Product name :EnzyChrom™ Kinase Assay Kit, Rapid mix-and-measure procedure for determination of kinase activity and high-throµghput screening for kinase inhibitors by fluorimetric (530nm 590nm) method

Catalog Number :EKIN-400

Quantity :400Tests

Price :250 Eur

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Supplier :Bioassays

 

DESCRIPTION
KINASES, also known as phosphotransferases, constitute a large family
of enzymes that transfer phosphate groups from the high-energy donor
molecule ATP, to their specific substrates. Kinases are known to regulate
the majority of cellular processes. The largest group of this family is the
protein kinases. So far, 518 different kinases have been identified in
humans and up to 30% of human proteins are modified by these kinases.
The enormous diversity and their key role in cellular signaling make them
ideal targets for drug developments.
BioAssay Systems’ EnzyChromTM Kinase Assay Kit provides a simple and
rapid method for assaying kinase activity and high-throughput screening
for kinase inhibitors. This homogeneous microplate-based assay involves
incubating the kinase with a single working reagent, in which ADP is
enzymatically converted to ATP and pyruvate, which is quantified using a
fluorimetric (530nm/590nm) assay method.
 
KEY FEATURES
Safe. Non-radioactive assay.
Sensitive and accurate. As low as 0.01 U/L kinase can be quantified.
Homogeneous and convenient. "Mix-incubate-measure" type assay.
The whole assay involves adding a single working reagent and incubation
for 10 min at room temperature.
Robust and amenable to HTS: Assay can tolerate up to 300 μM ATP
and 10% dimethylsulfoxide (DMSO). Z’ factors of > 0.6 are routinely
observed in 96/384-well plates. Can be readily automated on HTS liquid
handling systems for tens of thousands of assays per day.
 
APPLICATIONS
Kinase activity assays and HTS for kinase inhibitors.
ADP determination in cells and other biological samples.
 
KIT CONTENTS
Reagent A: 10 mL R eagent B: 10 mL
Assay Buffer: 25 mL S t andard: 100 μL 3 mM ADP
 
Storage conditions: store all reagents at -20°C. This product is shipped
on dry ice. Shelf life: 6 months after receipt.
 
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
datasheet of product :EnzyChrom™ Kinase Assay Kit, Rapid mix-and-measure procedure for determination of kinase activity and high-throµghput screening for kinase inhibitors by fluorimetric (530nm 590nm) method EnzyChrom™ Kinase Assay Kit, Rapid mix-and-measure procedure for determination of kinase activity and high-throµghput screening for kinase inhibitors by fluorimetric (530nm 590nm) method

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