Only in Titles

           Search results for: Caspase 8 Fluorometric Assay Kit   

paperclip

#26141531   2015/08/28 Save this To Up

Analysis of porcine granulosa cell death signaling pathways induced by vinclozolin.

Recent studies suggest that disturbing androgen-signaling pathways in porcine ovarian follicles may cause granulosa cell (GC) death. For this reason, we investigated which apoptotic pathway is initiated after GC exposure to an environmental antiandrogen, vinclozolin (Vnz), in vitro. Immunocytochemistry, Western blots, and fluorometric assays were used to quantify caspase-3 and -9 expression and activity. To elucidate the specific mechanism of Vnz action and toxicity, GCs were assessed for viability, cytotoxicity, and apoptotic activity using the ApoTox-Glo Triplex Assay. To further determine the mechanism of GC death induced by Vnz, we used the Apoptosis Antibody Array Kit. In response to Vnz stimulus, we found an increased level of caspase-3 protein expression (P ≤ 0.001) and an increase in caspase-3 proteolytic activity (P ≤ 0.001), confirming that Vnz is a potent proapoptotic factor. The strong immunoreaction of caspase-9 after Vnz treatment (P ≤ 0.001) suggests that intrinsic mitochondrial apoptosis pathway was activated during GC death. On the other hand, caspase-8, being a part of the extrinsic receptor pathway, was also activated (P ≤ 0.001). Therefore, it is possible that Vnz induces porcine granulosal apoptosis also through a parallel pathway. Activation of these two pathways was confirmed by the Apoptosis Antibody Array Kit. In conclusion, it is possible that the intrinsic signaling pathway may not act as an initial trigger for GC apoptosis but might contribute to the amplification and propagation of apoptotic cell death in the granulosa layer after treatment with this antiandrogen. Moreover, Vnz disturbs the physiological process of programmed cell death. Consequently, this could explain why atretic follicles are rapidly removed and suggests that normal function of the ovarian follicle may be destroyed.

1700 related Products with: Analysis of porcine granulosa cell death signaling pathways induced by vinclozolin.

T-cell proliferation grad TCPI T cell proliferation TCPI T cell proliferation Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu T-cell proliferation grad TCPII T cell proliferatio TCPII T cell proliferatio AP-1 Reporter – HEK293 Wnt Signaling Pathway TCF

Related Pathways

paperclip

#21473903   2011/05/23 Save this To Up

Apoptogenic activity of 2α,3α-dihydroxyurs-12-ene-28-oic acid from Prunella vulgaris var. lilacina is mediated via mitochondria-dependent activation of caspase cascade regulated by Bcl-2 in human acute leukemia Jurkat T cells.

The dried spikes of Prunella vulgaris var. lilacina (Labiatae) have been used for traditional herbal medicine to treat fever, inflammation, dropsy, gonorrhea and cancer in Korea, Japan and China. The present study evaluated the apoptotic effect of 2α,3α-dihydroxyurs-12-en-28-oic acid (DHURS), purified from the dried spikes on human acute leukemia Jurkat T cells.

1405 related Products with: Apoptogenic activity of 2α,3α-dihydroxyurs-12-ene-28-oic acid from Prunella vulgaris var. lilacina is mediated via mitochondria-dependent activation of caspase cascade regulated by Bcl-2 in human acute leukemia Jurkat T cells.

Human normal bone and ost Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-TNIP2 ABIN2 T Multiple tumor and normal Caspase-12 Inhibitor Z-AT Caspase-12 Inhibitor Z-AT Caspase 12 Inhibitor 20 u Caspase 12 Inhibitor Z AT Cell Meter™ Caspase 9 A Recombinant Human Interfe Recombinant Human Interle Recombinant Human Interle

Related Pathways

paperclip

#19159073   2009/01/22 Save this To Up

Extracellular adenosine induces apoptosis in Caco-2 human colonic cancer cells by activating caspase-9/-3 via A(2a) adenosine receptors.

Extracellular adenosine has been shown to induce apoptosis in a variety of cells via an intrinsic pathway linked to adenosine uptake into cells and the ensuing signaling cascades and an extrinsic pathway linked to adenosine receptors. The present study was designed to understand the mechanism underlying adenosine-induced apoptosis of Caco-2 human colonic cancer cells.

1168 related Products with: Extracellular adenosine induces apoptosis in Caco-2 human colonic cancer cells by activating caspase-9/-3 via A(2a) adenosine receptors.

Human Interleukin-33 IL-3 Human Interleukin-32 alph Active Human Caspase 325 Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe 8-Amino Adenosine C10H14N N6-Benzyl-5’-ethylcarbo Rabbit Anti-Apoptosis enh Rabbit Anti-Apoptosis enh Goat Anti-Human GOT1 (aa

Related Pathways

paperclip

#16539849   2006/03/16 Save this To Up

Molecular mechanisms of adenosine-induced apoptosis in human HepG2 cells.

To investigate effects of adenosine on cell proliferation and apoptosis in human HepG2 cells.

2037 related Products with: Molecular mechanisms of adenosine-induced apoptosis in human HepG2 cells.

Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Human Epstein-Barr Virus Macrophage Colony Stimula Macrophage Colony Stimula TGF beta induced factor 2 Apoptosis (Human) Antibod Apoptosis (Human) Antibod Human Small Intestine Mic Human Large Intestine Mic Human Internal Mammary Ar GFP Expressing Human Inte

Related Pathways