Search results for: Caspase 12 Fluorometric Assay Kit
#30417434 2018/11/11 To Up
Adenosine protects pancreatic beta cells against apoptosis induced by endoplasmic reticulum stress.
Chronic exposure to high glucose induces endoplasmic reticulum (ER) stress in pancreatic beta cells (PBCs). The previous evidence showed that adenosine modulate PBCs viability and insulin secretion. The aim of this study was to evaluate possible involvement of adenosine in protection of MIN6 β-cells from Tunicamycin (Tu)-induced ER stress. MIN6 cells were cotreated with Tu and different concentrations of adenosine. Cell viability, proliferation, and apoptosis were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT), 5-bromo-2'-deoxyuridine (Brdu), and colony formation assays. Caspase-12 activity was assayed using the fluorometric method. Thioflavin T (ThT) staining was used for the evaluation of protein aggregation. Insulin secretion was evaluated using specific an ELISA kit. Ca mobilization assayed using Fura2/AM probe. BIP, CHOP, XBP-1, and XBP-1s expression in both messenger RNA (mRNA) and protein levels were evaluated using the reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Bcl-2, p-eIF2α/eIF2α, and GADD34 levels also determined with Western blot analysis. Adenosine protected MIN6 cells against Tu-induced ER stress in a dose-dependent manner and increased their proliferation. Decreased caspase-12 activity and upregulated Bcl-2 protein may explain antiapoptotic effects of adenosine. ThT staining indicated an attenuated aggregation of misfolded proteins. Adenosine effectively increased insulin secretion in Tu-treated cells. BIP, CHOP, XBP1, and sXBP1 expression were decreased significantly in cotreated cells, indicating alleviation of ER stress. However, adenosine potentiated the expression of GADD34 and decreased p-eIF2α/eIF2α ratio. Adenosine increased cytosolic Ca levels, which may promote adenosine triphosphate (ATP) synthesis in mitochondria, helping ER to preserve protein hemostasis. Taken together, adenosine upregulated Bcl-2 and GADD34 to protect PBCs against Tu-induced apoptosis and increase Insulin secretion.Mohammad Keyvanloo Shahrestanaki, Fatemeh Panahi Arasi, Mahmoud Aghaei
1235 related Products with: Adenosine protects pancreatic beta cells against apoptosis induced by endoplasmic reticulum stress.
100ul250 0.25 mL96 tests1.00 flask 100ul 100 UG100ug100ug100ug LyophilizedRelated Pathways
#26775664 2016/01/18 To Up
Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress.
Sulforaphane (SFN), a natural dietary isothiocyanate, is found to exert beneficial effects for cardiovascular diseases. This study aimed to investigate the mechanisms underlying the protective effects of SFN in a model of myocardial hypoxia/reoxygenation (H/R) injury in vitro.Yun-peng Li, Shu-lin Wang, Bei Liu, Lu Tang, Rong-ren Kuang, Xian-bao Wang, Cong Zhao, Xu-dong Song, Xue-ming Cao, Xiang Wu, Ping-zhen Yang, Li-zi Wang, Ai-hua Chen
2290 related Products with: Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress.
100 UG1 Set50 ug1mg1 Set1 Set1 Set10mg100 μg1 Set1 Set100 μgRelated Pathways
#22963151 // To Up
Involvement of endoplasmic reticulum stress in isoliquiritigenin-induced SKOV-3 cell apoptosis.
IXuan Yuan, Bacui Yu, Yanming Wang, Jiangtao Jiang, Liangliang Liu, Hong Zhao, Wang Qi, Qiusheng Zheng
1236 related Products with: Involvement of endoplasmic reticulum stress in isoliquiritigenin-induced SKOV-3 cell apoptosis.
96 assays400 ug 100ul400 ug1 mg1 mg25 g500 ml5 x 2 ml1 kit30mlRelated Pathways
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#21473903 2011/04/05 To Up
Apoptogenic activity of 2α,3α-dihydroxyurs-12-ene-28-oic acid from Prunella vulgaris var. lilacina is mediated via mitochondria-dependent activation of caspase cascade regulated by Bcl-2 in human acute leukemia Jurkat T cells.
The dried spikes of Prunella vulgaris var. lilacina (Labiatae) have been used for traditional herbal medicine to treat fever, inflammation, dropsy, gonorrhea and cancer in Korea, Japan and China. The present study evaluated the apoptotic effect of 2α,3α-dihydroxyurs-12-en-28-oic acid (DHURS), purified from the dried spikes on human acute leukemia Jurkat T cells.Hyun Ju Woo, Do Youn Jun, Ji Young Lee, Mi Hee Woo, Chae Ha Yang, Young Ho Kim
2039 related Products with: Apoptogenic activity of 2α,3α-dihydroxyurs-12-ene-28-oic acid from Prunella vulgaris var. lilacina is mediated via mitochondria-dependent activation of caspase cascade regulated by Bcl-2 in human acute leukemia Jurkat T cells.
0.1ml (1mg/ml)100ug Lyophilized100ug Lyophilized25mg100ug Lyophilized 1 G96T100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug LyophilizedRelated Pathways
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