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#29053577   2017/10/20 Save this To Up

Electrographic Changes Accompanying Recurrent Seizures under Ketogenic Diet Treatment.

The ketogenic diet (KD) is increasingly used to treat epilepsy refractory to antiepileptic drugs and other neurological disorders. In animal models, the KD was found to increase the threshold to seizures induced by different convulsive stimulations. However, in models in which suprathreshold stimuli were used, a paradoxical seizure worsening was consistently observed in KD-fed animals. To better define this phenomenon, we characterized the electrographic response to seizures induced in mice which were treated with the KD, and then corneally stimulated at 6-Hz in four different sessions. We also evaluated the electroencephalogram (EEG) in three patients in which the KD was associated with a paradoxical worsening of epileptic seizures. Although seizures were initially less severe, a remarkable prolongation of the electrographic response was observed in mice receiving the KD from the second session of 6-Hz corneal stimulation and onwards. The EEG was also markedly altered in the presence of progressive seizure aggravation observed in children treated with the KD, specifically one affected by Lennox-Gastaut syndrome and two by type I lissencephaly,. These results suggest that when seizures are induced or recur because of resistance to therapeutic interventions, the KD may change the EEG by potentiating the electrographic epileptic activity.

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RH 414 [N (3 Triethylammo MM 4 64 [N (3 Triethylamm MM 2 10 [N (3 Triethylamm DiOC2(3) iodide [3,3 Diet DiSC2(3) [3,3 Diethylthia DiSC2(7) [3,3-Diethylthia DEAC [7 Diethylaminocouma DEAC,SE [7 Diethylaminoco Acetonedicarboxylic Acid (2-Acetamido-2,2-dicarbox 2-(Acetylamino)-2-[2-(4-b Acetyl-D,L-diethylalanine

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#29052263   2017/10/20 Save this To Up

ANG II-CCR2/5 axis dependent monocyte/macrophage recruitment contributed to experimental autoimmune myocarditis progression.

Angiotensin II (ANG II) plays critical roles in modulation of circulatory homeostasis and activation of innate and adaptive immunity. Furthermore, ANG II has also been implicated in several mouse models of autoimmune disease. However, it remains incompletely clear how ANG II regulates macrophage and involves in experimental autoimmune myocarditis (EAM) development. Therefore, the present work was to address the above question and to explore the possible mechanism. The BALB/c mice were used to induce EAM model. ANG II levels were detected by ELISA; HE staining was employed to analyze the pathological changes and macrophage infiltration. The chemotactic ability of ANG II was assessed by transwell system. Our results showed that ANG II was up-regulated in serum and heart tissues of EAM models and ANG II significantly drove monocyte/macrophage infiltration through C-C chemokine receptor 2/5 (CCR2/5) axis. CCR2/5 antagonists and ANG II receptor inhibitor all could abrogate monocyte/macrophage infiltration and ameliorate EAM development. Our results firstly addressed a novel function of ANG II: ANG II is a critical chemokine for monocyte/macrophage recruitment; furthermore, our results also indicated that ANG II might be a potential therapy target of inflammatory diseases.

1881 related Products with: ANG II-CCR2/5 axis dependent monocyte/macrophage recruitment contributed to experimental autoimmune myocarditis progression.

Angiotensin II [Lys0](Hum Tonsil disease spectrum ( Anti-Angiotensin II Recep Anti Angiotensin II Recep Anti-Angiotensin II Recep Anti-Angiotensin II Recep Anti Angiotensin II Recep Anti-Angiotensin II recep Anti-Angiotensin II recep Angiotensin II receptor ( Anti-Angiotensin II recep Topoisomerase II; Clone

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#29052023   2017/10/20 Save this To Up

The serum level and significance of lysyl oxidase-like 2 in patients with rheumatoid arthritis-associated interstitial lung disease.

Our previous experiments found that lysyl oxidase-like 2 (LOXL2) may be a useful preclinical serological marker for pulmonary fibrosis in the mouse model. The role of LOXL2 in rheumatoid arthritis-associated interstitial lung disease (RA-ILD) is still unclear. We investigated whether serum LOXL2 levels are associated with RA-ILD patients. The levels of serum LOXL2 were measured by enzyme-linked immunosorbent assay in 49 RA-ILD patients (21 patients with ILD disease duration < 3 months; 28 patients with ILD disease duration > 3 months), 43 RA patients without ILD and 20 normal healthy controls. We assessed the correlations between the serum LOXL2 levels and clinical variables. Serum LOXL2 levels were significantly higher in RA patients than in normal healthy controls (326.79 ± 192.56 vs. 53.27 ± 35.86 pg/ml, P < 0.01). No significant difference was present between the RA-ILD group and RA without ILD group (298.87 ± 219.85 vs. 358.60 ± 152.16 pg/ml, P = 0.13). Notably, the serum LOXL2 levels were significantly higher in patients with ILD disease duration < 3 months than in those with ILD disease duration > 3 months (462.71 ± 208.97 vs. 175.99 ± 130.55 pg/ml, P < 0.01) or without ILD (462.71 ± 208.97 vs. 358.60 ± 152.16 pg/ml, P = 0.03). The serum LOXL2 levels in RA-ILD patients significantly correlated with DAS28 (rs = 0.31, P = 0.034), C-reactive protein (rs = 0.41, P = 0.004), rheumatoid factor (rs = 0.41, P = 0.003), forced vital capacity (rs = - 0.39, P = 0.02), and diffusion capacity of the lung for carbon monoxide (rs = - 0.44, P = 0.009). LOXL2 may be involved in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and might be helpful in early diagnosis of RA-ILD.

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Lung disease spectrum tis Lung disease spectrum tis Lung cancer tissue array, Lung carcinoma and normal Colon disease spectrum (c Innovative Grade™ Canin Renal disease spectrum ti Non small cell lung carci Lung small cell carcinoma Lung disease spectrum tis Multiple lung carcinoma ( Lung cancer survey tissue

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#29050115   2017/10/19 Save this To Up

[Effects of ovalbumin exposure during pregnancy of mice on the ovalbumin re-exposure in adult progeny].

Objective: To observe the immunoreaction of offspring mice by ovalbumin (OVA) re-exposure after their mothers exposed to OVA during pregnancy. Method: A prospective controlled study was conducted to observe mice after repeated OVA exposures at 6-8 weeks.Their mothers were exposed to OVA during different stages of pregnancy.The symptoms were recorded and scored.The levels of OVA-specific IgE in serum, interferon-γ(IFN-γ) and interleukin-4(IL-4) in supernatant of spleen primary lymphocytes in vitro were measured by ELISA.The results were analyzed by single factor analysis of variance or rank sum test. Result: All the mice in each group had acute diarrhea.The diarrhea happened earliest (2 days) and most severe in the late pregnancy group (early pregnancy group 7.0±1.0; middle pregnancy group: 7.1±1.1; late pregnancy group: 9.9±2.2, P<0.01). The levels of absorbance of OVA-specific IgE in the pregnancy groups were higher than those of the control group.The absorbance of OVA-specific IgE in late pregnancy group was the highest (control: 0.27±0.06; early pregnancy group: 0.51±0.13; middle pregnancy group: 0.50±0.09; late pregnancy group: 0.63±0.13, P<0.01). There was no significant change in IFN-γ expression in cultured supernatant of spleen lymphocytes in each group (control: (133±7) pg/ml; early pregnancy group: (133±4) pg/ml; middle pregnancy group: (134±6) pg/ml; late pregnancy group: (132±4) pg/ml, all P value >0.05). The expression of IL-4 in the experimental groups was higher than that in the control group, especially in late pregnancy group(control: (25.3±2.4) pg/ml; early pregnancy group: (32.4±4.4) pg/ml; middle pregnancy group: (35.0±5.4) pg/ml; late pregnancy group: (47.1±5.8) pg/ml; P value all<0.01). Conclusion: The allergic reaction of the OVA re-exposure progeny whose mothers were exposed to OVA in the late pregnancy period was most severe, suggesting that late pregnancy period might be the high risk stage of intrauterine sensitization, or"window period".

2775 related Products with: [Effects of ovalbumin exposure during pregnancy of mice on the ovalbumin re-exposure in adult progeny].

Thermal Shaker with cooli Rabbit Anti-FGF3 Oncogene Mouse Anti-Ovalbumin Anti Rabbit Anti-Chicken Ovalb Mouse anti-ovalbumin spec Mouse ovalbumin specific Goat anti-ovalbumin speci Goat ovalbumin specific I FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu

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#29050114   2017/10/19 Save this To Up

[Effect of interferon-γ on airway inflammation following respiratory syncytial virus reinfection in mice].

Objective: To identify the role of interferon (IFN)-γ during respiratory syncytial virus (RSV) re-infection in mice. Method: Female wild type C57BL/6 mice and IFN-γ knockout mice (IFN-γ(-/-) mice) at the age of 6 to 8 weeks were randomly divided into two groups: control group and RSV group, according to random number table.Each group was further divided into primary infection group and re-infection group.There were 8 groups.Mice were sacrificed on days 5, 7, 14 to collect samples.There were 5-8 mice in each group at each time point.And experiment was repeated twice. Leukocytes in bronchoalveolar lavage fluid (BALF) were counted, left lung tissues were stained with HE and histopathological scoring (HPS) was performed.The concentrations of IFN-γ, IL-5, IL-13 were determined with ELISA.T test or single factor analysis of variance was used to compare between groups. Result: (1) Mice infected or reinfected with RSV showed pale hair, weight loss, decreased activity and anorexia.(2) IFN-γ levels significantly increased on days 5 and 7 following RSV primary infection and reinfection as compared to control groups in wild type mice ((192±44) vs.(36±8) and (531±161) vs.(23±4) pg/ml on day 5, (100±23) vs.(36±8) and (862±186) vs.(23±4) pg/ml on day 7, t=2.654, 2.513, 2.654, 3.968, all P<0.05). (3) Compared to the RSV-reinfected IFN-γ(-/-) mice, RSV-reinfected wild type mice had less body weight loss ((13.6±2.6)% vs.(22.7±2.9)% on day 5, (18.0±3.1)% vs.(26.5±1.8)% on day 7, t=2.314, 2.308, both P<0.05), lower lung tissue histopathological score ((1.50±0.09) vs.(2.07±0.11) on day 5, (1.53±0.11) vs.(2.08±0.09) on day 7, (1.10±0.06) vs.(1.59±0.08) on day 14, t=3.916, 3.890, 4.837, all P<0.01), less BALF inflammatory cells count ((11.6±2.0) vs.(44.2±10.6)×10(5)/ml on day 5, (18.2±3.9) vs.(38.3±2.2)×10(5)/ml on day 7, t=2.818, 4.786, both P<0.05), and lower levels of IL-5 and IL-13 ((24±3) vs.(148±23), (23±4) vs.(169±26) pg/ml on day 5, (30±8) vs.(233±44), (20±5) vs.(182±19) pg/ml on day 7, (91±6) vs.(129±19), (62±8) vs.(132±5) pg/ml on day 14, t=5.252, 5.445, 4.517, 7.326, 3.816, 7.577, all P<0.01). Conclusion: IFN-γ can alleviate airway inflammation following RSV reinfection in mice.

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Anti-BRSV(Bovine Respirat Interferon γ Human Epstein-Barr Virus Human Interferon-gamma IF Interferon-a Receptor Typ Mouse Interferon gamma IF Mouse Epstein-Barr Virus Interferon alpha-8 antibo Interferon alpha-6 antibo Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe

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#29050075   2017/10/19 Save this To Up

[Specific cytotoxicity of a novel HER2-based chimeric antigen receptor modified T lymphocytes against HER2-positive tumor cells].

Objective: To construct the third generation chimeric antigen receptor based on a novel humanized anti-HER2 H1-2 scFv, and to investigate the specific cytotoxicity of H1-2 CAR modified T lymphocytes(CAR-T) against HER2(+) tumor cells. Method: The expression cassette of the third generation CAR gene and anti-HER2 H1-2 scFv were constructed and cloned into lentivirus transfer plasmid, and then the third generation H1-2 CAR was transduced into human T lymphocytes using lentivirus.Enzyme linked immunosorbent assay was used to detect the expression of cytokines IL2, and LDH release assay was used to detect the cytotoxic effect of the H1-2 CAR-T.Finally, NOD/SCID mice and HER2(+) breast cancer cell line SKBR3 were used to detect the anti-tumor effect of H1-2 CAR-T in vivo. Results: The third generation H1-2 CAR was successfully constructed.H1-2 CAR-T secreted high dose of IL2 after confrontation with HER2(+) breast cancer cells.In vitro, the cytolytic rate of H1-2 CAR-T on high expression HER2(+) tumor cells was significantly higher than that in low expression HER2 or non-expression HER2 tumor cells. At the efficacy to target ratio of 20, the cytolytic rate of H1-2 CAR-T against breast cancer cell SK-BR-3 could reach (90.1±2.8)%, while the cytolytic rate of H1-2 CAR-T against HER2(-) breast cancer cell MDA-MB-231 was only (13.5±4.7)%. In the mouse xenograft tumor model, H1-2 CAR-T cells inhibited breast cancer growth in vivo.At the end of the experiments, the average tumor weight in the H1-2 CAR-T cell treatment group was (0.7±0.1) g, the non-transfected T cell therapeutic group was (1.2±0.2) g, and the PBS group was (1.2±0.2) g. There was significant difference between the H1-2 CAR-T therapeutic group and the non-transfected T cell therapeutic group (P<0.05). However, there was no significant difference between the non-transfected T cell therapeutic group and the PBS treatment group (P>0.05). Conclusion: The HER2-sepcific H1-2 CAR-T cells specifically kill HER2 positive cells, and further studies on CAR-T cells for the treatment of HER2(+) cancers are useful.

2562 related Products with: [Specific cytotoxicity of a novel HER2-based chimeric antigen receptor modified T lymphocytes against HER2-positive tumor cells].

TNFRSF1B - Goat polyclona anti Transferrin receptor HER2 (Phospho Tyr877) Ant HER2(Phospho Tyr1221 Tyr1 HER2 (Phospho Tyr1248) An MOUSE ANTI BORRELIA BURGD RANK Ligand Soluble, Huma anti CD38 Hematopoietic p Breast cancer tissue arra Breast cancer tissue arra Dog Receptor-binding canc FDA normal and tumor orga

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#29047038   2017/10/19 Save this To Up

The Regulatory Roles of Toll-Like Receptor 4 in Secretions of Type 1/Type 2 Relative Cytokines by Splenocytes and Dendritic Cells Exposed to Clonorchis sinensis Excretory/Secretory Products.

The roles of TLR4 in mediation of innate immune response and in regulation of adaptive immune responses triggered by Clonorchis sinensis remain unknown. In the present study, splenocytes derived from C3H/HeN (TLR4 (wild) ) and C3H/Hej mice (TLR4 (mut) ) that were infected with 45 metacercariae of C. sinensis were harvested, then stimulated by C. sinensis excretory/secretory products (ESP) or medium (control) for 48 h, respectively. Meanwhile, bone marrow-derived dendritic cells (BMDCs) from normal C3H/HeN and C3H/Hej mice were prepared and stimulated with medium, ESP, LPS, or ESP+LPS for 24 h, respectively. The supernatants were collected, and the concentrations of type 1 and type 2 relative cytokines were determined by ELISA. The maturation of BMDCs indicated by surface markers of CD80, CD86, and MHC II was evaluated by flow cytometry. The results showed that the levels of IFN-γ, IL-6, TNF-α, and IL-10 in the splenocytes from C. sinensis-infected TLR4 (mut) mice were significantly lower than those from TLR4 (wild) mice when they were further exposed to ESP. For BMDCs, the productions of the cytokines IL-12p70 and IL-10, but not IL-4, in the BMDCs from TLR4 mutation mice were predominantly decreased compared with those from TLR4 (wild) mice when the BMDCs were co-stimulated by ESP combined with LPS. Flow cytometry analysis showed that ESP could significantly decrease the high levels of CD80, CD86, and MHC II which were elevated by LPS. In conclusion, these data suggest that TLR4 may play a regulatory role in type 1 immune responses during C. sinensis infection.

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Top 4 types of cancer (co Top 4 types of cancer (co Tissue microarray of top Interferon-a Receptor Typ Multiple human normal org High density (188 cases 2 High density (188 cases 2 High density (208 cores), Multiple cancer (12 type) Multiple cancer (12 type) Multiple types of cancer Multiple types of cancer

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#29045972   2017/10/18 Save this To Up

[Acetylsalicylic acid treatment enhanced immunomodulatory function of mesenchymal stem cells derived from gingiva].

To analyze the role of acetylsalicylic acid (ASA) in immunomodulation of mesenchymal stem cells derived from gingiva (GMSCs), and to explore the role of ASA in enhancing the immumomodulation of GMSCs and the capacity of GMSCs to treat immune disorders and the underlying mechanism.

1502 related Products with: [Acetylsalicylic acid treatment enhanced immunomodulatory function of mesenchymal stem cells derived from gingiva].

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#29045565   2017/10/18 Save this To Up

Cigarette sidestream smoke delays nucleotide excision repair - inhibited accumulation of repair proteins at DNA lesions.

Cigarette sidestream smoke (CSS) contains many carcinogens that induce DNA damage. DNA damage plays an important role in the initiation of cancer and several diseases, and repair is the major defense mechanism; however, the relationship between CSS and the repair of DNA damage remains unclear. We herein investigated whether CSS influences nucleotide excision repair (NER) in vivo and in vitro. HR-1 hairless mouse skin treated with CSS was exposed to UVB, as a result of which pyrimidine dimers (cyclobutane pyrimidine dimers (CPDs) and pyrimidine(6-4)pyrimidone photoproducts (6-4PPs)) were formed and repaired via the NER pathway. The immunohistochemical staining of CPDs revealed that their repair was delayed by the CSS treatment. This delay in NER and the underlying mechanisms were examined in the human skin cell lines, HaCaT and HSC-1. Dot-blot assays, ELISA, and local UV irradiation assays demonstrated that CSS delayed the repair of CPDs and 6-4PPs. The recruitment of the repair molecules, TFIIH, XPA, and XPG to pyrimidine dimers was markedly inhibited by CSS. Semicarbazide, which reacts with aldehydes, recovered the CSS-induced inhibition of NER, and formaldehyde exerted similar inhibitory effects to those of CSS. These results suggest that aldehydes in CSS interfere with the recruitment of NER molecules to damaged sites, leading to a delay in the repair of pyrimidine dimers.

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#29045486   2017/10/18 Save this To Up

Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.

Thiamine is an essential enzyme cofactor required for proper metabolic function and maintenance of metabolism and energy production in the brain. In developed countries, thiamine deficiency (TD) is most often manifested following chronic alcohol consumption leading to impaired mitochondrial function, oxidative stress, inflammation and excitotoxicity. These biochemical lesions result in apoptotic cell death in both neurons and astrocytes. Comparable histological injuries in patients with hypoxia/ischemia and TD have been described in the thalamus and mammillary bodies, suggesting a congruency between the cellular responses to these stresses. Consistent with hypoxia/ischemia, TD stabilizes and activates Hypoxia Inducible Factor-1α (HIF-1α) under physiological oxygen levels. However, the role of TD-induced HIF-1α in neurological injury is currently unknown. Using Western blot analysis and RT-PCR, we have demonstrated that TD induces HIF-1α expression and activity in primary mouse astrocytes. We observed a time-dependent increase in mRNA and protein expression of the pro-apoptotic and pro-inflammatory HIF-1α target genes MCP1, BNIP3, Nix and Noxa during TD. We also observed apoptotic cell death in TD as demonstrated by PI/Annexin V staining, TUNEL assay, and Cell Death ELISA. Pharmacological inhibition of HIF-1α activity using YC1 and thiamine repletion both reduced expression of pro-apoptotic HIF-1α target genes and apoptotic cell death in TD. These results demonstrate that induction of HIF-1α mediated transcriptional up-regulation of pro-apoptotic/inflammatory signaling contributes to astrocyte cell death during thiamine deficiency.

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Mouse Insulin-like Growth Mouse Factor X total anti Mouse Anti-Insulin-Like G Mouse Insulin-like Growth Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Hamster anti mouse Insuli Rat monoclonal anti mouse ELISA Mouse , Interleukin

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