Only in Titles

           Search results for: Human IgG ELISA ELISA    

paperclip

#29677221   // Save this To Up

A 2015 outbreak of flea-borne rickettsiosis in San Gabriel Valley, Los Angeles County, California.

Although flea-borne rickettsiosis is endemic in Los Angeles County, outbreaks are rare. In the spring of 2015 three human cases of flea-borne rickettsiosis among residents of a mobile home community (MHC) prompted an investigation. Fleas were ubiquitous in common areas due to presence of flea-infested opossums and overabundant outdoor cats and dogs. The MHC was summarily abated in June 2015, and within five months, flea control and removal of animals significantly reduced the flea population. Two additional epidemiologically-linked human cases of flea-borne rickettsiosis detected at the MHC were suspected to have occurred before control efforts began. Molecular testing of 106 individual and 85 pooled cat fleas, blood and ear tissue samples from three opossums and thirteen feral cats using PCR amplification and DNA sequencing detected rickettsial DNA in 18.8% of the fleas. Seventeen percent of these cat fleas tested positive for R. felis-specific DNA compared to under two (<2) percent for Candidatus R. senegalensis-specific DNA. In addition, serological testing of 13 cats using a group-specific IgG-ELISA detected antibodies against typhus group rickettsiae and spotted fever group rickettsiae in six (46.2%) and one (7.7%) cat, respectively. These results indicate that cats and their fleas may have played an active role in the epidemiology of the typhus group and/or spotted fever group rickettsial disease(s) in this outbreak.

1208 related Products with: A 2015 outbreak of flea-borne rickettsiosis in San Gabriel Valley, Los Angeles County, California.

Rabbit Anti-Inf A Neurami Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti ING1B antisense AKT1 (dn) Inducible HIV 1 intergase antigen. Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon anti HSV (II) gB IgG1 (mo anti HCMV IE pp65 IgG1 (m

Related Pathways

paperclip

#29673623   // Save this To Up

Detection of serum antibodies in children and adolescents against Balamuthia mandrillaris, Naegleria fowleri and Acanthamoeba T4.

The presence of free-living amoebae of the genera Naegleria, Acanthamoeba and Balamuthia, which contain pathogenic species for humans and animals, has been demonstrated several times and in different natural aquatic environments in the northwest of Mexico. With the aim of continuing the addition of knowledge about immunology of pathogenic free-living amoebae, 118 sera from children and adolescents, living in three villages, were studied. Humoral IgG response against B. mandrillaris, N. fowleri and Acanthamoeba sp. genotype T4, was analyzed in duplicate to titers 1: 100 and 1: 500 by enzyme-linked immunosorbent assay (ELISA). Children and adolescents ages ranged between 5 and 16 years old, with a mean of 9 years old, 55% males. All tested sera were positive for the 1: 100 dilution, and in the results obtained with the 1: 500 dilution, 116 of 118 (98.3%) were seropositive for N. fowleri, 101 of 118 (85.6%) were seropositive for Acanthamoeba sp. genotype T4, and 43 of 118 (36.4%) were seropositive for B. mandrillaris. The statistical analysis showed different distributions among the three communities and for the three species of pathogenic free-living amoebae, including age. Lysed and complete cells used as Balamuthia antigens gave differences in seropositivity.

2922 related Products with: Detection of serum antibodies in children and adolescents against Balamuthia mandrillaris, Naegleria fowleri and Acanthamoeba T4.

Bovine Androstenedione,AS Rabbit Anti-Human Androge Goat Anti-Human Androgen Rabbit Anti-Rat Androgen Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser HIV1 integrase antibody, Human Serum Albumin antib Human Serum Albumin antib Androgen Receptor (Phosph

Related Pathways

paperclip

#29663448   // Save this To Up

Seroprevalence, Molecular Epidemiology and Quantitation of Parvovirus B19 DNA levels in Iranian Blood Donors.

Human parvovirus B19 (B19) infection is common among blood donors, and healthy blood donors can transmit virus via transfusion. Due to resistance of B19 to viral inactivation methods, there is a potential concern regarding transfusion safety in blood products. We aimed to determine the seroprevalence, molecular epidemiology and quantitation of B19 DNA levels in blood donors in Tehran, Iran. 500 blood donors from Blood Transfusion Research Center were studied. ELISA was used for detection of B19 IgG and IgM and nested PCR was carried out for detection of B19 DNA. PCR products were subjected to direct sequencing. B19 viral load was determined by real time PCR. B19 IgG, IgM and DNA were detected in 27.6%, 2.6% and 1.2% of donors respectively. 10 samples (2%) were positive for both antibodies while in 4 cases (0.8%), B19 IgG and DNA detected simultaneously. One case had B19 IgM, IgG and viremia concurrently. The titers of B19 DNA in 4 of 6 donors were more than 10 IU/ml (high level viremia) and all 4 cases had IgG simultaneously. All B19 isolates categorized in genotype 1A. Our findings indicated that prevalence of B19 DNA in Iranian blood donors was comparable with previous studies throughout the world. High level B19 viremia found in 0.8% of our donors and all viremic donors revealed neutralizing B19 antibody. Therefore implementation of a B19 screening test for each volunteer blood donor does not appear to be necessary but B19 testing for plasma-derived products seems important in Iranian donors. This article is protected by copyright. All rights reserved.

1621 related Products with: Seroprevalence, Molecular Epidemiology and Quantitation of Parvovirus B19 DNA levels in Iranian Blood Donors.

Human Parvovirus (B19 ) R DNA (cytosine 5) methyltr anti H inh human blood an C Peptide ELISA Kit, Rat Anti beta3 AR Human, Poly removed without changing BKV DNA quantitation BKV DNA quantitation BKV DNA quantitation BKV DNA quantitation Mouse Anti-Parvovirus B19 Pfu DNA Polymerase (Not a

Related Pathways

paperclip

#29663439   // Save this To Up

Sero-prevalence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) specific antibodies in Dromedary Camels in Tabuk, Saudi Arabia.

The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a novel Coronavirus which was responsible of the first case of human acute respiratory syndrome in the Kingdom of Saudi Arabia (KSA), 2012. Dromedary camels are considered as potential reservoirs for the virus and seem to be the only animal host which may transmit the infection to human. Further studies are required to better understand the animal sources of zoonotic transmission route and the risks of this infection. A primary sero-prevalence study of MERS-CoV preexisting neutralizing antibodies in Dromedary camel serum was conducted in Tabuk, western north region of KSA, in order to assess the seopositivity of these animals and to explain their possible role in the transmission of the infection to Human. One hundred seventy one (171) serum samples were collected from healthy dromedary camels with different ages and genders in Tabuk city and tested for specific serum IgG by ELISA using the receptor-binding S1 subunits of spike proteins of MERS-CoV. 144 (84,21%) of the total camel sera shown the presence of protein-specific antibodies against MERS-CoV. These results may provide evidence that MERS-CoV has previously infected dromedary camels in Tabuk and may support the possible role of camels in the human infection. This article is protected by copyright. All rights reserved.

2906 related Products with: Sero-prevalence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) specific antibodies in Dromedary Camels in Tabuk, Saudi Arabia.

Apoptosis antibody array Cell cycle antibody array Cytokine antibody array i Signal transduction antib AKT Phospho-Specific Arra AKT PKB Signaling Phospho AMPK Signaling Phospho-Sp Apoptosis Phospho-Specifi Cancer Apoptosis Phospho- Cell Cycle Control Phosph Cell Cycle Phospho-Specif Chromatin Transcription P

Related Pathways

paperclip

#29657714   // Save this To Up

Non-typeable detection in the lower airways of patients with lung cancer and chronic obstructive pulmonary disease.

Chronic airway inflammation and hypersensitivity to bacterial infection may contribute to lung cancer pathogenesis. Previous studies have demonstrated that nontypeable (NTHi) is the most common colonizing bacteria in the lower airways of patients with COPD. The objective of this study was to determine the presence of NTHi and immunoglobulin concentrations in patients with lung cancer, COPD and controls.

2103 related Products with: Non-typeable detection in the lower airways of patients with lung cancer and chronic obstructive pulmonary disease.

Lung disease spectrum (pu Lung disease spectrum (pu Anti 3 DG imidazolone Mon Lung disease spectrum tis Lung cancer tissue array, Lung cancer tissue array Rectum disease spectrum ( Kidney disease spectrum ( Breast disease spectrum t Colon cancer and lung can Brain disease spectrum (b Colon disease spectrum (c

Related Pathways

paperclip

#29656134   // Save this To Up

Seroprevalence and risk factors of Hepatitis E virus infection in cancer patients in eastern China.

Hepatitis E virus (HEV) is a single-stranded RNA virus infecting a variety of animals and humans. However, little is known of HEV infection among cancer patients in China. This study provides new epidemiological data for the prevalence of co-infection of HEV in cancer patients, indicating that HEV infection is common in this group.

2711 related Products with: Seroprevalence and risk factors of Hepatitis E virus infection in cancer patients in eastern China.

Human Epstein-Barr Virus Mouse Epstein-Barr Virus Infection diseases: Heli Infection diseases: Heli Breast cancer and matched Breast cancer tissue arra Breast cancer tissue arra Breast cancer tissue arra Endometrium cancer and no Endocrine cancer tissue a Esophagus cancer tissue a Esophageal cancer tissue

Related Pathways

paperclip

#29654554   // Save this To Up

Evaluation of Epstein-Barr Virus Salivary Shedding in HIV/AIDS Patients and HAART Use: A Retrospective Cohort Study.

Little data is available on the evaluation of the occurrence rates of Epstein-Barr virus (EBV) in saliva and relationship with highly active antiretroviral therapy (HAART) use in HIV/AIDS patients in China. We conducted a retrospective cohort study of EBV serological tests for HIV/AIDS patients who were treated in the hospitals for infectious diseases in Wuxi and Shanghai, China from May 2016 to April 2017. The EBV-seropositive samples were identified by ELISA. EBV-specific primers and probes were used for the quantitative detection of viral DNA from saliva via quantitative real-time polymerase chain reaction. CD4 cell counts of the HIV/AIDS patients were detected by a flow cytometry. A total of 372 HIV/AIDS patients were ultimately selected and categorized for this retrospective cohort study. For EBV IgG and IgM, the HIV/AIDS HAART use (H) and non-HAART use (NH) groups had significantly higher seropositive rates than the HIV-negative control group. The HIV/AIDS (NH) group had the highest seropositive rate (IgG, 94.27%; IgM, 68.98%) and the highest incidence of EBV reactivation or infection. For salivary EBV DNA-positive rates and quantities, the HIV/AIDS (H) (73.69%) and the HIV/AIDS (NH) (100%) groups showed significantly higher values than the HIV-negative control group (35.79%, > twofold). Further, the salivary EBV DNA-negative population had significantly higher CD4 cell counts than the EBV DNA-positive population in the HIV/AIDS (H) group and the HIV/AIDS (NH) groups. Thus, HAART use is beneficial in decreasing the EBV salivary shedding in HIV/AIDS patients and indirectly decreases EBV transmission risk.

2842 related Products with: Evaluation of Epstein-Barr Virus Salivary Shedding in HIV/AIDS Patients and HAART Use: A Retrospective Cohort Study.

Human Epstein-Barr Virus Mouse Epstein-Barr Virus HIV 1 intergase antigen. HA (Influenza A Virus Hem Epstein Barr Virus VCA Ig Epstein Barr Virus VCA Ig Epstein Barr Virus VCA Ig Anti-Infectious Pancreati Anti-Infectious Pancreati Anti-Infectious Pancreati Anti-Infectious Pancreati Avian Influenza virus (H7

Related Pathways

paperclip

#29648491   // Save this To Up

Sublingual administration of 2-hydroxyethyl methacrylate enhances antibody responses to co-administered ovalbumin and Streptococcus mutans.

The oral mucosa of patients undergoing dental procedures is often exposed to residual monomers leaking from incompletely cured acrylic resins. We investigated whether 2-hydroxyethyl methacrylate (HEMA) monomers applied to the sublingual mucosa in mice modulate the antibody responses towards co-administered ovalbumin (OVA) or live oral bacteria.

1573 related Products with: Sublingual administration of 2-hydroxyethyl methacrylate enhances antibody responses to co-administered ovalbumin and Streptococcus mutans.

Shiga Toxin 1 antibody, M Shiga Toxin 2 antibody, M Cholera toxin antibody, M Clostridium botulinum D T Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Diphtheria toxin antibody

Related Pathways

paperclip

#29645287   // Save this To Up

Seroprevalence, Molecular Epidemiology and Quantitation of Parvovirus B19 DNA levels in Iranian Blood Donors.

Human parvovirus B19 (B19) infection is common among blood donors, and healthy blood donors can transmit virus via transfusion. Due to resistance of B19 to viral inactivation methods, there is a potential concern regarding transfusion safety in blood products. We aimed to determine the seroprevalence, molecular epidemiology and quantitation of B19 DNA levels in blood donors in Tehran, Iran. 500 blood donors from Blood Transfusion Research Center were studied. ELISA was used for detection of B19 IgG and IgM and nested PCR was carried out for detection of B19 DNA. PCR products were subjected to direct sequencing. B19 viral load was determined by real time PCR. B19 IgG, IgM and DNA were detected in 27.6%, 2.6% and 1.2% of donors respectively. 10 samples (2%) were positive for both antibodies while in 4 cases (0.8%), B19 IgG and DNA detected simultaneously. One case had B19 IgM, IgG and viremia concurrently. The titers of B19 DNA in 4 of 6 donors were more than 10 IU/ml (high level viremia) and all 4 cases had IgG simultaneously. All B19 isolates categorized in genotype 1A. Our findings indicated that prevalence of B19 DNA in Iranian blood donors was comparable with previous studies throughout the world. High level B19 viremia found in 0.8% of our donors and all viremic donors revealed neutralizing B19 antibody. Therefore implementation of a B19 screening test for each volunteer blood donor does not appear to be necessary but B19 testing for plasma-derived products seems important in Iranian donors. This article is protected by copyright. All rights reserved.

2645 related Products with: Seroprevalence, Molecular Epidemiology and Quantitation of Parvovirus B19 DNA levels in Iranian Blood Donors.

Human Parvovirus (B19 ) R DNA (cytosine 5) methyltr anti H inh human blood an C Peptide ELISA Kit, Rat Anti beta3 AR Human, Poly removed without changing BKV DNA quantitation BKV DNA quantitation BKV DNA quantitation BKV DNA quantitation Mouse Anti-Parvovirus B19 Pfu DNA Polymerase (Not a

Related Pathways

paperclip

#29628427   // Save this To Up

External apical root resorption diagnosis by using FII human dentine fraction and salivary IGg.

External apical root resorption as a consequence of orthodontic treatment is an inflammatory pathological process that results in permanent loss of tooth structure from the root apex.

1307 related Products with: External apical root resorption diagnosis by using FII human dentine fraction and salivary IGg.

Rabbit anti human antipla Rabbit anti human antipla Rabbit anti human antipla Rabbit anti human antipla Rabbit anti human antipla Antibodies, Rabbit: Rabb Antibodies, Rabbit: Rabb Proteins and Antibodies H Antibodies, Rabbit: Rabb Antibodies, Rabbit: Rabb Proteins and Antibodies H Antibodies, Rabbit: Rabb

Related Pathways