Search results for: Human IgG Human ELISA Kit
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[Evaluation of ELISA kit for detection of serum specific IgG antibodies againstin diagnosis of human cysticercosis].To evaluate the ELISA kit for detection of IgG antibodies againstcysticercus in humans, so as to provide a reference for its application in clinical practice.
2637 related Products with: [Evaluation of ELISA kit for detection of serum specific IgG antibodies againstin diagnosis of human cysticercosis].Human Serum Albumin antib Human Serum Albumin antib ELMGHI Mouse IgG anti hum ELRGHI Rat IgG anti human ELHGCI Human Monkey IgG a ELHGBI Human Monkey IgG a ELHGPI Human Monkey IgG a ELHGHI Human Monkey IgG a Human IgG (total) ELISA K Human Neuron-specific eno ELMGHII Mouse IgG anti hu ELRGHII Rat IgG anti huma
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The First Serological Study of Q Fever in Humans in Lebanon.The aim of this study was to estimate, for the first time, the human seroprevalence of Q fever in Lebanon, by assessing the presence of antibodies against the causative agent, Coxiella burnetii. A total number of 421 serum samples (226 females and 196 males) were collected in February 2015 from hospitals and laboratories dispersed in five Lebanese provinces: Akkar, Bekaa, Mount Lebanon, Nabatieh, and South Lebanon.
Native Influenza HA (B Qi Native Influenza HA (B Qi Native Influenza HA (B Qi Inflammation (Human) Quan Inflammation (Human) Quan Inflammation (Human) Quan Inflammation (Mouse) Quan Inflammation (Rat) Quanti Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu
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Immunoglobulin subclass in experimental murineinfection.The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.
Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser ING1B antisense ING1B sense Interferon γ p19 INK4D AKT1 (dn) Inducible Insulin
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Evaluation of a genus-specific ELISA and a commercial Aspergillus Western blot IgG® immunoblot kit for the diagnosis of aspergillosis in common bottlenose dolphins (Tursiops truncatus).Aspergillosis is a fungal infection with high mortality and morbidity rates. As in humans, its definitive diagnosis is difficult in animals, and thus new laboratory tools are required to overcome the diagnostic limitations due to low specificity and lack of standardization. In this study of common bottlenose dolphins (Tursiops truncatus), we evaluated the diagnostic performance of a new commercial immunoblot kit that had been initially developed for the serologic diagnosis of chronic aspergillosis in humans. Using this in a quantitative approach, we first established its positive cutoff within an observation cohort of 32 serum samples from dolphins with "proven" or "probable" diagnosis of aspergillosis and 55 negative controls. A novel enzyme-linked immunosorbent assay (ELISA) test was also developed for detecting anti-Aspergillus antibodies, and results were compared between the two assays. Overall, the diagnostic performance of immunoblot and ELISA were strongly correlated (P < .0001). The former showed lower sensitivity (65.6% versus 90.6%), but higher specificity (92.7% vs. 69.1%), with no cross-reaction with other fungal infections caused by miscellaneous non-Aspergillus genera. When assessing their use in a validation cohort, the immunoblot kit and the ELISA enabled positive diagnosis before mycological cultures in 42.9% and 33.3% subjects addressed for suspicion of aspergillosis, respectively. There was also significant impact of antifungal treatment on the results of the two tests (P < .05). In all, these new serological methods show promise in aiding in the diagnosis of aspergillosis in dolphins, and illustrate the opportunity to adapt commercial reagents directed for human diagnostics to detect similar changes in other animals.
1623 related Products with: Evaluation of a genus-specific ELISA and a commercial Aspergillus Western blot IgG® immunoblot kit for the diagnosis of aspergillosis in common bottlenose dolphins (Tursiops truncatus).Rat Visceral adipose spec Mouse anti-ovalbumin spec Goat anti-ovalbumin speci ELMGCI Mouse IgG anti chi ELMGBI Mouse IgG anti bov ELMGPI Mouse IgG anti por ELMGHI Mouse IgG anti hum ELMGMsI Mouse IgG anti mo ELRGCI Rat IgG anti chick ELRGBI Rat IgG anti bovin ELRGPI Rat IgG anti porci ELRGRI Rat IgG anti rat t
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Seroprevalence of toxoplasmosis and risk factors of Toxoplasma gondii infection among pregnant women in Sri Lanka: a cross sectional study.Toxoplasma gondii is an intracellular protozoan infecting humans and animals. Infection in adults usually causes mild disease but greater importance lies in preventing transplacental transmission which can cause major foetal anomalies and is vital to identify infection in pregnancy. Research on this regard in Sri Lanka is scarce and would be beneficial in developing antenatal care strategies for improved foetal outcome.
2698 related Products with: Seroprevalence of toxoplasmosis and risk factors of Toxoplasma gondii infection among pregnant women in Sri Lanka: a cross sectional study.Toxoplasma gondii GRA8, r Toxoplasma gondii MIC 3 r Toxoplasma gondii P24 (GR Toxoplasma gondii P29 (GR Toxoplasma gondii P30 (SA Liver cancer tissue array Liver tissue cancer tissu Hepatocellular carcinoma Toxoplasma gondii SAG1 an Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti ING1B antisense
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A Lateral Flow Rapid Test for Human Toxocariasis Developed Using ThreeRecombinant Antigens.Laboratory diagnosis of toxocariasis is still a challenge especially in developing endemic countries with polyparasitism. In this study, threerecombinant antigens, rTES-26, rTES-30, and rTES-120, were expressed and used to prepare lateral flow immunoglobulin G4 (IgG4) dipsticks. The concordance of the results of the rapid test (comprising three dipsticks) with a commercial IgG-enzyme-linked immunosorbent assay (ELISA) (Cypress Diagnostics, Belgium) was compared against the concordance of two other commercial IgG-ELISA kits (Bordier, Switzerland and NovaTec, Germany) with the Cypress kit. Usingpositive samples, the concordance of the dipstick dotted with rTES-26, rTES-30, and rTES-120 was 41.4% (12/29), 51.7% (15/29), and 72.4% (21/29), respectively. When positivity with any dipstick was considered as an overall positive rapid test result, the concordance with the Cypress kit was 93% (27/29). Meanwhile, when compared with the results of the Cypress kit, the concordance of IgG-ELISA from NovaTec and Bordier was 100% (29/29) and 89.7% (26/29), respectively. Specific IgG4 has been recognized as a marker of active infection for several helminthic diseases; therefore, the two non-concordant results of the rapid test when compared with the NovaTec IgG-ELISA kit may be from samples of people with non-active infection. All the three dipsticks showed 100% (50/50) concordance with the Cypress kit when tested with serum from individuals who were healthy and with other infections. In conclusion, the lateral flow rapid test is potentially a good, fast, and easy test for toxocariasis. Next, further validation studies and development of a test with the three antigens in one dipstick will be performed.
2594 related Products with: A Lateral Flow Rapid Test for Human Toxocariasis Developed Using ThreeRecombinant Antigens.LATERAL FLOW ASSAY EZ PAN MOUSE ANTI HUMAN CD19 RPE Rapid Microplate Assay K Bone Morphogenetic Protei Growth Differentiation Fa Mouse Anti-Human Bcl-10 [ Mouse Anti-Human CD65s [+ Mouse Anti-Human TREM-1, succinate-CoA ligase, GDP formin-like 1 antibody So succinate-CoA ligase, ADP Beta Amyloid (42) ELISA K
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Generation and Characterization of a Bispecific Antibody Targeting Both PD-1 and c-MET.Bispecific antibodies, BsAbs, are molecules with the ability to bind to two different epitopes on the same or different antigens. c-MET, cellular-mesenchymal to epithelial transition factor, is deregulated in many types of human malignancies. Abnormal c-MET activation in cancer correlates with poor prognosis. PD-1, programmed death-1, is an additional inhibitory receptor expressed by T cells. Blocking the interactions between PD-1 and PD-L1 has emerged as a promising immunotherapy for treating cancer.
2455 related Products with: Generation and Characterization of a Bispecific Antibody Targeting Both PD-1 and c-MET.Androgen Receptor (Phosph Androgen Receptor (Phosph Androgen Receptor (Ab-650 Androgen Receptor Antibod Anti-Androgen Receptor pr Androgen Receptor , Mouse Androstane-3a, 17b-diol 5 Androstane-3a,17b-diol Gl Androstenedione-19 Antibo Androgen Receptor Antibod Androgen Receptor (Phosph Androgen Receptor (Phosph
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Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens.Three recombinant antigens viz. arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T. canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T. canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T. canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii, Plasmodium vivax, Entamoeba histolytica, hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T. gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T. gondii, P. vivax and E. histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T. canis larvae.
2938 related Products with: Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens.Recombinant Viral antige Cathepsin L, human recomb Macrophage Colony Stimula Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle
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Optimized enzyme-linked immunosorbent assay for detecting cytomegalovirus infections during clinical trials of recombinant vaccines.In clinical trials of cytomegalovirus (CMV) glycoprotein B (gB) vaccines, CMV infection is detected by first depleting serum of anti-gB antibodies and then measuring anti-CMV antibodies with a commercially available enzyme-linked immunosorbent assay (ELISA) kit, with confirmation of positive findings by immunoblot.
2818 related Products with: Optimized enzyme-linked immunosorbent assay for detecting cytomegalovirus infections during clinical trials of recombinant vaccines.Bone Morphogenetic Protei Growth Differentiation Fa ReadiUse™ ABTS Solution Cell Meter™ Cell Viabil Cell Meter™ Phosphatidy Recombinant Cytomegalovir Recombinant Cytomegalovir Recombinant Cytomegalovir Recombinant Cytomegalovir Cell Meter™ JC 10 Mitoc Cell Meter™ JC 10 Mitoc Cell Meter™ NIR Mitocho
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Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum.Immunoglobulin G (IgG) represents the major fraction of antibodies in healthy adult human serum, and deviations from physiological levels are a generic marker of disease corresponding to different pathologies. Therefore, screening methods for IgG evaluation are a valuable aid to diagnostics. The present work proposes a rapid, automatic, and miniaturized method based on UV-vis micro-bead injection spectroscopy (μ-BIS) for the real-time determination of human serum IgG with label-free detection. Relying on attachment of IgG in rec-protein G immobilized in Sepharose 4B, a bioaffinity column is automatically assembled, where IgG is selectively retained and determined by on-column optical density measurement. A "dilution-and-shoot" approach (50 to 200 times) was implemented without further sample treatment because interferences were flushed out of the column upon sample loading, with minimization of carryover and cross-contamination by automatically discarding the sorbent (0.2 mg) after each determination. No interference from human serum albumin at 60 mg mLin undiluted sample was found. The method allowed IgG determination in the range 100-300 μg mL(corresponding to 5.0-60 mg mLin undiluted samples), with a detection limit of 33 μg mL(1.7 mg mLfor samples, dilution factor of 50). RSD values were < 9.4 and < 11.7%, for intra and inter-assay precision, respectively, while recovery values for human serum spiked with IgG at high pathological levels were 97.8-101.4%. Comparison to commercial ELISA kit showed no significant difference for tested samples (n = 8). Moreover, time-to-result decreased from several hours to < 5 min and analysis cost decreased 10 times, showing the potential of the proposed approach as a point-of-care method. Graphical abstract Micro-Bead Injection Spectroscopy method for real time, automated and label-free determination of total serum human Immunoglobulin G (IgG). The method was designed for Lab-on-Valve (LOV) platforms using a miniaturised protein G bioaffinity separative approach. IgG are separated from serum matrix components upon quantification with low non-specific binding in less than 5 min.
1463 related Products with: Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum.Sterile filtered goat se Sterile filtered goat se Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Growth Differentiation Fa Human Serum Albumin antib Human Insulin-like Growth Human Epstein-Barr Virus Human Gro g Macrophage In Human Insulin-like Growth Human Interferon-gamma IF Goat Anti-Human Synaptota
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