Search results for: Detection Buffer C&D Antibody Array
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#20864329 // To Up
Surface plasmon resonance biosensor for parallelized detection of protein biomarkers in diluted blood plasma.
Surface plasmon resonance (SPR) biosensor for high-throughput screening of protein biomarkers in diluted blood plasma is reported. The biosensor combines a high-resolution SPR imaging sensor and a high-density protein array with low-fouling background. The SPR imaging sensor utilizes polarization contrast and advanced referencing and provides a total of 120 sensing areas (each 200 μm×150 μm). Antibodies are immobilized on the sensing areas via hybridization of antibody-oligonucleotide conjugates to thiolated complementary oligonucleotides microspotted on the sensor surface (DNA-directed immobilization). A low-fouling background is achieved by covalent immobilization of bovine serum albumin to carboxyl-terminated thiols filling the areas among the thiolated oligonucleotides and outside the sensing areas. The biosensor was evaluated for detection of protein biomarkers relevant to cancer diagnostics--human chorionic gonadotropin (hCG) and activated leukocyte cell adhesion molecule (ALCAM) both in buffer and in 10% blood plasma. Limits of detection as low as 45 ng/mL (ALCAM) and 100 ng/mL (hCG) were achieved in blood plasma samples.Marek Piliarik, Markéta Bocková, Jiří Homola
1610 related Products with: Surface plasmon resonance biosensor for parallelized detection of protein biomarkers in diluted blood plasma.
0.05 mg1mg96T1 Set1 Set1 Set1mg100ug Lyophilized1 Set1 Set1 Set100 μgRelated Pathways
#18958451 2008/10/29 To Up
Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging.
In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.Jon Ladd, Allen D Taylor, Marek Piliarik, Jiri Homola, Shaoyi Jiang
1046 related Products with: Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging.
100tests1 Bottle/Unit30 Samples1 mgEach1 x 5mlRelated Pathways
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