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           Search results for: Evaluation of three tests for the detection of rabbit haemorrhagic disease virus in wild rabbits.   

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#22943557   2012/11/09 Save this To Up

A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies.

Antibodies to non-pathogenic rabbit caliciviruses (RCVs) cross-react in serological tests for rabbit hemorrhagic disease virus (RHDV) and vice versa, making epidemiological studies very difficult where both viruses occur. It is important to understand the distribution and interaction of the two viruses because the highly pathogenic RHDV has been used as a biocontrol agent for wild rabbits in Australia and New Zealand for the past 17 years. The presence of the benign RCV Australia 1 (RCV-A1) is considered a key factor for the failure of RHDV mediated rabbit control in cooler, wetter areas of Australia.

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#9881440   1999/03/16 Save this To Up

Evaluation of three tests for the detection of rabbit haemorrhagic disease virus in wild rabbits.

Two double antibody sandwich ELISA kits and the immunoblot were evaluated for the detection of rabbit haemorrhagic disease (RHD) virus in wild rabbits. Either liver alone or liver and spleen separately or a pool of liver and spleen tissues from 106 wild rabbits were tested in all three tests. They produced very similar results, except that ELISA kit A gave three doubtful results which were confirmed as negative by retesting in the same test and by the immunoblot and ELISA kit B. Both ELISA kits can be used for the rapid detection of acute RHD, and ELISA kit A can detect both acute and chronic RHD virus infection. The immunoblot is useful as a confirmatory test when ELISA-positive results do not correlate with gross and/or histopathological findings.

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