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           Search results for: Human Chorionic Gonadotropin (hCG) ELISA Kit   

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#26968108   2016/05/02 Save this To Up

Serum activin B concentration as predictive biomarker for ectopic pregnancy.

We evaluated the diagnostic accuracy of activin B in discriminating tubal ectopic pregnancy (tEP) from intrauterine miscarriages (IUM), and normal viable intrauterine pregnancy (IUP). We included 28 women with tEP, 31 women with IUM, and 29 normal IUP, confirmed both by clinical examination and ultrasonography. Serum activin B concentration was measured at the time of admission using the ELISA kit. The median serum activin B concentration was found to be significantly decreased in both tEP (p=0.004) and IUM (p=0.022) compared to normal IUP. When compared between tEP and IUM, activin B concentrations did not differ significantly. ROC analysis of activin B and free β-hCG demonstrated AUC of 0.722 and 0.805, respectively to discriminate tEP from viable IUP. The model including both activin B and free β-hCG improved the discriminating potential with greater AUC (0.824), and specificity (93%) than individual one. To discriminate tEP from IUM, activin B, free β-hCG and combination of both performed poorly. We conclude that serum activin B concentration is lower in tubal ectopic pregnancy, and can discriminate it from normal pregnancy with moderate accuracy. It also shows improved diagnostic potential along with free β-hCG, but cannot distinguish tEP from IUM reliably.

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#25616588   2015/06/06 Save this To Up

Transient false-positive urine human chorionic gonadotropin in septic shock.

Point-of-care testing for urine human chorionic gonadotropin (hCG) allows rapid diagnosis of pregnancy and pregnancy-related disorders at the bedside. Urine hCG test kits use enzyme-linked immunosorbent assay technology and incorporate 2 types of monoclonal antibody in a sandwich structure. There have been case reports in a variety of disease states reporting interference with this method leading to false-positive results. We describe the case of a nonpregnant female presenting to the emergency department with septic shock secondary to severe colitis. Three sequential urine tests using the Clearview hCG test kit (Alere Limited, Stockport, United Kingdom) yielded positive results, whereas quantitative serum analysis was negative for hCG. This initial test result reverted to a true-negative result after 48 hours, suggesting the transient passage of an interferent into the urine at the time of initial testing. This may have been a molecule produced as part of the host inflammatory response or from bacterial synthesis of an interferent with hCG-like antigenic structure. It is important that clinicians are aware of the mechanisms and limitations of urine hCG testing and maintain a low threshold to undertake early serum hCG testing to confirm diagnosis.

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#25057080   2014/07/24 Save this To Up

[Association of human chorionic gonadotropin level in embryo culture media with early embryo development].

To investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development.

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#19142010   2009/04/17 Save this To Up

Human chorionic gonadotropin in cervicovaginal secretions as a predictor of preterm birth.

To compare the qualitative (QL) and quantitative (QN) levels of betahCG in cervicovaginal secretions as predictors of preterm birth.

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#18412774   2008/04/16 Save this To Up

Stimuli through Toll-like receptor (TLR) 3 and 9 affect human chorionic gonadotropin (hCG) production in a choriocarcinoma cell line.

Toll-like receptors (TLR) are expressed mostly on mucosal innate immune cells which recognize microorganism-associated structures, the so called pathogen-associated molecular patterns (PAMP). Recently, possible roles of TLR in placental tissue, especially their role in protecting the conceptus from maternal infection, have been discussed, but the physiopathological roles of TLR in placental functions have yet to be examined.

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#16643807   2006/04/28 Save this To Up

[Methodological study on magnetic enzymic immunoassay for detecting free hCGbeta subunit].

To establish a novel magnetic enzyme immunoassay (MEIA) for detecting human choriogonadotropin free beta subunit (hCGbeta).

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#15043467   2004/03/26 Save this To Up

Evaluation of a dimeric inhibin-A assay for assessing fetal Down syndrome: establishment, comparison, and monitoring of median concentrations for normal pregnancies.

Several studies report the role of dimeric inhibin-A in assessing risk for fetal Down syndrome. The majority, however, use the Serotec inhibin-A assay and not the newer Diagnostic Systems Laboratories inhibin-A enzyme-linked immunosorbent assay (ELISA).

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#12768561   2003/05/27 Save this To Up

Three-dimensional power Doppler imaging of ovarian stromal blood flow in women with endometriosis undergoing in vitro fertilization.

The aims of this retrospective study were to investigate whether the quantification of ovarian stromal blood flow and/or leptin concentration are predictive of in vitro fertilization (IVF) outcomes in women after laparoscopic ovarian cystectomy for large endometriomas.

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#12134909   2002/07/23 Save this To Up

Diagnostic significance of soluble c-kit in the cerebrospinal fluid of patients with germ cell tumors.

Overexpression of the protooncogene c-kit has been suggested in a gonadal germ cell tumor (GCT). Recently, the soluble isoform of c-kit (s-kit) has been expressed in a variety of cell types. The goal of this study was to investigate the expression of c-kit and the clinical significance of s-kit in patients with GCTs.

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#11728769   2001/11/30 Save this To Up

False-positive urine beta-HCG in a woman with a tubo-ovarian abscess.

Modern urine beta-human chorionic gonadotropin (HCG) assays that use enzyme-linked immunosorbent assay (ELISA) technology are sensitive and specific for diagnosing pregnancy, both intrauterine and ectopic, and have become indispensable to the practice of Emergency Medicine. A urine HCG test is often relied on by the Emergency Physician as a critical component in the diagnostic regimen of a patient with a possible ectopic pregnancy. We report a case of a false-positive urine beta-HCG test in a patient with a ruptured tubo-ovarian abscess. Though false-positive pregnancy tests with tubo-ovarian abscesses have previously been reported with older methods of HCG detection, we believe that this is the first case where the pregnancy test was the modern ELISA type. The mechanism for the false-positive reaction in this case is unknown, but time may show that the ELISA test kit, like its predecessors, may occasionally give a false-positive reaction in this class of patients.

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