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           Search results for: Human Metallothionein-1M(MT1M) ELISA kit SpeciesHuman   

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#29048509   2017/10/19 Save this To Up

Plasma But Not Cerebrospinal Fluid Interleukin 7 and Interleukin 5 Levels Pre-Antiretroviral Therapy Commencement Predict Cryptococcosis-Associated Immune Reconstitution Inflammatory Syndrome.

Patients with human immunodeficiency virus/AIDS-associated cryptococcal meningitis (CM) frequently experience clinical deterioration, known as cryptococcosis-associated immune reconstitution inflammatory syndrome (C-IRIS), upon initiation of antiretroviral therapy (ART). The immunological mechanisms underlying C-IRIS are incompletely defined and no reliable predictive biomarkers exist. We investigated whether plasma or cerebrospinal fluid (CSF) levels of cytokines and chemokines predicted C-IRIS and are potential predictive biomarkers.

2936 related Products with: Plasma But Not Cerebrospinal Fluid Interleukin 7 and Interleukin 5 Levels Pre-Antiretroviral Therapy Commencement Predict Cryptococcosis-Associated Immune Reconstitution Inflammatory Syndrome.

CELLKINES Natural Human I Human Interleukin-4 IL-4 Human Interleukin-6 IL-6 Human Interleukin-17E (IL Human Interleukin-17F IL- Human Interleukin-17AF He Human Interleukin-29 IL-2 Human Interleukin-17A IL- Rat Anti-Mouse Interleuki Mouse Anti-Human Interleu Rat Anti-Mouse Interleuki Rat Anti-Mouse Interleuki

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#29037956   2017/10/17 Save this To Up

A simple and cost-effective assay for measuring anti-drug antibody in human patients treated with Adalimumab.

It has been reported that 90% of the anti-drug antibody (ADA) to Adalimumab in human patients bound to the TNF-binding area, resulted in the annual loss of responses to Adalimumab up to 24%. It is of urgency to develop a cost-effective and easy-to-use ADA diagnostic kit for diagnosis of potential drug-resistance in patients treated with Adalimumab in clinic hospitals to avoid the tremendous economic and human costs to patients and health-care providers. In this study, we reported the generations of mouse monoclonal and monkey polyclonal antibodies against Adalimumab as assay standards and positive quality controls respectively. A Bridging ELISA assay was successfully developed with a limit of detection (LOD) between 22-80ng/ml. The preliminary validation of assay was carried out first with 50 normal human sera, further validated by screening the ADA in 192 serum samples from monkeys treated with or without Adalimumab. Our data showed that the Bridging ELISA kit is very sensitive, highly specific and ready for study in human clinic trials.

2951 related Products with: A simple and cost-effective assay for measuring anti-drug antibody in human patients treated with Adalimumab.

Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Mouse anti-human type I c Rat anti-human type I col Rat anti-human type I col Human monkey anti-chick t Human monkey anti-chick t Human monkey anti-bovine Human monkey anti-bovine Human monkey anti-porcine Human monkey anti-porcine Human monkey anti-human t

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#29035863   2017/10/16 Save this To Up

Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens.

Three recombinant antigens viz. arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T. canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T. canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T. canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii, Plasmodium vivax, Entamoeba histolytica, hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T. gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T. gondii, P. vivax and E. histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T. canis larvae.

2467 related Products with: Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens.

Recombinant Viral antige Cathepsin L, human recomb Macrophage Colony Stimula Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interfe Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle Recombinant Human Interle

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#29032226   2017/10/16 Save this To Up

Maternal depressive symptoms linked to reduced fecal Immunoglobulin A concentrations in infants.

Secretory Immunoglobulin A (sIgA) plays a critical role to infant gut mucosal immunity. Delayed IgA production is associated with greater risk of allergic disease. Murine models of stressful events during pregnancy and infancy show alterations in gut immunity and microbial composition in offspring, but little is known about the stress-microbiome-immunity pathways in humans. We investigated differences in infant fecal sIgA concentrations according to the presence of maternal depressive symptoms during and after pregnancy. A subsample of 403 term infants from the Canadian Healthy Infant Longitudinal Development (CHILD) cohort were studied. Their mothers completed the Center of Epidemiologic Studies Depression scale when enrolled prenatally and again postpartum. Quantified by Immundiagnostik sIgA ELISA kit, sIgA from infant stool was compared across maternal depressive symptom categories using Mann-Whitney U-tests and logistic regression models that controlled for various covariates. Twelve percent of women reported clinically significant depressive symptoms only prenatally, 8.7% had only postpartum symptoms and 9.2% had symptoms both pre and postnatally. Infants born to mothers with pre and postnatal symptoms had significantly lower median sIgA concentrations than those in the reference group (4.4mg/g feces vs. 6.3mg/g feces; p=0.033). The odds for sIgA concentrations in the lowest quartile was three-fold higher (95% CI: 1.25 - 7.55) when mothers had pre and postnatal symptoms, after controlling for breastfeeding status, infant age, antibiotics exposure and other covariates. Postnatal symptoms were not associated with fecal sIgA, independently of breastfeeding status. Infants born to mothers with depressive symptoms appear to have lower fecal sIgA concentrations, predisposing them to higher risk for allergic disease.

1637 related Products with: Maternal depressive symptoms linked to reduced fecal Immunoglobulin A concentrations in infants.

Recombinant Human Interfe Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri Homogenizer for 24 sample Top five cancer tissue ar Goat Anti-Human TOM1L1 SR FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Rabbit Anti-Human Toll In Oral squamous cell cancer

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#29030180   2017/10/14 Save this To Up

Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples.

Under favorable growth conditions, Staphylococcus aureus infects and causes illnesses in humans and animals. Staphylococcal enterotoxins are considered the most important factor for food poisoning, with these toxins usually found in milk and its byproducts. This study investigated the presence of a classical enterotoxin of S. aureus (enterotoxin A) in raw cow milk samples obtained from Region 3 of Tehran. Sixty cow milk samples were collected from traditional dairy sales centers in the study site. Electrophoresis, high-performance liquid chromatography, sandwich ELISA, and direct ELISA were evaluated as methods for detecting toxins in the milk. A direct ELISA kit was designed, with the best factors, the most suitable densities, and the most optimal temperatures and temporal incubation conditions incorporated into the design. Among the evaluated methods, direct ELISA exhibited the highest accuracy in toxin detection. The designed kit achieved a correctness coefficient of 0.98 and detected enterotoxin A in 23% of the samples. It also exhibited a relative sensitivity and other features that are comparable to those of other bacterial detection techniques. Finally, the kit achieved good matching and repetition results, and no cross-reactions occurred between enterotoxins during the procedure.

2412 related Products with: Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples.

Directed In Vivo Angiogen Beta Amyloid (42) ELISA K GLP 1 ELISA Kit, Rat Gluc Beta Amyloid (1 40) ELISA Beta Amyloid (40) ELISA K Beta Amyloid (40) ELISA K Beta Amyloid (1 42) High Glucagon ELISA KIT, Rat G Beta Amyloid (1 42) ELISA Beta Amyloid (1 40) ELISA Homogenizer for 24 sample Homogenizer for 8 samples

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#28967239   2017/10/02 Save this To Up

Effect of martial arts training on IL-6 and other immunological parameters among Trinidadian subjects.

Persistent bouts of extended exercise and heavy training are associated with depressed immune cell function. It has recently been demonstrated that IL-6 is produced locally in contracting skeletal muscles and acts on a wide range of tissues. Larger amounts of IL-6 are produced in response to exercise than any other cytokines. Though the majority of existing data obtained following prolonged exercise, it remains to be explained the effect of martial arts training on IL-6 and other immunological parameters and associated changes to the duration of this type of exercise. IL-1α is produced mainly by activated macrophages, as well as neutrophils epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. This study aimed to evaluate the effect of martial arts training on IL-6 and other immunological parameters among Trinidadian subjects.

2381 related Products with: Effect of martial arts training on IL-6 and other immunological parameters among Trinidadian subjects.

Androgen Receptor (Ab 650 17β-Acetoxy-2α-bromo-5 3-O-Acetyl 5,14-Androstad 3-O-Acetyl-17-O-tert-buty 3β-O-Acetyl-androsta-5,1 Androsta-1,4,6-triene-3,1 (3β)-Androsta-5,16-diene Rabbit Anti-IL-4I1 Polycl Rabbit Anti-IL-4I1 Polycl Rabbit Anti-Rat Androgen 19 Hydroxy 4 androstene 3 4 Androstene 3,17 dione C

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#28959352   2017/09/29 Save this To Up

Production of Erythropoietin-Specific Polyclonal Antibodies.

Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require specifi c antibodies. High cost, inappropriate shipping (cold chain failures), reduced sensitivity and thus poor detection performance are common pitfalls of using commercial kits for performing immunological tests.

2788 related Products with: Production of Erythropoietin-Specific Polyclonal Antibodies.

Anti VGLUT-1 Rat, polyclo Legionella pneumophila an alpha 1 Acid Glycoprotein S100 Protein antibody, Po Viral antibodies, anti-H Anti RAGE (Receptor for A Anti PDX1 Polyclonal Anti Anti-AdipoR1 Human Polycl Anti Galectin(Gal-3) Huma Anti PPARgamma Human, Pol Anti beta3-AR Human, Poly Apoptosis antibody array

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#28954256   2017/09/27 Save this To Up

Development of a bifunctional nanobiosensor for screening and detection of chemokine ligand in colorectal cancer cell line.

Highly sensitive detection of chemokines in various biological matrices and its interaction with a natural receptor molecule has tremendous importance in cell signaling, medical diagnostics, and therapeutics. In this direction, we have designed the first bifunctional nanobiosensor for chemokine screening and detection in a single experimental setting. The sensor probe was fabricated by immobilizing CXCR2 on the gold nanoparticles (AuNPs) deposited 2,2':5',2''-terthiophene-3' (p-benzoic acid) (TBA) nanocomposite film. The interaction between CXCR2 and chemokines was studied using electrochemical impedance spectroscopy (EIS) and voltammetry. CXCL5 among three ligands showed the strongest affinity to CXCR2, which was further utilized to develop an amperometric CXCL5 biosensor. Analytical parameters, such as CXCR2 receptor concentration, temperature, pH, and incubation time were optimized to obtain the high sensitivity. A dynamic range for CXCL5 detection was obtained between 0.1 and 10ng/mL with the detection limit of 0.078 ± 0.004ng/mL (RSD < 4.7%). The proposed biosensor was successfully applied to detect CXCL5 in clinically relevant concentrations in human serum and colorectal cancer cells samples with high sensitivity and selectivity. Interference effect and the stability of the developed biosensor were also evaluated. Method verification was performed by comparing the results using commercially available ELISA kit for CXCL5 detection.

1667 related Products with: Development of a bifunctional nanobiosensor for screening and detection of chemokine ligand in colorectal cancer cell line.

Bladder cancer tissue arr Breast cancer tissue arra Breast cancer tissue arra Cervix cancer tissue arra Esophagus cancer tissue a Esophageal cancer tissue Kidney cancer tissue arra Kidney cancer tissue arra Small cell lung carcinoma Non small cell lung carci Lung non small cell cance Non small cell lung carci

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#28953230   2017/09/27 Save this To Up

A Novel Fully Human Agonistic Single Chain Fragment Variable Antibody Targeting Death Receptor 5 with Potent Antitumor Activity In Vitro and In Vivo.

Agonistic antibodies, which bind specifically to death receptor 5 (DR5), can trigger apoptosis in tumor cells through the extrinsic pathway. In this present study, we describe the use of a phage display to isolate a novel fully human agonistic single chain fragment variable (scFv) antibody, which targets DR5. After five rounds of panning a large (1.2 × 10⁸ clones) phage display library on DR5, a total of over 4000 scFv clones were screened by the phage ELISA. After screening for agonism in a cell-viability assay in vitro, a novel DR5-specific scFv antibody TR2-3 was isolated, which inhibited COLO205 and MDA-MB-231 tumor cell growth without any cross-linking agents. The activity of TR2-3 in inducing apoptosis in cancer cells was evaluated by using an Annexin V-PE apoptosis detection kit in combination with flow cytometry and the Hoechst 33342 and propidium iodide double staining analysis. In addition, the activation of caspase-dependent apoptosis was evaluated by Western blot assays. The results indicated that TR2-3 induced robust apoptosis of the COLO205 and MDA-MB-231 cells in a dose-dependent and time-dependent manner, while it remarkably upregulated the cleavage of caspase-3 and caspase-8. Furthermore, TR2-3 suppressed the tumor growth significantly in the xenograft model. Taken together, these data suggest that TR2-3 exhibited potent antitumor activity both in vitro and in vivo. This work provides a novel human antibody, which might be a promising candidate for cancer therapy by targeting DR5.

2002 related Products with: A Novel Fully Human Agonistic Single Chain Fragment Variable Antibody Targeting Death Receptor 5 with Potent Antitumor Activity In Vitro and In Vivo.

Interferon-a Receptor Typ Human integrin aVb3, affi Rabbit Anti-Cell death in Rabbit Anti-Cell death in Anti AGO2 Human, Monoclon Mouse Anti-Human Interleu Integrin β1 (CD29) Antib LPAM-1(Integrin α4, CD49 α-Internexin Antibody So interleukin 17 receptor C interferon-alpha receptor Cytokine (Human) Antibody

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#28951710   2017/09/27 Save this To Up

Brain-derived neurotrophic factor is increased in serum levels of patients with symptomatic dermographism.

Symptomatic dermographism (SD) is the most common form of physical urticaria. However, the role of neuroimmune mechanisms in SD is unclear.

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Human Brain Derived Neuro Dog Brain-derived neurotr ELISA Kit for Brain Deriv Brain derived Neurotrophi Anti-Human Brain-Derived Anti Human Brain Derived Monoclonal Anti-Brain-der Monoclonal Anti Brain der Brain Derived Neurotrophi Brain Derived Neurotrophi Human Glial Derived Neuro Human Brain derived neuro

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