Only in Titles

           Search results for: Free Fatty Acid Quantification Kit   

paperclip

#28260110   2017/03/05 Save this To Up

Inhibitory effect of emodin on fatty acid synthase, colon cancer proliferation and apoptosis.

Fatty acid synthase (FASN) is a key anabolic enzyme for de novo fatty acid synthesis, which is important in the development of colon carcinoma. The high expression of FASN is considered a promising molecular target for colon cancer therapy. Emodin, a naturally occurring anthraquinone, exhibits an anticancer effect in various types of human cancer, including colon cancer; however, the molecular mechanisms remain to be fully elucidated. Cell viability was evaluated using a Cell Counting Kit‑8 assay. The apoptosis rate of cells was quantified via flow cytometry following Annexin V/propidium iodide staining. FASN activity was measured by monitoring oxidation of nicotinamide adenine dinucleotide phosphate at a wavelength of 340 nm, and intracellular free fatty acid levels were detected using a Free Fatty Acid Quantification kit. Western blot analysis and reverse transcription‑polymerase chain reaction were used to detect target gene and protein expression. The present study was performed to investigate whether the gene expression of FASN and its enzymatic activity are regulated by emodin in a human colon cancer cell line. Emodin markedly inhibited the proliferation of HCT116 cells and a higher protein level of FASN was expressed, compared with that in SW480, SNU-C2A or SNU‑C5 cells. Emodin significantly downregulated the protein expression of FASN in HCT116 cells, which was caused by protein degradation due to elevated protein ubiquitination. Emodin also inhibited intracellular FASN enzymatic activity and reduced the levels of intracellular free fatty acids. Emodin enhanced antiproliferation and apoptosis in a dose‑ and time‑dependent manner. The combined treatment of emodin and cerulenin, a commercial FASN inhibitor, had an additive effect on these activities. Palmitate, the final product of the FASN reaction, rescued emodin‑induced viability and apoptosis. In addition, emodin altered FASN‑involved signaling pathways, including phosphatidylinositol 3-kinase/Akt and mitogen‑activated protein kinases/extracellular signal-regulated kinases 1/2. These results suggested that emodin-regulated cell growth and apoptosis were mediated by inhibiting FASN and provide a molecular basis for colon cancer therapy.

1222 related Products with: Inhibitory effect of emodin on fatty acid synthase, colon cancer proliferation and apoptosis.

Fatty Acid Synthase (FASN Fatty Acid Synthase antib Fatty Acid Synthase antib Fatty acid free heat sho Fatty acid free heat sho Fatty acid free heat sho Fatty acid free heat sho serologically defined col Triglyceride Assay Kit Li Androst-4-ene-3,17-dion-1 Cancer Apoptosis Phospho- BSA, Fatty Acid-Free(Assa

Related Pathways

paperclip

#15047469   2004/03/29 Save this To Up

[Value of non-esterified fatty acids quantification in diabetes].

Non-esterified fatty acids (NEFA) play a complex role in glucidic homeostasis. This role led us to investigate their quantification in diabetic patients. The plasmatic NEFA concentrations, measured with the FA115 kit of Randox, showed significant differences between control patients (0.42 +/- 0.14 mmol/L, N = 50) and diabetic patients (0.68 +/- 0.35 mmol/L, p < 0.01, 443 diabetic patients (70 with type l and 373 with type 2 diabetes)). NEFA concentrations were significantly higher in type 2 diabetics (0.70 +/- 0.32 mmol/L) when compared to type 1 diabetics (0.59 +/- 0.35 mmol/L, p < 0.05). In type 2 diabetics, a significant correlation was observed between NEFA and glucose concentrations at 8 hrs a.m., and the mean glucose concentrations along the day (p < 0.001). In contrast NEFA concentrations were less correlated to levels of HbA1c. NEFA were well correlated with cholesterol and triglycerides (p < 0.05) but not with Lp(a). They were also correlated with BMI but not with age or duration of the disease. Diabetic patients on metformin associated to lipolytic treatment, presented lower concentrations of NEFA and better glucidic control. The results confirm the role of NEFA in glucidic homeostasis and suggest an interest for their routine determination.

1905 related Products with: [Value of non-esterified fatty acids quantification in diabetes].

Triglyceride Assay Kit Li Leptin ELISA Kit, Rat Lep Anti 3 DG imidazolone Mon Rat intestinal fatty acid Free Fatty Acid Quantific Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Fatty acid free heat sho Fatty acid free heat sho Fatty acid free heat sho Fatty acid free heat sho Sterile filtered goat se

Related Pathways

paperclip

#2582621   1989/12/26 Save this To Up

A rapid, automated micromethod for measuring free fatty acids in plasma/serum.

The investigation of mobilization and utilization of fat as fuel in human subjects requires the quantification of free fatty acids (FFA) in the circulating plasma. Methods in current use involve tedious extraction and titration, or enzymatic reaction with colorimetric or fluorometric detections. A rapid and reliable micro-technique is needed. We have adapted the manual enzymatic method of the Wako NEFAC kit to an automated rapid assay performed with a micro-centrifugal analyzer. This method depends upon the specific acylation of CoA by FFA, followed by oxidation and condensation to form a purple adduct measurable at 550 nm. The acylation step requires incubation at 37 degrees C for 10 min, a critical step for serum/plasma analysis. Only 4 microL of plasma is needed, and 20 tests can be performed in 20 min. The precision (CV) of sample analysis is within 2%. The results for the samples analyzed by this technique are within 4% (SEM 1%) of results by the manual method. Thus accurate results are achieved at reduced cost, time, and sample size.

2557 related Products with: A rapid, automated micromethod for measuring free fatty acids in plasma/serum.

Triglyceride Assay Kit Li Human interleukin 2(IL-2) Fatty acid free heat sho Fatty acid free heat sho Fatty acid free heat sho Fatty acid free heat sho Bovine Serum Albumin (BSA BSA, Fatty Acid-Free(Assa BSA, Fatty Acid-Free (Ass BSA, Fatty Acid-Free(Assa Fatty acid free heat shoc Fatty acid free heat shoc

Related Pathways