Search results for: Citrate Assay Kit
#28738537 2017/07/25 Save this To Up
Phosphodiesterase inhibitor ameliorates neuronal injury in spinal cord ischemia/reperfusion injured rat model.This study investigated the mechanisms responsible for the neuroprotective effect of sildenafil citrate (SFC) on ischemia-reperfusion spinal cord (SC) injuries. Balloon occlusion of the thoracic aorta was used to induce SC ischemia. The animals (n=30) were separated into three groups: sham, SC injury with saline, and SC injury with 5mg/kg i.p. SFC treatment (SFC). The Basso, Beattie, and Bresnahan (BBB) score was determined to assess neurological function at different time intervals after reperfusion. After 48h, histopathology of the SC was assessed by triphenyltetrazolium chloride (TTC) and Nissl staining. Myeloperoxidase (MPO) activity was estimated using an MPO assay kit. Western blot and ELISA assays were performed to estimate interleukin 1 & 10 (IL-1 & IL-10), tumour necrosis factor α (TNF-α), and nuclear factor (NF-kB) levels in SC tissue homogenates. The study results suggest that treatment with SFC significantly increased neurological function compared with the SC group. In addition, SFC treatment reduced MPO activity compared with the SC group, which subsequently inhibited the infiltration of neutrophils into the SC. There was a significant (p<0.01) decrease in the expression of IL-1 and TNF-α, and an increase in the expression of IL-10 in SFC tissue homogenates compared with SC tissues. Moreover, SFC treatment inhibited the activation of NF-kB in the SC after injury. This study shows that SFC exerts a neuroprotective effect on the SC after ischemia/reperfusion injury by attenuating inflammatory mediators.
1755 related Products with: Phosphodiesterase inhibitor ameliorates neuronal injury in spinal cord ischemia/reperfusion injured rat model.Rat Visceral adipose spec p130Cas-associated protei Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Sterile filtered rat ser Anti VGLUT 1 Rat, polyclo Anti Rat VGLUT 2, Rabbit Insulin 1 (Rat), syntheti Human Migration Inhibitor Caspase-3 Inhibitor Z-DEV
#28665969 2017/06/30 Save this To Up
Potentiated virucidal activity of pomegranate rind extract (PRE) and punicalagin against Herpes simplex virus (HSV) when co-administered with zinc (II) ions, and antiviral activity of PRE against HSV and aciclovir-resistant HSV.There is a clinical need for new therapeutic products against Herpes simplex virus (HSV). The pomegranate, fruit of the tree Punica granatum L, has since ancient times been linked to activity against infection. This work probed the activity of pomegranate rind extract (PRE) and co-administered zinc (II) ions.
1211 related Products with: Potentiated virucidal activity of pomegranate rind extract (PRE) and punicalagin against Herpes simplex virus (HSV) when co-administered with zinc (II) ions, and antiviral activity of PRE against HSV and aciclovir-resistant HSV.Goat Anti-Herpes Simplex Goat Anti-Herpes Simplex Goat Anti-Herpes Simplex Goat Anti-Herpes Simplex Mouse Anti-Herpes Simplex Herpes Simplex Virus 1 (H West Nile Virus Pre M rec Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Androgen Receptor (Ab 650
#28412214 2017/04/16 Save this To Up
Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor β1 signaling pathway in vitro and in vivo.Traditional Chinese medicine Bu-Fei decoction (BFD) has been utilized to treat patients with Qi deficiency for decades, with the advantages of invigorating vital energy, clearing heat-toxin and moistening lung, etc. According to previous clinical experience and trials, BFD has been found to indeed improve life quality of lung cancer patients and prolong survival time. Nevertheless, little is known on its potential mechanisms so far. Being regarded as a pivotal cytokine in the tumor microenvironment, transforming growth factor β (TGF-β) stands out as a robust regulator of epithelial-mesenchymal transition (EMT), which is closely linked to tumor progression.
1795 related Products with: Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor β1 signaling pathway in vitro and in vivo.IGF-1R Signaling Phospho- Lung non small cell cance Non-small cell lung cance T-Cell Receptor Signaling Small cell lung carcinoma Non small cell lung carci Non small cell lung carci Lung small cell carcinoma High density non small ce Middle advanced stage lun Multiple lung carcinoma ( Human Insulin-like Growth
#28271822 2017/03/08 Save this To Up
Crystal Diagnostics Xpress S Kit for the Rapid Detection of Salmonella spp. in Selected Food Matrixes.The Crystal Diagnostics (CDx) Xpress S Kit is a rapid-screening assay for Salmonella spp. in whole raw tomatoes, whole chicken carcasses, raw ground beef, raw beef trim, and whole liquid pasteurized eggs with citric acid when present at levels of 1 CFU/portion size. The Xpress S system comprises an automatic CDx Xpress Reader and a single-use CDx BioCassette that incorporates antibody-coupled microspheres and liquid crystal for the selective identification of the intended microbe. In internal evaluations, the CDx Xpress S Kit detected all 142 Salmonella strains tested, including non-enterica subspecies, and excluded all non-Salmonella species assayed. Method-developer studies, as well as a third-party evaluation, demonstrated that 15 h single-stage enrichment permits rapid detection equivalent to the U.S. Department of Agriculture and U.S. Food and Drug Administration reference methods. The results demonstrate that the CDx Xpress S Kit is one of the fastest, most sensitive, and most accurate methods for detecting Salmonella in food matrixes.
1586 related Products with: Crystal Diagnostics Xpress S Kit for the Rapid Detection of Salmonella spp. in Selected Food Matrixes.EnzyChrom™ Kinase Assay MarkerGeneTM in vivo lacZ H. Pylori Rapid Stain Ki H. Pylori Rapid Stain Ki Casein ELISA Kit Milk ca Aurora Kinase B Inhibitor Aurora Kinase B Inhibitor SCD1 Inhibitor; Appearanc SCD1 Inhibitor; Appearanc EtBr Destaining Bag Kit A Beta Amyloid (42) ELISA K GLP 1 ELISA Kit, Rat Gluc
#28258955 2017/03/04 Save this To Up
Inhibition of Cyclic GMP Export by Multidrug Resistance Protein 4: A New Strategy to Treat Erectile Dysfunction?Intracellular cyclic guanosine monophosphate (cGMP) concentrations are regulated by degradation enzymes (phosphodiesterases) and by active transport across the plasma membrane by multidrug resistance proteins (MRPs) 4 and 5.
2245 related Products with: Inhibition of Cyclic GMP Export by Multidrug Resistance Protein 4: A New Strategy to Treat Erectile Dysfunction?TOM1-like protein 2 antib Recombinant Human IFN-alp Recombinant Human IFN-alp Cyclic AMP Total EIA Kit Cyclic AMP Total EIA Kit MarkerGene™ Total Prote Rabbit Anti-Human Toll In Human Dnak (HSP70) His ta Toxoplasma gondii GRA8, r FIV Core Ag, recombinant Rat Tissue Lysate (total Anti-ARS2(Arsenite-resist
#28210651 2017/02/17 Save this To Up
Analysis of Platelet-Rich Plasma Extraction: Variations in Platelet and Blood Components Between 4 Common Commercial Kits.Platelet-rich plasma (PRP) has been extensively used as a treatment in tissue healing in tendinopathy, muscle injury, and osteoarthritis. However, there is variation in methods of extraction, and this produces different types of PRP.
1964 related Products with: Analysis of Platelet-Rich Plasma Extraction: Variations in Platelet and Blood Components Between 4 Common Commercial Kits.Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon CELLKINES PLATELET DERIVE PLATELET DERIVED GROWTH F CELLKINES PLATELET DERIVE PLATELET DERIVED GROWTH F Human Platelet Derived Gr Human Platelet Derived Gr Human Platelet Derived Gr Jurkat Cell Extract (Indu
#28042534 2017/01/02 Save this To Up
Effect of Food Additive Citric Acid on The Growth of Human Esophageal Carcinoma Cell Line EC109.Today, esophageal cancer (EC) has become one of the most common cancer types in China. Therefore, new drug and therapeutic strategies are urgently needed to improve postoperative survival rate of patients with EC. As a food additive, several lines of evidence have shown that citric acid can be served as glycolysis suppressor to inhibit growth of some tumor cells. However, little is known about the effect of this organic acid on the growth of human esophageal carcinoma cell line, EC109.
2924 related Products with: Effect of Food Additive Citric Acid on The Growth of Human Esophageal Carcinoma Cell Line EC109.Epidermal Growth Factor ( Epidermal Growth Factor ( Fibroblast Growth Factor Fibroblast Growth Factor anti SLAM anti CDw150 IgG Growth Differentiation Fa Human Fibroblast Growth F RABBIT ANTI HUMAN SDF-1 A Human tumor cell array, 1 Human tumor cell array, 1 Human Squamous Cell Carci Human squamous cell carci
#27837855 2016/11/13 Save this To Up
Natural deep eutectic solvents in combination with ultrasonic energy as a green approach for solubilisation of proteins: application to gluten determination by immunoassay.In this work, a fast and miniaturised procedure based on the use of a natural deep eutectic solvent (NADES) in combination with ultrasound-assisted extraction (UAE) has been proposed for gluten determination by a commercial enzyme-linked immunosorbent assay (ELISA). Fourteen NADESs were prepared by combining two natural primary metabolites and water. Studies on NADES viscosity and gluten solubilisation in NADESs and ethanol-water solutions (for comparison purposes) were carried out. Different strategies for speeding-up gluten solubilisation in NADESs were evaluated: dilution, temperature and sonication by a cup-horn sonoreactor. Diluted fructose-citric acid NADES and sonication were finally selected for gluten solubilisation. Solubilised proteins were characterized by electrophoresis and molecular fluorescence. The proposed procedure was also assessed in real samples, especially ultrasound time. Kit solvents (including reducing agents) were replaced by NADES, and hence, a reassessing of immunoassay system was necessary. Samples with and without gluten as well as recovery tests were used for this purpose. Recoveries were in the range of 79-106% and the repeatability, expressed as relative standard deviation, was better than 15%.
2692 related Products with: Natural deep eutectic solvents in combination with ultrasonic energy as a green approach for solubilisation of proteins: application to gluten determination by immunoassay.Cell Meter™ Fluorimetri Amplite™ Intracellular Cell Meter™ Fluorimetri QuantiChrom™ Formaldehy EnzyChrom™ Kinase Assay Mouse Anti-Insulin-Like G MarkerGene™ Total Prote MOUSE ANTI BOVINE ROTAVIR Amplite™ Fluorimetric G Amplite™ Fluorimetric G Amplite™ Fluorimetric F EMAP-II Inhibitor Z-ASTD-
#27671713 2016/09/27 Save this To Up
Investigation on the anaerobic propionate degradation by Escherichia coli K12.Propionate is an abundant carboxylic acid in nature. Microorganisms metabolize propionate aerobically via the 2-methylcitrate pathway. This pathway depends on a series of three reactions in the citric acid cycle that leads to the conversion of succinate to oxaloacetate. Interestingly, the γ-proteobacterium Escherichia coli can use propionate as a carbon and electron source under oxic but not under anoxic conditions. RT-PCR and transcriptomic analysis revealed a posttranscriptional regulation of the prpBCDE-gene cluster encoding the necessary enzymes for propionate metabolism. The polycistronic mRNA seems to be hydrolyzed in the 3'-5' direction under anoxic conditions. This regulatory strategy is highly constructive because the last gene of the operon encodes the first enzyme of the propionate metabolism. Further analysis revealed that RNase R is involved in the hydrolysis of the prp transcripts. Consequently, an rnr-deletion strain could metabolize propionate under anoxic conditions. To the best of our knowledge, this is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli.
2742 related Products with: Investigation on the anaerobic propionate degradation by Escherichia coli K12.ESCHERICHIA COLI clinical ESCHERICHIA COLI 0111 NM Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Mouse Anti-Escherichia co Goat Anti-Escherichia col
#27578410 2016/09/13 Save this To Up
Size distribution analysis of influenza virus particles using size exclusion chromatography.Size exclusion chromatography is a standard method in quality control of biopharmaceutical proteins. In contrast, vaccine analysis is often based on activity assays. The hemagglutination assay is a widely accepted influenza quantification method, providing no insight in the size distribution of virus particles. Capabilities of size exclusion chromatography to complement the hemagglutination assay are investigated. The presented method is comparatively robust regarding different buffer systems, ionic strength and additive concentrations. Addition of 200mM arginine or sodium chloride is necessary to obtain complete virus particle recovery. 0.5 and 1.0M arginine increase the hydrodynamic radius of the whole virus particles by 5nm. Sodium citrate induces virus particle aggregation. Results are confirmed by dynamic light scattering. Retention of a H1N1v strain correlates with DNA contents between 5ng/mL and 670ng/mL. Quantitative elution of the virus preparations is verified on basis of hemagglutination activity. Elution of hemagglutination inducing compounds starts at a flow channel diameter of 7000nm. The universal applicability is demonstrated with three different influenza virus samples, including an industrially produced, pandemic vaccine strain. Size distribution of the pandemic H1N1v 5258, H1N1 PR/8/34, and H3N2 Aichi/2/68 preparations spreads across inter- and intra-particle volume and extends to the secondary interaction dominated range. Thus, virus particle debris seems to induce hemagglutination. Fragments generated by 0.5% Triton™ X-100 treatment increase overall hemagglutination activity.
1436 related Products with: Size distribution analysis of influenza virus particles using size exclusion chromatography.IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr
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