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Search results for: Caspase 9 Substrate LEHD AFC1000 assays

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#29579407   2018/11/07 To Up

Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway.

Ketamine is a widely used anesthetic in pediatric clinical practice. Previous studies have demonstrated that ketamine induces neurotoxicity and has a modulatory effect on the cells of the immune system. Here, we evaluated the potential protective effect and underlying mechanisms of natural phenolic compound curcumin against ketamine-induced toxicity in rat thymocytes. Rat thymocytes were exposed to 100 µM ketamine alone or combined with increasing concentrations of curcumin (0.3, 1, and 3 μM) for 24 hours. Cell viability was analyzed with CCK-8 assay kit. Apoptosis was analyzed using flow cytometry and propidium iodide as well as Z-VAD-FMK and Z-LEHD-FMK inhibitors. Reactive oxygen species (ROS) production and mitochondrial membrane potential [MMP] were measured by flow cytometry. Colorimetric assay with DEVD-pNA substrate was used for assessing caspase-3 activity. Involvement of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was tested with Wortmannin inhibitor. Ketamine induced toxicity in cells, increased the number of hypodiploid cells, caspase-3 activity and ROS production, and inhibited the MMP. Co-incubation of higher concentrations of curcumin (1 and 3 μM) with ketamine markedly decreased cytotoxicity, apoptosis rate, caspase-3 activity, and ROS production in rat thymocytes, and increased the MMP. Application of Z-VAD-FMK (a pan caspase inhibitor) or Z-LEHD-FMK (caspase-9 inhibitor) with ketamine effectively attenuated the ketamine-induced apoptosis in rat thymocytes. Administration of Wortmannin (a PI3K inhibitor) with curcumin and ketamine significantly decreased the protective effect of curcumin on rat thymocytes. Our results indicate that ketamine-induced toxicity in rat thymocytes mainly occurs through the mitochondria-mediated apoptotic pathway and that the PI3K/Akt signaling pathway is involved in the anti-apoptotic effect of curcumin.
Svetlana Pavlovic, Zorica Jovic, Radmila Karan, Dane Krtinic, Gorana Rankovic, Mladjan Golubovic, Jelena Lilic, Voja Pavlovic

1780 related Products with: Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway.

96T100 μg 100ul100ug100ug100ug 100ul96T1 mg1 Set96T

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#27748905   2016/10/13 To Up

Resveratrol analogue (E)-8-acetoxy-2-[2-(3,4-diacetoxyphenyl)ethenyl]-quinazoline induces apoptosis via Fas-mediated pathway in HL-60 human leukemia cells.

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Eun Young Park, Joo-Il Kim, Dong-Gyu Leem, Ji-Sun Shin, Kyung-Tack Kim, Sang Yoon Choi, Myung-Hee Lee, Jung-Hye Choi, Yong Sup Lee, Kyung-Tae Lee

2180 related Products with: Resveratrol analogue (E)-8-acetoxy-2-[2-(3,4-diacetoxyphenyl)ethenyl]-quinazoline induces apoptosis via Fas-mediated pathway in HL-60 human leukemia cells.

100 μg2 Pieces/Box4 Membranes/Box1.00 flask100 ug/vial1.00 flask100 100 ug/vial4 Arrays/Slide2 Pieces/Box1.00 flask1 mg

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#26407943   2015/07/10 To Up

The abietane diterpenoid parvifloron D from Plectranthus ecklonii is a potent apoptotic inducer in human leukemia cells.

Abietane diterpenes have attracted much attention because they display a wide range of biological activities, including antitumor activities. These compounds are the most diverse of the diterpenoids isolated from species of Plectranthus. Naturally occurring diterpene parvifloron D is the main phytochemical constituent of Plectranthus ecklonii. To examine the therapeutic potential of the plant, we evaluated whether parvifloron D displays cytotoxicity against human tumor cells.
Olga Burmistrova, Juan Perdomo, M Fátima Simões, Patricia Rijo, José Quintana, Francisco Estévez

2695 related Products with: The abietane diterpenoid parvifloron D from Plectranthus ecklonii is a potent apoptotic inducer in human leukemia cells.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100 μg96 tests100ug Lyophilized100 μg100ug Lyophilized100ug Lyophilized100ug Lyophilized50 ug

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#25773833   // To Up

Enterocarpam-III induces human liver and breast cancer cell apoptosis via mitochondrial and caspase-9 activation.

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Ratana Banjerdpongchai, Benjawan Wudtiwai, Wilart Pompimon

2317 related Products with: Enterocarpam-III induces human liver and breast cancer cell apoptosis via mitochondrial and caspase-9 activation.

100 ug/vial1.00 flask96tests100 ug/vial96tests100 Tests1 kit1 kit(96 Wells)96tests1 kit100 Tests200 Tests

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#21296132   2011/02/04 To Up

T-2 toxin induces apoptosis in ovarian granulosa cells of rats through reactive oxygen species-mediated mitochondrial pathway.

To investigate the reproductive toxicity and cytotoxicity of T-2 toxin, which is a mycotoxin, and to explore its potential apoptotic induction mechanism.
Jing Wu, Li Jing, Hui Yuan, Shuang-qing Peng

2729 related Products with: T-2 toxin induces apoptosis in ovarian granulosa cells of rats through reactive oxygen species-mediated mitochondrial pathway.

2 Pieces/Box2 Pieces/Box1x10e7 cells96T20ug10mg 100ul 500 G

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#20162409   2010/02/17 To Up

Magnolol induces apoptosis via activation of both mitochondrial and death receptor pathways in A375-S2 cells.

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Qingjun You, Mingqiu Li, Guoqing Jiao

1969 related Products with: Magnolol induces apoptosis via activation of both mitochondrial and death receptor pathways in A375-S2 cells.

100 ug/vial2 Pieces/Box100ug100 μg100ug Lyophilized2 Pieces/Box100ug100ug Lyophilized200ul100ug100ug

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#17658284   2007/06/26 To Up

Specific inhibition of caspase-8 and -9 in CHO cells enhances cell viability in batch and fed-batch cultures.

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Chee Yong Yun, Sen Liu, Sing Fee Lim, Tianhua Wang, Beatrice Y F Chung, Joong Jiat Teo, Kok Hwee Chuan, Allyson S C Soon, Keng Siong Goh, Zhiwei Song

2455 related Products with: Specific inhibition of caspase-8 and -9 in CHO cells enhances cell viability in batch and fed-batch cultures.

1.00 flask96 tests10 ug1 mg1x10e7 cells1.00 flask-96 wells1x10e7 cells1 mg

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#17574045   // To Up

The essential role of the mitochondria and reactive oxygen species in Cisplatin-mediated enhancement of fas ligand-induced apoptosis in malignant pleural mesothelioma.

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John H Stewart, Thai-Lan Tran, Nicole Levi, Wilson S Tsai, David S Schrump, Dao M Nguyen

2398 related Products with: The essential role of the mitochondria and reactive oxygen species in Cisplatin-mediated enhancement of fas ligand-induced apoptosis in malignant pleural mesothelioma.

1 100ul96T 100ul

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#17112418   // To Up

Involvement of mitochondria and caspase pathways in N-demethyl-clarithromycin-induced apoptosis in human cervical cancer HeLa cell.

To study the mechanisms by which N-demethyl-clarithromycin (NDC) induces human cervical cancer HeLa cell apoptosis in vitro.
Ai-min Qiao, Takashi Ikejima, Shin-ichi Tashiro, Satoshi Onodera, Wei-ge Zhang, Ying-liang Wu

2621 related Products with: Involvement of mitochondria and caspase pathways in N-demethyl-clarithromycin-induced apoptosis in human cervical cancer HeLa cell.

2 Pieces/Box400 ug100 1 mg5ug50 ul

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#16796750   2006/06/23 To Up

TUCAN/CARDINAL/CARD8 and apoptosis resistance in non-small cell lung cancer cells.

Activation of caspase-9 in response to treatment with cytotoxic drugs is inhibited in NSCLC cells, which may contribute to the clinical resistance to chemotherapy shown in this type of tumor. The aim of the present study was to investigate the mechanism of caspase-9 inhibition, with a focus on a possible role of TUCAN as caspase-9 inhibitor and a determinant of chemosensitivity in NSCLC cells.
Agnieszka Checinska, Giuseppe Giaccone, Bas S J Hoogeland, Carlos G Ferreira, Jose A Rodriguez, Frank A E Kruyt

1627 related Products with: TUCAN/CARDINAL/CARD8 and apoptosis resistance in non-small cell lung cancer cells.

1.00 flask

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