Product name :EnzyFluo™ NAD NADH Assay Kit

Catalog Number :EFND-100

Quantity :100 tests

Price :301 Eur

Pay now with :

Supplier :Bioassays

For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Method: F530/585nm.
Samples: cell, tissue extracts etc.
Species: all.
Procedure: 10 min.
Size: 100 tests.
Detection limit: 0.02 μM.

Pyridine nucleotides play an important role in metabolism and, thus, there is
continual interest in monitoring their concentration levels. Quantitative
determination of NAD+/NADH has applications in research pertaining to
energy transformation and redox state of cells or tissue. BioAssay Systems'
EnzyFluoTM NAD+/NADH assay kit is based on a lactate dehydrogenase
cycling reaction, in which the formed NADH reduces a probe into a highly
fluorescent product. The fluorescence intensity of this product, measured at
lex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the
sample. This assay is highly specific for NAD+/NADH with minimal
interference (<1%) by NADP+/NADPH and is a convenient method to
measure NAD, NADH and their ratio.
Direct Assays: NAD+/NADH concentrations and ratios in cell or tissue
Sensitive and accurate. Detection limit of 0.02 μM and linearity up to 1 μM
NAD+/NADH in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and
reading the fluorescence at time zero and 10 min.
High-throughput. Can be readily automated as a high-throughput 96-well
plate assay for thousands of samples per day.
Assay Buffer: 10 mL Enzyme A: 120 μL
Lactate: 1.5 mL Enzyme B: 120 μL
Probe: 750 μL NAD Standard: 0.5 mL
NAD/NADH Extraction Buffers: each 12 mL
Storage conditions. The kit is shipped on ice. Store all reagents at -20°C.
Shelf life: 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.

Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and
fluorescent plate reader capable of reading at lex/em = 530/585 nm.

datasheet of product :EnzyFluo™ NAD NADH Assay Kit EnzyFluo™ NAD NADH Assay Kit

Question about this product?


Email Address


Retype code:

random val Reload Image