Search results for: Giemsa Stock Solution
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Giemsa as a fluorescent dye for mineralizing bone-like nodules in vitro.Giemsa was first used as a fluorescent dye for mineralized bone and cartilage in tissue sections. The aim of this study was to establish the use of Giemsa as a fluorescent dye for mineralizing bone-like nodules produced in cell cultures. Osteoblasts were grown under mineralizing conditions for 14 days, producing typical bone-like nodules. Upon staining with Giemsa stock solution for 1 min, the mineralizing nodules could be selectively visualized emitting intense green and red fluorescence when observed under blue and green illumination, respectively. The textural details of the nodules were clearly observed under fluorescence microscopy, allowing to identify regions with different degrees of mineralization. The mineralized nature of the nodules was confirmed using von Kossa's method, Alizarin Red S staining and x-ray mapping for Ca and P in a scanning electron microscope, showing a strong correlation between the mineralizing and the fluorescent nodules. The selective fluorescence was related to the mineral phase, being absent in decalcified samples. The use of Giemsa as a fluorescent dye for mineralizing bone-like nodules presents a simple alternative method to quickly analyze biomineralization assays in vitro under fluorescence microscopy, particularly in the biological evaluation of biomaterials.
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[In vitro susceptibility of P. falciparum isolates from Abidjan (Côte d'Ivoire) to quinine, artesunate and chloroquine].Malaria is still a major public health problem in Côte d'Ivoire. Both treatment and control there are hampered by the spread of resistance to common antimalarial drugs, especially in the south where multidrug-resistant malaria is highly prevalent. Recent treatment guidelines require in vitro tests and the adaptation of drug policies according to local resistance rates. In addition to performing clinical assays in the field, we sought to establish a national map of drug resistance by using in vitro tests with clinical surveys. These make it possible to detect changes in susceptibility and are expected to prevent the emergence of resistance against the most recently introduced combined therapy.
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Optimising FNA processing--a collection fluid allowing Giemsa, PAP and H & E staining, and facilitating thinprep, cytospin and direct smears and ancillary tests.
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[Modification of a panoptic method of staining isolated cells].A simple and rapid method of isolated cell staining was achieved by modification of the Pappenheim's method. It is based on the following steps: (1) dilution of commonly used stock solutions May-Grünwald (1:1), Giemsa-Romanowsky (1.25% v/v), (2) reverse sequence of the used staining solutions in comparison with the original method, (3) shorter time of procedure (5 min/slide), (4) utilization of distilled water of pH 5.1-5.6 for dilution of solutions and rinsing of slides, (5) it is not necessary to add serum to cell suspensions. The presented histological method is suitable for cytomorphological examination of cells. It can be applied in routine hematological laboratories not equipped with cytospins. (Fig. 5, Ref. 6.).
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Stability study of Azure B, Eosin Y and commercial Romanowsky Giemsa stock solutions using high performance liquid chromatography.The stability of Azure B and Eosin Y in stock solutions of the individual compounds as well as in mixtures of the two dyes was studied. The purpose of the study of these two essential constituents of the Romanowsky Giemsa stain, commonly used in cytology and histology, was to select a stable mixture as a definitive stock solution. Two specific high performance liquid chromatographic methods were used to monitor qualitative and quantitative changes in solutions. Several parameters influencing the stability of Azure B were examined e.g. the type of counter ion, the presence of Eosin Y and the type of solvent used. The second part focused on the stability of Eosin Y in mixtures with different cationic dyes submitted to high temperatures. In conclusion, an Azure B SCN-Eosin Y acid mixture in dimethylsulphoxide (concentrations 0.75% and 0.12%, respectively) was selected as being the most appropriate composition of a stock solution for the Romanowsky Giemsa stain.
2892 related Products with: Stability study of Azure B, Eosin Y and commercial Romanowsky Giemsa stock solutions using high performance liquid chromatography.Giemsa Stock Solution Giemsa Stock Solution Giemsa Stock Solution Caspase-1 Inhibitor Z-YVA Caspase-1 Inhibitor Z-YVA Caspase-1 Substrate YVAD- Caspase-1 Substrate YVAD- Caspase-1 Substrate YVAD- Caspase-1 Substrate YVAD- Z 366 High Performance Ce Red Load Taq Master high Red Load Taq Master high
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The standard Romanowsky-Giemsa stain in histology.A new and technically simple Romanowsky-Giemsa (RG) stain is proposed as a standardized technique for use in histology. An RG stock solution (pure azure B 7.5 g/l, eosin Y as eosinic acid 1.2 g/l in dimethylsulfoxide) is diluted to form the working solution with HEPES-buffer, pH 6. Staining time is 30-90 min after formol-calcium solution (or 2-4 hr after formaldehyde-organic acid mixtures). The resulting overstained sections are to be differentiated. A tannic acid-acetic acid combination in an isopropanol-water mixture was found to give optimum results within 100 sec. Subsequent dehydration is in isopropanol only. The staining pattern obtained is polychrome. The distribution of colors in detail is influenced by the modes of pre- and posttreatment. Of practical interest is the development of green and greenish blue colors on collagen fibrils which contrast strongly against the pink of sarcoplasm. For this and other reasons, this RG stain version seems suitable to replace the trichrome Gomori-type trichrome stains under appropriate processing conditions.
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Quality control of azure B preparations by liquid chromatography and standardization with azure B tetrafluoroborate.A liquid chromatographic procedure for the quantitative determination of the thiazine dye azure B, the principal constituent of Romanowsky stains, is presented. Unlike previous methods relying on peak area normalization, the present approach involves real quantitation through calibration with the reference standard azure B tetrafluoroborate. The method has been used for the quality control of commercial azure B preparations and to study their stability in stock and staining solutions, either or not in the presence of eosin Y. Results suggest that highly pure azure B perchlorate meets the requirements of a reference material, useful for standardization of Romanowsky-Giemsa staining in haematology.
2652 related Products with: Quality control of azure B preparations by liquid chromatography and standardization with azure B tetrafluoroborate.Biotin-IETD-FMK; Appearan Biotin-VAD-FMK ; Appearan Biotin-DEVD-FMK; Appearan RIPA Buffer; Appearance L RIPA Buffer; Appearance L Leptin ELISA Kit, Rat Lep PARP Blocking Peptide ;Ap Alpha- Amylase Blocking P ILP-2 Blocking Peptide;Ap PUMA bbc3 Blocking Peptid p53DIN1 Blocking Peptide; Oct-1 Blocking Peptide;Ap
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Fully automated preparation of high-quality stained blood films.A detailed description is given of the operation of Technicon's AutoSlide, which automatically produces a microscope-ready, precipitate-free, stained blood smear with superior cell distribution and good morphology. A 40-test-tube turntable carrying anticoagulated blood inputs samples at 40-second intervals. The blood films are drawn consecutively on a continuous Mylar substrate, with nylon mesh replacing the usual glass slide spreaders. This flexible substrate then passes through drying, fixing, staining and final drying stations. The methanol of conventional Romanowsky stains is replaced by low-volatility solvents. The fixing solution contains solvents, toluidine blue O, glutaraldehyde and a trace of water. The modified Giemsa-stain stock, when mixed with buffer, remains precipitate free for several days. Finally the blood film is imprinted with a date and identification number. Liquid monomer is dispensed onto each stained blood film, followed by a microscope slide. The monomer is then polymerized using ultraviolet light. Permanent transfer of the stained and labeled blood film occurs when the Mylar is stripped from the slide. The usable area for examination is approximately five times larger than that of a typical manual wedge smear.
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Staining properties and stability of a standardised Romanowsky stain.An evaluation of the standardised Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-Grünwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. The stability of the stock stain solution and of the 'working' stain (stock + buffer) has been studied by, respectively, thin-layer chromatography and visible spectroscopy. No change was detected in the composition of the stock solution at ambient temperature over a period of six months. Stability was unaffected by the composition of the container (polyethylene, PyrexTM, or soda-glass) or by daylight. The 'working' solution was stable for 3 hours. Thereafter a precipitate is formed, consisting of thiazine dyes and eosin in a molar ratio of approximately 2:1.
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[Can azur-B eosin replace the May-Grünwald-Giemsa stain?].A new method is described for staining blood and bone marrow smears. It is characterized by the presence of only two dyes, purified azure B and eosin in methanol, as stock solutions. Staining results are equivalent to those obtained by using the traditional dye mixtures according to May and Grunwald, Giemsa, Leishman or Wright. Different from these azure B-eosin staining can be standardized and is easier to be handled. Correlations between the azure-B-eosin and May-Grunwald-Giemsa (MGG) staining methods are briefly discussed.
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