Only in Titles

Search results for: Gold Chloride Solution (0.2%)

paperclip

#36748195   // To Up

Identifying Active Progeny Virus Particles in Formalin-Fixed, Paraffin-Embedded Sections Using Correlative Light and Scanning Electron Microscopy.

Immunohistochemical analysis of formalin-fixed paraffin-embedded (FFPE) tissue blocks is routinely used to identify virus-infected cells. However, detecting virus particles in FFPE sections using light microscopy is difficult because of the light diffraction resolution limitations of an optical microscope. In this study, light microscopy and field emission scanning electron microscopy were performed to observe 3-dimensional virus particles in FFPE sections in a nondestructive manner using NanoSuit or osmium conductive treatment methods. The virus particles in FFPE sections were immunostained with specific antibodies against the surface antigens of the viral particles and stained with 3,3'-diaminobenzidine. A metal solution (0.2% gold chloride or 2% osmium tetroxide) was applied to enhance the 3,3'-diaminobenzidine-stained area. This procedure is nondestructive for FFPE sections and is a simpler method than transmission electron microscopy. To validate the applicability of this technique, we performed 3-dimensional imaging of the virus particles of different sizes, such as human papillomavirus, cytomegalovirus, and varicella-zoster virus. Furthermore, ultrathin sections from the FFPE sections that were observed to harbor viral particles using field emission scanning electron microscopy were prepared and assessed using transmission electron microscopy. In the correlative areas, transmission electron microscopy confirmed the presence of large numbers of virus particles. These results indicated that the combination of marking viral particles with 3,3'-diaminobenzidine/metal staining and conductive treatment can identify active progeny virus particles in FFPE sections using scanning electron microscopy. This easy correlative imaging of field emission scanning electron microscopy of the identical area of FFPE in light microscopy may help elucidate new pathological mechanisms of virus-related diseases.
Toshiya Itoh, Satoshi Yamada, Isao Ohta, Shiori Meguro, Isao Kosugi, Toshihide Iwashita, Hiroaki Itoh, Naohiro Kanayama, Koji Okudela, Haruhiko Sugimura, Kiyoshi Misawa, Takahiko Hariyama, Hideya Kawasaki

1105 related Products with: Identifying Active Progeny Virus Particles in Formalin-Fixed, Paraffin-Embedded Sections Using Correlative Light and Scanning Electron Microscopy.

0.1ml (1mg/ml)50ug50ug0.1ml (1mg/ml)50 ug1 mL0.1 mg

Related Pathways

paperclip

#21601026   2011/04/01 To Up

Determination of antioxidant additives in foodstuffs by direct measurement of gold nanoparticle formation using resonance light scattering detection.

The capability of antioxidant compounds to reduce gold(III) to gold nanoparticles has been kinetically studied in the presence of cetyltrimethylammonium bromide using stopped-flow mixing technique and resonance light scattering as detection system. This study has given rise to a simple and rapid method for the determination of several synthetic and natural antioxidants used as additives in foodstuff samples. The formation of AuNPs was monitored by measuring the initial reaction-rate of the system in about 5s, using an integration time of 0.1s. Dynamic ranges of the calibration graphs and detection limits, obtained with standard solutions of the analytes, were (μmolL⁻¹): gallic acid (0.04-0.59, 0.01), propyl gallate (0.04-1.41, 0.01), octyl gallate (0.03-0.35, 0.08), dodecyl gallate (0.02-0.30, 0.007), butylated hydroxyanisol (0.07-0.39, 0.009), butylated hydroxytoluene (0.04-0.32, 0.01), ascorbic acid (0.11-1.72, 0.03) and sodium citrate (0.07-1.29, 0.02). The regression coefficients were higher than 0.994 in all instances. The precision of the method, expressed as RSD%, was established at two concentration levels of each analyte, with values ranging between 0.6 and 4.8%. The practical usefulness of the developed method was demonstrated by the determination of several antioxidant additives in foodstuff samples, which were extracted, appropriately diluted and assayed, obtaining recoveries between 95.4 and 99.5%. The results obtained were validated using two reference methods.
A Andreu-Navarro, J M Fernández-Romero, A Gómez-Hens

2347 related Products with: Determination of antioxidant additives in foodstuffs by direct measurement of gold nanoparticle formation using resonance light scattering detection.

100tests100ug Lyophilized100tests100ug Lyophilized400Tests48 samples16 Arrays/Slide100tests5 mg

Related Pathways

paperclip

#6153491   // To Up

A new staining method of astrocytes for paraffin section.

A new method of staining astrocytes in formation-fixed, paraffin-embedded sections was devised: (1) fix them in 5% mercuric chloride solution for 30 min to 1 h at 56 degrees C, (2) then place in 0.5% iodine alcohol for 5 min followed by placing in 0.5% sodium thiosulfate for 5 min, (3) immerse in 0.25% potassium permanganate for 3 min, (4) place in 2% oxalic acid for 2 min. (5) mordant in 2% iron alum for 45 s, and (6) place in 2% silver nitrate solution for 30 min. The next step is impregnation in ammoniacal silver solution for 10 -- 15 min at 56 degrees C, followed by reduction in neutral formalin and 2% iron alum, toning in 0.2% gold chloride, and fixing in 5% sodium thiosulfate. Pathological astrocytes of fibrillary and protoplasmic types were distincly demonstrated, although nerve cells and nuclei of oligodendrocytes and microglial cells were also faintly stained. Thus, the staining for paraffin sections is fairly selective for astrocytes in pathological states.
T Kitoh, M Matsushita

1414 related Products with: A new staining method of astrocytes for paraffin section.

0.1ml (1mg/ml)100Tests0.1 ml100Tests 70 Slides 100 assays10 mg100μg 500 Slides

Related Pathways