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Search results for: Dog IgA ELISA Kit

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#31410878   2019/08/13 To Up

Validation and method comparison of the use of densitometry to quantify monoclonal proteins in canine sera.

Densitometric quantitation using serum protein electrophoresis (SPE) is used to monitor monoclonal proteins (M-proteins) in human patients but has not been validated in the dog. Serum globulin concentrations, species-specific radial immunodiffusion (RID), and ELISAs are currently used in veterinary medicine.
Adam Dugger Harris, Emily Rout, Anne Avery, Denise Bolte, Erica Belling-Kelly, A Russell Moore

1954 related Products with: Validation and method comparison of the use of densitometry to quantify monoclonal proteins in canine sera.

50101 mg1mg1 mg

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#28669382   2017/05/30 To Up

New wide dynamic range assays for quantification of anti-Leishmania IgG2 and IgA antibodies in canine serum.

The aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-Leishmania IgG2 and IgA antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-Leishmania antibodies in Leishmania-seronegative and seropositive dogs, determined by a commercial ELISA assay, and between different clinical stages according to LeishVet guidelines. The analytical validation showed that the assays had a good precision with intra- and inter-assay coefficients of variation lower than 10%. In addition, the assays allowed the quantification of very low concentration of antibodies as well as demonstrated a high level of accuracy, as determined by linearity under dilution (R=0.99) and recovery tests (>85%). Moreover, no cross-reactions with Ehrlichia canis, Canine Parvovirus Type 2, Anaplasma phagocytophilum, Babesia canis, Dirofilaria immitis and pyometra were found. The assays were able to detect higher values of anti-Leishmania IgG2 and IgA antibodies in seropositive dogs compared with seronegative dogs (p<0.0001), although an overlap between groups existed in the case of IgA. In addition, significantly higher values for both antibodies were detected in LeishVet groups II (p<0.05) and III (p<0.01) when compared with LeishVet group I. From our study, it could be concluded that the immunofluorometric assays developed would be suitable for determination of anti-Leishmania IgG2 and IgA antibodies in serum samples with an adequate precision, analytical sensitivity and accuracy. In addition, these assays showed a wider difference in the concentration of both IgG2 and IgA antibodies between seronegative and seropositive dogs and between different clinical stages of CanL than a current commercial ELISA kit. Further studies would be recommended to evaluate the diagnostic sensitivity and specificity of these new assays as well as their application in monitoring CanL.
Ana Cantos-Barreda, Damián Escribano, Luis J Bernal, Luis Pardo-Marín, José J Cerón, Silvia Martínez-Subiela

2107 related Products with: New wide dynamic range assays for quantification of anti-Leishmania IgG2 and IgA antibodies in canine serum.

0.25 mg250 ug100 μg100 μg100 μg100 0.1 ml1 mg100 μg100 μg100 μg100 μg

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#16183897   // To Up

Proteinuria and immunoglobulinuria in neonatal dogs.

Samples of urine and serum from 45 newborn rottweiler puppies from six litters, and milk from their mothers, were taken 24, 48 and 72 hours and seven and 14 days after birth. Urine total protein and creatinine concentrations were determined and the ratios calculated. The immunoglobulin (Ig) concentrations of IgG, IgM and IgA in urine, serum and milk were determined with a commercially available elisa kit. The concentration of total protein in urine decreased from 1.64 to 0.29 mg/ml, and it and the ratio of total protein to creatinine in the urine of the neonatal puppies exceeded the normal values for adult dogs, but all the puppies developed normally. The average concentration of IgG in urine decreased from 0.0035 to 0.0003 mg/ml, that of IgA from 0.0035 to 0.0002 mg/ml and that of IgM from 0.0006 mg/ml to undetectable levels after two weeks. After two weeks, 47 per cent of the puppies had measurable levels of IgA and 70.6 per cent had measurable levels of IgG, but none of them had measurable levels of IgM.
S Schäfer-Somi, S Bär-Schadler, J E Aurich

1927 related Products with: Proteinuria and immunoglobulinuria in neonatal dogs.

100 μg1 Set100 μg1 Set100ug1 Set 25 G1 Set12

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