Search results for: Human Hepatitis B Surface Antigen, HBsAg ELISA Kit
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Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products.In cell-based therapy, the transmission of communicable diseases imposes a substantial threat to recipients. In this study, we investigated whether cell-based screening could detect hepatitis B virus (HBV) in human umbilical cord-derived mesenchymal stem cells (HUMSCs) isolated from HBV-infected donors to understand the susceptibility of HUMSCs to HBV infection.
2348 related Products with: Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products.FDA Standard Frozen Tissu Oral squamous cell cancer Cell Meter™ Fluorimetri FDA Standard Frozen Tissu FDA Standard Frozen Tissu Cell Meter™ Fluorimetri Nycodenz, non ionic, non FDA Standard Frozen Tissu Human Mouse Rat JNK (T183 Human Mouse Rat Phospho-T Non small cell lung carci Human Mouse Rat Phospho-E
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Fluorescence ELISA based on glucose oxidase-mediated fluorescence quenching of quantum dots for highly sensitive detection of Hepatitis B.Herein, we present a novel sandwich fluorescence enzyme linked immunosorbent assay (ELISA) for highly sensitive detection of Hepatitis B virus surface antigen (HBsAg) based on glucose oxidase (GOx)-induced fluorescence quenching of mercaptopropionic acid-modified CdTe quantum dots (MPA-QDs). In this system, hydrogen peroxide (HO) sensitive MPA-QDs was used as a signal output, and glucose oxidase (GOx) was used as label which can generate HO via catalytic oxidation of glucose. The proposed method showed dynamic linear detection of HBsAg both in the range of 47pgmL ~ 380pgmL and 0.75ngmL ~ 12.12ngmL. The detection limit of the proposed fluorescence ELISA was 1.16pgmL, which was approximately 430-fold lower than that of horseradish peroxidase (HRP)-based conventional ELISA. The average recoveries for HBsAg-spiked serum samples ranged from 98.0% to 126.8% with the relative standard derivation below 10%, thus indicating acceptable precision and high reproducibility of the proposed fluorescence ELISA for HBsAg detection. Additionally, the developed method showed no false positive results analyzing 35 real HBsAg-negative serum samples, and exhibited excellent agreement (R=0.9907) with a commercial time-resolved fluorescence immunoassay (TRFIA) kit for detecting 31 HBsAg-positive serum samples. In summary, the proposed method based on fluorescence quenching of HO sensitive QDs is considerably to be an excellent biodetection platform with ultrahigh sensitivity, good accuracy and excellent reliability.
1408 related Products with: Fluorescence ELISA based on glucose oxidase-mediated fluorescence quenching of quantum dots for highly sensitive detection of Hepatitis B.Amplite™ Fluorimetric G Beta Amyloid (1 42) High Cell Meter™ Apoptotic a Amplite™ Fluorimetric L Amplite™ Fluorimetric F Amplite™ Fluorimetric G Amplite™ Fluorimetric G Highly Sensitive 8 OHdG C MarkerGeneTM Fluorescent 4 Nitro 7 (1 piperazinyl) Amplite™ Fluorimetric X Amplite™ Fluorimetric G
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Chronoamperometry-Based Redox Cycling for Application to Immunoassays.In this work, the chronoamperometry-based redox cycling of 3,3',5,5'-tetramethylbenzidine (TMB) was performed by using interdigitated electrode (IDE). The signal was obtained from two sequential chronoamperometric profiles: (1) with the generator at the oxidative potential of TMB and the collector at the reductive potential of TMB, and (2) with the generator at the reductive potential of TMB and the collector at the oxidative potential of TMB. The chronoamperometry-based redox cycling (dual mode) showed a sensitivity of 1.49 μA/OD, and the redox cycling efficiency was estimated to be 94% (n = 10). The sensitivities of conventional redox cycling with the same interdigitated electrode and chronoamperometry using a single working electrode (single mode) were estimated to be 0.67 μA/OD and 0.18 μA/OD, respectively. These results showed that the chronoamperometry-based redox cycling (dual mode) could be more effectively used to quantify the oxidized TMB than other amperometric methods. The chronoamperometry-based redox cycling (dual mode) was applied to immunoassays using a commercial ELISA kit for medical diagnosis of the human hepatitis B virus surface antigen (hHBsAg). Finally, the chronoamperometry-based redox cycling (dual mode) provided more than a 10-fold higher sensitivity than conventional chronoamperometry using a single working electrode (single mode) when applied to a commercial ELISA kit for medical diagnosis of hHBsAg.
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miR-29a promotes hepatitis B virus replication and expression by targeting SMARCE1 in hepatoma carcinoma.To investigate the functional role and underlying molecular mechanism of miR-29a in hepatitis B virus (HBV) expression and replication.
2936 related Products with: miR-29a promotes hepatitis B virus replication and expression by targeting SMARCE1 in hepatoma carcinoma.Human Epstein-Barr Virus High density breast invas Breast invasive ductal ca Breast invasive ductal ca Rabbit Anti-Hepatitis C V Breast cancer and matched Breast carcinoma tissue a Breast invasive ductal ca Multiple ovarian carcinom High density (188 cases 2 Breast fibroadenoma tissu Breast cancer and matched
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Investigation of a Novel Hepatitis B Virus Surface Antigen (HBsAg) Escape Mutant Affecting Immunogenicity.Mutation in the hepatitis B virus surface antigen (HBsAg) may affect the efficiency of diagnostic immunoassays or success of vaccinations using HBsAg. Thus, antigenicity and immunogenicity analyses of the mutated HBsAg are necessary to develop novel diagnostic tools and efficient vaccinations. Here, the in vitro antigenicity of three wild-type HBsAg open reading frames (ORFs) (adr4, W1S [subtype adr] and W3S [subtype adr]) isolated from clinically infected patients and nineteen synthesized single/double/multiple amino acid-substituted mutants were tested with commercial ELISA kits. Immunofluorescence staining of transfected cells and Western blot analysis confirmed that these ORFs were expressed at comparable levels in HEK-293 cells. W1S and adr4 were clearly detected, whereas W3S could not be detected. Using the same commercial immunoassay kit, we found that the single mutants, K120P and D123T, were marginally reactive, whereas W3S-aW1S and the double mutant, K120P/D123T, exhibited antigenicity roughly equivalent to the wild-type wako1S. On the other hand, the single mutants of W1S, P120K and T123D, significantly impaired the reactivity, while W1S-aW3S and the double mutant of W1S, P120K/T123D, resulted in a complete loss of antigenicity. In addition, ELISA revealed reduced HBs antigenicity of two mutants, W1S N146G and W1S Q129R/G145R. These commercial ELISA-based antigenic reactivities of HBsAg were also strongly correlated with the predicted Ai alterations of affected amino acids due to the specific mutation. In conclusion, this study showed for the first time that lysine (K120) and aspartate (D123) simultaneously affected HBsAg antigenicity, leading to diagnostic failure. These findings will improve diagnostic assays and vaccine development.
2096 related Products with: Investigation of a Novel Hepatitis B Virus Surface Antigen (HBsAg) Escape Mutant Affecting Immunogenicity.Human Hepatitis B Surface Anti-Hepatitis B Surface Anti-Hepatitis B Surface anti HBsAg surface antige Recombinant Hepatitis B S Hepatitis B surface Ag (H Human Anti-E Antigen of H HBV surface recombinant a Rabbit Anti-Hepatitis C V Hepatitis B surface Ag (H Rabbit Anti-Polyprotein(H Hepatitis B surface Ag (H
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Hepatitis B Virus Infections and Associated Factors among Pregnant Women Attending Antenatal Care Clinic at Deder Hospital, Eastern Ethiopia.Hepatitis B virus (HBV) infection is a serious public health problem worldwide. Reports have shown that 68,600 people die of HBV infection and more than 300,000 deaths due to liver cancer secondary to hepatitis B every year globally. Women who are infected with HBV can vertically transmit the infection to their infants. This study aims to determine the prevalence of HBV infection and associated factors among pregnant women.
1164 related Products with: Hepatitis B Virus Infections and Associated Factors among Pregnant Women Attending Antenatal Care Clinic at Deder Hospital, Eastern Ethiopia.Rabbit Anti-Polyprotein(H Rabbit Anti-Hepatitis C V Rabbit Anti-Hepatitis C V Hepatitis A Virus antibod Breast disease spectrum t Mouse Epstein-Barr Virus Lung cancer tissue array Colon cancer tissue array Rabbit Anti-Polyprotein(H Mid advanced stage bladde HbcAg - Hepatitis B Viru Anti-BRSV(Bovine Respirat
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