Search results for: Human Albumin ELISA Kit
#28889209 2017/09/10 Save this To Up
Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum.Immunoglobulin G (IgG) represents the major fraction of antibodies in healthy adult human serum, and deviations from physiological levels are a generic marker of disease corresponding to different pathologies. Therefore, screening methods for IgG evaluation are a valuable aid to diagnostics. The present work proposes a rapid, automatic, and miniaturized method based on UV-vis micro-bead injection spectroscopy (μ-BIS) for the real-time determination of human serum IgG with label-free detection. Relying on attachment of IgG in rec-protein G immobilized in Sepharose 4B, a bioaffinity column is automatically assembled, where IgG is selectively retained and determined by on-column optical density measurement. A "dilution-and-shoot" approach (50 to 200 times) was implemented without further sample treatment because interferences were flushed out of the column upon sample loading, with minimization of carryover and cross-contamination by automatically discarding the sorbent (0.2 mg) after each determination. No interference from human serum albumin at 60 mg mL(-1) in undiluted sample was found. The method allowed IgG determination in the range 100-300 μg mL(-1) (corresponding to 5.0-60 mg mL(-1) in undiluted samples), with a detection limit of 33 μg mL(-1) (1.7 mg mL(-1) for samples, dilution factor of 50). RSD values were < 9.4 and < 11.7%, for intra and inter-assay precision, respectively, while recovery values for human serum spiked with IgG at high pathological levels were 97.8-101.4%. Comparison to commercial ELISA kit showed no significant difference for tested samples (n = 8). Moreover, time-to-result decreased from several hours to < 5 min and analysis cost decreased 10 times, showing the potential of the proposed approach as a point-of-care method. Graphical abstract Micro-Bead Injection Spectroscopy method for real time, automated and label-free determination of total serum human Immunoglobulin G (IgG). The method was designed for Lab-on-Valve (LOV) platforms using a miniaturised protein G bioaffinity separative approach. IgG are separated from serum matrix components upon quantification with low non-specific binding in less than 5 min.
2406 related Products with: Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum.Sterile filtered goat se Sterile filtered goat se Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Growth Differentiation Fa Human Serum Albumin antib Human Insulin-like Growth Human Epstein-Barr Virus Human Gro g Macrophage In Human Insulin-like Growth Human Interferon-gamma IF Goat Anti-Human Synaptota
#28816622 2017/08/17 Save this To Up
Obtention and characterization of the recombinant simian Interleukin-15 in Escherichia coli for the preclinical assessment of an IL-15-based therapeutic vaccine.Recombinant simian IL-15 (siIL-15) was obtained for the preclinical assessment of an anti-human IL-15 vaccine. For this purpose, the cDNA from peripheral blood mononuclear cells of a Macaca fascicularis monkey was cloned into a pIL-2 vector. The siIL-15 was expressed in Escherichia coli strain W3110 as an insoluble protein which accounted for 13% of the total cellular proteins. Inclusion bodies were solubilized in an 8 M urea solution, which was purified by ion exchange and reverse phase chromatography up to 92% purity. The protein identity was validated by electrospray ionization-mass spectrometry, confirming the presence of the amino acids which distinguish the siIL-15 from human IL-15. The purified siIL-15 stimulates the proliferation of cytotoxic T-lymphocytes line (CTLL)-2 and Kit 225 cells with EC50 values of 3.1 and 32.5 ng/mL, respectively. Antisera from modified human IL-15-immunized macaques were reactive to human and simian IL-15 in enzyme-linked immunosorbent assays. Moreover, the anti-human IL-15 antibodies from immune sera inhibited siIL-15 activity in CTLL-2 and Kit 225 cells, supporting the activity and purity of recombinant siIL-15. These results indicate that the recombinant siIL-15 is biologically active in two IL-15-dependent cell lines, and it is also suitable for the preclinical evaluation of an IL-15-based therapeutic vaccine.
2240 related Products with: Obtention and characterization of the recombinant simian Interleukin-15 in Escherichia coli for the preclinical assessment of an IL-15-based therapeutic vaccine.Mouse Anti-Human Interleu Mouse Anti-Human Interleu Recombinant Human Interle Recombinant Human Interle Recombinant Porcine Inter Recombinant Porcine Inter Recombinant Rat Interleuk Recombinant Rat Interleuk FDA Standard Frozen Tissu Normal mouse multiple org Human Interleukin-15 IL-1 Mouse Interleukin-15 IL-1
#28257085 2017/03/03 Save this To Up
Vegetable and Fruit Intakes Are Associated with hs-CRP Levels in Pre-Pubertal Girls.The influence of diet on inflammation in children remains unclear. We aimed to analyze the influence of diet on high-sensitivity C-reactive protein (hs-CRP) levels in a pre-pubertal population free of other influences that may affect hs-CRP levels. We determined hs-CRP levels in 571 six- to eight-year-old children using an hs-CRP ELISA kit. Information on food and nutrient intake was obtained through a food-frequency questionnaire. Overall dietary quality was assessed using the Healthy Eating Index (HEI). We found that girls in the highest tertile of hs-CRP levels had a higher intake of saturated fatty acid, and lower intakes of fiber and vitamin E and a lower HEI score when compared to those in tertiles 1 and 2. We also observed a significant decrease in fruit and vegetable intakes by hs-CRP tertile. Factor analysis showed that a dietary pattern that was loaded most strongly with vegetable, fruit, fiber and vitamin A and E intakes correlated negatively (-0.132, p < 0.05) with hs-CRP. No such association was found in boys. In conclusion, our data show that girls with a poorer quality diet show higher hs-CRP levels already at a pre-pubertal age.
2984 related Products with: Vegetable and Fruit Intakes Are Associated with hs-CRP Levels in Pre-Pubertal Girls.Goat Anti-Human, Mouse AR Human high sensitivity in CCND3 & AREG Protein Prot Goat Anti-Human, Mouse, R Syringe pump can be contr DyNA Light UV Transillumi Biocidal ZF, spray disinf Biocidal ZF, spray disinf Biocidal ZF, spray disinf Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se
#27834063 2016/11/11 Save this To Up
Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status.The associations of vitamin D deficiency with various clinical conditions highlighted the importance of vitamin D testing. Currently, clinicians measure only the total 25-hydroxyvitamin D [25(OH)D] concentration, regardless of its bioavailability. We aimed to determine the effect of vitamin D-binding protein (VDBP) on 25(OH)D bioavailability.
2258 related Products with: Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status.Goat Anti-Human Vitamin D anti-Vitamin D binding pr Vitamin D3 (Cholecalcifer Vitamin D2 (Ergocalcifero Mouse Anti-1,25 Dihydroxy Mouse Anti-25 OH Vitamin E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran Taq SSB (Single Stranded Taq SSB (Single Stranded
#27230955 2016/05/27 Save this To Up
Investigation of the Levels of Serum Amyloid A, YKL-40, and Pentraxin-3 in Patients with Familial Mediterranean Fever.Familial Mediterranean Fever (FMF) is an autosomal recessive form of recurrent episodes of fever and an autoinflammatory disease characterized by inflammation of the serous membranes. The clinical diagnosis is supported by the laboratory findings. This study investigated the relationship of Serum Amyloid A (SAA), YKL-40, and Pentraxin-3 (PTX-3) with the FMF disease.
2990 related Products with: Investigation of the Levels of Serum Amyloid A, YKL-40, and Pentraxin-3 in Patients with Familial Mediterranean Fever.Sterile filtered mouse s Beta Amyloid (1 40) ELISA Beta Amyloid (40) ELISA K Beta Amyloid (1 40) ELISA Lung adenocarcinoma and n Lung adenocarcinoma tissu Mouse Anti-beta-Amyloid(1 FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu
#27132791 2016/05/02 Save this To Up
Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus.This study was designed to determine the diagnostic role of urinary kidney injury molecule (KIM)-1 levels in renal damage in patients with type 2 diabetes mellitus according to the urinary albumin/creatinine ratio.
Human Kidney injury molec Kidney clear cell carcino High density (188 cases 2 High density (188 cases 2 Kidney cancer tissue arra Kidney cancer survey tiss Kidney cancer survey tiss Kidney cancer survey tiss High density kidney cance Kidney cancer tissue arra Kidney cancer survey tiss Kidney cancer survey tiss
#27513835 2016/08/12 Save this To Up
Urine NGAL is useful in the clinical evaluation of renal function in the early course of acute pancreatitis.Acute Kidney Injury (AKI) is a serious early complications in patients with acute pancreatitis (AP) that signifcantly increases mortality rates compared to patients without AKI. The early diagnosis of AKI during its treatable phases and implementation of appropriate treatment protocols can improve outcomes for this group of patients. A promising biomarker for AKI is neutrophil gelatinase-associated lipocalin (NGAL).
1171 related Products with: Urine NGAL is useful in the clinical evaluation of renal function in the early course of acute pancreatitis.Multiple organ tumor tiss Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Kidney cancer test tissue MultiGene Gradient therm Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti
#27372570 2016/07/03 Save this To Up
IL-33 promotes food anaphylaxis in epicutaneously sensitized mice by targeting mast cells.Cutaneous exposure to food allergens predisposes to food allergy, which is commonly associated with atopic dermatitis (AD). Levels of the epithelial cytokine IL-33 are increased in skin lesions and serum of patients with AD. Mast cells (MCs) play a critical role in food-induced anaphylaxis and express the IL-33 receptor ST2. The role of IL-33 in patients with MC-dependent food anaphylaxis is unknown.
2120 related Products with: IL-33 promotes food anaphylaxis in epicutaneously sensitized mice by targeting mast cells.Human Interleukin-33 IL-3 Recombinant Mouse Interle GLP 2 ELISA Kit, Rat Prog Anti C Reactive Protein A anti HSV (II) gB IgG1 (mo anti HCMV IE pp65 IgG1 (m anti HCMV gB IgG1 (monocl CELLKINES Natural Human I Human Interleukin-4 IL-4 Human Interleukin-6 IL-6 Human Interleukin-7 IL-7 Human Interleukin-2 IL-2
#27366878 2016/09/14 Save this To Up
Development of a high sensitivity C-reactive protein immunoassay and comparison with a commercial kit.C-reactive protein (CRP) is intricately sensitive marker of inflammation, infection, and tissue damage. Role in the prognosis of heart diseases has been recently discovered. This study aimed to develop a cost-effective and high-sensitivity CRP immunoassay for use in cardiac risk assessment. Assay was optimized for coating, blocking of capturing antibody, dilution, and reaction time of the conjugate and sample volume. For normal reference range, CRP was determined in serum samples from apparently healthy volunteers. For clinical validation, CRP was determined in samples of acute coronary syndrome patients by in-house and commercial assays. The lower detection limit of in-house assay was 0.16 µg/L. Intra and inter assay imprecision was 4.39%, 4.6% and 8.6%, 9.3%, respectively. The correlation between the CRP levels by the two assays was r = 0.861. Sensitivity, specificity, predictive value for a positive test, and a negative test of in-house assay was 95.3%, 92.8%, 95.3%, and 92.8%, respectively. At lower-end CRP levels of both kits correlated very well but showed variation at upper end. In-house assay showed high sensitivity and reliability at lower end and it is hoped that will help to evaluate cardiac risk assessment (after improvement at upper end) in clinically poor settings.
2053 related Products with: Development of a high sensitivity C-reactive protein immunoassay and comparison with a commercial kit.Beta Amyloid (1 42) High Beta Amyloid (1 42) High MarkerGeneTM TAMRA Antibo MarkerGene™ Cellular Se ELISA Human , C-Reactive ELISA Canine , C-Reactive ELISA Rat , C-Reactive Pr ELISA Mouse , C-Reactive Anti C-Reactive Protein A Anti C Reactive Protein Anti C Reactive Protein A ReadiLink™ mFluor™ Vi
#27266918 2016/07/26 Save this To Up
Serum apelin is associated with affective disorders in peritoneal dialysis patients.Depression and anxiety are prevalent affective disorders in peritoneal dialysis (PD) patients. Recent research has proposed a potential role of apelinergic system in pathogenesis of depression. The present study aimed to evaluate the frequency of depression and anxiety and their potential relation with serum apelin levels among PD patients.
2121 related Products with: Serum apelin is associated with affective disorders in peritoneal dialysis patients.Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon HIV1 integrase antibody, Human Serum Albumin antib
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