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Anti-Cancer Effects of Ethanol Extract by Inducing Cancer Cell Apoptosis and Cycle Arrest in Prostate Cancer Cells.

To evaluate the potential anti-prostate cancer effects of ethanol extract (PPEE) and its underlying mechanisms.

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Wenyang Huoxue Jiedu formula inhibits thin-cap fibroatheroma plaque formation via the VEGF/VEGFR signaling pathway.

For many years, Guangzhou University of Chinese Medicine has been successfully using the empirical Wenyang Huoxue Jiedu formula (WHJF) to treat coronary heart disease. Modern theories of acute coronary syndrome mainly focus on rupture of thin-cap fibroatheromas (TCFAs), which is closely related to the release of vascular endothelial growth factor and its receptor (VEGF/VEGFR).

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Role of neuronal nitric oxide synthase (nNOS) at medulla in tachycardia induced by repeated administration of ethanol in conscious rats.

Intake of ethanol (alcohol) has been shown to influence cardiovascular function; the underlying brain mechanism remains unclear. Noting that nitric oxide (NO) system in the CNS is involved in the regulation of cardiovascular function, the present study examined the role of NO in medulla in ethanol-induced cardiovascular changes.

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High-throughput extraction and quantification method for targeted metabolomics in murine tissues.

Global metabolomics analyses using body fluids provide valuable results for the understanding and prediction of diseases. However, the mechanism of a disease is often tissue-based and it is advantageous to analyze metabolomic changes directly in the tissue. Metabolomics from tissue samples faces many challenges like tissue collection, homogenization, and metabolite extraction.

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Detection of counterfeit brand spirits using H NMR fingerprints in comparison to sensory analysis.

Beverage fraud involving counterfeiting of brand spirits is an increasing problem not only due to deception of the consumer but also because it poses health risks e.g. from possible methanol admixture. Suspicious spirit samples from Russia and Kenya were analysed using H nuclear magnetic resonance (NMR) spectroscopy in comparison to authentic products. Using linear regression analysis of spectral integral values, 4 counterfeited samples from Russia and 2 from Kenya were easily identifiable with R < 0.7. Sensory analysis using triangle test methodology confirmed significant taste differences between counterfeited and authentic samples but the assessors were unable to correctly identify the counterfeited product in the majority of cases. An important conclusion is that consumers cannot assumed to be self-responsible when consuming counterfeit alcohol because there is no general ability to organoleptically detect counterfeit alcohol.

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The Room-Temperature Chemiresistive Properties of Potassium Titanate Whiskers versus Organic Vapors.

The development of portable gas-sensing units implies a special care of their power efficiency, which is often approached by operation at room temperature. This issue primarily appeals to a choice of suitable materials whose functional properties are sensitive toward gas vapors at these conditions. While the gas sensitivity is nowadays advanced by employing the materials at nano-dimensional domain, the room temperature operation might be targeted via the application of layered solid-state electrolytes, like titanates. Here, we report gas-sensitive properties of potassium titanate whiskers, which are placed over a multielectrode chip by drop casting from suspension to yield a matrix mono-layer of varied density. The material synthesis conditions are straightforward both to get stable single-crystalline quasi-one-dimensional whiskers with a great extent of potassium replacement and to favor the increase of specific surface area of the structures. The whisker layer is found to be sensitive towards volatile organic compounds (ethanol, isopropanol, acetone) in the mixture with air at room temperature. The vapor identification is obtained via processing the vector signal generated by sensor array of the multielectrode chip with the help of pattern recognition algorithms.

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Cytotoxic Activities of Fractions of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes), and the Induction of Cell Cycle Arrest and Apoptosis in MGC-803 Cells.

Phellinus igniarius, a perennial medicinal mushroom, has been used in China and other countries of East Asia for the treatment of various diseases, including cancer. The purpose of this study is to evaluate the cytotoxic activities of different fractions of an ethanol extract from Ph. igniarius and to elucidate a possible antitumor mechanism. An ethanol extract of Ph. igniarius was partitioned into a petroleum ether fraction, an ethyl acetate fraction (EAF), an n-butanol fraction, and a water-soluble fraction. The cytotoxic activity of all the fractions was initially screened in a brine shrimp lethality test, then evaluated by the Cell Counting Kit-8 assay against 5 human tumor cell lines: MGC-803, BEL-7402, HeLa, MCF-7, and HCT-116. The cell cycle distribution induced by EAF on MGC-803 cells was analyzed by flow cytometry with propidium iodide staining, and apoptosis was determined using flow cytometry with Annexin V/propidium iodide staining. The results of the brine shrimp lethality test and the Cell Counting Kit-8 assay showed that EAF was the most active fraction, displaying strong inhibitory activity against the MGC-803, BEL-7402, and MCF-7 cancer cell lines. Flow cytometry analysis indicated that EAF could induce S-phase cell cycle arrest in MGC-803 cells and cause apoptosis in a concentration-dependent manner. This study demonstrated that EAF, as the most active fraction of Ph. igniarius, exerted antitumor activity by inducing MGC-803 cell apoptosis via S-phase cell cycle arrest.

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Measurement uncertainty of blood ethanol concentration in drink-driving cases in an emergency laboratory.

The quality of blood ethanol concentration (BEC) determination is important because of its legal ramifications. Measurement uncertainty provides quantitative information about the quality and reliability of test results. In this study, we aim to calculate the measurement uncertainty for the ethanol test in our laboratory measured with a Synchron Systems Ethanol assay kit by employing an enzymatic rate method on the Beckman-Coulter Olympus AU400 auto analyzer (Beckman Coulter Inc, Melville, USA).

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Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats.

To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage.

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Protective Effect of Combined Caffeic Acid Phenethyl Ester and Bevacizumab Against Hydrogen Peroxide-Induced Oxidative Stress in Human RPE Cells.

This study aimed to evaluate the protective effects of caffeic acid phenethyl ester (CAPE) and combined CAPE-bevacizumab against oxidative stress induced by hydrogen peroxide (HO) in human retinal pigment epithelium.

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