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#28714765   2017/07/17 Save this To Up

Comparison of Estrogen and Progesterone Receptor Antibody Reagents Using Proficiency Testing Data.

- Immunohistochemical analysis of estrogen receptor (ER) and progesterone receptor (PgR) expression in breast cancer is the current standard of care and directly determines therapy. In 2010 the American Society of Clinical Oncology and the College of American Pathologists (ASCO/CAP) published guidelines for ER and PgR predictive testing, encompassing preanalytic, analytic, postanalytic factors; antibody validation; and proficiency testing.

2571 related Products with: Comparison of Estrogen and Progesterone Receptor Antibody Reagents Using Proficiency Testing Data.

Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Progesterone Receptor (Ph Androgen Receptor (Phosph Androgen Receptor (Phosph Estrogen Receptor á (Ab Estrogen Receptor á (Ab Estrogen Receptor á (Ab Estrogen Receptor á (Ab Progesterone Receptor (Ab

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#23894168   2013/11/22 Save this To Up

EP1: a novel rabbit monoclonal antibody for detection of oestrogen receptor α.

Assessment of hormone receptor expression is part of routine examination of every breast cancer. In this study, we report the characterisation of a novel rabbit monoclonal antibody, clone EP1, directed against oestrogen receptor (ER) α. Additionally, its immunohistochemical performance characteristics in archival tissues are evaluated in normal tissues and two distinct cohorts of breast cancer patients.

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MOUSE ANTI BOVINE ROTAVIR Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Progesterone Receptor (Ph Androgen Receptor (Phosph Androgen Receptor (Phosph Glutamate receptor 2 (Pre Interferon-a Receptor Typ MOUSE ANTI BORRELIA BURGD Estrogen Receptor á (Ab

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#20025868   2010/04/02 Save this To Up

Protein and gene expression of estrogen receptor alpha and nuclear morphology of two breast cancer cell lines after different fixation methods.

We assessed morphology and ERalpha protein and gene expression of two breast cancer cell lines after three different fixatives: neutral-buffered 10% formaldehyde, LN-FIX and FineFIX and varying fixation times. We found that the cell morphology was best preserved in cells fixed with LN-FIX. Two commercial fixatives used in this study shrank cells less than formalin. In immunohistochemical assay samples were stained with two different ERalpha antibodies, clone 1D5 and clone SP1. All tested fixatives were suitable for immunohistochemistry. Staining was more intensive and the number of stained cells was larger with the clone 1D5 than with the clone SP1. Our gene expression analysis showed that formalin and LN-FIX preserve the ERalpha better than FineFIX, which is advertised to be optimal for molecular analysis. Our study suggests that tissues fixed with formalin are suitable also for molecular biology assays. This makes possible to research formalin-fixed paraffin-embedded archival tissues also with molecular techniques.

1421 related Products with: Protein and gene expression of estrogen receptor alpha and nuclear morphology of two breast cancer cell lines after different fixation methods.

CAR,Car,Constitutive andr CAR,CAR,Constitutive acti CAR,Car,Constitutive andr Recombinant Human Androge Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Androgen Receptor (Ab 650 Breast cancer membrane pr AZD-3514 Mechanisms: Andr Rabbit anti Estrogen Rece

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#17525635   2007/05/25 Save this To Up

Estrogen receptor antibody incubation time and extent of immunoreactivity in invasive carcinoma of the breast: the importance of optimizing antibody avidity.

We noticed that the percentage and intensity of estrogen receptor (ER) antibody (Ab) AB ER 1D5 immunohistochemical (IHC) staining was altered by Ab incubation time and the type of chromogen detection system in invasive breast carcinomas. We studied the impact of these 2 factors on Ab ER 1D5 immunoreactivity. Serial sections from 22 strongly ER-positive invasive breast carcinomas were immunohistochemically stained with Ab clone ER 1D5 using 3 IHC protocols. One IHC protocol used a 12-hour Ab incubation and a supersensitive, labeled streptavidin-biotin chromogen detection system (12 h-Standard), the second IHC protocol used a 2-hour Ab incubation and a supersensitive, labeled streptavidin-biotin chromogen detection system (2 h-SS), and the third protocol used a 2-hour Ab incubation and a polymer-based detection system (2 h-Env). Twenty identical fields on each slide stained with each IHC protocol were evaluated and staining was quantified using image analysis. The mean staining percentages using the 12 h-Standard, 2 h-SS, and 2 h-Env IHC staining protocols were 89%, 72%, and 47%, respectively (P<0.001). Three of the 22 cases (14%) were ER negative (<10% stained area) with the 2 h-Env IHC protocol. Stain intensity was significantly stronger with the 12 h-Standard Ab incubation IHC protocol than either 2-hour Ab incubation protocol (P<0.001). Twelve cases stained with 2-hour Ab incubation IHC protocols had weak visually seen staining: 7 were Allred total score 2 (ER negative) and 5 were Allred total score 3. Ab ER 1D5 avidity is directly related to factors that impact electrostatic forces, one of which is Ab incubation time. Standard automated stainer Ab incubation times of less than 1 hour may be of insufficient duration and result in artificially low levels of ER immunoreactivity. The chromogen detection system in association with the ER 1D5 Ab also alters levels of immunoreactivity. Optimization of IHC staining protocols should include evaluating the Ab incubation time and chromogen detection system. These factors can substantially alter the extent and intensity of ER IHC staining.

1970 related Products with: Estrogen receptor antibody incubation time and extent of immunoreactivity in invasive carcinoma of the breast: the importance of optimizing antibody avidity.

FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Androgen Receptor (Phosph Androgen Receptor (Phosph

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#16791479   2006/10/25 Save this To Up

Estrogen receptor expression in benign breast ductal cells obtained from random periareolar fine needle aspiration correlates with menopausal status and cytomorphology index score.

Estrogen receptor (ER) expression in breast epithelial cells has potential as a risk marker for development of breast cancer and as a response marker for preventive interventions.

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#16540736   2006/03/16 Save this To Up

Immunohistochemical expression of estrogen receptor in pulmonary adenocarcinoma.

Estrogen receptor (ER) is a useful immunohistochemical marker of breast carcinomas and is commonly used as a means of distinguishing breast carcinomas from adenocarcinomas of other primary sites, including the lung. Previous reports have yielded conflicting data regarding ER immunoreactivity in primary pulmonary adenocarcinomas. In this study the immunohistochemical expression of ER was evaluated in 55 primary lung adenocarcinomas using the 1D5 antibody clone. Immunohistochemistry for thyroid transcription factor-1 (TTF-1), a sensitive and specific marker of pulmonary adenocarcinomas, was also performed. ER expression was observed in 10 (18%) of 55 lung adenocarcinomas. Most of these pulmonary adenocarcinomas showed ER immunoreactivity of weak or moderate intensity (<25% of tumor cell nuclei). However, two cases exhibited strong ER immunoreactivity (51-75% of neoplastic cells). Forty-six (84%) of the 55 lung adenocarcinomas were TTF-1 positive, including all those that expressed ER. These results indicate that a subset of pulmonary adenocarcinomas can exhibit ER immunoreactivity. As such, caution should be exercised in the use of ER immunohistochemistry alone as a means of distinguishing breast carcinomas from lung adenocarcinomas. In the context of an ER-positive lung neoplasm, strong and extensive TTF-1 immunoreactivity can be regarded as strong supportive evidence for a primary bronchogenic adenocarcinoma.

1296 related Products with: Immunohistochemical expression of estrogen receptor in pulmonary adenocarcinoma.

Estrogen Receptor; Clone Estrogen Receptor; Clone Estrogen Receptor; Clone DNA (cytosine 5) methyltr Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Interferon-a Receptor Typ Mouse Anti-Human Interleu Mouse Anti-Human Estrogen Estrogen Receptor á (Ab

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#15979129   2005/08/22 Save this To Up

Expression of cyclooxygenase-2 (COX-2), receptors for estrogen (ER), and progesterone (PR), p53, ki67, and neu protein in endometrial cancer.

We aimed at investigating by immunohistochemistry the relationship between cyclooxygenase-2 (COX-2) and estrogen (ER), and progesterone (PR) receptors in a single institution series of 90 primary untreated endometrial cancer patients. The simultaneous assessment of p53 protein, ki67, and neu protein has been carried out.

2479 related Products with: Expression of cyclooxygenase-2 (COX-2), receptors for estrogen (ER), and progesterone (PR), p53, ki67, and neu protein in endometrial cancer.

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#15722800   2005/02/21 Save this To Up

Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections.

Evaluation of estrogen and progesterone receptors in breast cancer is widely used for the prediction of the response to endocrine therapy and as a biologic parameter closely related to disease prognosis. Immunohistochemistry is considered a specific, sensitive, and economic method for the determination of estrogen receptor/progesterone receptor status. The authors developed the first rabbit antiestrogen receptor monoclonal antibody (clone SP1) used in immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections especially from breast carcinomas. This new antibody, compared with currently available antiestrogen receptor antibodies, has important advantages, including its reactivity even without heat-based antigen retrieval of fixed, embedded tissue sections in immunohistochemistry, and the predominance of nuclear immunostaining with only a very low cytoplasmic signal. A comparative study of immunohistochemistry on 61 histologic specimens from breast cancer cases showed that SP1 yields the same results as the well-known, standardized mouse monoclonal antibody to estrogen receptor (clone 1D5). Antibody affinity of SP1 is 8 times higher than that of 1D5. Thus, SP1 may prove of great value in the assessment of estrogen receptor status in human breast cancer.

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Mouse Anti-Ca19.9 Sialyl Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Pho Estrogen Receptor á (Ab Estrogen Receptor á (Ab Estrogen Receptor á (Ab Estrogen Receptor á (Ab Rabbit Tissue Paraffin Se Shiga Toxin 1 antibody, M Shiga Toxin 2 antibody, M

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#14516320   2003/09/30 Save this To Up

Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas.

A cytometrical image analyzing method for nuclear protein was established using WinROOF, a commercially available, inexpensive software, to determine the status of both estrogen and progesterone receptors. Immunohistochemical evaluation of estrogen receptors (ER) and progesterone receptors (PR) was performed with the anti-ER (clone 1D5) and the anti-PR (clone PgR636), respectively, combined with dextran polymer reagent EnVision+, all of which are approved in vitro diagnostics in Japan. The immunostained results were captured as digital images in Windows, and then analyzed in WinROOF with macroinstructions for analyzing each captured area either immunolabeled with chromogen or counterstained with hematoxylin. This image analysis method graded the immunostained nuclei of carcinoma cells based on staining intensities, and calculated the labeling index (LI) for both ER and PR. Furthermore, the LI correlated highly with the results from a histology score (HSCORE) when 20 breast carcinomas were quantified. Regarding ER, when 20% in the LI was considered as the cut-off point for positive, the positivity of ER in computer-assisted analysis was 75% (15 of 20 cases), and was completely concordant with that of HSCORE-based analysis. These results indicate that the cytometrical image analysis-based quantification could be appropriately applied to the objective determination of the immunohistochemical status of both ER and PR.

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#12789915   2003/06/06 Save this To Up

E-cadherin expression on fine needle aspiration biopsies of breast invasive ductal carcinomas and its relationship to clinicopathologic factors.

To evaluate E-cadherin expression on fine needle aspiration biopsies (FNAB) of breast ductal invasive carcinomas and to correlate that expression with the grade of the tumors, axillary lymph node status, primary tumor size, menopausal status, estrogen-progesterone receptors and Bcl-2 expression.

1646 related Products with: E-cadherin expression on fine needle aspiration biopsies of breast invasive ductal carcinomas and its relationship to clinicopathologic factors.

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