Search results for: Human premicroRNA Expression Construct LentimiR147b
#29297389 
2017/09/20
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A novel method to identify pre-microRNA in various species knowledge base on various species.
More than 1/3 of human genes are regulated by microRNAs. The identification of microRNA (miRNA) is the precondition of discovering the regulatory mechanism of miRNA and developing the cure for genetic diseases. The traditional identification method is biological experiment, but it has the defects of long period, high cost, and missing the miRNAs that but also many other algorithms only exist in a specific period or low expression level. Therefore, to overcome these defects, machine learning method is applied to identify miRNAs.Tianyi Zhao, Ningyi Zhang, Ying Zhang, Jun Ren, Peigang Xu, Zhiyan Liu, Liang Cheng, Yang Hu
2635 related Products with: A novel method to identify pre-microRNA in various species knowledge base on various species.
100ul 100ul 100ul 100ul100 ug 50 ug 100ul100ug50ul 100ul 100ul 100ul
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#25161167 2014/08/26
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Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells.
Noncoding microRNAs (miRNAs or miRs) have been revealed as critical epigenetic factors in the regulation of various cellular processes, including drug metabolism and disposition. However, research on miRNA functions is limited to the use of synthetic RNA and recombinant DNA agents. Herein, we show that novel pre-miRNA-27b (miR-27b) agents can be biosynthesized in Escherichia coli using recombinant RNA technology, and recombinant transfer RNA (tRNA)/mir-27b chimera was readily purified to a high degree of homogeneity (>95%) using anion-exchange fast protein liquid chromatography. The tRNA-fusion miR-27b was revealed to be processed to mature miRNA miR-27b in human carcinoma LS-180 cells in a dose- and time-dependent manner. Moreover, recombinant tRNA/miR-27b agents were biologically active in reducing the mRNA and protein expression levels of cytochrome P450 3A4 (CYP3A4), which consequently led to lower midazolam 1'-hydroxylase activity. These findings demonstrate that pre-miRNA agents can be produced by recombinant RNA technology for functional studies.Mei-Mei Li, Wei-Peng Wang, Wen-Juan Wu, Min Huang, Ai-Ming Yu
1316 related Products with: Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells.
50 1 mg100 10 10 ug2µg100 µg2
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