known to enhance the accumulation of membrane-bound MHC class I polypeptide-related sequence A (mMICA) in HCC cells, and to block production of soluble MICA (sMICA), an immunological decoy. In addition, MICA is associated with HCC in patients with chronic hepatitis C. We have now compared the impact of REG and SOR on MICA in HCC cells, as well as the immunotherapeutic implications thereof.

1614 related Products with: Predominance of regorafenib over sorafenib: restoration of membrane-bound MICA in hepatocellular carcinoma cells.

Human breast invasive duc Liver hepatocellular carc Liver cancer (hepatocellu Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Normal liver and hepatoce Octyl â D 1 thioglucopyr anti HSV (II) gB IgG1 (mo anti HCMV IE pp65 IgG1 (m anti HCMV gB IgG1 (monocl

Related Pathways

paperclip

#29054598   2017/10/21 Save this To Up

Phlebotomus papatasi yellow-related and apyrase salivary proteins are candidates for vaccination against human cutaneous leishmaniasis.

Nowadays, there is no available vaccine for human leishmaniasis. Animal experiments demonstrate that pre-exposure to sand fly saliva confers protection against leishmaniasis. Our preceding work in humans indicates that Phlebotomus (P) papatasi saliva, induce the production of IL-10 by CD8+ T lymphocytes. The neutralization of IL-10 enhanced the activation of a T cells CD4+ population producing IFN-γ. Herein, we used a biochemical and functional genomics approach to identify the sand fly salivary components that are responsible for the activation of the Th1 immune response in humans therefore constituting potential vaccine candidates against leishmaniasis. Fractionated Phlebotomus papatasi salivary extracts were first tested on T lymphocytes of immune donors. We confirmed that the CD4+ lymphocytes proliferate and produce IFN-γ in response to stimulation with the proteins of a molecular weight > 30 KDa. Peripheral blood mononuclear cells from immune donors were transfected with plasmids coding for the most abundant proteins from P. papatasi salivary gland cDNA library. Our result showed that the "yellow related proteins", PPTSP42 and PPTSP44, and "apyrase", PPTSP36, are the proteins responsible for the aforementioned cellular immune response and IFN-γ-production. Strikingly, PPTSP44 triggered the highest level of lymphocyte proliferation and IFN-γ production. Multiplex cytokine analysis confirmed the Th1-polarized response induced by these proteins. Importantly, recombinant PPTSP44 validated the results observed with the DNA plasmid, further supporting that PPTSP44 constitutes a promising vaccine candidate against human leishmaniasis.

1452 related Products with: Phlebotomus papatasi yellow-related and apyrase salivary proteins are candidates for vaccination against human cutaneous leishmaniasis.

Native Human Lactoferrin, Recombinant Human Androge Bone Morphogenetic Protei Growth Differentiation Fa Goat Anti-Human, Mouse AR Rabbit Anti-Human Androge Rabbit Anti-Human Androge Parathyroid Hormone Relat Parathyroid Hormone Relat RANK Ligand Soluble, Huma succinate-CoA ligase, GDP Myotubularin related prot

Related Pathways

paperclip

#29054095   2017/10/20 Save this To Up

Ginkgolide B and bilobalide ameliorate neural cell apoptosis in α-synuclein aggregates.

The accumulation of aggregated forms of the α-Synuclein (α-Syn) is associated with the pathogenesis of Parkinson's disease (PD), a chronic progressive neurodegenerative disorder. Extensive evidences have shown the promising effects of Ginkgo biloba consumption on motor activity after PD. However, the mechanisms underline the α-Syn-induced cell damage and whether ginkgolides exert neuroprotection against this injury are unclear. Here we showed that aggregated recombinant human α-Syn, but not α-Syn monomers, triggered cell injury in cultured human neuroblastoma cell line SH-SY5Y in an apoptosis way by using flow cytometry and western blot assay. Moreover, pre-treatment with the Ginkgolide B (GB) or Bilobalide (BB) protected SH-SY5Y cells against α-Syn-induced cell viability decreases, and reduced cell apoptosis after aggregated α-Syn stimulation. Together, we firstly find that aggregated α-Syn induced cell apoptosis and GB and BB may attenuate aggregated α-Syn-induced cell apoptosis, which gives us an insight into the novel therapy for PD in future.

1140 related Products with: Ginkgolide B and bilobalide ameliorate neural cell apoptosis in α-synuclein aggregates.

CELLKINES Natural Human I Cell Meter™ Phosphatidy Cell Meter™ Phosphatidy Cell Meter™ Caspase 3 7 Cell Meter™ Caspase 9 A Cell Meter™ Annexin V B Cell Meter™ Annexin V B Cell Meter™ Annexin V B Cell Meter™ Annexin V B Cell Meter™ Annexin V B Cell Meter™ Annexin V B Cell Meter™ Annexin V B

Related Pathways

  •  
  • No related Items
paperclip

#29053917   2017/10/20 Save this To Up

A Novel Bioconjugation Strategy Using Elevated Hydrostatic Pressure: Case Study for Site Specific PEGylation of rhCNTF.

In this paper, we reported a novel strategy for site specific PEGylation of proteins using elevated hydrostatic pressure. The process was similar to the conventional one except the reactor was under elevated hydrostatic pressure. The model protein was recombinant human ciliary neurotrophic factor (rhCNTF), and the reagent was mono-methoxy-polyethylene glycol-maleimide (mPEG-MAL). PEGylation with mPEG-40kDa-MAL at pH 7.0 under normal pressure for 5 hours achieved less than 5% yield. In comparison, when the pressure was elevated, the PEGylation yield was increased dramatically, reaching nearly 90% at 250 MPa. Furthermore, the following phenomena were observed: 1) high hydrostatic pressure PEGylation (HHPP) could operate at a low reactant ratio of 1:1.2 (rhCNTF to mPEG-MAL), while the conventional process needs a much higher ratio. 2) short and long chains of PEG gave a similar yield of 90% in HHPP, while the conventional yield for the short chain of the PEG was higher than that of the long chain. 3) the reaction pH in the range of 7.0 to 8.0 had almost no influence upon the yield of HHPP, while the PEGylation yield was significantly increased by three times from pH 7.0 to pH 8.0 at normal pressure. Surface accessibility analysis was performed using GRASP2 software and found that Cys17 of rhCNTF was located at the concave patches, which may have steric hindrance for the PEG to approach. The speculated benefit of HHPP was facilitation of target site exposure, reducing the steric hindrance and making the reaction much easier. Structure and activity analysis demonstrated the HHPP product was comparable to the PEGylated rhCNTF prepared through conventional method. Overall, this work demonstrated that HHPP, as we proposed, may have application potentials in various conjugations of biomacromolecules.

1216 related Products with: A Novel Bioconjugation Strategy Using Elevated Hydrostatic Pressure: Case Study for Site Specific PEGylation of rhCNTF.

MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD NATIVE HUMAN PROLACTIN, P RABBIT ANTI GSK3 BETA (pS Mouse(FBV strain) normal Mouse(KM strain) normal t Mouse normal tissue array Normal stomach tissue (mu Normal stomach tissue (mu Normal liver tissue array Normal lung tissue (multi Normal colon tissue (mult

Related Pathways

paperclip

#29053450   2017/10/20 Save this To Up

Two-Dimensional Isoelectric Focusing OFFGEL, Micro-fluidic Lab-on-Chip Electrophoresis and FTIR for assessment of long-term stability of rhG-CSF formulation.

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) has been increasingly recognized from among one of the most abundant families of biosimilars. Upon long-term storage, rhG-CSF is subject to subtle chemical modifications that rapidly occur and, in particular, produce deaminated variants with divergent charge. Indeed, changes in charge from glutamine deamination may alter the way rhG-SCF will refold and the structure of resulting molecule. To assess this charge heterogeneity, 2D gel electrophoresis has limited application. Recent micro-fluidic based technical advances offer a great alternative method to better control liquid volumes on a minute scale. Here, we used IEF OFFGEL-Lab-On-Chip electrophoresis for two-dimensional separation of rhG-CSF peptides according to their isoelectric point (pI) and molecular weight (kDa). We used a rhG-CSF commercial therapeutic formulation, kept refrigerated 24 months after expiry. The samples were analyzed for particulate matter and charge variants. Subsequently, the secondary structure was assessed by FTIR spectroscopy and residual biological activity was recorded. Interestingly, we showed an additional band in the acidic gel area above and below the most intense protein band (fractions 10, 11 and 12 at 22.84s). This observation reveals the presence of rhG-CSF variant charges without any additional high molecular weight impurity or biological activity decrease. We conclude that after two years of storage, the rhG-CSF solution maintained its native secondary structure with little β-sheet deviation, as reflected in the 1622 cm-1 and 1695 cm-1. These data demonstrated that a combined strategy is a more suitable and accurate analytical assessment of rhG-CSF and recombinant protein-based biosimilars.Recombinant human granulocyte colony-stimulating factor (rhG-CSF) has been increasingly recognized from among one of the most abundant families of biosimilars. Upon long-term storage, rhG-CSF is subject to subtle chemical modifications that rapidly occur and, in particular, produce deaminated variants with divergent charge. Indeed, changes in charge from glutamine deamination may alter the way rhG-SCF will refold and the structure of resulting molecule. To assess this charge heterogeneity, 2D gel electrophoresis has limited application. Recent micro-fluidic based technical advances offer a great alternative method to better control liquid volumes on a minute scale. Here, we used IEF OFFGEL-Lab-On-Chip electrophoresis for two-dimensional separation of rhG-CSF peptides according to their isoelectric point (pI) and molecular weight (kDa). We used a rhG-CSF commercial therapeutic formulation, kept refrigerated 24 months after expiry. The samples were analyzed for particulate matter and charge variants. Subsequently, the secondary structure was assessed by FTIR spectroscopy and residual biological activity was recorded. Interestingly, we showed an additional band in the acidic gel area above and below the most intense protein band (fractions 10, 11 and 12 at 22.84s). This observation reveals the presence of rhG-CSF variant charges without any additional high molecular weight impurity or biological activity decrease. We conclude that after two years of storage, the rhG-CSF solution maintained its native secondary structure with little β-sheet deviation, as reflected in the 1622 cm-1 and 1695 cm-1. These data demonstrated that a combined strategy is a more suitable and accurate analytical assessment of rhG-CSF and recombinant protein-based biosimilars.

1629 related Products with: Two-Dimensional Isoelectric Focusing OFFGEL, Micro-fluidic Lab-on-Chip Electrophoresis and FTIR for assessment of long-term stability of rhG-CSF formulation.

Primary antibody FLIP An Isopeptidase T (long form Goat Anti-Human Androgen Goat Anti-Human STUB1 CHI 5 Carboxy X Rhodamine, NH 1,1'-Dioctadecyl-3,3,3',3 Ofloxacin CAS Number [824 Anti- ADAM-12 (A Disintig Anti ADAM 12 (A Disintigr Anti-ADAMTS-13 (A Disinti Anti-BMP-1 (Bone Morphoge Interleukin-34 IL34 (N-t

Related Pathways

paperclip

#29052939   2017/10/20 Save this To Up

QTL mapping and analysis of heritable variation in affiliative social behavior and related traits.

Humans exhibit broad heterogeneity in affiliative social behavior. Twin and family studies demonstrate that individual differences in core dimensions of social behavior are heritable, yet there are knowledge gaps in understanding the underlying genetic and neurobiological mechanisms. Animal genetic reference panels (GRPs) provide a tractable strategy for examining the behavioral and genetic architecture of complex traits. Here, using males from 50 mouse strains from the BXD GRP, four domains of affiliative social behavior-social approach, social recognition, direct social interaction (partner sniffing), and vocal communication-were examined in two widely used behavioral tasks-the 3-chamber and direct social interaction tasks. There was continuous and broad variation in social and non-social traits, with moderate to high heritability of social approach sniff preference (0.31), ultrasonic vocalization (USV) count (0.39), partner sniffing (0.51), locomotor activity (0.54-0.66), and anxiety-like behavior (0.36). Principal component analysis reveals that variation in social and non-social traits are attributable to 5 independent factors. Genome-wide mapping identified significant quantitative trait loci for USV count on chromosome (Chr) 18 and locomotor activity on Chr X, with suggestive loci and candidate quantitative trait genes identified for all traits with one notable exception-partner sniffing in the direct social interaction task. The results demonstrate heritable variation in sociability, which is independent of variation in activity and anxiety-like traits. In addition, a highly heritable and ethological domain of affiliative sociability-partner sniffing-appears highly polygenic. These findings establish a basis for identifying functional natural variants, leading to a new understanding typical and atypical sociability.

1507 related Products with: QTL mapping and analysis of heritable variation in affiliative social behavior and related traits.

Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Androgen Receptor (Ab 650 AZD-3514 Mechanisms: Andr 17β-Acetoxy-2α-bromo-5 (5α,16β)-N-Acetyl-16-[2 (5α,16β)-N-Acetyl-16-ac 5α-N-Acetyl-2'H-androst- 5α-N-Acetyl-2'H-androst- 3-O-Acetyl 5,14-Androstad

Related Pathways