Product name :EnzyFluo™ D-Lactate Assay Kit

Catalog Number :EFDLC-100

Quantity :100 tests

Price :285 Eur

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Supplier :Bioassays

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
Method: FL530/585 nm.
Samples: serum, plasma, cell culture media etc.
Species: all.
Procedure: 60 min.
Size: 100 tests.
Detection limit: 1 μM .

DESCRIPTION
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or
anaerobic conditions. Monitoring lactate levels is, therefore, a good
indicator of the balance between tissue oxygen demand and utilization and
is useful when studying cellular and animal physiology. D-Lactate is
produced in only minor quantities in animals and measuring for D-lactate in
animal samples is a means to determine the presence of bacterial infection.
Simple, direct and automation-ready procedures for measuring lactate
concentration are very desirable. BioAssay Systems' EnzyFluoTM lactate
assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate,
in which the formed NADH reduces a probe into a highly fluorescent
product. The fluorescence intensity of this product, measured at lex/em =
530/585 nm, is proportional to the lactate concentration in the sample.
APPLICATIONS
Direct Assays: D-lactate in serum, plasma, urine, cell media samples and
other biological samples.
KEY FEATURES
Sensitive and accurate. Detection limit of 1 μM and linearity up to 50 μM
D-lactate in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and
reading the fluorescence after 60 min. Room temperature assay.
High-throughput. Can be readily automated as a high-throughput 96-well
plate assay for thousands of samples per day.
KIT CONTENTS
Assay Buffer: 10 mL Enzyme A: 120 μL
NAD Solution: 1 mL Enzyme B: 120 μL
Probe: 750 μL Standard: 1 mL
Storage conditions. The kit is shipped on ice. Store all reagents at -20°C.
Shelf life: 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.
SAMPLE PREPARATION AND CONSIDERATIONS
The following substances interfere and should be avoided in sample
preparation: EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide,
NP-40 (>1%) and Tween-20 (>1%). Samples suspected of having
endogenous L-LDH enzyme activity (e.g. serum, plasma, culture medium
with FBS, etc.) should be deproteinated using a 10 kDa spin filter (e.g.
Microcon YM-10). Deproteinated serum should be diluted 3 × with dH2O.
Samples containing higher than 50 μM pyruvate require an internal

MATERIALS REQUIRED, BUT NOT PROVIDED
Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and
fluorescent plate reader capable of reading at lex/em = 530/585 nm.

datasheet of product :EnzyFluo™ D-Lactate Assay Kit EnzyFluo™ D-Lactate Assay Kit

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