Product name :EnzyFluo™ L-Lactate Assay Kit

Catalog Number :EFLLC-100

Quantity :100 tests

Price :285 Eur

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Supplier :Bioassays

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
Method: FL530/585 nm.
Samples: serum, plasma, cell culture media etc.
Species: all.
Procedure: 60 min.
Size: 100 tests.
Detection limit: 1 μM .

LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or
anaerobic conditions. Monitoring lactate levels is, therefore, a good
indicator of the balance between tissue oxygen demand and utilization and
is useful when studying cellular and animal physiology.
Simple, direct and automation-ready procedures for measuring lactate
concentration are very desirable. BioAssay Systems' EnzyFluoTM lactate
assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate,
in which the formed NADH reduces a probe into a highly fluorescent
product. The fluorescence intensity of this product, measured at lex/em =
530/585 nm, is proportional to the lactate concentration in the sample.
Direct Assays: L-lactate in serum, plasma, urine, cell media samples and
other biological samples.
Sensitive and accurate. Detection limit of 1 μM and linearity up to 50 μM Llactate
in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and
reading the fluorescence after 60 min. Room temperature assay.
High-throughput. Can be readily automated as a high-throughput 96-well
plate assay for thousands of samples per day.
Assay Buffer: 10 mL Enzyme A: 120 μL
NAD Solution: 1 mL Enzyme B: 120 μL
Probe: 750 μL Standard: 1 mL
Storage conditions. The kit is shipped on ice. Store all reagents at -20°C.
Shelf life: 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.
Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and
fluorescent plate reader capable of reading at lex/em = 530/585 nm.

datasheet of product :EnzyFluo™ L-Lactate Assay Kit EnzyFluo™ L-Lactate Assay Kit

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