Search results for: DNM-9602A Microplate reader
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#38460614 2024/03/07 To Up
Development of toluidine red particle agglutination-based turbidimetric immunoassay for anticardiolipin antibody detection in syphilis.
Serological tests of non-treponemal and treponemal types are the most frequently used for syphilis diagnosis. Nontreponemal tests are used to monitor disease activity. Toluidine red unheated serum test (TRUST), as one of nontreponemal tests, is generally applicable to hospitals at different levels. However, accurate judgment of TRUST results is inseparable from an experienced and accurate operator. To reduce current shortcomings of manual TRUST method, we attempted to convert the manual TRUST test into automatic TRUST test, that is, to determine the degree of aggregation of toluidine red particles by detecting the absorbance value of serum after reaction with toluidine red particles.Xi Chen, Chengyu Yuan, Biwen Wang, Mengni Guan, Xianyu Zheng
2975 related Products with: Development of toluidine red particle agglutination-based turbidimetric immunoassay for anticardiolipin antibody detection in syphilis.
50ug100ug Lyophilized1 Set1 Set1 Set100ug Lyophilized 100ul1 Set1 SetRelated Pathways
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#38434906 2024/02/13 To Up
Fluorescently Modified NDM-1: A Versatile Drug Sensor for Rapid β-Lactam Antibiotic and Inhibitor Screening.
We successfully developed a fluorescent drug sensor from clinically relevant New Delhi metallo-β-lactamase-1 (NDM-1). The F70 residue was chosen to be replaced with a cysteine for conjugation with thiol-reactive fluorescein-5-maleimide to form fluorescent F70Cf, where "f" refers to fluorescein-5-maleimide. Our proteolytic studies of unlabeled F70C and labeled F70Cf monitored by electrospray ionization-mass spectrometry (ESI-MS) revealed that fluorescein-5-maleimide was specifically linked to C70 in 1:1 mole ratio (F70C:fluorophore). Our drug sensor (F70Cf) can detect the β-lactam antibiotics cefotaxime and cephalothin by giving stronger fluorescence in the initial binding phase and then declining fluorescence signals as a result of the hydrolysis of the antibiotics into acid products. F70Cf can also detect non-β-lactam inhibitors (e.g., l-captopril, d-captopril, dl-thiorphan, and thanatin). In all cases, F70Cf exhibits stronger fluorescence due to inhibitor binding and subsequently sustained fluorescence signals in a later stage. Native ESI-MS results show that F70Cf can bind to all four inhibitors. Moreover, our drug sensor is compatible with a high-throughput microplate reader and has the capability to perform drug screening.Sai-Fung Chung, Suet-Ying Tam, Wai-Ting Wong, Pui-Kin So, Wing-Lam Cheong, Chun-Wing Mak, Leo Man-Yuen Lee, Pak-Ho Chan, Kwok-Yin Wong, Yun-Chung Leung
1513 related Products with: Fluorescently Modified NDM-1: A Versatile Drug Sensor for Rapid β-Lactam Antibiotic and Inhibitor Screening.
400Tests100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assaysRelated Pathways
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#38405497 2024/02/06 To Up
Utilizing a Paper-Based Platform for Oilfield Applications: Time-Resolved Fluorescence Imaging and Detection of Interwell Chemical Tracers.
Chemical tracers are indispensable tools for enhancing reservoir characterization and optimizing production processes in the oil and gas industry. Particularly, interwell water tracers provide key data for efficient water flood management and the improvement of production rates. However, the analysis of these water tracers within reservoir fluids is challenging, requiring laborious separation and extraction steps that often rely on complex instruments and skilled operators. Real-time analysis is especially problematic in remote areas with limited access to well-equipped laboratories. To address these challenges, we introduce a paper-based platform for the time-resolved fluorescence detection of dipicolinic acid (DPA) tracers complexed with terbium ion (Tb). Our innovation is driven by the need to simplify tracer analysis, make it portable, and enhance accessibility for oilfield applications. By leveraging the unique properties of cyclen-based macrocyclic ligands, we have achieved the stable and sensitive immobilization of Tb on quartz microfilter paper, eliminating the need for extensive laboratory-based procedures. We achieve the stable and sensitive immobilization of Tb on quartz microfilter paper by leveraging the unique properties of cyclen-based macrocyclic ligands. This innovation enables the formation of highly fluorescent, oil-blind, and optically detectable DPA-Tb complexes at the paper surface. We visualize and capture these fluorescence signals using an intensified charge-coupled device camera via time gating, effectively suppressing undesirable fluorescence originating from crude oil. The quantification of DPA concentrations is achievable down to 158 ppb (9.45 × 10 M), as confirmed through time-resolved fluorescence microplate reader measurements. We also demonstrate the practicality of our technology by detecting DPA tracers in the presence of crude oil contamination, a common challenge encountered in oil production wells.Bora Yoon, Kiera Y Tai, Gawain M Thomas, Hooisweng Ow, Sehoon Chang
2043 related Products with: Utilizing a Paper-Based Platform for Oilfield Applications: Time-Resolved Fluorescence Imaging and Detection of Interwell Chemical Tracers.
1 ml1 LITRE25 µg0.2 mg100 ml100 TESTS1 kit0.1 mg25 µg1 kit0.1 mgRelated Pathways
#38389969 2024/01/15 To Up
and King fruit peel extract: Secondary metabolite composition, antioxidant, and elastase inhibitory activity evaluation.
and King are native plants from Indonesia and have tremendous potential as a source of raw medicines based on local wisdom. However, scientific data for strengthening pharmaceuticals are still limited. Therefore, it is necessary to conduct a study to strengthen and develop the potential of both plants using the approach of traditional medicine. This study aimed to explore the secondary metabolite composition and biological activity (antioxidant and antielastase) of both plants. Both samples were extracted using 70% ethanol and microwave-assisted extraction with a microwave power of 120 watts for 15 min. The extract obtained was then screened for phytochemicals using specific reagents. The total phenolic content (TPC) was determined using spectrophotometry with a 96-well microplate reader method. The total flavonoid content (TFC) was determined using the colorimetric method, whereas metabolite profiling analysis was conducted using the UPLC-QToF-MS/MS system. Meanwhile, biological activity was tested for antioxidant activity and antielastase as measured by a microplate reader 96-well spectrophotometry method at specific wavelengths. According to the results, and fruit peel extracts showed positive detection of particular secondary metabolites. TPC and TFC values were 13.98 ± 1.90 mg GAE/g and 10.33 ± 1.90 mg QE/g for and 11.98 ± 2.04 mgGAE/g and 1.96 ± 0.36 mgQE/g for . Metabolite profiling detected some compounds from , including ephedrannin B, hinokiflavone, mahuannin J, and candidate mass CHO, and , including 5-Hydroxy-7,8,2'- trimethoxyflavone, lucialdehyde B, candidate mass CHNO, candidate mass CHO, and candidate mass CHO. Meanwhile, the biological activities (antioxidant and antielastase) were 137.721 μg/mL and 108.893 μg/mL for and 481.948 μg/mL and 250.611 μg/mL for , respectively. Both plants showed different profiles of secondary metabolites and biological activities (antioxidant and antielastase) according to their respective characteristics.Neneng Siti Silfi Ambarwati, Nurnisya Tiara Sukma, Yesi Desmiaty, Annisa Auliya, Setia Budi, M Arifuddin, Islamudin Ahmad
2061 related Products with: and King fruit peel extract: Secondary metabolite composition, antioxidant, and elastase inhibitory activity evaluation.
96T50 mg25 mg100ug10 mg1000 tests50 ug 100ug500 mg25 mg10 mg 5 GRelated Pathways
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