Product name :InsertFinder PCR Quick Screening Kit

Catalog Number :K903-500

Quantity :500 samples

Price :303 Eur

Pay now with :

Supplier :Biovision

 

Kit Summary: 
• Application- Kit is designed to quickly screen large number of cloning inserts

Features & Benefits: 
• Procedure is quick and simple 
• Clones can be analyzed directly on an agarose gel

Kit components: 
• InsertFinder Lysis Buffer 
• 10X PCR Buffer 
• dNTP 
• Taq DNA Polymerase 
• PCR Enhancer

Description: 
Identification of clones that contain the desired DNA insert is an essential step in molecular cloning procedure. Traditional methods for identifying the correct DNA inserts require growing up individual colonies, isolating plasmid DNAs, and then digesting with restriction enzymes to analyze each individual clone. Such traditional procedures are very time consuming, laborious, and expensive. The new InsertFinderTM PCR Quick Screening Kit is designed to quickly screen clone candidates using PCR technology. The procedure allows screen of colonies directly from plate, without growing up the cultures. The proprietary Cell Lysis Buffer provided with the kit can efficiently lyse cells (bacteria or yeast) without interfering with PCR reactions. The PCR Enhancer included in the kit allows amplification of hairpin structure or GC-rich region efficiently. PCR products can directly be analyzed on an agarose gel without enzyme digestions. The kit provides a simple, convenient, and economical way to identify correct inserts. It is also suitable for screening large numbers of colonies.

Storage Conditions: 
-20°C

Shipping Conditions: 
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

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PLDS-PLAO Peptoid Ligand Affinity Optimization Drug Discovery Service Peptoid Ligand Screening Ray Biotech 0 PLDS-PLAO Peptoid Ligand Affinity Optimization Drug Discovery Service Peptoid Ligand Screening Ray Biotech 0€
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PLDS-PLD Peptoid Ligand Discovery Drug Discovery Service Peptoid Ligand Screening Ray Biotech 0 PLDS-PLD Peptoid Ligand Discovery Drug Discovery Service Peptoid Ligand Screening Ray Biotech 0€
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SIAB.CE Syph Ab (Syphilis screening) DiaPro 192 Tests n 1096 SIAB.CE Syph Ab (Syphilis screening) DiaPro 192 Tests n 1096€
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SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.368 SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.368€ Pub
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ST1003 Stomach cancer tissue array for antibody screening, including TNM, clinical stage and pathology grade, 50 cases 100 cores US Biomax 231 ST1003 Stomach cancer tissue array for antibody screening, including TNM, clinical stage and pathology grade, 50 cases 100 cores US Biomax 231€
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TORCHM.CE TORCH IgM screening (capture) DiaPro 96 Tests n 1614 TORCHM.CE TORCH IgM screening (capture) DiaPro 96 Tests n 1614€
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UNC241 Universal control tissue array, used for screening of more than 90% routine IHC markers, 12 cases 24 cores US Biomax 77 UNC241 Universal control tissue array, used for screening of more than 90% routine IHC markers, 12 cases 24 cores US Biomax 77€
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UT1001 Endometrial cancer tissue array for antibody screening, including TNM, clinical stage and pathology grade, 25 cases 100 cores US Biomax 266 UT1001 Endometrial cancer tissue array for antibody screening, including TNM, clinical stage and pathology grade, 25 cases 100 cores US Biomax 266€
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