Search results for: Human Cord Blood CD34+ Cells Derived Endothelial Cells
#31178416 // Save this To Up
Overexpression of GATA2 Enhances Development and Maintenance of Human Embryonic Stem Cell-Derived Hematopoietic Stem Cell-like Progenitors.GATA2 is essential for the endothelial-to-hematopoietic transition (EHT) and generation of hematopoietic stem cells (HSCs). It is poorly understood how GATA2 controls the development of human pluripotent stem cell (hPSC)-derived HS-like cells. Here, using human embryonic stem cells (hESCs) in which GATA2 overexpression was induced by doxycycline (Dox), we elucidated the dual functions of GATA2 in definitive hematopoiesis before and after the emergence of CD34CD45CD90CD38 HS-like cells. Specifically, GATA2 promoted expansion of hemogenic precursors via the EHT and then helped to maintain HS-like cells in a quiescent state by regulating cell cycle. RNA sequencing showed that hPSC-derived HS-like cells were very similar to human fetal liver-derived HSCs. Our findings will help to elucidate the mechanism that controls the early stages of human definitive hematopoiesis and may help to develop a strategy to generate hPSC-derived HSCs.
1138 related Products with: Overexpression of GATA2 Enhances Development and Maintenance of Human Embryonic Stem Cell-Derived Hematopoietic Stem Cell-like Progenitors.Stem Cell Products TGF-b1 Human Stem Cell Factor SC Stem Cell Products TGF-b1 Stem Cell Products TGF-b1 ELISA Human , Stem Cell F Mesenchymal Stem Cell Adi Stem cell miRNA Array DiscoveryPak™ Stem Cell DiscoveryPak™ Stem Cell Rat Mesenchymal Stem Cell Mouse Stem Cell Factor SC DiscoveryPak™ Stem Cell
#31140023 // Save this To Up
Effect of intracameral human cord blood-derived stem cells on lasered rabbit trabecular meshwork.This study aimed to investigate the effect of intracameral human cord blood stem cells on lasered rabbit trabecular meshwork.
1169 related Products with: Effect of intracameral human cord blood-derived stem cells on lasered rabbit trabecular meshwork.Human Cord Blood CD34+ Ce Rabbit Anti-Human Red Blo AccuPrep Genomic DNA Extr Human Red Blood Cells Uni Human Cord Blood 1ml Rabbit Red Blood Cells 10 LumiSTEM 96 iPS MSC deriv Blood Group Lewis a antib thymic dendritic cell-der Macrophage Colony Stimula Astra Blue 6GLL, Stain fo Rabbit Red Blood Cells 30
#30918315 // Save this To Up
Human Bone Marrow Endothelial Progenitor Cell Transplantation into Symptomatic ALS Mice Delays Disease Progression and Increases Motor Neuron Survival by Repairing Blood-Spinal Cord Barrier.Convincing evidence demonstrated impairment of the blood-spinal cord barrier (BSCB) in Amyotrophic Lateral Sclerosis (ALS), mainly by endothelial cell (EC) alterations. Replacing damaged ECs by cell transplantation is a potential barrier repair strategy. Recently, we showed that intravenous (iv) administration of human bone marrow CD34 (hBM34) cells into symptomatic ALS mice benefits BSCB restoration and postpones disease progression. However, delayed effect on motor function and some severely damaged capillaries were noted. We hypothesized that hematopoietic cells from a restricted lineage would be more effective. This study aimed to establish the effects of human bone marrow-derived endothelial progenitor cells (hBMEPCs) systemically transplanted into G93A mice at symptomatic disease stage. Results showed that transplanted hBMEPCs significantly improved behavioral disease outcomes, engrafted widely into capillaries of the gray/white matter spinal cord and brain motor cortex/brainstem, substantially restored capillary ultrastructure, significantly decreased EB extravasation into spinal cord parenchyma, meaningfully re-established perivascular astrocyte end-feet, and enhanced spinal cord motor neuron survival. These results provide novel evidence that transplantation of hBMEPCs effectively repairs the BSCB, potentially preventing entry of detrimental peripheral factors, including immune/inflammatory cells, which contribute to motor neuron dysfunction. Transplanting EC progenitor cells may be a promising strategy for barrier repair therapy in this disease.
1867 related Products with: Human Bone Marrow Endothelial Progenitor Cell Transplantation into Symptomatic ALS Mice Delays Disease Progression and Increases Motor Neuron Survival by Repairing Blood-Spinal Cord Barrier.Human Cord Blood CD34+ Ce Human Internal Mammary Ar Human Uterine Microvascul Monoclonal Mouse Anti Hum Human Umbilical Vein Endo GFP Expressing Human Derm GFP Expressing Human Coro Human Liver Sinusoidal Mi Anti C Reactive Protein A Human Iliac Artery Endoth Human Brain Microvascular Human Aortic Artery Endot
#30416049 // Save this To Up
Patient-Specific iPSC-Derived Endothelial Cells Provide Long-Term Phenotypic Correction of Hemophilia A.We generated patient-specific disease-free induced pluripotent stem cells (iPSCs) from peripheral blood CD34+ cells and differentiated them into functional endothelial cells (ECs) secreting factor VIII (FVIII) for gene and cell therapy approaches to cure hemophilia A (HA), an X-linked bleeding disorder caused by F8 mutations. iPSCs were transduced with a lentiviral vector carrying FVIII transgene driven by an endothelial-specific promoter (VEC) and differentiated into bona fide ECs using an optimized protocol. FVIII-expressing ECs were intraportally transplanted in monocrotaline-conditioned non-obese diabetic (NOD) severe combined immune-deficient (scid)-IL2rγ null HA mice generating a chimeric liver with functional human ECs. Transplanted cells engrafted and proliferated in the liver along sinusoids, in the long term showed stable therapeutic FVIII activity (6%). These results demonstrate that the hemophilic phenotype can be rescued by transplantation of ECs derived from HA FVIII-corrected iPSCs, confirming the feasibility of cell-reprogramming strategy in patient-derived cells as an approach for HA gene and cell therapy.
2733 related Products with: Patient-Specific iPSC-Derived Endothelial Cells Provide Long-Term Phenotypic Correction of Hemophilia A.Human Cord Blood CD34+ Ce 1,1'-Dioctadecyl-3,3,3',3 GFP Expressing Human Inte Mouse Anti-Pig Endothelia Human Dermal Lymphatic Mi Human Coronary Artery End GFP Expressing Human Live Human Cardiac Microvascul GFP Expressing Mouse Brai RFP Expressing Human Umbi LumiSTEM 96 iPS MSC deriv Human Dermal Microvascula
#29908162 // Save this To Up
Isolation and characterization of endothelial colony-forming cells from mononuclear cells of rat bone marrow.Transplantation of bone marrow-derived endothelial progenitor cells (BM-EPCs) has been used as a therapeutic strategy for vascular repair. However, it remains controversial whether BM-EPCs exhibit clonal endothelial colony-forming cell (ECFC) capacity, a characteristic of true EPCs. The aim of this study was to isolate and explore the cellular properties of BM-ECFCs. We isolated BM-ECFCs from rat bone marrow with high purity via an optimized method. This approach involved the removal of selective colonies based on the conventional differential adhesive culture method used to isolate ECFCs from peripheral and umbilical cord blood. Our results indicate that primary colony BM-ECFCs display a panel of surface antigen markers consistent with endothelial cells. These BM-ECFCs coexpress CD34, CD133, and VEGFR2 at high levels, and these levels decrease with passaging. These cells have high potential for proliferation, migration, and formation of capillary-like structures on Matrigel, and these abilities are retained during ex vivo expansion. Furthermore, BM-ECFCs cultured with 10% or 20% fetal bovine serum demonstrated two different patterns of spontaneous capillary-like structure formation. These results provide a foundation for isolation of ECFCs from human bone marrow for autologous cell transplantation and tissue engineering applications in the future.
2919 related Products with: Isolation and characterization of endothelial colony-forming cells from mononuclear cells of rat bone marrow.NycoPrep™ 1.077, for is Human Tonsil Microvascula GFP Expressing Human Saph GFP Expressing Human Umbi Human Glomerular Microvas Human Pancreatic Microvas Human Liver Sinusoidal Mi Human Internal Mammary Ar Human Uterine Microvascul GFP Expressing Human Brai GFP Expressing Human Aort GFP Expressing Human Reti
#29895317 // Save this To Up
Hypoxia with Wharton's jelly mesenchymal stem cell coculture maintains stemness of umbilical cord blood-derived CD34 cells.The physiological approach suggests that an environment associating mesenchymal stromal cells with low O concentration would be most favorable for the maintenance of hematopoietic stem/progenitor cells (HSPCs). To test this hypothesis, we performed a coculture of cord blood CD34 cells with Wharton's jelly mesenchymal stem cells (WJ-MSCs) under different O concentration to simulate the growth of HSPCs in vivo, and assessed the impacts on stemness maintenance and proliferation of cord blood HSPCs in vitro.
2485 related Products with: Hypoxia with Wharton's jelly mesenchymal stem cell coculture maintains stemness of umbilical cord blood-derived CD34 cells.Human Cord Blood CD34+ Ce Rat Mesenchymal Stem Cell Mouse Anti-Human CD34 Tar Sheep Red Blood Cells, Pa Sheep Red Blood Cells, Pa Macrophage Colony Stimula Mesenchymal Stem Cell Adi Astra Blue 6GLL, Stain fo Stemez hN2 Human Neuron D AccuzolTM Total RNA Extra Sheep Red Blood Cells, Pa Sheep Red Blood Cells, Pa
#29732993 // Save this To Up
Endothelial Progenitor Cells as Molecular Targets in Vascular Senescence and Repair.Endothelial progenitor cells are circulating blood cells derived from various sources like bone marrow, spleen, umbilical cord, liver, kidney and other sources that play a vital role in the regeneration of the endothelial lining of blood vessels and wound repair. There are two types of EPCs, early EPCs and late EPCs. EPCs are believed to originate from hematopoietic stem cells and mesenchymal stem cells. The mobilization of progenitor cells from bone marrow to the peripheral circulation is highly regulated under both normal physiological conditions and stress. EPCs contribute to neovascularization and tissue repair in the musculoskeletal, neural tissues and the bone which are mobilized and recruited to the injured tissue. Cell-based therapies of endothelial progenitor cells are time-consuming and expensive for performing in-vitro cell expansion procedures. New therapeutic approaches are being developed using animal models based on the specific functions of EPC in and experiments which have revealed the importance of various signalling pathways. It has been clear that the activation state of EPCs is critical to the vessel repair process and the role has not been completely understood.
2504 related Products with: Endothelial Progenitor Cells as Molecular Targets in Vascular Senescence and Repair.GLP 1 ELISA Kit, Rat Gluc MarkerGene™ Cellular Se Human Large Intestine Mic GFP Expressing Human Inte Mouse Anti-Human Endothel MarkerGeneTM Live Dead As Human Small Intestine Mic Human Internal Mammary Ar Cultrex In Vitro Angiogen MarkerGene™ Cellular Se Human Brain Microvascular Amplite™ Fluorimetric P
#29720269 // Save this To Up
Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo.We have previously demonstrated that acidic preconditioning of human endothelial colony-forming cells (ECFC) increased proliferation, migration, and tubulogenesis in vitro, and increased their regenerative potential in a murine model of hind limb ischemia without baseline disease. We now analyze whether this strategy is also effective under adverse conditions for vasculogenesis, such as the presence of ischemia-related toxic molecules or diabetes, one of the main target diseases for cell therapy due to their well-known healing impairments.
2133 related Products with: Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo.Cultrex In Vitro Angiogen Human Internal Mammary Ar Macrophage Colony Stimula Macrophage Colony Stimula MarkerGeneTM in vivo lacZ Human Large Intestine Mic Insulin Glucose Phospho-S GLP 1 ELISA Kit, Rat Gluc GFP Expressing Human Inte Human Small Intestine Mic T-Cell Receptor Signaling Cytokine (Human) Antibody
#29659011 // Save this To Up
Immune system augmentation via humanization using stem/progenitor cells and bioengineering in a breast cancer model study.Despite significant advances, most current in vivo models fail to fully recapitulate the biological processes that occur in humans. Here we aimed to develop an advanced humanized model with features of an organ bone by providing different bone tissue cellular compartments including preosteoblasts, mesenchymal stem/stromal (MSCs), endothelial and hematopoietic cells in an engineered microenvironment. The bone compartment was generated by culturing the human MSCs, umbilical vein endothelial cells with gelatin methacryloyl hydrogels in the center of a melt-electrospun polycaprolactone tubular scaffolds, which were seeded with human preosteoblasts. The tissue engineered bone (TEB) was subcutaneously implanted into the NSG mice and formed a morphologically and functionally organ bone. Mice were further humanized through the tail vein injection of human cord blood derived CD34+ cells, which then populated in the mouse bone marrow, spleen and humanized TEB (hTEB). 11 weeks after CD34+ transplantation, metastatic breast cancer cells (MDA-MB-231BO) were orthotopically injected. Cancer cell injection resulted in the formation of a primary tumor and metastasis to the hTEB and mouse organs. Less frequent metastasis and lower tumor burden were observed in hematochimeric mice, suggesting an immune-mediated response against the breast cancer cells. Overall, our results demonstrate the efficacy of tissue engineering approaches to study species-specific cancer-bone interactions. Further studies using genetically modified hematopoietic stem cells and bioengineered microenvironments will enable us to address the specific roles of signaling molecules regulating hematopoietic niches and cancer metastasis in vivo.
2486 related Products with: Immune system augmentation via humanization using stem/progenitor cells and bioengineering in a breast cancer model study.Breast cancer tissue arra Breast cancer tissue arra Male genitourinary system Breast cancer test tissue Breast cancer test tissue Breast cancer tissue arra Breast cancer tissue arra Middle advanced stage bre Breast cancer tissue arra Breast cancer test tissue Breast cancer and normal Breast cancer tissue arra
#29473177 // Save this To Up
Manufacture of endothelial colony-forming progenitor cells from steady-state peripheral blood leukapheresis using pooled human platelet lysate.Endothelial colony-forming progenitor cells (ECFCs) are promising candidates for cell therapies. However, ECFC translation to the clinic requires optimized isolation and manufacture technologies according to good manufacturing practice (GMP).
2333 related Products with: Manufacture of endothelial colony-forming progenitor cells from steady-state peripheral blood leukapheresis using pooled human platelet lysate.Human Cord Blood CD34+ Ce Macrophage Colony Stimula Human Internal Mammary Ar Human Uterine Microvascul Human Umbilical Vein Endo GFP Expressing Human Derm GFP Expressing Human Coro Human Liver Sinusoidal Mi Astra Blue 6GLL, Stain fo Human Ovarian Microvascul Human Iliac Artery Endoth Human Brain Microvascular
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia