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#28712395   2017/07/17 Save this To Up

[Inhibitory effect of bispecific antibody targeting IL-12 p40 and TNF-α simultaneously on psoriasis in mice].

Objective To construct bispecific antibodies, which can block interleukin 12 (IL-12)/IL-23 p40 subunit and tumor necrosis factor α (TNF-α) simultaneously, and identify their biological function and inhibitory effect on psoriasis formation in mice. Methods Based on the sequences of adalimumab and ustekinumab, three kinds of bispecific antibodies were designed, named BiAU003, BiAU022 and BiAU023. The specificity and binding capacity of bispecific antibodies were determined by ELISA. After co-treated with bispecific antibodies and TNF-α, the level of endothelial leukocyte adhesion molecule-1 (ELMA-1) labeled with fluorescein isothiocyanate (FITC) in human umbilical vein endothelial cells (HUVECs) were examined by flow cytometry. Human peripheral blood mononuclear cells (PBMCs) were purified and cultured in the medium containing IL-2 and IL-12 in the absence or presence of bispecific antibodies. Commercial ELISA kit was used to detect interferon γ (IFN-γ) concentration in the supernatant. BALB/c mice were used for psoriasis model construction through injection of IL-12 and TNF-α subcutaneously. Then they were treated with the bispecific antibodies. Psoriasic skin was measured in thickness and scale under microscopy after H&E staining. Results The three kinds of bispecific antibodies could specifically recognize IL-12/23 p40 and TNF-α protein, and inhibit IFN-γ secretion and the expression of ELAM-1 protein. Data also indicated that bispecific antibodies inhibited the formation of psoriasic skin, and showed an equal or superior effect to control antibody drug. Conclusion The novel bispecific antibodies, BiAU003, BiAU022 and BiAU023, can serve as an antagonist of TNF-α and IL-12/23 p40, and have a blocking effect on mouse psoriasis formation.

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Rat Anti-Mouse Interleuki Rat Anti-Mouse Interleuki Recombinant Human Interle Recombinant Human Interle Interleukins Recombinant Interleukins Recombinant Rat Anti-Mouse Interleuki Rat Anti-Mouse Interleuki LPAM-1(Integrin α4, CD49 IL-12 Receptor beta2 anti IL-12Rbeta1 antibody Sour Recombinant Rat Interleuk

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#27778032   2016/10/25 Save this To Up

Activation of necroptosis in a rat model of acute respiratory distress syndrome induced by oleic acid.

The present study was aimed to investigate the role of necroptosis in the pathogenesis of acute respiratory distress syndrome (ARDS). The rat model of ARDS was induced by intravenous injection of oleic acid (OA), and observed for 4 h. The lung injury was evaluated by arterial blood gas, lung wet-dry weight ratio (W/D) and histological analyses. Simultaneously, bronchoalveolar lavage fluid (BALF) was collected for total and differential cell analysis and total protein determination. Tumor necrosis factor alpha (TNF-α) level in BALF was determined with a rat TNF-α ELISA kit. Expressions of receptor interacting protein kinase 1 (RIPK1), RIPK3 and mixed lineage kinase domain-like protein (MLKL) in lung tissue were determined by Western blot and immunohistochemical staining. The interaction between RIPK1 and RIPK3 was explored by immunoprecipitation. The results showed that, compared with those in control group, total white blood cells count (WBC), polymorphonuclear percentage (PMN%), total protein concentration, TNF-α level in BALF, W/D, and the alveolar-arterial oxygen tension difference (P(A-a)O2) in OA group were significantly increased at 4 h after OA injection. Western blot and immunostaining further showed remarkably increased expressions of RIPK1, RIPK3 and MLKL in lung tissue from OA group. Additionally, immunoprecipitation results indicated an enforced interaction between RIPK1 and RIPK3 in OA group. Collectively, the TNF-α level in BALF and the RIPK1-RIPK3-MLKL signaling pathway in lung tissue were found to be upregulated and activated with the process of ARDS. These findings implicate that RIPK1/RIPK3-mediated necroptosis plays a possible role in the pathogenesis of ARDS, which may provide a new idea to develop novel drugs for the therapy of ARDS.

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Rat intestinal fatty acid Anti-AICDA(Activation-ind Anti AICDA(Activation ind Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Goat Anti-Rat MARCH10, (i Goat Anti-Mouse, Rat DLL1 Goat Anti-Human, Mouse, R Goat Anti-Human, Mouse, R Goat Anti-Rat Connexin 43

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#26281248   2015/08/17 Save this To Up

[Experimental study on the effects of the nerve growth factor regulating calcitonin gene-related peptide in promoting the proliferation of MG-63 in vitro].

To investigate the nerve growth factor (NGF) regulating the expression of calcitonin gene-related peptide (CGRP) in promoting the proliferation of osteoblast-like cell (MG-63) and thus illustrate the mechanism of the NGF in wound healing.

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Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss MultiGene Gradient therm Human Insulin-like Growth Human Insulin-like Growth Macrophage Colony Stimula

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#25952949   2015/07/09 Save this To Up

Anti-inflammatory effects of hinokitiol on human corneal epithelial cells: an in vitro study.

This study assessed the anti-inflammatory effect and mechanism of action of hinokitiol in human corneal epithelial (HCE) cells.

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#25652862   2015/02/05 Save this To Up

[Leptin induces human lung fibroblast to trans-differentiate into myofibroblast].

To explore the effect of leptin on the transdifferentiation of human lung fibroblast to myofibroblast and its mechanism.

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Fibroblast Growth Factor Fibroblast Growth Factor Fibroblast Growth Factor Fibroblast Growth Factor Human Fibroblast Growth F Human Fibroblast Growth F Human Fibroblast Growth F Human Fibroblast Growth F Human Fibroblast Growth F Human Leptin Leptin Human Fibroblast Growth F Mouse Anti-Human Leptin

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#22152775   2011/12/14 Save this To Up

[Medicated serum prepared with Chinese herbal medicine Zhizhen Recipe down-regulates activity of nuclear factor-κB and expression of P-glycoprotein in human colorectal cancer multidrug-resistant cell line HCT-8/VCR].

To investigate the effects of medicated serum prepared with Chinese herbal medicine Zhizhen Recipe (ZZR) on activity of nuclear factor-κB (NF-κB) and expression and function of P-glycoprotein (P-gp) in human colorectal cancer multidrug-resistant cell line HCT-8/VCR.

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#21968312   2011/10/04 Save this To Up

[Monoclonal antibody preparation and identification of vascular endothelial growth factor 165 expressed in vitro].

To prepare a monoclonal antibody against human vascular endothelial growth factor (VEGF165), for further study the VEGF165 in the tumorigenesis, tumor cell migration and the tumor cells escape from the immune response.

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Human Vascular Endothelia Mouse Vascular Endothelia Rat Vascular Endothelial Human Endocrine Gland Vas Human Vascular Endothelia Mouse Vascular Endothelia Growth Factor (Human) Ant Mouse Vascular Endothelia Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse

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#21557913   2011/05/11 Save this To Up

[Preparation and identification of monoclonal antibody against human growth differentiation factor 15].

To prepare the monoclonal antibody (mAb) against human growth differentiation factor 15 (GDF15).

2825 related Products with: [Preparation and identification of monoclonal antibody against human growth differentiation factor 15].

Human Growth and Differen Human Growth and Differen Epidermal Growth Factor ( Epidermal Growth Factor ( Fibroblast Growth Factor Fibroblast Growth Factor Growth Differentiation Fa Growth Differentiation Fa Human Growth Hormone anti Human Growth Hormone anti Human Growth Hormone anti Human Growth and Differen

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#20937235   2010/10/12 Save this To Up

[Regulation on phenotypic and functional maturation of dendritic cells by apolipoprotein A-I].

to investigate the effects of apolipoprotein A-I (ApoA-I) on the peripherial blood dendritic cell (PBDC) and monocyte derived DC (MDDC) in vitro.

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Epidermal Growth Factor ( Epidermal Growth Factor ( GLP 2 ELISA Kit, Rat Prog Mouse Anti-Human Dendriti Rat Anti-Mouse Dendritic Mouse Anti-Human Follicul Mouse Anti-Human Follicul Mouse Anti-Human Follicul Mouse Anti-Human Follicul Apolipoprotein B (Apo B) Bcl-2 Oncoprotein; Clone Bcl-2 Oncoprotein; Clone

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#20619106   2010/07/12 Save this To Up

[Production and identification of a monoclonal antibody against human HPPCn].

To make monoclonal antibody(mAb) against human HPPCn for the use in research on HPPCn's function and its relationship with liver diseases.

2811 related Products with: [Production and identification of a monoclonal antibody against human HPPCn].

Anti C Reactive Protein A Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti Human AGO3, Monoclon anti CD16 monoclonal anti anti CD20 monoclonal anti anti CD54 IgG2b k monoclo anti CD66e IgG1 monoclona

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