Search results for: Glutathione Reductase Assay Kit
#28820276 2017/08/18 Save this To Up
Attenuation of Sulfite-Induced Testicular Injury in Rats by Zingiber officinale Roscoe.Sulfite salts, including sodium metabisulfte, are widely used as preservatives in foods and pharmaceutical agents. Previous studies suggest that oxidative stress may be an important mediator of testicular injury. The present study was designed to elucidate the effect of exposure to sodium metabisulfite by gavage without or with Zingiber officinale (ginger) extract on the rat testes. Thirty-two male Wistar rats were randomly divided into control, ginger-treated (500 mg/kg/day), sodium metabisulfite- (SMB-) treated (260 mg/kg/day), and SMB + ginger- (SZ-) treated groups. After 28 days, the rats were anesthetized by ether and, after laparotomy, blood was collected from the heart to determine testosterone level by the enzyme-linked immunosorbent assay (ELISA) kit. Then left testes and cauda epididymis of all animals were removed for histological examination and sperm analysis, and right testes were removed for assessing lipid peroxidation (indexed by malondialdehyde [MDA]) and antioxidant enzymes. The results showed that spermatogenesis, epididymal morphometry, and sperm parameters were affected by SMB. There was a significant increase in MDA level and a significant reduction in the activities of glutathione peroxidase (GPx), glutathione reductase (GR), and catalase (CAT) in the SMB-treated rats compared to the control. Ginger treatment of SMB-exposed rats significantly increased testosterone level and the number of different spermatogenic cells. The level of MDA reversed to the control levels and the activities of GPx and GR were significantly increased when SMB was coadministered with ginger extract. It is concluded that coadministration of ginger, through its antioxidant and androgenic properties, exerts a protective effect against SMB-induced testicular oxidative stress.
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#26767257 2016/01/15 Save this To Up
[Study on immunologic function of thioredoxin glutathione reductase from Schistosoma japonicum].To study the immunogenicity and the immuno-protection of thioredoxin glutathione reductase from Schistosomajaponicum (SjTGR) against schistosome infection in mice.
2434 related Products with: [Study on immunologic function of thioredoxin glutathione reductase from Schistosoma japonicum].Glutathione Reductase Ass HT Glutathione Reductase Goat Anti-Human Thioredox Glutathione Reductase Flu Glutathione Reductase Act Glutathione Reductase Ass Glutathione Reductase Ass Thioredoxin Reductase Ass anti-Thioredoxin Reductas anti-Thioredoxin Reductas anti-Glutathione Reductas anti-Thioredoxin Reductas
#25261820 2014/12/02 Save this To Up
Copper-induced changes in intracellular thiols in two marine diatoms: Phaeodactylum tricornutum and Ceratoneis closterium.Phytochelatins and glutathione (reduced (GSH) and oxidised (GSSG)) are important intracellular ligands involved in metal sequestration and detoxification in algae. Intracellular ratios of GSH:GSSG are sensitive indicators of metal stress in algae, and like phytochelatin production are influenced by metal speciation, concentration, exposure time and the biological species. This study investigated the effect of copper exposure on phytochelatin and glutathione content in two marine diatoms Phaeodactylum tricornutum and Ceratoneis closterium at various time intervals between 0.5 and 72h. Liberation of cellular glutathione and phytochelatins was optimised using freeze/thaw cycles and chemical extraction, respectively. Extracted phytochelatins were derivatised (by fluorescent tagging of thiol compounds), separated and quantified using HPLC with fluorescence detection. Glutathione ratios were determined using a commercially available kit, which uses the enzyme glutathione reductase to measure total and oxidised glutathione. Despite similarities in size and shape between the two diatoms, differences in internalised copper, phytochelatin production (both chain length and quantity) and reduced glutathione concentrations were observed. P. tricornutum maintained reduced glutathione at between 58 and 80% of total glutathione levels at all time points, which would indicate low cellular stress. In C. closterium reduced glutathione constituted <10% of total glutathione after 48h. P. tricornutum also produced more phytochelatins and phytochelatins of longer chain length than C. closterium despite the latter species internalising significantly more copper.
2696 related Products with: Copper-induced changes in intracellular thiols in two marine diatoms: Phaeodactylum tricornutum and Ceratoneis closterium.Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Human Epstein-Barr Virus Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Amplite™ Intracellular Mouse Epstein-Barr Virus TGF beta induced factor 2
#25195052 2015/02/03 Save this To Up
Expression changes of antioxidant, apoptotic, anti-apoptotic genes and miR-15b-34a-21-98 in over tissue by using erythromycin, quinacrine and tetracycline in non-surgical sterilization.In the present study, effects on expression of antioxidant, apoptotic and anti-apoptotic genes (GSR, GRX3, SOD1, RAI-NOS, HSP7, BAX, Bcl-2, CASP3 and MDH1) of substances being used in non-surgical sterilization such as quinacrine, erythromycin and tetracycline were evaluated in over tissue. Moreover, expression of some specific mi-RNA (miR-15b, miR-21, miR34a and miR-98) that playing a role in apoptosis was determined in same tissue. Prospective comparative experimental study. Genetics and Histology laboratory. Total number of 28 Wistar albino 12-14 week old female rats with regular cycles and 200-220 grams in weight. Total RNA was isolated from tissues by using a RNA isolation kit. Gene expression levels were evaluated by Real-Time PCR method. Tubal passage and fibrosis induction in tissues was observed in the histochemical analysis. In the statistical analysis of data Kruskal-Wallis variance analysis and Mann-Whitney U test were used and p < 0.05 were accepted as significant. While the expressions of target genes found to be increased in quinacrine and erythromycin group when compared to control group, this increase was insignificant. In quinacrine group, increase in the SOD1 expression levels was only statistically significant (p < 0.05). Expression levels of miR-15b, miR-21, miR34a and miR-98 microRNAs were found to be up-regulated in all experimental groups, despite this, only the increased expression miR-34 was found as statistically significant when compared to control. Tubal blockage and fibrosis induction scores of quinacrine, erythromycin and tetracycline were significantly higher than control. Results of the present study suggest that the doses treated of quinacrine, erythromycin and tetracycline used in non-surgical sterilization effect poorly the expression of anti-oxidant, apoptotic and anti-apoptotic genes, but the expression of miR-34 playing the role in apoptosis increased after treatment of these substances.
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#24149024 2014/01/17 Save this To Up
A bioactive probe for glutathione-dependent antioxidant capacity in breast cancer patients: implications in measuring biological effects of arsenic compounds.Glutathione, a major cellular non-protein thiol (NPSH), serves a central role in repairing damage induced by cancer drugs, pollutants and radiation and in the detoxification of several cancer chemotherapeutic drugs and toxins. Current methods measure glutathione levels only, which require cellular extraction, rather than the glutathione recycling dependent antioxidant activity in intact cells. Here, we present a novel method using a bioactive probe of the oxidative pentose phosphate cycle, termed the OxPhos™ test, to quantify glutathione recycling dependent antioxidant activity in whole blood and intact human and rodent cells without the need for the isolation and cytoplasm extraction of cells.
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#21999191 2011/11/14 Save this To Up
Rapamycin reduces reactive oxygen species in cultured human corneal endothelial cells.To investigate the protective effect of rapamycin on oxidative stress-induced cell death of human corneal endothelial cells (HCECs).
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#21950696 2011/09/28 Save this To Up
Effect of cysteamine on oxidative stress-induced cell death of human corneal endothelial cells.The principal objective of this study was to evaluate the protective effect of cysteamine against the oxidative stress-induced cell death of human corneal endothelial cells.
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#19394604 2010/07/08 Save this To Up
Cigarette smoking affects specific sperm oxidative defenses but does not cause oxidative DNA damage in infertile men.To evaluate the effects of tobacco consumption on the oxidative defenses of sperm, the glutathione system (GS), and sperm DNA oxidation.
2111 related Products with: Cigarette smoking affects specific sperm oxidative defenses but does not cause oxidative DNA damage in infertile men.OxiSelect™ Cellular UV- OXI TEK (Oxidative Stress DNA (cytosine 5) methyltr Anti CML Monoclonal Antib Anti CML Monoclonal Antib removed without changing 8 Isoprostane oxidative s Herring Sperm DNA Salmon Sperm DNA CAL-101 Mechanisms: PI3K- BYL-719 Mechanisms: PI3K- GSK-2636771 Mechanisms: P
#17555871 2007/07/09 Save this To Up
Relationship between DNA damage and total antioxidant capacity in patients with transitional meningioma.The purpose of this study was to assess oxidative DNA damage and total antioxidant capacity (TAC) in patients with transitional meningioma (TM) and to compare the results with normal brain tissues.
2649 related Products with: Relationship between DNA damage and total antioxidant capacity in patients with transitional meningioma.Total Antioxidant Capacit Total Antioxidant Capacit NWLSS™ TAC Peroxyl Assa OxiSelect™ Cellular UV- DNA (cytosine 5) methyltr Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri removed without changing Pfu DNA Polymerase (Not a Pfu DNA Polymerase (Not a Tfi DNA Ligase Includes w Tfi DNA Ligase Includes w
#17359903 2007/03/15 Save this To Up
Relationship between DNA damage and total antioxidant capacity in patients with glioblastoma multiforme.To assess oxidative DNA damage and total antioxidant capacity (TAC) in glioblastoma multiforme (GBM) and to compare the results with normal brain tissues.
1609 related Products with: Relationship between DNA damage and total antioxidant capacity in patients with glioblastoma multiforme.Total Antioxidant Capacit Total Antioxidant Capacit NWLSS™ TAC Peroxyl Assa OxiSelect™ Cellular UV- DNA (cytosine 5) methyltr Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri removed without changing Brain glioblastoma tissue Pfu DNA Polymerase (Not a Pfu DNA Polymerase (Not a Tfi DNA Ligase Includes w
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