Product name :EnzyFluo™ NAD NADH Assay Kit

Catalog Number :EFND-100

Quantity :100 tests

Price :301 Eur

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Supplier :Bioassays

For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Method: F530/585nm.
Samples: cell, tissue extracts etc.
Species: all.
Procedure: 10 min.
Size: 100 tests.
Detection limit: 0.02 μM.

DESCRIPTION
Pyridine nucleotides play an important role in metabolism and, thus, there is
continual interest in monitoring their concentration levels. Quantitative
determination of NAD+/NADH has applications in research pertaining to
energy transformation and redox state of cells or tissue. BioAssay Systems'
EnzyFluoTM NAD+/NADH assay kit is based on a lactate dehydrogenase
cycling reaction, in which the formed NADH reduces a probe into a highly
fluorescent product. The fluorescence intensity of this product, measured at
lex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the
sample. This assay is highly specific for NAD+/NADH with minimal
interference (<1%) by NADP+/NADPH and is a convenient method to
measure NAD, NADH and their ratio.
APPLICATIONS
Direct Assays: NAD+/NADH concentrations and ratios in cell or tissue
extracts.
KEY FEATURES
Sensitive and accurate. Detection limit of 0.02 μM and linearity up to 1 μM
NAD+/NADH in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and
reading the fluorescence at time zero and 10 min.
High-throughput. Can be readily automated as a high-throughput 96-well
plate assay for thousands of samples per day.
KIT CONTENTS
Assay Buffer: 10 mL Enzyme A: 120 μL
Lactate: 1.5 mL Enzyme B: 120 μL
Probe: 750 μL NAD Standard: 0.5 mL
NAD/NADH Extraction Buffers: each 12 mL
Storage conditions. The kit is shipped on ice. Store all reagents at -20°C.
Shelf life: 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.

MATERIALS REQUIRED, BUT NOT PROVIDED
Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and
fluorescent plate reader capable of reading at lex/em = 530/585 nm.

datasheet of product :EnzyFluo™ NAD NADH Assay Kit EnzyFluo™ NAD NADH Assay Kit

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