Only in Titles

           Search results for: Expression Systems    

paperclip

#32101749   // Save this To Up

Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s.

Group 2 innate lymphoid cells (ILC2s) are abundant in non-lymphoid tissues and increase following infectious and inflammatory insults. In solid tumors, however, ILC2s constitute a relatively small proportion of immune cells. Here, we show, using melanoma as a model, that while the IL-33/IL C2/eosinophil axis suppresses tumor growth, tumor-derived lactate attenuates the function and survival of ILC2s. Melanomas with reduced lactate production (LDHA) are growth delayed and typified by an increased number of ILC2s compared with control tumors. Upon IL-33 stimulation, ILC2s accompanied by eosinophils more effectively restrain the growth of LDHA tumors than control melanomas. Furthermore, database analysis reveals a negative correlation between the expression of LDHA and markers associated with ILC2s and the association of high expression of IL33 and an eosinophil marker SIGLEC8 with better overall survival in human cutaneous melanoma patients. This work demonstrates that the balance between the IL-33/ILC2/eosinophil axis and lactate production by tumor cells regulates melanoma growth.

2822 related Products with: Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s.

D ( ) Lactic acid lithium LIVER DISEASES Total Bile Multiple organ tumor tiss DL-Lactic acid CAS: [598- FDA Standard Frozen Tissu Human Dnak (HSP70) His ta 2 Tolylboronic acid CAS N DL Lactic acid lithium sa p Toluenesulfonic acid mo EnzyChrom™ D-Lactate As Di p toluoyl L tartaric a Tissue array of ovarian g

Related Pathways

paperclip

#32101716   // Save this To Up

Probing Mechanisms of Transcription Elongation Through Cell-to-Cell Variability of RNA Polymerase.

The process of transcription initiation and elongation are primary points of control in the regulation of gene expression. Although biochemical studies have uncovered the mechanisms involved in controlling transcription at each step, how these mechanisms manifest in vivo at the level of individual genes is still unclear. Recent experimental advances have enabled single-cell measurements of RNA polymerase (RNAP) molecules engaged in the process of transcribing a gene of interest. In this article, we use Gillespie simulations to show that measurements of cell-to-cell variability of RNAP numbers and interpolymerase distances can reveal the prevailing mode of regulation of a given gene. Mechanisms of regulation at each step, from initiation to elongation dynamics, produce qualitatively distinct signatures, which can further be used to discern between them. Most intriguingly, depending on the initiation kinetics, stochastic elongation can either enhance or suppress cell-to-cell variability at the RNAP level. To demonstrate the value of this framework, we analyze RNAP number distribution data for ribosomal genes in Saccharomyces cerevisiae from three previously published studies and show that this approach provides crucial mechanistic insights into the transcriptional regulation of these genes.

1979 related Products with: Probing Mechanisms of Transcription Elongation Through Cell-to-Cell Variability of RNA Polymerase.

Mouse Anti-Human Prolifer CD23, B-Cell; Clone MHM6 Cell Meter™ Live Cell C Human Mouse Rat Phospho-p Cell Meter™ Caspase 3 7 Rat anti mouse CD205 (a d Cultrex In Vitro Angiogen Lung large cell carcinoma High density (208 core) t Nycodenz, non ionic, non DirectPCR Lysis Reagent ( Hairy Cell Leukemia; Clo

Related Pathways

paperclip

#32101569   // Save this To Up

Dop1R1, a type 1 dopaminergic receptor expressed in Mushroom Bodies, modulates Drosophila larval locomotion.

As in vertebrates, dopaminergic neural systems are key regulators of motor programs in insects, including the fly Drosophila melanogaster. Dopaminergic systems innervate the Mushroom Bodies (MB), an important association area in the insect brain primarily associated to olfactory learning and memory, but that has been also implicated with the execution of motor programs. The main objectives of this work is to assess the idea that dopaminergic systems contribute to the execution of motor programs in Drosophila larvae, and then, to evaluate the contribution of specific dopaminergic receptors expressed in MB to these programs. Our results show that animals bearing a mutation in the dopamine transporter show reduced locomotion, while mutants for the dopaminergic biosynthetic enzymes or the dopamine receptor Dop1R1 exhibit increased locomotion. Pan-neuronal expression of an RNAi for the Dop1R1 confirmed these results. Further studies show that animals expressing the RNAi for Dop1R1 in the entire MB neuronal population or only in the MB γ-lobe forming neurons, exhibit an increased motor output, as well. Interestingly, our results also suggest that other dopaminergic receptors do not contribute to larval motor behavior. Thus, our data support the proposition that CNS dopamine systems innervating MB neurons modulate larval locomotion and that Dop1R1 mediates this effect.

2035 related Products with: Dop1R1, a type 1 dopaminergic receptor expressed in Mushroom Bodies, modulates Drosophila larval locomotion.

Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep

Related Pathways

paperclip

#32101077   // Save this To Up

IPA-1 a putative chromatin remodeler/helicase-related protein of plays important roles in antibiosis against and induction of systemic disease resistance.

spp. are filamentous fungi that colonize plant roots conferring beneficial effects to plants, indirectly through the induction of their defense systems or directly through the suppression of phytopathogens in the rhizosphere. Transcriptomic analyses of emerged as a powerful method for identifying the molecular events underlying the establishment of this beneficial relationship. Here, we focus on the transcriptomic response of during its interaction with . The main response of to co-cultivation with was the repression of gene expression. The biological processes of transport and metabolism of carbohydrates were downregulated, including a set of cell-wall-degrading enzymes putatively relevant for root-colonization. Repression of such genes reached their basal levels at later times of the interaction when genes belonging to the biological process of copper ion transport were induced, a necessary process providing copper as a cofactor for cell-wall degrading enzymes with auxiliary activities class. RNA-Seq analyses showed the induction of a member of the SNF2 family of chromatin remodelers/helicase-related proteins, which was named IPA-1 (Increased Protection of Arabidopsis-1). Sequence analyses of IPA-1 showed as its closest relatives members of the Rad5/Rad16- and SNF2-subfamilies; however, it grouped into a different clade. Although deletion of in did not affect its growth, the antibiosis of Δ culture filtrates showed a diminished effect against but remained unaltered against Triggering of the plant defense genes in plants treated with Δ was higher, showing enhanced resistance against but not against Botrytis.

1074 related Products with: IPA-1 a putative chromatin remodeler/helicase-related protein of plays important roles in antibiosis against and induction of systemic disease resistance.

Rabbit Anti-Cell death in Anti-ACIN1ACIN1(Apoptotic Tubby-related protein 1 a Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-APIP Apaf1 In Rabbit Anti-G protein alp Polyclonal Antibody Recep Apoptosis Phospho-Specifi Rabbit Anti-APIP Apaf1 In Monoclonal Anti Breast Ca

Related Pathways

paperclip

#32100907   // Save this To Up

Differential gene expression changes in the dorsal root versus trigeminal ganglia following peripheral nerve injury in rats.

The dorsal root (DRG) and trigeminal (TG) ganglia contain cell bodies of sensory neurons of spinal and trigeminal systems, respectively. They are homologs of each other; however, differences in how the two systems respond to injury exist. Trigeminal nerve injuries rarely result in chronic neuropathic pain (NP). To date, no genes involved in the differential response to nerve injury between the two systems have been identified. We examined transcriptional changes involved in the development of trigeminal and spinal NP.

2862 related Products with: Differential gene expression changes in the dorsal root versus trigeminal ganglia following peripheral nerve injury in rats.

DNA (cytosine 5) methyltr Mouse Epstein-Barr Virus Human Kidney injury molec FDA Standard Frozen Tissu FDA Standard Frozen Tissu Rat TGF-beta-inducible ea FDA Standard Frozen Tissu pCAMBIA0105.1R Vector, (G FDA Standard Frozen Tissu FDA Standard Frozen Tissu Rat TGF-beta-inducible ea MultiGene Gradient therm

Related Pathways

paperclip

#32100723   // Save this To Up

Successful aging: insights from proteome analyses of healthy centenarians.

Healthy aging depends on a complex gene-environment network that is ultimately reflected in the expression of different proteins. We aimed to perform a comparative analysis of the plasma proteome of healthy centenarians (n=9, 5 women, age range 100-103 years) with a notably preserved ambulatory capacity (as a paradigm of 'successful' aging), and control individuals who died from a major age-related disease before the expected life expectancy (n=9, 5 women, age range: 67-81 years), and while having impaired ambulatory capacity (as a paradigm of 'unsuccessful' aging). We found that the expression of 49 proteins and 86 pathways differed between the two groups. Overall, healthy centenarians presented with distinct expression of proteins/pathways that reflect a healthy immune function, including a lower pro-inflammatory status (less 'inflammaging' and autoimmunity) and a preserved humoral immune response (increased B cell-mediated immune response). Compared with controls, healthy centenarians also presented with a higher expression of proteins involved in angiogenesis and related to enhanced intercellular junctions, as well as a lower expression of proteins involved in cardiovascular abnormalities. The identification of these proteins/pathways might provide new insights into the biological mechanisms underlying the paradigm of healthy aging.

1751 related Products with: Successful aging: insights from proteome analyses of healthy centenarians.

Anti 3 DG imidazolone Mon Ofloxacin CAS Number [824

Related Pathways