Search results for: DirectPCR Lysis Reagent (cell)
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Cell lysis via acoustically oscillating sharp edges.In this article, we demonstrate an acoustofluidic device for cell lysis using the acoustic streaming effects induced by acoustically oscillating sharp-edged structures. The acoustic streaming locally generates high shear forces that can mechanically rupture cell membranes. With the acoustic-streaming-derived shear forces, our acoustofluidic device can perform cell lysis in a continuous, reagent-free manner, with a lysis efficiency of more than 90% over a range of sample flow rates. We demonstrate that our acoustofluidic lysis device works well on both adherent and non-adherent cells. We also validate it using clinically relevant samples such as red blood cells infected with malarial parasites. Additionally, the unique capability of our acoustofluidic device was demonstrated by performing downstream protein analysis and gene profiling without additional washing steps post-lysis. Our device is simple to fabricate and operate while consuming a relatively low volume of samples. These advantages and other features including the reagent-free nature and controllable lysis efficiency make our platform valuable for many biological and biomedical applications, particularly for the development of point-of-care platforms.
DirectPCR Lysis Reagent ( 129 Mouse Embryonic Stem ATP Cell Viability Assay CellQuanti Blue™ Cell V ECOS™ 9-5 Competent Cel Cell Lysis Buffer 400 ml anti Transferrin receptor Cell Meter™ Cell Viabil Hygromycin B, EvoPure™, CellQuanti MTT™ Cell Vi Cell Lysis Buffer Rat Mesenchymal Cells
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Immunoglobulin for Treating Bacterial Infections: One More Mechanism of Action.The mechanisms underlying the effects of immunoglobulins on bacterial infections are thought to involve bacterial cell lysis via complement activation, phagocytosis via bacterial opsonization, toxin neutralization, and antibody-dependent cell-mediated cytotoxicity. Nevertheless, recent advances in the study of the pathogenicity of Gram-negative bacteria have raised the possibility of an association between immunoglobulin and bacterial toxin secretion. Over time, new toxin secretion systems like the type III secretion system have been discovered in many pathogenic Gram-negative bacteria. With this system, the bacterial toxins are directly injected into the cytoplasm of the target cell through a special secretory apparatus without any exposure to the extracellular environment, and therefore with no opportunity for antibodies to neutralize the toxin. However, antibodies against the V-antigen, which is located on the needle-shaped tip of the bacterial secretion apparatus, can inhibit toxin translocation, thus raising the hope that the toxin may be susceptible to antibody targeting. Because multi-drug resistant bacteria are now prevalent, inhibiting this secretion mechanism is an attractive alternative or adjunctive therapy against lethal bacterial infections. Thus, it is not unreasonable to define the blocking effect of anti-V-antigen antibodies as the fifth mechanism for immunoglobulin action against bacterial infections.
2451 related Products with: Immunoglobulin for Treating Bacterial Infections: One More Mechanism of Action.MOUSE ANTI BORRELIA BURGD Regorafenib Mechanisms: V Protein A (Liquid form) Formamide Molecular biolo Forskolin NVP-LDE-225 Mechanisms: H Mouse PAI-1 (wild type la RABBIT ANTI GSK3 BETA (pS MLN-4924 Mechanisms: NAE Rabbit PAI-1 (wild type l NATIVE HUMAN PROLACTIN, P Live Bacterial Gram Stain
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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation.Dynamic protein-protein interactions control cellular behavior, from motility to DNA replication to signal transduction. However, monitoring dynamic interactions among multiple proteins in a protein interaction network is technically difficult. Here, we present a protocol for Quantitative Multiplex Immunoprecipitation (QMI), which allows quantitative assessment of fold changes in protein interactions based on relative fluorescence measurements of Proteins in Shared Complexes detected by Exposed Surface epitopes (PiSCES). In QMI, protein complexes from cell lysates are immunoprecipitated onto microspheres, and then probed with a labeled antibody for a different protein in order to quantify the abundance of PiSCES. Immunoprecipitation antibodies are conjugated to different MagBead spectral regions, which allows a flow cytometer to differentiate multiple parallel immunoprecipitations and simultaneously quantify the amount of probe antibody associated with each. QMI does not require genetic tagging and can be performed using minimal biomaterial compared to other immunoprecipitation methods. QMI can be adapted for any defined group of interacting proteins, and has thus far been used to characterize signaling networks in T cells and neuronal glutamate synapses. Results have led to new hypothesis generation with potential diagnostic and therapeutic applications. This protocol includes instructions to perform QMI, from the initial antibody panel selection through to running assays and analyzing data. The initial assembly of a QMI assay involves screening antibodies to generate a panel, and empirically determining an appropriate lysis buffer. The subsequent reagent preparation includes covalently coupling immunoprecipitation antibodies to MagBeads, and biotinylating probe antibodies so they can be labeled by a streptavidin-conjugated fluorophore. To run the assay, lysate is mixed with MagBeads overnight, and then beads are divided and incubated with different probe antibodies, and then a fluorophore label, and read by flow cytometry. Two statistical tests are performed to identify PiSCES that differ significantly between experimental conditions, and results are visualized using heatmaps or node-edge diagrams.
1741 related Products with: Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation.TNFRSF1A & IKBKB Protein MMP1 & F2R Protein Protei CACYBP & CTNNB1 Protein P IKBKB & RELA Protein Prot TRAF2 & TRAF1 Protein Pro STAT1 & PDGFRB Protein Pr IL1B & A2M Protein Protei STAT1 & PTK2 Protein Prot ACTN1 & CAMK2A Protein Pr PIK3R1 & CSF1R Protein Pr CRKL & PIK3R1 Protein Pro CUL1 & FBXW11 Protein Pro
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Monitoring of dynamic ATP level changes by oligomycin-modulated ATP synthase inhibition in SW480 cancer cells using fluorescent "On-Off" switching DNA aptamer.Adenosine triphosphate (ATP) is the main energy source in cells and an important biomolecule participating in cellular reactions in living organisms. Since the ATP level changes dynamically reflecting the development of a debilitating disease or carcinogenesis, we have focused in this work on monitoring of the oligomycin (OMC)-modulated ATP synthase inhibition using a fluorescent-switching DNA aptamer designed for the detection of ATP (Apt(ATP)), as the model for studies of dynamic ATP level variation. The behavior of the ATP aptamer has been characterized using fluorescence spectroscopy. The Intramolecular fluorescence resonance energy transfer (iFRET) operates in the proposed aptamer from the FAM dye moiety to guanines of the aptamer G-quadruplex when the target ATP is present and binds to the aptamer changing its conformation. The iFRET process enables the detection of ATP down to the limit of detection, LOD = 17 μM, without resorting to any extra chemi-amplification schemes. The selectivity coefficients for relevant interferent triphosphates (UTP, GTP, and CTP) are low for the same concentration as that of ATP. We have demonstrated an efficient transfection of intact cells and OMC-treated SW480 colon cancer cells with Apt(ATP), using microscopic imaging, iFRET measurements, and cell viability testing with MTT method. The applicability of the switching DNA aptamer for the analysis of real samples, obtained by lysis of SW480 cells, was also tested. The proposed Apt(ATP) may be considered as a viable candidate for utilization in measurements of dynamic ATP level modulation in cells in different stages of cancer development and testing of new drugs in pharmacological studies. Graphical abstract.
1324 related Products with: Monitoring of dynamic ATP level changes by oligomycin-modulated ATP synthase inhibition in SW480 cancer cells using fluorescent "On-Off" switching DNA aptamer.Goat Anti-Human ATP13A1, Recombinant E. coli HSP70 AtpB | beta subunit of AT Recombinant E. coli HSP70 ATPase | ATP synthase, wh Goat Anti- ATP7A, (intern AtpB | beta subunit of AT Recombinant E. coli HSP70 Thyroid cancer and normal Rabbit Anti-ATP7B Polyclo Lung cancer survey tissue Mid advanced stage uterin
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Intraoperative tumor lysis syndrome in a giant teratoma: a case report.Tumor lysis syndrome is an unusual metabolic emergency in solid tumors. Perioperative occurrence of this syndrome is extremely rare but may have fatal consequences if not detected and treated on time.
Multiple organ tumor tiss Brain tumor tissue array Breast tumor survey tissu Colon tumor survey tissue Breast tumor survey tissu Liver tumor tissue array, Breast tumor survey tissu Breast tumor survey tissu Multiple organ tumor with Bone marrow tumor and nor Multiple organ stromal tu Multiple organ tumor and
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Evaluation of automated erythrocyte methodology in new world camelids using the ADVIA 2120 hematology analyzer.Accurate erythrocyte measurements with ADVIA hematology analyzers require isovolumetric cell sphering in one reaction and hemolysis in another. However, camelid erythrocytes are resistant to sphering and osmotic lysis, and no published evaluation of ADVIA methods for camelids exists.
2298 related Products with: Evaluation of automated erythrocyte methodology in new world camelids using the ADVIA 2120 hematology analyzer.Blood Analyzer Animal Aut FDA Standard Frozen Tissu Eosin 5 isothiocyanate, R FDA Standard Frozen Tissu Blood Analyzer Auto Hemat Multiple organ tumor tiss XFA6000 Auto hematology a MultiGene Gradient therm FDA Standard Frozen Tissu ELISA TEK™ MBM Thermal XFA6030 Veterinary auto h FDA Standard Frozen Tissu
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Effect of aspartame on the placenta of adult albino rat. A histological and immunohistochemical study.Aspartame is an artificial sweetener usually consumed by hundreds of millions of persons all over the world. Its metabolites can be toxic to many organs and there are only a few studies on the use of aspartame during gestation. The present study was designed to fully evaluate the effect of aspartame on the histological structure of the placenta in the adult albino rat. Twenty pregnant female rats were equally divided into group I that served as control, and group II that received aspartame at a dose 14 mg/kg by gavage on the 9th, 10th and 11th day of pregnancy. Placental specimens were processed for histological and immunohistochemical staining against vascular endothelial growth factor (VEGF). Aspartame induced a significant decrease in the mean placental weight and the mean thickness of both labyrinth and basal zones. Damage in the placenta was detected in the form of rupture of the interhemal membrane, lysis of glycogen trophoblast cells, spongiotrophoblast cells with vacuolated cytoplasm and darkly stained nuclei. A significant increase in vascular endothelial growth factor expression in both labyrinth and basal zones was detected. Ultrastructural examination showed fetal capillaries with condensed nuclei of endothelial cells, cytotrophoblasts with condensed fragmented nuclei and vacuolated cytoplasm, and syncytiotrophoblasts with irregular condensed fragmented nuclei. It could be concluded that aspartame has deeply impacted the normal structure and presumably the function of the placenta, therefore, restrictions are to be imposed on the consumption of aspartame especially during pregnancy.
2891 related Products with: Effect of aspartame on the placenta of adult albino rat. A histological and immunohistochemical study.Non Swiss Albino Mouse Pl Normal rat multiple organ Anti-Androgen Receptor pr 5α-N-Acetyl-2'H-androst- Non Swiss Albino Mouse Pl Thermal Shaker with cooli Androgen Receptor , Mouse 3β-O-Acetyl-androsta-5,1 Non Swiss Albino Mouse Pl 5α-Androstan-3β-ol � Advanced Airway Intubatio Androstane 3a, 17b diol 5
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A novel herbometallic nanodrug has the potential for antibacterial and anticancer activity through oxidative damage.Preparation of a herbometallic nano-drug, Rasa Manikya nanoparticle (RMNP) and investigation of its antimicrobial, and anticancer activity. Physicochemical characterizations of RMNP were performed using different analytical methods. The antimicrobial and anticancer potential of RMNPs were assessed by an cellular assay. Bacterial cell wall lysis was observed by field emission scanning electron microscopy and mitochondrial metabolism alteration factor was measured standard method. Physicochemical analysis confirmed that RMNP was rich in mineral constituents. Synergistic effect of RMNPs enhanced lysis of bacterial peptidoglycan layers and impaired cellular redox balance, GSH/NADPH level followed by induction of cell apoptosis. The present study confirms that RMNP can be used as a dual therapeutic option for combating drug-resistant microbial strains and breast cancer.
2281 related Products with: A novel herbometallic nanodrug has the potential for antibacterial and anticancer activity through oxidative damage.MarkerGene™ LysoLive™ 5-Acetylamino-6-formylami Catalase Fluorescent Acti MOUSE ANTI APAAP COMPLEX, Rabbit anti PKC theta (Ab Androgen Receptor (Phosph Cathepsin S Activity Assa Lymphoma array, together Tissue array of ovarian g Androgen Receptor (Phosph Rapid Microplate Assay K HDAC Colorimetric Activit
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